FastQCFastQC Report
Sat 31 Dec 2022
SRR5846579.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846579.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105015
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCTCGTATGC73356.984716469075847No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCTCGTATGCCGTCT63226.0200923677569875No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCT15231.4502690091891635No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTA9410.8960624672665809No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTAT5000.4761224586963767No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCCTGTCTCTTATAC4070.3875636813788506No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCTCGT3900.37137551778317385No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCCTGTCTCT3810.36280531352663903No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTAACTCGTATGC3300.3142408227396086No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCTCGTATGCC2860.2723420463743275No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCTCGT2520.23996571918297385No Hit
TTGCTTGGGCTAAAACTCACCTGAGTTGTAAAAAACTCCAGTTGACACAAAA1850.17616530971765937No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCTCGTATG1840.17521306480026663No Hit
CACATGGGGGCCTTATAAATATATACAATTATTATTTGTCAATTCAAAGTAA1760.1675951054611246No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCTC1700.16188163595676808No Hit
TAACTGAGGGGAAAACAACTTGGTATTTAAGTATGAAGTGGATGAAATGTTT1650.1571204113698043No Hit
GCACAAGGGGTATCCTGCAATTTTGCTGAATGTATTTGTTAATGTGTAGAAT1630.15521592153501879No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCT1530.1456934723610913No Hit
CAAGCTCTTGGCTCAAGCAATCCTTCTACCTCAACCCTTCCAAAGCACTGGG1510.14378898252630576No Hit
ATTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTATC1430.13617102318716376No Hit
CGGTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCT1420.135218778269771No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCTC1390.1323620435175927No Hit
TTAGTGGGGCGCCGGGGGACTGAACCTTCACTACCTTTTTGTATTCCTTCAC1380.13140979860019997No Hit
TGCCAGGTCTCCTATCTCTCCCAGTCCTAGCTGCTGGCATCACTATACTACT1350.1285530638480217No Hit
GCAATGGGCTGACCATTTTATATGATTCTTTTTTTCTTTTTCATCAAACTTA1350.1285530638480217No Hit
CGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCTCG1350.1285530638480217No Hit
TTCACTGGGGACAAAGTAATCTGGATTTATACCTTTTAAAAACCATTTTGAC1330.1266485740132362No Hit
AATACTGGGGGTTTGTTCTTGCATTCGCAACTTGGCTAACTGGTGAAGAGGC1290.12283959434366519No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTACCTCGTATGC1270.12093510450887969No Hit
GTTGGCGGACTCACCAGACGCCTCAACCGCCTTTTCATCAATCGCCCACATC1250.11903061467409418No Hit
GTAAGCGGGAGATCAGGGGTGAGGCTGAGAGACTCTGGGTTTAGGGCTAGCC1250.11903061467409418No Hit
AACACAGGGATTATAACAAGCTCCATCTGCCTACGACAAACAGACCTAAAAT1250.11903061467409418No Hit
CTAATTGGGAATCCTACTGTGAAGCTGTCGTTGAGAACTTAGGTTGGCACGT1240.11807836975670143No Hit
TTTCTTGGGAGTCTATTTTTAGAGGATTGATCTCTGGCACAACCAGCTTCCT1240.11807836975670143No Hit
GTGCTAGGGGTAAAAACTGGCCGAGTATTGGCTGTGTGGAAGACTAAAGCTT1220.11617387992191593No Hit
GCATTTGGGAATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATT1210.11522163500452318No Hit
CAAAGTGTATGTATTTTAGTGGAAAATACAGTAATAATCCTCATCATGCTGA1210.11522163500452318No Hit
TATCAGCGGGCCGGCGTAAAGAGTGTTTTAGATCACCCCCTCCCCAATAAAG1210.11522163500452318No Hit
GTCTACGGGACTTCATGTGAATGACATACTTCACAAGTACACTCGATACCTT1210.11522163500452318No Hit
AGGTCAGGGCCCGCTGCGCGGCGTCAATGAGCGCAAAGTAGCGCGCAGACCC1190.11331714516973766No Hit
TATCTAGGGAAATTTGATATAGTAGGCACTGACAAAAGAGGTCAAATGCAGG1190.11331714516973766No Hit
ATCTGAAGACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTA1180.11236490025234491No Hit
CAAGTACGGGAAGGTATTAGAAAAACCATTTCATAACTTTGTCAAAGTTAAA1170.11141265533495216No Hit
ATACCTGGGATGGATCTTCTGGGAGAAGGACTAAAACGATCCATGGACAAGT1160.11046041041755941No Hit
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCTCGTAT1160.11046041041755941No Hit
CGCTATGGGCCGATCCCGAAAGGGGAAGATTACAACATTGATAACCTGTCTT1150.10950816550016663No Hit
TTTACTGGGAGGTCACAGAGGGATATGCTGCATGAGATAATTAGATCCACAT1150.10950816550016663No Hit
CTCCAGATGGGAGAAGCTGAAAGAAAAGCTCAAGCCACTGATCAAGGTCATA1150.10950816550016663No Hit
CGTACAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1140.10855592058277388No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCTCGTAT1140.10855592058277388No Hit
ATATGGGTGCTGGCACAATGACACTTAAAACCTGAAATTTTCAGGTCCACGT1140.10855592058277388No Hit
GGACATGGGACCAAGAGTTTGGCCAGTGTGTGGGAGACTTGAACACCCCCCA1130.10760367566538112No Hit
GAACTCGGGGGTTCTGTTTTTGGGTGCTATAGGCCGCATAGATTTCCTGCAG1130.10760367566538112No Hit
CCTCAAGGGACTTGAATCTGCATGACAGTTTGACTTGAATGCAACAGCAGGA1130.10760367566538112No Hit
TGTAAGGGCAGGACGATCCTTTTTCCTTCAGCAGCATTTCTTACTGGCTGTG1130.10760367566538112No Hit
TAAGCAGGGCTGCTGCAGGTCAGCAACTGTGTCGTGAGCAGCTGCCAACCAC1130.10760367566538112No Hit
AGTAGCGGGCTAGAAAACCTTCAGCCAGCCTCCAGTGCTGTCGAGAGACCAC1130.10760367566538112No Hit
ATCTATGGGGATTAATAAAACCTTAGATACTTAAATAAATCTTGTAAATCTA1130.10760367566538112No Hit
GTCGAAGGGGACCCAGAGTGGAACGCGTGGCCTATGCAGGTGGATTCCTTCA1120.10665143074798837No Hit
AAATCAGGGTCCCATCCCTTCCCTAGGTCTACGCTATGAGAGAAAAGCTTAG1110.10569918583059562No Hit
TATCCGGGGGCAGGAAAATTAACTTTAAAAGTCTTAAAGCTTTTGCCCAAGT1110.10569918583059562No Hit
TTTAGCGGGGGGGGCCACGTTTGCGTCCGCGCCATCAGGCCCGAGATAGCGG1100.10474694091320287No Hit
GTCTGAGGGGCAAAAGCCTATGGATTCAAAAATAAACTAATTATAGTTGCAG1100.10474694091320287No Hit
GTTAAGGGGAGGGGTGCCAAGTCCAAGGCCTCCCACTGGGAGTCATCGCTGA1100.10474694091320287No Hit
TTTTTGGGCAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTG1100.10474694091320287No Hit
AGATGGCATGTCTTTAGAGAAAAAAAAGACTTTTTTGGTTTAATTCATAAGG1090.10379469599581012No Hit
GCTATTGGGAGTATCAACAGTGTAATTCAGATTTTCTTATATCTACCGTACA1090.10379469599581012No Hit
AGTTCAGGGCTCTTAACTTTTTGAAAATATACAATAAATTACTAAACATATT1090.10379469599581012No Hit
GGCTGAGGGGGAAATATTAGTTCATGGATGGTGGTCATTTATTAAGACGTAA1090.10379469599581012No Hit
GATCCAGGGATGGCTGAATCATCCGCTACCTTCACGCCAATGGCGCCTCAAT1090.10379469599581012No Hit
GCCTTAGGGGAAAGCAAAGGAAGTGCTGGGTGTAAAGTTTGCATGATTCCAT1080.10284245107841736No Hit
TCCCTGGGGTCTCGTCTAGGGGAAAAAATAGTAAAGCGAAACTGCTGAAGGT1080.10284245107841736No Hit
CCAGCAGGGATCCTCAGGAACAATTAGCAGCAATAAGCAAGCCTTTGAAAAG1080.10284245107841736No Hit
CTTTTCGGGTAGATTTGAAATACTGATTCTTCAAACTTAGTGGTTAAGAGTA1080.10284245107841736No Hit
CAGCTAGGGAAAGAACAGTATAATGAACCCCTTTGAAACTCCACCCAGCTTC1070.10189020616102461No Hit
TAACATGGGATTTCTACTTGATGATGGGTTGCTGCTGCCAATGCTCCGTTTT1070.10189020616102461No Hit
GCATTCGGGGACCATGGAAGATAGTCTTATGTAAAGGTTAAATGGTGTTTAC1070.10189020616102461No Hit
TTAGAAGGGAGAGCTAAGTATCTGTGAGGCAATCTCTGCGAACGCTGAACTT1060.10093796124363186No Hit
CGAACAGGGATGGGAAGAGTGTCTTAAGCCTAATATTAAATCAGTTTTGTTA1060.10093796124363186No Hit
ATCACAGGGAGGAAGAGACTGAGCACAATTGATGAGCTGGATGAATTATTCC1060.10093796124363186No Hit
TCGCAAGGGAGTGGCGACACAGGCACCCAGCTTTGGGGTGGTGCTGACGCTC1060.10093796124363186No Hit
TTGTCGGGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGA1060.10093796124363186No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)