FastQCFastQC Report
Sat 31 Dec 2022
SRR5846548.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846548.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29726
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACTTCCGATCTCGTATGC7462.5095875664401532No Hit
CTTATACACATCTCCGAGCCCACGAGACCACTTCCGATCTCGTATGCCGTCT7012.3582049384377313No Hit
CTTATACACATCTCCGAGCCCACGAGACCACTTCCGACTGTCTCTTATACAC1570.5281571688084505No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACTTCCGACTGTCTCTTA1380.46424005920742784No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACTTCCG1250.42050730000672815No Hit
ATTGGCGGGATGCTTTTTCTTGATGCTAAGATCACTGCAGAAGAATAACCTG1230.4137791832066205No Hit
ATGAAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1220.41041512480656667No Hit
ACGCTTGGGCTGAGCGCGAGGCTGCAAATTTCATCATGAAGGTCATCCAGAG1200.403687008006459No Hit
ATTCTGGGATTTGATGCTCTCTTGTATTTTAAATCTCATTTAAATCTTTGTC1180.39695889120635136No Hit
AAGTGCGGGCTTTGGAGGGGGTGGGGTCCGTTGGCATCAACACGGGGACCCT1100.37004642400592075No Hit
CACCAAGGGGGGAGTTGGGAGCAAAGCGGAGGCCCGGGCTGCCCGCCGTCCC1100.37004642400592075No Hit
GCCCAGCAGCAGAGCTCATGACAAACAGTAAACCCCAGCGAAAGGACCTGGC1090.3666823656058669No Hit
AGTCTCGGGATTCTCTCTTCAGGTATTCCTCCTTTGGTGTGGTGTCATTGAG1090.3666823656058669No Hit
ATATGTGGGCATGCTGATTGAGTGTTTTCCTGTACCATAGTAACTACATTGA1080.3633183072058131No Hit
AAATAAGGGTCCCAGAAGGACCTCCTGAGCGATGACTCAAGTGTCTCAGTCC1070.35995424880575927No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCACTTCCGA1060.35659019040570544No Hit
ATAGTTGGGTCCGATCTTGCTGTGGGTCTGTGAAAGGCACTTTAATTATCAC1010.33976989840543637No Hit
GCTCAAGGGGGGAGCAGGCGCTGCGGCAGGAGGGAAGATGGCGGACGAGGAG980.32967772320527483No Hit
ATACTGGGAATATTGGGACTGTTTCACAGCACAAACTCATCTTTACAGTGTT890.29940119760479045No Hit
CTAACAGGGTCATTCTCAGGGTACAGATGAGAACTCCAGCTCAGGATAATTA890.29940119760479045No Hit
GTAAATGGGTAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACTTAAAC890.29940119760479045No Hit
ACATATGGGGTCTATATTTATAAGATAAAATTACAAAAGTATTAGGCAAATT880.29603713920473657No Hit
TACGTTGGGGAGACTCCACCTCAAAAAATAAAATCAAGATAACCTCTTGAAT870.29267308080468274No Hit
AGTAGGGGGGTATCAGCCAACTGAGATGGCCGTCGTGCTCAACGGTGGGACC860.28930902240462897No Hit
AAGCACGGGCGCGCCCCGCCCGGGGTCCCCAGCCGATCGGGTCAGGCAAGTC840.2825809056045213No Hit
GCCACTGGGGCTACCCCTGTGTGGTCTCACTAGCCCCTCCAAGCTGGCAGTG840.2825809056045213No Hit
CATCATGGGGGCTCACTGCAACTCTGCCTCCCGGGTTCAAGCAATTCTCCTG840.2825809056045213No Hit
GCTTTAGGGTCTCCTGTCATCTTGAAATTCTAAATAATTTTTGAACAAAGTG840.2825809056045213No Hit
ATGCTAGGGGGGCAGCCTCTGCCGGGTTCCGGGAAAAGGAGCTCCTGCTGCC790.2657606136042522No Hit
GCCTACATTTTTGAGGAGCCCTTCACCATTCGAGACCTGCAGGCAAATGTTG790.2657606136042522No Hit
AACACAGGGTAAAGAGGATGAAATTACTGAAAAGAAAGCCAAAAAAGCCAAG770.25903249680414453No Hit
CGCGCTGGGATTGCAGGCGTGAGCCACTGCGCCCAGCCAGACGTCACTTTTT770.25903249680414453No Hit
TCTTGGGGAACTGCGATAATGATTGATTTTGCAAATTCCCTTTAAAAGCACA760.2556684384040907No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCACTTCCGATCTCGTATGCC760.2556684384040907No Hit
GCTCTCGGGTGTCCTTTTTTTTTAAGATTGTATTTTTACCATGGGCATTAAT730.24557626320392922No Hit
TGCTAAGGGGAGAAGAAGGGAAGGCGCGAGTGAGGAAAGGAGGTACTGTAGA730.24557626320392922No Hit
TTACAGGGCGCTCTTCCTATCTTGTTATGGGCCTCAGAAAAAACACCACATA720.2422122048038754No Hit
TAATAAGGGTATGGAAATTCTGCCAACAACTCAGCAGGAGGTAACTGTTCAC720.2422122048038754No Hit
AGGATAGGGAAGAGTAGCGGGCAGTGGGGGAGATGGTCTTACTCTGTTCCCC720.2422122048038754No Hit
GAAGCCGGGACCAATAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACT700.23548408800376772No Hit
CATACGGGAGTGTCACTTTTTTCCTGTCAATATATAGAGACTTCTAAATCAT700.23548408800376772No Hit
TACAACGGGACCCTTTAGACTGTAGCCTGGATATTATTCTTGTAGTTTACCT690.23212002960371392No Hit
CCAGCCTGGGCGACAGAGCGAGAGTCCATCTCAAAAACAAACAAACAAACAA680.2287559712036601No Hit
AGCAAGGGGTCTTGTAAATCTGGGGTCCATTATAAATAATAAATAAAGTTTT660.22202785440355247No Hit
ACGTTGGGTGGCAGGGATGGATGGCCCATGGCCCCTGGGAGCTGAGACCAGC650.21866379600349864No Hit
CTTATACACATCTCCGAGCCCACGAGACCACTTCCGATCCTGTCTCTTATAC640.21529973760344479No Hit
TTACAGCGGGGGGTGAGAGAAACCAAACCCTTCCCCCCATCACTTATGGTTC630.21193567920339096No Hit
TAACCGGGGAAGCTGACTGGTCCACGTACAGCCGAAGCATGAGGAAACTCAT630.21193567920339096No Hit
CTTCTGGGGACCAGGGAGGGCTCAGCCTGGATACCTCTGTGGTGAAGGCCTG630.21193567920339096No Hit
GACCAAGGGCTAATAAGCTGATATGTGATCACTAGTATTCTCATAAAACATC610.20520756240328333No Hit
GCATATGGGTCAGCCCGAAATGGGGGCTGTCACATATTAAGCTATCGACACA600.2018435040032295No Hit
AATAGAGGGTTATATTTTATGGGTGAAAGTTAGGAAGCAAAATAGCATATTA600.2018435040032295No Hit
TAATGGGGGGCTGATGGTCTGTATCTCATGTTCCCTAGTGTCTCTCTTTCAA600.2018435040032295No Hit
CATTTGGGTGCTTGAACCATAATCACATTTCTTTTGGTGAAGAAAAATAAAC600.2018435040032295No Hit
TTATAAGGGAATGTGTGTGGAAAGAGGTGTTCCTCATTATCTAATATTTCAA590.19847944560317568No Hit
AATTAAGGGATCAAGAGTCAAGTATGATGTTTCTTTAAGTTGACTTTTTTTA580.19511538720312185No Hit
CTTATAGGGTATTTTTTTAAATGTTTGTGGGCCAGTCTCATATAATTGATTA580.19511538720312185No Hit
GGTAATGGGATTGTAAAGGAAGAGTCAACTGGAAAATTCAAGGAGTTAATAA570.19175132880306803No Hit
CGCACGGGGGATCTCAGTAAGGGTCCCACGCCTGGGCTCTTGCCAGCAGTGG570.19175132880306803No Hit
TCAGAGGGTCTCACGTGAAATATAAAAAGCAAGTCTTGCCCAATAAAAACGC560.1883872704030142No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACTTCCGATCCTGTCTCT560.1883872704030142No Hit
CTTTGGGGGCGATCTAATGCCGGGCTCGTAGTCAGGCGGCGGCGCAGGCGGC560.1883872704030142No Hit
AAGTAGGGGTGTGACTCAGCAATCCTGTATCTACAATCCTTATTTTCATTAT560.1883872704030142No Hit
GCAGTTGGGAACCCTTCGCTGACGTGATTGGTTATGTGGCAGCAGAAGAAGC550.18502321200296037No Hit
AGGAGTGGGTATGTAGAAAATTGAAACTGGATCCCTTCCTTACATCATATAC550.18502321200296037No Hit
TTTGCCGGGTTCCCAGCAGGTTTTTGCCTTAGACGTGCTGGCCCCAGGACAG540.18165915360290655No Hit
TGTATTGGGTGACTACCTGGGGGAGCTGCTCTTAGAAGAAAAGAGATAAAGT540.18165915360290655No Hit
AAATGAGGGGTTTACAGGCCTGCTTTTTGCTTATAATTGACAACATGTGCAA540.18165915360290655No Hit
AAAACTGGGTGTAGGTAATTGTTAGATTTCGAGGATGAAGCTCTGAGACACT540.18165915360290655No Hit
TAACAAGGGATTCTTCATACCAAATACTAAATTATTTAGGTTCAATTTGCCG540.18165915360290655No Hit
ACTTCTGGGTCAGGCATTGAACAAGGGGACTTAGAAGACAGGGGTAATCCAA530.17829509520285272No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACTTCCGAACTCGTATGC520.1749310368027989No Hit
CACACTGGGTCTGCATGGGAATCAATGATTTCTCTACTTCAATGTAGTTTTC520.1749310368027989No Hit
CGAATTGGGTGCTAATTTGATTTGTGGTTTGGTAGGTAATATCTACACATTT520.1749310368027989No Hit
TTATACGGGGAAAAAGTAGAAGGAAAGAAGAAAGAAAAAGATGAAGAAAGAG520.1749310368027989No Hit
TTTTTGGGGTGTGTTTGGGATGCCTTTTCCTCAGATAGCCTCCTGGCTTTCT520.1749310368027989No Hit
TATCATGGGTTAAGTGTACTTCAAGTGCAGTATTTATGTAAAACCACTAAGA510.17156697840274507No Hit
AGCGCCGGGTCTCTGCTGAACTTTGAAAATAGCTTTTGGCTATGGTTAAATC510.17156697840274507No Hit
GGAAAGAGGGTTCATAAATACCTAGGCAAGAGGCAGGTCAACGGCACATGCG500.16820292000269124No Hit
TCGATCGGGTGGACATTTCCTATGGATGGAATTATGCACTATTTGGCCTTTT500.16820292000269124No Hit
ATACCTGGGTGACAGTGAGACTCGATCTCAGAAAAAAAAGAAATAGAAATCC500.16820292000269124No Hit
CACGCAGGGCTAATGTAGATGATGGGTTGATGAGTGCAGCAAATCACAATGG500.16820292000269124No Hit
TTAATGGGTAGAGGACGGGTCAGCTGTCTGGCTCAGTGATCTACATTCTGAA500.16820292000269124No Hit
ACCGCAGGGTGGGGATGAAAAGATCTTCAGTTAATGGAACAAGAACTAGAGG490.16483886160263742No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACTTCCGATCTCGT490.16483886160263742No Hit
ATGGGACGGTGAATGCATGAATGATTGAACGACAGAAAAGATTTGCATTGAC480.16147480320258362No Hit
GCTCAAGGGGTATTTCTCTGTTTCTATAGTGAATATTCTTATTTATCAAAAT480.16147480320258362No Hit
TCATAAGGGTGCAAGGACAATTGCAAGGTCCCATCTCCAAGGTGCGTCATAT480.16147480320258362No Hit
AGGCGGCAGGCACCATCAGGAGCAGGCTAGGGGGCAGGCCTGGGCCCAGCTC470.15811074480252976No Hit
AGCTGTGGGTGACTTGGGAATGGGGTAACTTCTTTATGCATAGTATTAAGGG470.15811074480252976No Hit
GATAACGGGTAGCCTCTTACCCAACAGTTTGCCTTTATATTCAAACTATTTG460.15474668640247594No Hit
AACAACGGGTGATCATGCCACTGCACTCTAGCCTGGGTGACAGAGCAAGTCC460.15474668640247594No Hit
AGTAACGGGTGCTAAGCAGATATATTCCAAAAATGGTAACTGTCATGTGCAC460.15474668640247594No Hit
TTAGGATGGGGACTGAGAAAAAAATGGATTAGAGTTTTAAAAAATCTAAATT460.15474668640247594No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACTTCCGACCTCGTATGC450.15138262800242214No Hit
TCCGTGGGGAAAATGGACTTAACATTCAGATGTTGACAATATAAGCTGCCTT440.14801856960236828No Hit
GGTGAAGGGTATTTTTCCTAGTGTGTCAGTAGATGATTAAAAATCATATTTT440.14801856960236828No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACTTCCGAT440.14801856960236828No Hit
ATGTATGGGTAGCTCAACCAACTCCTTTAGCATGTAAACTCACAGAAGGCAA440.14801856960236828No Hit
TTATTAGGGGTCCTATCAATGGAAACTTTAACAAAGGAGCTAGATTTTGGTT430.14465451120231448No Hit
GTATTAGGGTAAACAACCTAGCTTTACCCTTTCAGAAAGTGGCAAAAGAAAA430.14465451120231448No Hit
TCGTTAGGATTTCTAAGCTCAAGGTTAAAAATCATGTCACCTTTTTTTTTTT430.14465451120231448No Hit
AGCCTAGGGCACTGATGCTTGTCTGGTAATAATCACTTCTGTGTTATAATGG420.14129045280226066No Hit
CATCGGGGGTGGTTTGAATGCCTTTCTCCCCTGACTATCGAACAACCTGCTA420.14129045280226066No Hit
GATCAAGGGCCTCTCTTTTTCATGTCTCTTTTTTCTACAAATATTAAATCTT420.14129045280226066No Hit
CGGCTTCCTCACAGAGCCCTTTTTTCCTTCTGCCCAGCTCCATCCCACCAGA420.14129045280226066No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCACTTCCGATCTCGTATG420.14129045280226066No Hit
TCGTAGGGTAGTATTTTTATATGCCAACAGTGAATAATCTGTAAAAGAAATA410.1379263944022068No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACTTCCGATCTCGT410.1379263944022068No Hit
CTTGCTGGGTGGATATGTTAATTAGCTTGGTAGTAGTAATCATTTCATGATA410.1379263944022068No Hit
TTCAATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA410.1379263944022068No Hit
ATATGAGGGGCTACTTCAGAACATATTGTCTGGCACAAGATTTCTTCTAATA400.134562336002153No Hit
GTTAATGGGTGGCTAACCATTTTTGTGAGATAGTAAGTGTTAATGTTAATGA400.134562336002153No Hit
AAACTGGGCCGTGCTAGCCATTTGGGGGAAATATAGGTGAAGAGTGGCTGGG400.134562336002153No Hit
CTTCTTGGGTGTCCAAGACAGCCTGGCTGGGAAAGGGGGTGTGGAAAGAGCA400.134562336002153No Hit
TGTGCCTGGGAAGCTCACACCAAATTTTTCTATCAAATAAAACTAGTGACAG400.134562336002153No Hit
CGTCTGGGGTTAAGTATTTGAGGTGATGGATATGTTAATTGGCTTGATTTAA390.13119827760209918No Hit
TTAAACGGGCCGCTTCTCTCAGCTGGATATAAATACTATTTCTAAAAAATTA390.13119827760209918No Hit
AGCTGGGGGTACCAAGACTCTAGAATTTCACAACATTTAATAGGAAGAACTT390.13119827760209918No Hit
GTAGACGGGTAGAAATGAGATTTGGTGGAGTCTGGATTGCCTGTTTTGTATA380.12783421920204535No Hit
TAGACTGGGGTAGATCATGCAGCCACCATTGCTGTACACTAGAGTGGACTTT380.12783421920204535No Hit
CTTGGCGGGTGTTTCTACAAATTGTGGAATTGTTCTTATCTATAATGCTTTT380.12783421920204535No Hit
TCAGCAGGGTAACAAAGTTAGTTTTGAAAGGAAGCCCCACAGATCAAAAGGT380.12783421920204535No Hit
GTAGGAGGGAAGACGATGTGAATACATCTCCCCAAATCAATGAGAATTGTGC380.12783421920204535No Hit
ATCTGAGGGATCTGGCTAAGGGTGCCAAGGCTGGCAAATCAGTGTAATTTCA380.12783421920204535No Hit
CAAGCGGGTGACTGATCATGGCTCTTGCATTTTTGGGTAAATGGAGACTTCC370.12447016080199153No Hit
CTTATACACATCTCCGAGCCCACGAGACCACTTCCGACCTCGTATGCCGTCT370.12447016080199153No Hit
TAGCCGGGGAACTTTTTTTGAGCCTCAACTTTGCGTTGGTGTCAGTGTTCAG360.1211061024019377No Hit
TACATGGGGTAATAACACAAGAGACATCCCTTCACTTTTGTCACATTCTTCT360.1211061024019377No Hit
TAATAAGGGGAATTCCTCACAGATTAACCAGTATTTTTTAAATGTATTCATT350.11774204400188386No Hit
TTCCCTGGGTTATTTCACTGGGCCACAATTCAAACTTTACTACCTTCATTCT350.11774204400188386No Hit
TAGCAGGGGGTTTAAAAAGATGTTTATTCTGTTTGTCAGGTACAAAGATATT350.11774204400188386No Hit
TTGTTAGGGTAAAACAGAGAGGGTCTTTGCTTTACTGTTGTTTATAGTCTTG340.11437798560183005No Hit
TTTATGGGTACTCTTAAAATATTTAAGCAAGAAGTAATATAGGAAGGACTTA340.11437798560183005No Hit
CAACACGGGCTTATTGTTGAAACTTATTTTCAACAAAACTGTGCAGTTAAAT340.11437798560183005No Hit
ACTACCGGGAACCAAGTGGCAAGGTTGACTCCCAATGGGATGGCAGACTTTT330.11101392720177623No Hit
AGGCAGGAGGAGAGAGACAGAGGGGGAAGGAGAGAGACAAGTGCTGCTGTGG330.11101392720177623No Hit
TGATTAGGGTGGGGGAGAGGGTAGAGGTTTAAAGGTTTAAAACATGGATCTT330.11101392720177623No Hit
CGCAACGGGCATCTTGCTTCCGTTTCTCCAGGTTCATGGATCAACATCCGGA330.11101392720177623No Hit
TCTGTGGGGTATAAAATGGCATGTTCATATAGTGGAACACTATACAGCAGTT320.10764986880172239No Hit
CGTCCAGGGATGAGTTGAAAGATTTTGACTATTGAAAACCAAATTCTAGAAC320.10764986880172239No Hit
TGACGCGGGTGTGACTTAGAATAAAGGGATACAATTAGTTAATTTCAGGGCA320.10764986880172239No Hit
CTTTATGGGTCTGTATTTGCCACCTATATCATCATTTACAGTATGATAGTTG320.10764986880172239No Hit
GCCTTAGGGTACAGAAATGCATGTCATTAATTACGGACATGGTACTTTAAAT320.10764986880172239No Hit
TTGTGTCGGGGTTATATGTTTCATAGAATGATACTGCTTTTCACTCTTGATG320.10764986880172239No Hit
AGTACGGGGTGTGCTTGTTCAAACAAGGACTGATCTACAGAACACTTTAGTC320.10764986880172239No Hit
AACAACGGGGTCATGCACACCCCTGAACCACGAGGAAACAGTACAGTCGCTA320.10764986880172239No Hit
GCGGCGGGGATTTAAACAAAATAATATTTTTAGAAAGTGGTATCAAATATGA320.10764986880172239No Hit
CATTATGGGTATGCCAAAACAAGTTTCTATAACACCCCAGAAAGACCACAGC320.10764986880172239No Hit
TATTGGGGGATCTTGCCATGGGCGATCTTTTGCTGGGCGGCTCACTGAAGCC310.10428581040166857No Hit
CTGCACGGGTACTGAATCTTCTACGACTGAATTAGAAGAATAATGACTGTAC310.10428581040166857No Hit
GTTACAGGGTGGTATTTTGATGGGAATTGCATTGAATCTATAGATTGCTTTT310.10428581040166857No Hit
GCGTATGGGAATGTCTGGTGTTTGTCATGGTGAAATTATAACTATTACAGTG310.10428581040166857No Hit
TTCTTAGGGTCCCCTCTTGTGGCTTTTTGCTTTGTTTCCTTTCATTCACCCT310.10428581040166857No Hit
TTCGAAGGGGTCATCTGGAGCAGGGCAGGTAACCACAGGAGGAGGCATGCCC310.10428581040166857No Hit
ATTATAGGGTAGTCACCATGGTTTGCTCTCAGAAAACACATGTGACATGGCC300.10092175200161475No Hit
TGTAGCGGGTATTGTAGATTATTACAAACTTACAAAGGGCAACATTTTATTA300.10092175200161475No Hit
CTTAACGGGTAAAGAGGTATATTGGGACAATTAGTCATCTTTCCTACATTTT300.10092175200161475No Hit
TGTCCTGGGTGTTAGGTCACAAGACAAGTCTTAAAACATTCAAAAAATTGAA300.10092175200161475No Hit
TGCCGTTGGGTAAAAACAAAAACTTGTTACTAAAGAAAATATCTGAAATGTG300.10092175200161475No Hit
TGGCTACGGGTAAATCAGCAATTCAAGGGGAGGGTAAAAAGACCAACTAATA300.10092175200161475No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)