FastQCFastQC Report
Sat 31 Dec 2022
SRR5846580.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846580.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105905
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCTCGTATGC76697.2413955903876115No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCTCGTATGCCGTCT63876.030876729144044No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCT14761.3937019026486002No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTA9690.9149709645436948No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTAT4680.44190548132760493No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCCTGTCTCTTATAC4390.41452244936499694No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCTCGT4190.39563759973561213No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCCTGTCTCT3560.3361503234030499No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCTCGTATGCC3480.328596383551296No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTAACTCGTATGC2460.23228365044143337No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCTCGT2440.23039516547849487No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCTC1930.18223879892356357No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCTC1780.16807516170152495No Hit
GCACAAGGGGTATCCTGCAATTTTGCTGAATGTATTTGTTAATGTGTAGAAT1670.1576884944053633No Hit
TTGCTTGGGCTAAAACTCACCTGAGTTGTAAAAAACTCCAGTTGACACAAAA1650.1558000094424248No Hit
CACATGGGGGCCTTATAAATATATACAATTATTATTTGTCAATTCAAAGTAA1530.1444690996647939No Hit
TTAGTGGGGCGCCGGGGGACTGAACCTTCACTACCTTTTTGTATTCCTTCAC1520.1435248571833247No Hit
CAAGCTCTTGGCTCAAGCAATCCTTCTACCTCAACCCTTCCAAAGCACTGGG1510.14258061470185543No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCTCGTATG1500.1416363722203862No Hit
TTCACTGGGGACAAAGTAATCTGGATTTATACCTTTTAAAAACCATTTTGAC1480.13974788725744772No Hit
GTCTACGGGACTTCATGTGAATGACATACTTCACAAGTACACTCGATACCTT1460.13785940229450924No Hit
TAACTGAGGGGAAAACAACTTGGTATTTAAGTATGAAGTGGATGAAATGTTT1440.13597091733157074No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCT1420.13408243236863226No Hit
CACGTTGGGATGGCCCAAGGGCAACAGATGCTCGCAGGGCAGCTGCTGCCGA1400.1321939474056938No Hit
GATCCAGGGATGGCTGAATCATCCGCTACCTTCACGCCAATGGCGCCTCAAT1400.1321939474056938No Hit
CTAATTGGGAATCCTACTGTGAAGCTGTCGTTGAGAACTTAGGTTGGCACGT1400.1321939474056938No Hit
GTCGAAGGGGACCCAGAGTGGAACGCGTGGCCTATGCAGGTGGATTCCTTCA1390.13124970492422455No Hit
TTTAGCGGGGGGGGCCACGTTTGCGTCCGCGCCATCAGGCCCGAGATAGCGG1360.12841697747981684No Hit
GTTAAGGGGAGGGGTGCCAAGTCCAAGGCCTCCCACTGGGAGTCATCGCTGA1350.12747273499834758No Hit
GTCCCAGGGAACACTGAACTGACAATTAACAGCCCAATATCTACAATCAACC1330.1255842500354091No Hit
ATCTGAAGACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTA1320.12464000755393985No Hit
AATACTGGGGGTTTGTTCTTGCATTCGCAACTTGGCTAACTGGTGAAGAGGC1300.12275152259100137No Hit
CCTTGGGGGCATTTATCACATCTCCCTGCCTCCAGTTTCAGGTATCGTTTGA1270.11991879514659365No Hit
AGGTCAGGGCCCGCTGCGCGGCGTCAATGAGCGCAAAGTAGCGCGCAGACCC1260.1189745526651244No Hit
CGGTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCT1260.1189745526651244No Hit
GATCAAGGGAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTC1250.11803031018365517No Hit
TGCCAGGTCTCCTATCTCTCCCAGTCCTAGCTGCTGGCATCACTATACTACT1240.11708606770218592No Hit
TTTCTTGGGAGTCTATTTTTAGAGGATTGATCTCTGGCACAACCAGCTTCCT1240.11708606770218592No Hit
ATAACAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1230.11614182522071669No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCTCGTAT1230.11614182522071669No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTACCTCGTATGC1220.11519758273924743No Hit
GGACATGGGACCAAGAGTTTGGCCAGTGTGTGGGAGACTTGAACACCCCCCA1210.1142533402577782No Hit
GTTGGCGGACTCACCAGACGCCTCAACCGCCTTTTCATCAATCGCCCACATC1210.1142533402577782No Hit
AGATGTGGGGAGTCTGAGGTGCGGGGTCCTGGGGCCCGGCGCGGGTGGCCGC1210.1142533402577782No Hit
TCGCAAGGGAGTGGCGACACAGGCACCCAGCTTTGGGGTGGTGCTGACGCTC1210.1142533402577782No Hit
AACCTAGGGCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAA1210.1142533402577782No Hit
TATCCGGGGGCAGGAAAATTAACTTTAAAAGTCTTAAAGCTTTTGCCCAAGT1210.1142533402577782No Hit
GGCGGAGGGGAAGACAACTGGGAATGCCACAGTGGACCACCTCTCCAAGTAC1210.1142533402577782No Hit
GCAATGGGCTGACCATTTTATATGATTCTTTTTTTCTTTTTCATCAAACTTA1200.11330909777630895No Hit
ACTTGCGGGTAAGAGGTCTGATTTTTTAATGGTATGTTTGTATTTTTGTTAT1190.11236485529483972No Hit
GCATCTGGGCAACAGATATTTTAGCAATGTTTTATTGAATTCTTTCAGAACA1190.11236485529483972No Hit
CAGCTAGGGACTTTCCTCAAACTCAGTTTATATTTTTGTTTGAAATTAAAGC1180.11142061281337048No Hit
TCTTACGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1180.11142061281337048No Hit
TTTACTGGGAGGTCACAGAGGGATATGCTGCATGAGATAATTAGATCCACAT1170.11047637033190123No Hit
CAAGTACGGGAAGGTATTAGAAAAACCATTTCATAACTTTGTCAAAGTTAAA1160.10953212785043198No Hit
ATCCAGGGGGTATCAGGCTGGGAGCGGCCTCCTCTGGCCTCCCCCATCAGTT1160.10953212785043198No Hit
ATTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTATC1160.10953212785043198No Hit
AAACAGGGGAACAGCCCAATATCTACAATCAACCAACAAGTCATTATTACCC1150.10858788536896274No Hit
CGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCTCG1150.10858788536896274No Hit
GCCTTAGGGGAAAGCAAAGGAAGTGCTGGGTGTAAAGTTTGCATGATTCCAT1150.10858788536896274No Hit
GTCTGAGGGGCAAAAGCCTATGGATTCAAAAATAAACTAATTATAGTTGCAG1150.10858788536896274No Hit
GTAAGCGGGAGATCAGGGGTGAGGCTGAGAGACTCTGGGTTTAGGGCTAGCC1150.10858788536896274No Hit
TGTAAGGGCAGGACGATCCTTTTTCCTTCAGCAGCATTTCTTACTGGCTGTG1140.10764364288749352No Hit
TAACATGGGATTTCTACTTGATGATGGGTTGCTGCTGCCAATGCTCCGTTTT1140.10764364288749352No Hit
ACCTTTGGGAACGCGAAAGTATGGGATACTTATAGATATATAGAAACATATA1140.10764364288749352No Hit
TAGTCAGGGCTGGGAAGCAGGGAGCGCTTCTGCCTGGCCGCCCCATCTGGGA1130.10669940040602427No Hit
AATTATGGGGTATTCAAATAATATTAAATAATATTCAGTAACTGAGGGAAGA1130.10669940040602427No Hit
AGCTGAGAATCAAATCAAGAACTCAATTTCTTTTACAATAGCAGCAGAAAAA1130.10669940040602427No Hit
CTCCAGATGGGAGAAGCTGAAAGAAAAGCTCAAGCCACTGATCAAGGTCATA1130.10669940040602427No Hit
CGCTATGGGCCGATCCCGAAAGGGGAAGATTACAACATTGATAACCTGTCTT1120.10575515792455503No Hit
TATCTAGGGAAATTTGATATAGTAGGCACTGACAAAAGAGGTCAAATGCAGG1120.10575515792455503No Hit
TAACATGGGTATACAGGGATGTCAGATCCATGTTCTAAAAAATTTTTGTAAT1120.10575515792455503No Hit
GTGCTAGGGGTAAAAACTGGCCGAGTATTGGCTGTGTGGAAGACTAAAGCTT1120.10575515792455503No Hit
AGATGGCATGTCTTTAGAGAAAAAAAAGACTTTTTTGGTTTAATTCATAAGG1110.10481091544308578No Hit
AACTTTGGGAGAACTACTCCTTTAGGAATTTCTATTCTAGTCTGGATAAAAA1110.10481091544308578No Hit
AACACAGGGATTATAACAAGCTCCATCTGCCTACGACAAACAGACCTAAAAT1110.10481091544308578No Hit
GCTATTGGGAGTATCAACAGTGTAATTCAGATTTTCTTATATCTACCGTACA1100.10386667296161654No Hit
GCTTTAGGGAGCTTTTCCAGCACCTCAGCCCCTTCACACATTTACACATACC1100.10386667296161654No Hit
TTGATAGGGCGCCCTGATCGGCGCACTGCGAGCAGTAGCCCAAACAATCTCA1090.10292243048014729No Hit
TTCACTGGGGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGC1090.10292243048014729No Hit
GGTACAGGGATCCAAAAGAATTGAAATCAGGATATAGAAGAGATATTTGCAC1080.10197818799867807No Hit
CGAACAGGGATGGGAAGAGTGTCTTAAGCCTAATATTAAATCAGTTTTGTTA1080.10197818799867807No Hit
ATCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCTCG1070.10103394551720882No Hit
TGGTGGGGGATCCCCAACTCCAGGGACCTCTTTATCTCAATCTATTTATTTG1070.10103394551720882No Hit
GAATATGGGAGACGTAAATTATGGCTGAATCATCCGCTACCTTCACGCCAAT1070.10103394551720882No Hit
ATACCTGGGATGGATCTTCTGGGAGAAGGACTAAAACGATCCATGGACAAGT1070.10103394551720882No Hit
ACTCCGGGGGATCTGAATGTCTCCTTTTCCCCCTCAGAGGGCAAAGGTGAAC1070.10103394551720882No Hit
GCATTTGGGAATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATT1060.10008970303573958No Hit
CGTACAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1060.10008970303573958No Hit
ACCCTGGGCCTCAACATCCCCCTCCTCTTCTACCACCTCTGGAGGTACTTCC1060.10008970303573958No Hit
ATCTATGGGGATTAATAAAACCTTAGATACTTAAATAAATCTTGTAAATCTA1060.10008970303573958No Hit
CTTTTCGGGTAGATTTGAAATACTGATTCTTCAAACTTAGTGGTTAAGAGTA1060.10008970303573958No Hit
AACGCTGGGAGCATTTAGCAATGATGGAAAGGATTCTTGGACCTCTACCAAA1060.10008970303573958No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)