FastQCFastQC Report
Sat 31 Dec 2022
SRR5846547.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846547.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29286
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACTTCCGATCTCGTATGC7502.5609506248719525No Hit
CTTATACACATCTCCGAGCCCACGAGACCACTTCCGATCTCGTATGCCGTCT6732.298026360718432No Hit
CTTATACACATCTCCGAGCCCACGAGACCACTTCCGACTGTCTCTTATACAC1490.508775524141228No Hit
ATATGTGGGCATGCTGATTGAGTGTTTTCCTGTACCATAGTAACTACATTGA1400.47804411664276447No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACTTCCG1360.4643857133101141No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACTTCCGACTGTCTCTTA1350.4609711124769515No Hit
ATTGGCGGGATGCTTTTTCTTGATGCTAAGATCACTGCAGAAGAATAACCTG1280.43706890664481324No Hit
AGTCTCGGGATTCTCTCTTCAGGTATTCCTCCTTTGGTGTGGTGTCATTGAG1270.43365430581165065No Hit
ATTCTGGGATTTGATGCTCTCTTGTATTTTAAATCTCATTTAAATCTTTGTC1250.4268251041453254No Hit
ATGAAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1200.4097520999795124No Hit
AAGTGCGGGCTTTGGAGGGGGTGGGGTCCGTTGGCATCAACACGGGGACCCT1110.37902069248104897No Hit
GCTCAAGGGGGGAGCAGGCGCTGCGGCAGGAGGGAAGATGGCGGACGAGGAG1100.3756060916478864No Hit
ACGCTTGGGCTGAGCGCGAGGCTGCAAATTTCATCATGAAGGTCATCCAGAG1060.36194768831523594No Hit
AAATAAGGGTCCCAGAAGGACCTCCTGAGCGATGACTCAAGTGTCTCAGTCC1060.36194768831523594No Hit
CACCAAGGGGGGAGTTGGGAGCAAAGCGGAGGCCCGGGCTGCCCGCCGTCCC1030.35170388581574813No Hit
GTAAATGGGTAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACTTAAAC1010.3448746841494229No Hit
AAGCACGGGCGCGCCCCGCCCGGGGTCCCCAGCCGATCGGGTCAGGCAAGTC990.33804548248309774No Hit
ATGCTAGGGGGGCAGCCTCTGCCGGGTTCCGGGAAAAGGAGCTCCTGCTGCC990.33804548248309774No Hit
TACGTTGGGGAGACTCCACCTCAAAAAATAAAATCAAGATAACCTCTTGAAT980.33463088164993515No Hit
GCCCAGCAGCAGAGCTCATGACAAACAGTAAACCCCAGCGAAAGGACCTGGC980.33463088164993515No Hit
ATACTGGGAATATTGGGACTGTTTCACAGCACAAACTCATCTTTACAGTGTT960.32780167998360993No Hit
ACATATGGGGTCTATATTTATAAGATAAAATTACAAAAGTATTAGGCAAATT930.3175578774841221No Hit
CGCGCTGGGATTGCAGGCGTGAGCCACTGCGCCCAGCCAGACGTCACTTTTT880.3004848733183091No Hit
TGCTAAGGGGAGAAGAAGGGAAGGCGCGAGTGAGGAAAGGAGGTACTGTAGA880.3004848733183091No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCACTTCCGA870.2970702724851465No Hit
TAACAAGGGATTCTTCATACCAAATACTAAATTATTTAGGTTCAATTTGCCG870.2970702724851465No Hit
AGTAGGGGGGTATCAGCCAACTGAGATGGCCGTCGTGCTCAACGGTGGGACC860.2936556716519839No Hit
GCTTTAGGGTCTCCTGTCATCTTGAAATTCTAAATAATTTTTGAACAAAGTG820.27999726831933347No Hit
GCTCTCGGGTGTCCTTTTTTTTTAAGATTGTATTTTTACCATGGGCATTAAT810.27658266748617083No Hit
TTACAGGGCGCTCTTCCTATCTTGTTATGGGCCTCAGAAAAAACACCACATA800.27316806665300825No Hit
GGAAAGAGGGTTCATAAATACCTAGGCAAGAGGCAGGTCAACGGCACATGCG780.2663388649866831No Hit
GCATATGGGTCAGCCCGAAATGGGGGCTGTCACATATTAAGCTATCGACACA760.25950966332035785No Hit
AACACAGGGTAAAGAGGATGAAATTACTGAAAAGAAAGCCAAAAAAGCCAAG760.25950966332035785No Hit
CCAGCCTGGGCGACAGAGCGAGAGTCCATCTCAAAAACAAACAAACAAACAA750.25609506248719527No Hit
AGGATAGGGAAGAGTAGCGGGCAGTGGGGGAGATGGTCTTACTCTGTTCCCC740.25268046165403263No Hit
AATTAAGGGATCAAGAGTCAAGTATGATGTTTCTTTAAGTTGACTTTTTTTA730.24926586082087004No Hit
ATAGTTGGGTCCGATCTTGCTGTGGGTCTGTGAAAGGCACTTTAATTATCAC720.2458512599877074No Hit
TCTTGGGGAACTGCGATAATGATTGATTTTGCAAATTCCCTTTAAAAGCACA700.23902205832138224No Hit
AGGCGGCAGGCACCATCAGGAGCAGGCTAGGGGGCAGGCCTGGGCCCAGCTC690.23560745748821962No Hit
ACGTTGGGTGGCAGGGATGGATGGCCCATGGCCCCTGGGAGCTGAGACCAGC690.23560745748821962No Hit
AATAGAGGGTTATATTTTATGGGTGAAAGTTAGGAAGCAAAATAGCATATTA690.23560745748821962No Hit
TACAACGGGACCCTTTAGACTGTAGCCTGGATATTATTCTTGTAGTTTACCT680.23219285665505704No Hit
TCAGAGGGTCTCACGTGAAATATAAAAAGCAAGTCTTGCCCAATAAAAACGC680.23219285665505704No Hit
CTAACAGGGTCATTCTCAGGGTACAGATGAGAACTCCAGCTCAGGATAATTA680.23219285665505704No Hit
AGGAGTGGGTATGTAGAAAATTGAAACTGGATCCCTTCCTTACATCATATAC680.23219285665505704No Hit
GCCTACATTTTTGAGGAGCCCTTCACCATTCGAGACCTGCAGGCAAATGTTG670.2287782558218944No Hit
GACCAAGGGCTAATAAGCTGATATGTGATCACTAGTATTCTCATAAAACATC660.22536365498873182No Hit
CATCATGGGGGCTCACTGCAACTCTGCCTCCCGGGTTCAAGCAATTCTCCTG650.2219490541555692No Hit
TAACCGGGGAAGCTGACTGGTCCACGTACAGCCGAAGCATGAGGAAACTCAT650.2219490541555692No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCACTTCCGATCTCGTATGCC650.2219490541555692No Hit
TAATAAGGGTATGGAAATTCTGCCAACAACTCAGCAGGAGGTAACTGTTCAC640.21853445332240662No Hit
CACGCAGGGCTAATGTAGATGATGGGTTGATGAGTGCAGCAAATCACAATGG640.21853445332240662No Hit
CGAATTGGGTGCTAATTTGATTTGTGGTTTGGTAGGTAATATCTACACATTT630.21511985248924403No Hit
TTATAAGGGAATGTGTGTGGAAAGAGGTGTTCCTCATTATCTAATATTTCAA630.21511985248924403No Hit
GAAGCCGGGACCAATAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACT620.2117052516560814No Hit
CATTTGGGTGCTTGAACCATAATCACATTTCTTTTGGTGAAGAAAAATAAAC620.2117052516560814No Hit
TAATGGGGGGCTGATGGTCTGTATCTCATGTTCCCTAGTGTCTCTCTTTCAA610.2082906508229188No Hit
CTTTGGGGGCGATCTAATGCCGGGCTCGTAGTCAGGCGGCGGCGCAGGCGGC600.2048760499897562No Hit
CATACGGGAGTGTCACTTTTTTCCTGTCAATATATAGAGACTTCTAAATCAT590.2014614491565936No Hit
ACTTCTGGGTCAGGCATTGAACAAGGGGACTTAGAAGACAGGGGTAATCCAA580.19804684832343097No Hit
AGCAAGGGGTCTTGTAAATCTGGGGTCCATTATAAATAATAAATAAAGTTTT580.19804684832343097No Hit
CGGCTTCCTCACAGAGCCCTTTTTTCCTTCTGCCCAGCTCCATCCCACCAGA570.1946322474902684No Hit
ATGGGACGGTGAATGCATGAATGATTGAACGACAGAAAAGATTTGCATTGAC560.19121764665710578No Hit
GCCACTGGGGCTACCCCTGTGTGGTCTCACTAGCCCCTCCAAGCTGGCAGTG560.19121764665710578No Hit
GGTAATGGGATTGTAAAGGAAGAGTCAACTGGAAAATTCAAGGAGTTAATAA560.19121764665710578No Hit
CACACTGGGTCTGCATGGGAATCAATGATTTCTCTACTTCAATGTAGTTTTC550.1878030458239432No Hit
CTTATACACATCTCCGAGCCCACGAGACCACTTCCGATCCTGTCTCTTATAC550.1878030458239432No Hit
TTTGCCGGGTTCCCAGCAGGTTTTTGCCTTAGACGTGCTGGCCCCAGGACAG540.18438844499078058No Hit
ATACCTGGGTGACAGTGAGACTCGATCTCAGAAAAAAAAGAAATAGAAATCC540.18438844499078058No Hit
AAAACTGGGTGTAGGTAATTGTTAGATTTCGAGGATGAAGCTCTGAGACACT530.18097384415761797No Hit
AGCGCCGGGTCTCTGCTGAACTTTGAAAATAGCTTTTGGCTATGGTTAAATC530.18097384415761797No Hit
GCAGTTGGGAACCCTTCGCTGACGTGATTGGTTATGTGGCAGCAGAAGAAGC520.17755924332445536No Hit
GGTGAAGGGTATTTTTCCTAGTGTGTCAGTAGATGATTAAAAATCATATTTT520.17755924332445536No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACTTCCGAACTCGTATGC510.17414464249129277No Hit
GTAGACGGGTAGAAATGAGATTTGGTGGAGTCTGGATTGCCTGTTTTGTATA500.17073004165813016No Hit
CTTATAGGGTATTTTTTTAAATGTTTGTGGGCCAGTCTCATATAATTGATTA490.16731544082496758No Hit
TCATAAGGGTGCAAGGACAATTGCAAGGTCCCATCTCCAAGGTGCGTCATAT490.16731544082496758No Hit
CGCACGGGGGATCTCAGTAAGGGTCCCACGCCTGGGCTCTTGCCAGCAGTGG490.16731544082496758No Hit
AACAACGGGTGATCATGCCACTGCACTCTAGCCTGGGTGACAGAGCAAGTCC480.16390083999180496No Hit
AGTAACGGGTGCTAAGCAGATATATTCCAAAAATGGTAACTGTCATGTGCAC480.16390083999180496No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACTTCCGAT480.16390083999180496No Hit
TTATTAGGGGTCCTATCAATGGAAACTTTAACAAAGGAGCTAGATTTTGGTT470.16048623915864235No Hit
AACAACGGGGTCATGCACACCCCTGAACCACGAGGAAACAGTACAGTCGCTA470.16048623915864235No Hit
GCTCAAGGGGTATTTCTCTGTTTCTATAGTGAATATTCTTATTTATCAAAAT460.15707163832547974No Hit
TATTGGGGGATCTTGCCATGGGCGATCTTTTGCTGGGCGGCTCACTGAAGCC440.15024243665915454No Hit
GTTAATGGGTGGCTAACCATTTTTGTGAGATAGTAAGTGTTAATGTTAATGA440.15024243665915454No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACTTCCGATCTCGT440.15024243665915454No Hit
TCGATCGGGTGGACATTTCCTATGGATGGAATTATGCACTATTTGGCCTTTT430.14682783582599196No Hit
TTGCTGGGATTACAGGCCTGAGCCACTGTGCCCAGCCAAAATGTGCCTTTGC430.14682783582599196No Hit
TAGACTGGGGTAGATCATGCAGCCACCATTGCTGTACACTAGAGTGGACTTT430.14682783582599196No Hit
AGCCTAGGGCACTGATGCTTGTCTGGTAATAATCACTTCTGTGTTATAATGG420.14341323499282935No Hit
GTATTAGGGTAAACAACCTAGCTTTACCCTTTCAGAAAGTGGCAAAAGAAAA420.14341323499282935No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACTTCCGATCTCGT420.14341323499282935No Hit
CTTGCTGGGTGGATATGTTAATTAGCTTGGTAGTAGTAATCATTTCATGATA420.14341323499282935No Hit
TTCAATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA420.14341323499282935No Hit
CATCTGGGTAGTGATGATCTCTGGTTTGTAGGAATGTACCATTTTATTTCCT420.14341323499282935No Hit
TTGTGTCGGGGTTATATGTTTCATAGAATGATACTGCTTTTCACTCTTGATG420.14341323499282935No Hit
TTACAGCGGGGGGTGAGAGAAACCAAACCCTTCCCCCCATCACTTATGGTTC420.14341323499282935No Hit
TCGTTAGGATTTCTAAGCTCAAGGTTAAAAATCATGTCACCTTTTTTTTTTT410.13999863415966673No Hit
AGCTGGGGGTACCAAGACTCTAGAATTTCACAACATTTAATAGGAAGAACTT410.13999863415966673No Hit
TTCCCTGGGTTATTTCACTGGGCCACAATTCAAACTTTACTACCTTCATTCT410.13999863415966673No Hit
TTAATGGGTAGAGGACGGGTCAGCTGTCTGGCTCAGTGATCTACATTCTGAA410.13999863415966673No Hit
TTTGTGGGGGGGGGCAGGATCTGGCCCGAGCTCTCCTCAGACCTCCCCAGAC410.13999863415966673No Hit
CTTCTGGGGACCAGGGAGGGCTCAGCCTGGATACCTCTGTGGTGAAGGCCTG410.13999863415966673No Hit
TGTAGCGGGTATTGTAGATTATTACAAACTTACAAAGGGCAACATTTTATTA400.13658403332650412No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACTTCCGATCCTGTCTCT400.13658403332650412No Hit
CTTGGCGGGTGTTTCTACAAATTGTGGAATTGTTCTTATCTATAATGCTTTT400.13658403332650412No Hit
CTTCTTGGGTGTCCAAGACAGCCTGGCTGGGAAAGGGGGTGTGGAAAGAGCA400.13658403332650412No Hit
TTAGGATGGGGACTGAGAAAAAAATGGATTAGAGTTTTAAAAAATCTAAATT400.13658403332650412No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCACTTCCGATCTCGTATG400.13658403332650412No Hit
TTATACGGGGAAAAAGTAGAAGGAAAGAAGAAAGAAAAAGATGAAGAAAGAG400.13658403332650412No Hit
AGGCAGGAGGAGAGAGACAGAGGGGGAAGGAGAGAGACAAGTGCTGCTGTGG390.13316943249334154No Hit
CTTAACGGGTAAAGAGGTATATTGGGACAATTAGTCATCTTTCCTACATTTT380.12975483166017893No Hit
TCAGCAGGGTAACAAAGTTAGTTTTGAAAGGAAGCCCCACAGATCAAAAGGT380.12975483166017893No Hit
GGACACGGGATTTGTTATTTCAGATCTTCCCAAAAAGAAAACTCTGAGCAAA380.12975483166017893No Hit
CAAGCGGGTGACTGATCATGGCTCTTGCATTTTTGGGTAAATGGAGACTTCC380.12975483166017893No Hit
TATCATGGGTTAAGTGTACTTCAAGTGCAGTATTTATGTAAAACCACTAAGA370.12634023082701631No Hit
CATCGGGGGTGGTTTGAATGCCTTTCTCCCCTGACTATCGAACAACCTGCTA370.12634023082701631No Hit
GCACACGGGATTAAAATACTAAAAGCAAAGATGTTGTGTGTGTACTGACCCA370.12634023082701631No Hit
ACCGCAGGGTGGGGATGAAAAGATCTTCAGTTAATGGAACAAGAACTAGAGG370.12634023082701631No Hit
TTAAACGGGCCGCTTCTCTCAGCTGGATATAAATACTATTTCTAAAAAATTA370.12634023082701631No Hit
CAGCACTGGTCAAGAAAACAAGATGGCAACATATATGCTTTCAGGGTCAAAT360.1229256299938537No Hit
TCGTAGGGTAGTATTTTTATATGCCAACAGTGAATAATCTGTAAAAGAAATA360.1229256299938537No Hit
CTTTATGGGTCTGTATTTGCCACCTATATCATCATTTACAGTATGATAGTTG360.1229256299938537No Hit
AAGTAGGGGTGTGACTCAGCAATCCTGTATCTACAATCCTTATTTTCATTAT360.1229256299938537No Hit
AGCTGTGGGTGACTTGGGAATGGGGTAACTTCTTTATGCATAGTATTAAGGG350.11951102916069112No Hit
CAACACGGGCTTATTGTTGAAACTTATTTTCAACAAAACTGTGCAGTTAAAT350.11951102916069112No Hit
AGTACGGGGTGTGCTTGTTCAAACAAGGACTGATCTACAGAACACTTTAGTC350.11951102916069112No Hit
GCGGCGGGGATTTAAACAAAATAATATTTTTAGAAAGTGGTATCAAATATGA350.11951102916069112No Hit
ATATACGGGTAGGGAAGTCCAAAGTCAAGAGCAGAAACAAAAGACACTAGAG350.11951102916069112No Hit
CGCAACGGGCATCTTGCTTCCGTTTCTCCAGGTTCATGGATCAACATCCGGA350.11951102916069112No Hit
GTGTGCGGGTAGCGGGTGCCTGTAATCCCAGCTACTTGGGAGGCTGAGAGAG340.11609642832752852No Hit
CGTCCAGGGATGAGTTGAAAGATTTTGACTATTGAAAACCAAATTCTAGAAC340.11609642832752852No Hit
GATCAAGGGCCTCTCTTTTTCATGTCTCTTTTTTCTACAAATATTAAATCTT340.11609642832752852No Hit
TGTATTGGGTGACTACCTGGGGGAGCTGCTCTTAGAAGAAAAGAGATAAAGT340.11609642832752852No Hit
TTGTTAGGGTAAAACAGAGAGGGTCTTTGCTTTACTGTTGTTTATAGTCTTG340.11609642832752852No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACTTCCGACCTCGTATGC340.11609642832752852No Hit
GCATTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG340.11609642832752852No Hit
ACTACCGGGAACCAAGTGGCAAGGTTGACTCCCAATGGGATGGCAGACTTTT330.11268182749436591No Hit
TAGCCGGGGAACTTTTTTTGAGCCTCAACTTTGCGTTGGTGTCAGTGTTCAG330.11268182749436591No Hit
ATCTGAGGGATCTGGCTAAGGGTGCCAAGGCTGGCAAATCAGTGTAATTTCA330.11268182749436591No Hit
ATTATAGGGTAGTCACCATGGTTTGCTCTCAGAAAACACATGTGACATGGCC320.10926722666120331No Hit
TGGCCTGGGACCTGTAATCCCACTACTTTGGTTGGCCGAGGCGGGAGCGTAT320.10926722666120331No Hit
TTCTTAGGGTCCCCTCTTGTGGCTTTTTGCTTTGTTTCCTTTCATTCACCCT320.10926722666120331No Hit
TAGCAGGGGGTTTAAAAAGATGTTTATTCTGTTTGTCAGGTACAAAGATATT320.10926722666120331No Hit
CCTGCAGGGTGATGTGTGTGGATGAGCTAGGAAGACCTGAAATAGGCTCTCT320.10926722666120331No Hit
TAATAAGGGGAATTCCTCACAGATTAACCAGTATTTTTTAAATGTATTCATT310.1058526258280407No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCA310.1058526258280407No Hit
GATAACGGGTAGCCTCTTACCCAACAGTTTGCCTTTATATTCAAACTATTTG310.1058526258280407No Hit
CCAAATGGGTCTGACCACGGGTCTCATCAGTTTTATTGCCTGCTCGATTATA310.1058526258280407No Hit
AAAATAGGGGTTCTGCACAGGTTTCTAGACCTTATGGAAGGAAACTCTGTGT310.1058526258280407No Hit
ATAGTGGGGGAATTGAGAAGTGCTCATGAAAATGCATAGGTAAATTATAAAA310.1058526258280407No Hit
AAATGAGGGGTTTACAGGCCTGCTTTTTGCTTATAATTGACAACATGTGCAA310.1058526258280407No Hit
TATCCAGGGTGGTTTCATCATGTTGGTCAGGCTGGTCTCGGACTCCTGACCT300.1024380249948781No Hit
TGTCCTGGGTGTTAGGTCACAAGACAAGTCTTAAAACATTCAAAAAATTGAA300.1024380249948781No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)