FastQCFastQC Report
Sat 31 Dec 2022
SRR5846566.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846566.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138654
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACTTCCGATCTCGTATGC5360.38657377356585454No Hit
CTTATACACATCTCCGAGCCCACGAGACCACTTCCGATCTCGTATGCCGTCT5050.36421596203499357No Hit
ACTATAGGGGAAAAGTGATTTCAACTTGAGTATTTTATGCAGGGTGGGAGAC2770.19977786432414502No Hit
CTTATACACATCTCCGAGCCCACGAGACCACTTCCGACTGTCTCTTATACAC2370.1709290752520663No Hit
GACTGCGGGGACGTCGGGAGCAGGATGGCGGCGGAGCAGGACCCCGAGGCGC2030.1464076045407994No Hit
CTCTGCGGGGCACCGTGGCAGATCACTTCAATTAAACCGTGGTGATAAAATC1800.12981955082435415No Hit
TAGCTTGGGAATTCTTAAACTGTTCCAAAAAAATTGAAGACAAAGGAATTTT1730.12477101273674036No Hit
TGACAGGGGAACTTGGCTCAGCTGCCGGGCTGCTCCGGTTGGAAACGCCAAG1720.12404979300993842No Hit
ACGAGGGGATTCAAGATTAACATAAATTTATTGTAGTCAAGAAAATGTAGTA1690.1218861338295325No Hit
ATGCGTGGGGAGTCATACATTTTTTTGGACTTTGTTATAATTCATTGTGGTA1670.12044369437592857No Hit
GTTGGCGGGAGGTGCCCAGACATCTAGAGGGGCGCTCAGCAGATGGGAGTCA1650.11900125492232463No Hit
ACGATTGGGGTGTAGTACTTCTGGGAAAGATCTCCTTGATACAACATTGTCA1610.11611637601511678No Hit
GCTCGCGGGAGGGAGACCGAAGGGCACAGCTCCCCGCGCCGCGCACGCCGCC1600.11539515628831479No Hit
GTACCAGGGGAGACCTAGCGCGTCCAGGTGGGAGGTTAGTGTGGCCCGGGCG1580.11395271683471087No Hit
ACTAGAGGGCAGCAAAGCATTGTCTTGAGCTAGTTCCTGGGGTGTGGAAGTA1580.11395271683471087No Hit
GTAGCAGGGGCGGGCAGATCGTTTGAGGTCGGGAGTTCGAGACCGGCCTGGC1580.11395271683471087No Hit
ACTAAAGGGGGACAGACTGCGGGACGGACGGTGGACGCTGGGACGCGTTTGT1570.1132314971079089No Hit
ATTACGGGGGATCTACGAGCGGGCAATCACTATACAAAATCTCAGAAATGTA1530.11034661820070102No Hit
GCTTGAGGGGGAGGGTATGGATATTTGCCTGAAGATAAAGTATTGGGAACTT1530.11034661820070102No Hit
TCTTATGGGCACTGGTATTTTAACTCTGCCTAATCTGTCTGATTTCAAGACC1520.10962539847389906No Hit
ATAAAAGGGAAGACACTGAAACCAGGAGTTTTAAATGGCTGGAACTCAGATG1510.10890417874709708No Hit
AAGCGTTGGGAGCCATTTTCCTTCCCAGATCAACCCCTGCCTCCCCCATGAC1490.10746173929349315No Hit
GCAAAAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1480.1067405195666912No Hit
AAAGCAGGGGTTTCAGGCCCTTCTTGTACTTTCTCTACCTTAGCCATGGAAT1480.1067405195666912No Hit
TACTTAGGGTTGTGAAATGTAATGAGATGTATCAAGATATCCTAGAAATAAA1470.10601929983988921No Hit
TACAAAGGGATTGCAGGCAGTGCCGTCCTGCTTTATGCTTTTGTGGAGACCC1470.10601929983988921No Hit
TTTGGTGGGTGACCTTCCTGGGCCGGGCCGCAGTGCTGGGTCAGTTCCAGCA1470.10601929983988921No Hit
GGATTAGGGTAAAAGGAAAAACTTGATATTTATCAGTCTGAGAAAATATTTT1460.10529808011308726No Hit
ACATTAGGGACATCAGTGATGAATGCAAGAGGCGTTTCTATAAGAATTGGCA1450.10457686038628528No Hit
TGTAAGTGGGACCTTAAGAAATTTCCTTCCATAGACAGCTGCCTCAAAGGGA1450.10457686038628528No Hit
TGCAGTGGGATGACATTGGGTAGCATTAACTGTAAGTTTTGTGCTTCCAAAT1450.10457686038628528No Hit
AAGTCCGGGACTATCAGCTGGGACAAGGGTTGGCAAACTATTGGCCATGGAC1450.10457686038628528No Hit
TTTAATGGGGGAGTTTAAAACACATTTATGTGTGTATGTATTTGAGAAGAGG1440.10385564065948333No Hit
TTGCTAGGGGTGAACCTTAGTAAGAAAGATAAGGTATTTAAGGGGCCTTTGA1440.10385564065948333No Hit
ATTCACGGGGAAAGAAGATAGTGTATGAGACTTAAGCCATGAGTTTTGTATC1420.10241320120587939No Hit
ATTCGAGGGAGAGGAGTGATTTCAAAGAAATCAGATTCTACACCATTAGATC1410.10169198147907742No Hit
ATAAGCGGGATCAAAGAGAGACTTCTAAGACAAAAAGCATCATTAATGAGGC1410.10169198147907742No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACTTCCGACTGTCTCTTA1410.10169198147907742No Hit
GCATGTGGGCACCTCTGAGCCTCCGATGCTCTGTGAAATGGAAGGAATGTGC1410.10169198147907742No Hit
AAAAGTGGGACTGCCAGATGGCCCCGCCTGTTAGGACCGCCTGCTCTACCCT1400.10097076175227544No Hit
ACGAGAGGGCTATAGTTCTTGTACATAGAATTTGCCCCATGAATATCTGTTA1400.10097076175227544No Hit
ATGTCTGGGCACGGAGGACATCTACCTGGAGTAGCCTGCCCACCGCTCTGTC1400.10097076175227544No Hit
TGAGCAGGGGAGGCTGTGGAGAGAGCGCGGAGACAAGCGCAGAGCGCAGCGC1390.10024954202547348No Hit
TAAACAGGGCACCATGCCGTATTAGACCTAAGTTCTTGCTCTTGGATGAAGT1390.10024954202547348No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)