FastQCFastQC Report
Sat 31 Dec 2022
SRR5846564.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846564.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137118
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCACTTCCGATCTCGTATGCCGTCT5180.3777768053793083No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACTTCCGATCTCGTATGC5100.3719424145626395No Hit
ACTATAGGGGAAAAGTGATTTCAACTTGAGTATTTTATGCAGGGTGGGAGAC2220.1619043451625607No Hit
CTTATACACATCTCCGAGCCCACGAGACCACTTCCGACTGTCTCTTATACAC1950.14221327615630333No Hit
GACTGCGGGGACGTCGGGAGCAGGATGGCGGCGGAGCAGGACCCCGAGGCGC1830.13346168993130003No Hit
AAAGCAGGGGTTTCAGGCCCTTCTTGTACTTTCTCTACCTTAGCCATGGAAT1830.13346168993130003No Hit
CTCTGCGGGGCACCGTGGCAGATCACTTCAATTAAACCGTGGTGATAAAATC1780.129815195670882No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACTTCCGACTGTCTCTTA1770.1290858968187984No Hit
GTGTCAGGGATTGCTTATGAAAATTCCATAGTGGTATTTTTTTGGATTCTTA1760.12835659796671478No Hit
ATGCGTGGGGAGTCATACATTTTTTTGGACTTTGTTATAATTCATTGTGGTA1700.12398080485421317No Hit
CACTGAGGGTACAACTAAAACATAATGATATCAAAAAGTTAAAAGGAAGGAA1670.12179290829796234No Hit
GTACCAGGGGAGACCTAGCGCGTCCAGGTGGGAGGTTAGTGTGGCCCGGGCG1670.12179290829796234No Hit
GTTGGCGGGAGGTGCCCAGACATCTAGAGGGGCGCTCAGCAGATGGGAGTCA1670.12179290829796234No Hit
TGTAAGTGGGACCTTAAGAAATTTCCTTCCATAGACAGCTGCCTCAAAGGGA1660.12106360944587874No Hit
ACTTATGGGTATTCATTTGCATGTGGGTATCTTATTTTTTCCAACACCATTT1640.11960501174171152No Hit
TAGACCGGGCTGCTCACCTTTGTCTTGGTAGTGTGAAAGCAAACTTAGATAA1610.11741711518546069No Hit
ACTAGAGGGCAGCAAAGCATTGTCTTGAGCTAGTTCCTGGGGTGTGGAAGTA1580.11522921862920987No Hit
ACGATTGGGGTGTAGTACTTCTGGGAAAGATCTCCTTGATACAACATTGTCA1580.11522921862920987No Hit
AAAAGTGGGACTGCCAGATGGCCCCGCCTGTTAGGACCGCCTGCTCTACCCT1560.11377062092504267No Hit
TGCTCTGGGAGGGATAAAGAAAATTAAGCCTGCCTCTAGGCACGTCTCAAAC1560.11377062092504267No Hit
GCGCAGGGGTAGGAGGACTGCAAGTCTAACGAAGTCTAGCCATGGCCCCTTT1560.11377062092504267No Hit
TTCGCTCGGGGAATGAGTGGGCTGTAGTTGGGCCTAAATAAATGAGCTGTTG1540.11231202322087544No Hit
GACAAAGGGCCCTGCTCCGTGGCTGGTTTTCTCCGCGGGCGCCTCGGGCGGA1510.11012412666462464No Hit
ATAAAAGGGAAGACACTGAAACCAGGAGTTTTAAATGGCTGGAACTCAGATG1510.11012412666462464No Hit
AAGCGTTGGGAGCCATTTTCCTTCCCAGATCAACCCCTGCCTCCCCCATGAC1490.10866552896045742No Hit
CTTTAGGGGACTATTGACTTAATTTGGTGAGATATGCTGATCAGTTGGACTA1480.10793623010837382No Hit
GCATGTGGGCACCTCTGAGCCTCCGATGCTCTGTGAAATGGAAGGAATGTGC1480.10793623010837382No Hit
TAAACAGGGCACCATGCCGTATTAGACCTAAGTTCTTGCTCTTGGATGAAGT1480.10793623010837382No Hit
GTTACCAAATTTCAGCTCTTAGGAGCTGCTCTACAATTCTGAATTTGCTTTT1470.10720693125629019No Hit
TAGCTTGGGAATTCTTAAACTGTTCCAAAAAAATTGAAGACAAAGGAATTTT1470.10720693125629019No Hit
TAAGCCGGGAGCTCTTGAAATCTTCTATATTTGCAAATGAATGTATTAGTGT1440.10501903470003938No Hit
TTCTCCCGGGACCAGTTTCTAATCATAGATACTATCTTCCATTTCATTGTAT1440.10501903470003938No Hit
TGCCAAGGGCCTCTGTGAAGTTGCGGCTGCTCTGCAGCCAGGTACTAAAGGA1420.10356043699587217No Hit
TTGCTAGGGATGGAAATACGAAATTGCAAATCTAAGTGAAAAACCTTTGTGT1420.10356043699587217No Hit
GGATTAGGGTAAAAGGAAAAACTTGATATTTATCAGTCTGAGAAAATATTTT1420.10356043699587217No Hit
GTAGCAGGGGCGGGCAGATCGTTTGAGGTCGGGAGTTCGAGACCGGCCTGGC1420.10356043699587217No Hit
ATTCGAGGGAGAGGAGTGATTTCAAAGAAATCAGATTCTACACCATTAGATC1410.10283113814378857No Hit
AATCATGGGAGGCCACCATGAGGGAGTTGTCCAAAGAAATGATGAAGGCTGG1410.10283113814378857No Hit
TTTTACGGGGGGTCAAGGACCAGGGAGCCCTGACTCCCAGAGGCTGCCACCG1400.10210183929170497No Hit
TTTAATGGGGGAGTTTAAAACACATTTATGTGTGTATGTATTTGAGAAGAGG1390.10137254043962134No Hit
AGTTGGGATGGCCTATCTCTTTACAATTTAATGAGAGCTGAGAAATTGTGGG1390.10137254043962134No Hit
TTGGCGGGGGGAGAGAAAGAGAGATGAAAACTGTGAGGGGTGAAACTCCACA1390.10137254043962134No Hit
CGTACTGGGAGCCATAGTAAGAGAAATATGAGTGCTATCAATACATGAATAT1380.10064324158753774No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)