FastQCFastQC Report
Sat 31 Dec 2022
SRR5846581.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846581.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107419
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCTCGTATGC76237.096509928411175No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCTCGTATGCCGTCT65316.079929993762742No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCT15261.4206052932907587No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTA9370.872285163704745No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTAT4810.44777925692847637No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCTCGT4050.3770282724657649No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCCTGTCTCTTATAC3960.3686498664109701No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCCTGTCTCT3740.34816931827702735No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCTCGTATGCC3040.28300393785084577No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTAACTCGTATGC2700.25135218164384326No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCTCGT2590.24111190757687187No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCTC2020.1880486692298383No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTACCTCGTATGC1860.17315372513242536No Hit
GCACAAGGGGTATCCTGCAATTTTGCTGAATGTATTTGTTAATGTGTAGAAT1720.16012064904718906No Hit
CTAATTGGGAATCCTACTGTGAAGCTGTCGTTGAGAACTTAGGTTGGCACGT1660.1545350450106592No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCTCGTATG1630.15174224299239425No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCT1590.14801850696804103No Hit
TTCACTGGGGACAAAGTAATCTGGATTTATACCTTTTAAAAACCATTTTGAC1570.14615663895586442No Hit
CAAGCTCTTGGCTCAAGCAATCCTTCTACCTCAACCCTTCCAAAGCACTGGG1540.1433638369375995No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCTC1500.13964010091324627No Hit
GTCTACGGGACTTCATGTGAATGACATACTTCACAAGTACACTCGATACCTT1500.13964010091324627No Hit
TTGCTTGGGCTAAAACTCACCTGAGTTGTAAAAAACTCCAGTTGACACAAAA1450.13498543088280474No Hit
CACATGGGGGCCTTATAAATATATACAATTATTATTTGTCAATTCAAAGTAA1450.13498543088280474No Hit
GTTAAGGGGAGGGGTGCCAAGTCCAAGGCCTCCCACTGGGAGTCATCGCTGA1440.13405449687671642No Hit
CAAGTACGGGAAGGTATTAGAAAAACCATTTCATAACTTTGTCAAAGTTAAA1420.13219262886453978No Hit
TTAGTGGGGCGCCGGGGGACTGAACCTTCACTACCTTTTTGTATTCCTTCAC1410.1312616948584515No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTTACCTACCTCGTATGCCGTCT1390.12939982684627488No Hit
GTGCTAGGGGTAAAAACTGGCCGAGTATTGGCTGTGTGGAAGACTAAAGCTT1360.12660702482800995No Hit
CGAACAGGGATGGGAAGAGTGTCTTAAGCCTAATATTAAATCAGTTTTGTTA1350.12567609082192163No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCTCGTAT1350.12567609082192163No Hit
TAACTGAGGGGAAAACAACTTGGTATTTAAGTATGAAGTGGATGAAATGTTT1340.12474515681583333No Hit
GTAAGCGGGAGATCAGGGGTGAGGCTGAGAGACTCTGGGTTTAGGGCTAGCC1340.12474515681583333No Hit
ATTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTATC1330.12381422280974502No Hit
GCAATGGGCTGACCATTTTATATGATTCTTTTTTTCTTTTTCATCAAACTTA1320.1228832888036567No Hit
GTCGAAGGGGACCCAGAGTGGAACGCGTGGCCTATGCAGGTGGATTCCTTCA1320.1228832888036567No Hit
AGTAGCGGGCTAGAAAACCTTCAGCCAGCCTCCAGTGCTGTCGAGAGACCAC1300.12102142079148008No Hit
CGGTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCT1300.12102142079148008No Hit
TTTAGCGGGGGGGGCCACGTTTGCGTCCGCGCCATCAGGCCCGAGATAGCGG1280.11915955277930347No Hit
TTTAATGGGGATGCTTCACCATTCATGAATCATGGAAATCTGTATGTCTAAG1280.11915955277930347No Hit
TATCTAGGGAAATTTGATATAGTAGGCACTGACAAAAGAGGTCAAATGCAGG1270.11822861877321517No Hit
ATCTGAAGACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTA1260.11729768476712685No Hit
CAAAGTGTATGTATTTTAGTGGAAAATACAGTAATAATCCTCATCATGCTGA1260.11729768476712685No Hit
CGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCTCG1260.11729768476712685No Hit
GTGTGCGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1240.11543581675495024No Hit
CTTTTCGGGTAGATTTGAAATACTGATTCTTCAAACTTAGTGGTTAAGAGTA1240.11543581675495024No Hit
TATCAGCGGGCCGGCGTAAAGAGTGTTTTAGATCACCCCCTCCCCAATAAAG1230.11450488274886193No Hit
GTCCCAGGGAACACTGAACTGACAATTAACAGCCCAATATCTACAATCAACC1230.11450488274886193No Hit
CGCTATGGGCCGATCCCGAAAGGGGAAGATTACAACATTGATAACCTGTCTT1220.11357394874277361No Hit
TTTCTTGGGAGTCTATTTTTAGAGGATTGATCTCTGGCACAACCAGCTTCCT1210.11264301473668532No Hit
TTTACTGGGAGGTCACAGAGGGATATGCTGCATGAGATAATTAGATCCACAT1200.111712080730597No Hit
GCCTTAGGGGAAAGCAAAGGAAGTGCTGGGTGTAAAGTTTGCATGATTCCAT1190.1107811467245087No Hit
TCTTACGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1190.1107811467245087No Hit
TAAGTAGGGAGAAGTAGCATGTTGCTGCCAGAATACAAGCATTGCTTTTGGC1190.1107811467245087No Hit
TGCCAGGTCTCCTATCTCTCCCAGTCCTAGCTGCTGGCATCACTATACTACT1180.10985021271842038No Hit
AACTTTGGGAGAACTACTCCTTTAGGAATTTCTATTCTAGTCTGGATAAAAA1170.10891927871233209No Hit
ATACCTGGGATGGATCTTCTGGGAGAAGGACTAAAACGATCCATGGACAAGT1170.10891927871233209No Hit
GGCGGAGGGGAAGACAACTGGGAATGCCACAGTGGACCACCTCTCCAAGTAC1170.10891927871233209No Hit
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCTCGTAT1170.10891927871233209No Hit
AGGTCAGGGCCCGCTGCGCGGCGTCAATGAGCGCAAAGTAGCGCGCAGACCC1160.10798834470624377No Hit
GGTGCGGGGATTCTCTAGGAACTCACTTTTGAGAGTATTTTTCTCTAACCCA1150.10705741070015547No Hit
GTCTGAGGGGCAAAAGCCTATGGATTCAAAAATAAACTAATTATAGTTGCAG1150.10705741070015547No Hit
TTTTACGGACTCTGATGATGCTCTTAAGTTTTTTACAGACTATATGAACTCC1140.10612647669406715No Hit
TGCATAGGGTGCGACAGGGATCACCAGACACACCAGGCAACAGAAGTTGAAG1140.10612647669406715No Hit
ACTTGCGGGTAAGAGGTCTGATTTTTTAATGGTATGTTTGTATTTTTGTTAT1130.10519554268797884No Hit
GTTGGCGGACTCACCAGACGCCTCAACCGCCTTTTCATCAATCGCCCACATC1120.10426460868189052No Hit
TATTACTGGGAAATGTCTGTTAAAAGAATGTATAAAATATTTCATTGTATTT1120.10426460868189052No Hit
AACCTAGGGCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAA1110.10333367467580223No Hit
ACTCCGGGGGATCTGAATGTCTCCTTTTCCCCCTCAGAGGGCAAAGGTGAAC1110.10333367467580223No Hit
CAGCTAGGGACTTTCCTCAAACTCAGTTTATATTTTTGTTTGAAATTAAAGC1100.10240274066971393No Hit
TAACATGGGTATACAGGGATGTCAGATCCATGTTCTAAAAAATTTTTGTAAT1100.10240274066971393No Hit
CCAGCAGGGATCCTCAGGAACAATTAGCAGCAATAAGCAAGCCTTTGAAAAG1100.10240274066971393No Hit
AGCTGAGAATCAAATCAAGAACTCAATTTCTTTTACAATAGCAGCAGAAAAA1100.10240274066971393No Hit
ATCCTTGGGGGCTGGAGAACAGCGTCCACGCAGTCCTGCCTGGGGTCAGGCC1090.10147180666362561No Hit
GCATCTGGGCAACAGATATTTTAGCAATGTTTTATTGAATTCTTTCAGAACA1090.10147180666362561No Hit
GAACTCGGGGGTTCTGTTTTTGGGTGCTATAGGCCGCATAGATTTCCTGCAG1080.10054087265753732No Hit
GTATAAGGGGTATAGAAATCATTAATAAATATAAATGTCAGGTTTAGGACTC1080.10054087265753732No Hit
TCGCAAGGGAGTGGCGACACAGGCACCCAGCTTTGGGGTGGTGCTGACGCTC1080.10054087265753732No Hit
ATCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCTCG1080.10054087265753732No Hit
AAATCAGGGTCCCATCCCTTCCCTAGGTCTACGCTATGAGAGAAAAGCTTAG1080.10054087265753732No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)