FastQCFastQC Report
Sat 31 Dec 2022
SRR5846546.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846546.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28883
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACTTCCGATCTCGTATGC7742.6797770314718No Hit
CTTATACACATCTCCGAGCCCACGAGACCACTTCCGATCTCGTATGCCGTCT6892.385486272201641No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACTTCCG1520.5262611224595783No Hit
CTTATACACATCTCCGAGCCCACGAGACCACTTCCGACTGTCTCTTATACAC1460.5054876570993317No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACTTCCGACTGTCTCTTA1390.4812519475123775No Hit
ATTCTGGGATTTGATGCTCTCTTGTATTTTAAATCTCATTTAAATCTTTGTC1240.42931828411176126No Hit
ATTGGCGGGATGCTTTTTCTTGATGCTAAGATCACTGCAGAAGAATAACCTG1220.4223937956583457No Hit
ATATGTGGGCATGCTGATTGAGTGTTTTCCTGTACCATAGTAACTACATTGA1170.405082574524807No Hit
TGCTAAGGGGAGAAGAAGGGAAGGCGCGAGTGAGGAAAGGAGGTACTGTAGA1110.38430910916456046No Hit
AAGCACGGGCGCGCCCCGCCCGGGGTCCCCAGCCGATCGGGTCAGGCAAGTC1060.3669978880310217No Hit
ATGAAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1030.35661115535089843No Hit
ACATATGGGGTCTATATTTATAAGATAAAATTACAAAAGTATTAGGCAAATT1020.35314891112419067No Hit
AAGTGCGGGCTTTGGAGGGGGTGGGGTCCGTTGGCATCAACACGGGGACCCT1020.35314891112419067No Hit
AAATAAGGGTCCCAGAAGGACCTCCTGAGCGATGACTCAAGTGTCTCAGTCC990.3427621784440674No Hit
AGTCTCGGGATTCTCTCTTCAGGTATTCCTCCTTTGGTGTGGTGTCATTGAG980.3392999342173597No Hit
GCCCAGCAGCAGAGCTCATGACAAACAGTAAACCCCAGCGAAAGGACCTGGC930.32198871308382093No Hit
GCTCAAGGGGGGAGCAGGCGCTGCGGCAGGAGGGAAGATGGCGGACGAGGAG930.32198871308382093No Hit
ATAGTTGGGTCCGATCTTGCTGTGGGTCTGTGAAAGGCACTTTAATTATCAC910.31506422463040545No Hit
AGGCGGCAGGCACCATCAGGAGCAGGCTAGGGGGCAGGCCTGGGCCCAGCTC890.3081397361769899No Hit
ACGCTTGGGCTGAGCGCGAGGCTGCAAATTTCATCATGAAGGTCATCCAGAG890.3081397361769899No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCACTTCCGA890.3081397361769899No Hit
CATACGGGAGTGTCACTTTTTTCCTGTCAATATATAGAGACTTCTAAATCAT890.3081397361769899No Hit
TACGTTGGGGAGACTCCACCTCAAAAAATAAAATCAAGATAACCTCTTGAAT870.3012152477235744No Hit
TAACAAGGGATTCTTCATACCAAATACTAAATTATTTAGGTTCAATTTGCCG870.3012152477235744No Hit
ATACTGGGAATATTGGGACTGTTTCACAGCACAAACTCATCTTTACAGTGTT860.2977530034968667No Hit
GTAAATGGGTAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACTTAAAC860.2977530034968667No Hit
CATTTGGGTGCTTGAACCATAATCACATTTCTTTTGGTGAAGAAAAATAAAC800.27697953813662013No Hit
CACCAAGGGGGGAGTTGGGAGCAAAGCGGAGGCCCGGGCTGCCCGCCGTCCC800.27697953813662013No Hit
TACAACGGGACCCTTTAGACTGTAGCCTGGATATTATTCTTGTAGTTTACCT790.2735172939099124No Hit
GCTTTAGGGTCTCCTGTCATCTTGAAATTCTAAATAATTTTTGAACAAAGTG790.2735172939099124No Hit
GCTCTCGGGTGTCCTTTTTTTTTAAGATTGTATTTTTACCATGGGCATTAAT760.2631305612297892No Hit
AGGATAGGGAAGAGTAGCGGGCAGTGGGGGAGATGGTCTTACTCTGTTCCCC760.2631305612297892No Hit
ATGCTAGGGGGGCAGCCTCTGCCGGGTTCCGGGAAAAGGAGCTCCTGCTGCC740.25620607277637364No Hit
AATAGAGGGTTATATTTTATGGGTGAAAGTTAGGAAGCAAAATAGCATATTA740.25620607277637364No Hit
AACACAGGGTAAAGAGGATGAAATTACTGAAAAGAAAGCCAAAAAAGCCAAG730.25274382854966587No Hit
TAATAAGGGTATGGAAATTCTGCCAACAACTCAGCAGGAGGTAACTGTTCAC730.25274382854966587No Hit
TTACAGGGCGCTCTTCCTATCTTGTTATGGGCCTCAGAAAAAACACCACATA720.24928158432295813No Hit
AGTAGGGGGGTATCAGCCAACTGAGATGGCCGTCGTGCTCAACGGTGGGACC720.24928158432295813No Hit
GAAGCCGGGACCAATAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACT710.24581934009625042No Hit
GCCTACATTTTTGAGGAGCCCTTCACCATTCGAGACCTGCAGGCAAATGTTG700.24235709586954263No Hit
TCGATCGGGTGGACATTTCCTATGGATGGAATTATGCACTATTTGGCCTTTT690.2388948516428349No Hit
TCTTGGGGAACTGCGATAATGATTGATTTTGCAAATTCCCTTTAAAAGCACA690.2388948516428349No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCACTTCCGATCTCGTATGCC690.2388948516428349No Hit
ACTTCTGGGTCAGGCATTGAACAAGGGGACTTAGAAGACAGGGGTAATCCAA670.23197036318941938No Hit
TAATGGGGGGCTGATGGTCTGTATCTCATGTTCCCTAGTGTCTCTCTTTCAA670.23197036318941938No Hit
TTAATGGGTAGAGGACGGGTCAGCTGTCTGGCTCAGTGATCTACATTCTGAA650.22504587473600388No Hit
CTTATAGGGTATTTTTTTAAATGTTTGTGGGCCAGTCTCATATAATTGATTA630.21812138628258837No Hit
CATCATGGGGGCTCACTGCAACTCTGCCTCCCGGGTTCAAGCAATTCTCCTG630.21812138628258837No Hit
AGTACGGGGTGTGCTTGTTCAAACAAGGACTGATCTACAGAACACTTTAGTC630.21812138628258837No Hit
AAAACTGGGTGTAGGTAATTGTTAGATTTCGAGGATGAAGCTCTGAGACACT630.21812138628258837No Hit
AGCGCCGGGTCTCTGCTGAACTTTGAAAATAGCTTTTGGCTATGGTTAAATC630.21812138628258837No Hit
GGTAATGGGATTGTAAAGGAAGAGTCAACTGGAAAATTCAAGGAGTTAATAA610.21119689782917286No Hit
GCATATGGGTCAGCCCGAAATGGGGGCTGTCACATATTAAGCTATCGACACA600.20773465360246512No Hit
GCCACTGGGGCTACCCCTGTGTGGTCTCACTAGCCCCTCCAAGCTGGCAGTG600.20773465360246512No Hit
AGTAACGGGTGCTAAGCAGATATATTCCAAAAATGGTAACTGTCATGTGCAC600.20773465360246512No Hit
CACACTGGGTCTGCATGGGAATCAATGATTTCTCTACTTCAATGTAGTTTTC590.20427240937575739No Hit
CCAGCCTGGGCGACAGAGCGAGAGTCCATCTCAAAAACAAACAAACAAACAA590.20427240937575739No Hit
GACCAAGGGCTAATAAGCTGATATGTGATCACTAGTATTCTCATAAAACATC590.20427240937575739No Hit
CGGCTTCCTCACAGAGCCCTTTTTTCCTTCTGCCCAGCTCCATCCCACCAGA580.20081016514904962No Hit
GCAGTTGGGAACCCTTCGCTGACGTGATTGGTTATGTGGCAGCAGAAGAAGC570.19734792092234188No Hit
TTTGCCGGGTTCCCAGCAGGTTTTTGCCTTAGACGTGCTGGCCCCAGGACAG570.19734792092234188No Hit
GGAAAGAGGGTTCATAAATACCTAGGCAAGAGGCAGGTCAACGGCACATGCG560.1938856766956341No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACTTCCGAACTCGTATGC560.1938856766956341No Hit
TAACCGGGGAAGCTGACTGGTCCACGTACAGCCGAAGCATGAGGAAACTCAT560.1938856766956341No Hit
TCAGAGGGTCTCACGTGAAATATAAAAAGCAAGTCTTGCCCAATAAAAACGC550.19042343246892637No Hit
CGCACGGGGGATCTCAGTAAGGGTCCCACGCCTGGGCTCTTGCCAGCAGTGG550.19042343246892637No Hit
AGGAGTGGGTATGTAGAAAATTGAAACTGGATCCCTTCCTTACATCATATAC540.1869611882422186No Hit
TTAGGATGGGGACTGAGAAAAAAATGGATTAGAGTTTTAAAAAATCTAAATT540.1869611882422186No Hit
CGCGCTGGGATTGCAGGCGTGAGCCACTGCGCCCAGCCAGACGTCACTTTTT530.18349894401551084No Hit
AGCAAGGGGTCTTGTAAATCTGGGGTCCATTATAAATAATAAATAAAGTTTT530.18349894401551084No Hit
ATACCTGGGTGACAGTGAGACTCGATCTCAGAAAAAAAAGAAATAGAAATCC520.1800366997888031No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACTTCCGATCTCGT520.1800366997888031No Hit
TTATAAGGGAATGTGTGTGGAAAGAGGTGTTCCTCATTATCTAATATTTCAA520.1800366997888031No Hit
AAATGAGGGGTTTACAGGCCTGCTTTTTGCTTATAATTGACAACATGTGCAA520.1800366997888031No Hit
AGCCTAGGGCACTGATGCTTGTCTGGTAATAATCACTTCTGTGTTATAATGG510.17657445556209533No Hit
AATTAAGGGATCAAGAGTCAAGTATGATGTTTCTTTAAGTTGACTTTTTTTA510.17657445556209533No Hit
GGTGAAGGGTATTTTTCCTAGTGTGTCAGTAGATGATTAAAAATCATATTTT510.17657445556209533No Hit
CTTATACACATCTCCGAGCCCACGAGACCACTTCCGATCCTGTCTCTTATAC510.17657445556209533No Hit
CTTTGGGGGCGATCTAATGCCGGGCTCGTAGTCAGGCGGCGGCGCAGGCGGC490.16964996710867986No Hit
TCAGCAGGGTAACAAAGTTAGTTTTGAAAGGAAGCCCCACAGATCAAAAGGT490.16964996710867986No Hit
TTCTTAGGGTCCCCTCTTGTGGCTTTTTGCTTTGTTTCCTTTCATTCACCCT490.16964996710867986No Hit
GCGGCGGGGATTTAAACAAAATAATATTTTTAGAAAGTGGTATCAAATATGA480.1661877228819721No Hit
ACGTTGGGTGGCAGGGATGGATGGCCCATGGCCCCTGGGAGCTGAGACCAGC470.16272547865526435No Hit
GTAGGAGGGAAGACGATGTGAATACATCTCCCCAAATCAATGAGAATTGTGC470.16272547865526435No Hit
CTTCTGGGGACCAGGGAGGGCTCAGCCTGGATACCTCTGTGGTGAAGGCCTG470.16272547865526435No Hit
CTAACAGGGTCATTCTCAGGGTACAGATGAGAACTCCAGCTCAGGATAATTA460.15926323442855658No Hit
ATATGAGGGGCTACTTCAGAACATATTGTCTGGCACAAGATTTCTTCTAATA450.15580099020184882No Hit
CTTTATGGGTCTGTATTTGCCACCTATATCATCATTTACAGTATGATAGTTG450.15580099020184882No Hit
CATATAGGGGTCCTTTATAAGCTTTGAGTATCTTACAGCTTGCCTTTTTAAT440.1523387459751411No Hit
TTATACGGGGAAAAAGTAGAAGGAAAGAAGAAAGAAAAAGATGAAGAAAGAG440.1523387459751411No Hit
ATGGGACGGTGAATGCATGAATGATTGAACGACAGAAAAGATTTGCATTGAC430.14887650174843334No Hit
TTCAATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA430.14887650174843334No Hit
ATCTGAGGGATCTGGCTAAGGGTGCCAAGGCTGGCAAATCAGTGTAATTTCA430.14887650174843334No Hit
CGCAACGGGCATCTTGCTTCCGTTTCTCCAGGTTCATGGATCAACATCCGGA430.14887650174843334No Hit
CGTCTGGGGTTAAGTATTTGAGGTGATGGATATGTTAATTGGCTTGATTTAA420.14541425752172557No Hit
CACGCAGGGCTAATGTAGATGATGGGTTGATGAGTGCAGCAAATCACAATGG420.14541425752172557No Hit
CGAATTGGGTGCTAATTTGATTTGTGGTTTGGTAGGTAATATCTACACATTT420.14541425752172557No Hit
TTGCTGGGATTACAGGCCTGAGCCACTGTGCCCAGCCAAAATGTGCCTTTGC420.14541425752172557No Hit
TATCATGGGTTAAGTGTACTTCAAGTGCAGTATTTATGTAAAACCACTAAGA410.14195201329501783No Hit
TTATTAGGGGTCCTATCAATGGAAACTTTAACAAAGGAGCTAGATTTTGGTT400.13848976906831006No Hit
ATTATAGGGTAGTCACCATGGTTTGCTCTCAGAAAACACATGTGACATGGCC400.13848976906831006No Hit
TCGTTAGGATTTCTAAGCTCAAGGTTAAAAATCATGTCACCTTTTTTTTTTT400.13848976906831006No Hit
TAGACTGGGGTAGATCATGCAGCCACCATTGCTGTACACTAGAGTGGACTTT400.13848976906831006No Hit
CTTGGCGGGTGTTTCTACAAATTGTGGAATTGTTCTTATCTATAATGCTTTT400.13848976906831006No Hit
TTCCCTGGGTTATTTCACTGGGCCACAATTCAAACTTTACTACCTTCATTCT400.13848976906831006No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACTTCCGAT400.13848976906831006No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCACTTCCGATCTCGTATG400.13848976906831006No Hit
TTTTTGGGGTGTGTTTGGGATGCCTTTTCCTCAGATAGCCTCCTGGCTTTCT400.13848976906831006No Hit
GTAGACGGGTAGAAATGAGATTTGGTGGAGTCTGGATTGCCTGTTTTGTATA390.13502752484160233No Hit
TTAAACGGGCCGCTTCTCTCAGCTGGATATAAATACTATTTCTAAAAAATTA390.13502752484160233No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACTTCCGATCCTGTCTCT390.13502752484160233No Hit
AACAACGGGTGATCATGCCACTGCACTCTAGCCTGGGTGACAGAGCAAGTCC390.13502752484160233No Hit
TTACAGCGGGGGGTGAGAGAAACCAAACCCTTCCCCCCATCACTTATGGTTC390.13502752484160233No Hit
AGGCAGGAGGAGAGAGACAGAGGGGGAAGGAGAGAGACAAGTGCTGCTGTGG390.13502752484160233No Hit
GCACACGGGATTAAAATACTAAAAGCAAAGATGTTGTGTGTGTACTGACCCA380.1315652806148946No Hit
ACCGCAGGGTGGGGATGAAAAGATCTTCAGTTAATGGAACAAGAACTAGAGG370.12810303638818682No Hit
GTTAATGGGTGGCTAACCATTTTTGTGAGATAGTAAGTGTTAATGTTAATGA370.12810303638818682No Hit
TGACGCGGGTGTGACTTAGAATAAAGGGATACAATTAGTTAATTTCAGGGCA370.12810303638818682No Hit
CTTAACGGGTAAAGAGGTATATTGGGACAATTAGTCATCTTTCCTACATTTT370.12810303638818682No Hit
TCATAAGGGTGCAAGGACAATTGCAAGGTCCCATCTCCAAGGTGCGTCATAT370.12810303638818682No Hit
TTGCTTGGGTGTTTGCTGAAGTTTTGTCTCTTGGTCCACAGTATTTGGAAAG360.12464079216147907No Hit
GATAACGGGTAGCCTCTTACCCAACAGTTTGCCTTTATATTCAAACTATTTG360.12464079216147907No Hit
TGTATTGGGTGACTACCTGGGGGAGCTGCTCTTAGAAGAAAAGAGATAAAGT360.12464079216147907No Hit
AGCTGTGGGTGACTTGGGAATGGGGTAACTTCTTTATGCATAGTATTAAGGG360.12464079216147907No Hit
ATGTATGGGTAGCTCAACCAACTCCTTTAGCATGTAAACTCACAGAAGGCAA360.12464079216147907No Hit
CTTGCTGGGTGGATATGTTAATTAGCTTGGTAGTAGTAATCATTTCATGATA350.12117854793477131No Hit
TGTGCCTGGGAAGCTCACACCAAATTTTTCTATCAAATAAAACTAGTGACAG350.12117854793477131No Hit
CATTATGGGTATGCCAAAACAAGTTTCTATAACACCCCAGAAAGACCACAGC350.12117854793477131No Hit
ATTTAGGGGAAGAACCCACAGCTAACATCATTTTTAATAATAAAACCTAGAA350.12117854793477131No Hit
CATCGGGGGTGGTTTGAATGCCTTTCTCCCCTGACTATCGAACAACCTGCTA340.11771630370806356No Hit
CAGAGCACTCTGGAGCCTGAGTTTTCTTACGTACTCTGCTTACTGCACTGAC340.11771630370806356No Hit
CATCTGGGTAGTGATGATCTCTGGTTTGTAGGAATGTACCATTTTATTTCCT340.11771630370806356No Hit
CTTCTTGGGTGTCCAAGACAGCCTGGCTGGGAAAGGGGGTGTGGAAAGAGCA340.11771630370806356No Hit
TTGTTAGGGTAAAACAGAGAGGGTCTTTGCTTTACTGTTGTTTATAGTCTTG330.11425405948135581No Hit
TATCCAGGGTGGTTTCATCATGTTGGTCAGGCTGGTCTCGGACTCCTGACCT330.11425405948135581No Hit
GCTCAAGGGGTATTTCTCTGTTTCTATAGTGAATATTCTTATTTATCAAAAT330.11425405948135581No Hit
ACAGTGGGAACAATCCAATGGTTGAAGACACCAAACAGCAGGAGAGTGGTTC330.11425405948135581No Hit
GGACACGGGATTTGTTATTTCAGATCTTCCCAAAAAGAAAACTCTGAGCAAA330.11425405948135581No Hit
TTGTGTCGGGGTTATATGTTTCATAGAATGATACTGCTTTTCACTCTTGATG330.11425405948135581No Hit
TAATAGGGGGGACAAGAGCATCAACCCTCTGGGTGGCTTTGTCCACTATGGT320.11079181525464807No Hit
GCGTATGGGAATGTCTGGTGTTTGTCATGGTGAAATTATAACTATTACAGTG320.11079181525464807No Hit
CGCTCCGGGGTGTCATCACATGAATTCTTTTGATTTACTGTTTTTTTCTAAA320.11079181525464807No Hit
AACAACGGGGTCATGCACACCCCTGAACCACGAGGAAACAGTACAGTCGCTA320.11079181525464807No Hit
ATAGTGGGGGAATTGAGAAGTGCTCATGAAAATGCATAGGTAAATTATAAAA320.11079181525464807No Hit
TCCGTGGGGAAAATGGACTTAACATTCAGATGTTGACAATATAAGCTGCCTT310.10732957102794032No Hit
TATTGGGGGATCTTGCCATGGGCGATCTTTTGCTGGGCGGCTCACTGAAGCC310.10732957102794032No Hit
GATCAAGGGCCTCTCTTTTTCATGTCTCTTTTTTCTACAAATATTAAATCTT310.10732957102794032No Hit
TTTATGGGTACTCTTAAAATATTTAAGCAAGAAGTAATATAGGAAGGACTTA310.10732957102794032No Hit
AAACTGGGCCGTGCTAGCCATTTGGGGGAAATATAGGTGAAGAGTGGCTGGG310.10732957102794032No Hit
CAACACGGGCTTATTGTTGAAACTTATTTTCAACAAAACTGTGCAGTTAAAT310.10732957102794032No Hit
AAGTAGGGGTGTGACTCAGCAATCCTGTATCTACAATCCTTATTTTCATTAT310.10732957102794032No Hit
TTCGAAGGGGTCATCTGGAGCAGGGCAGGTAACCACAGGAGGAGGCATGCCC310.10732957102794032No Hit
GTGTGCGGGTAGCGGGTGCCTGTAATCCCAGCTACTTGGGAGGCTGAGAGAG300.10386732680123256No Hit
TCGCTCGGGATACTCAAGTTGTTTCACAAGATTTATAATATAGATGGTTTTG300.10386732680123256No Hit
GACACAGGGGCCTTTGAGTTTAATGTTAATTACCTTACTATGGTTCCTTGCA300.10386732680123256No Hit
ATTTTAGGGTAAAAAAGAAAGGTTTGGCCAGGCACGGTGGCTCATGTCTGTA300.10386732680123256No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACTTCCGATCTCGT300.10386732680123256No Hit
CTGTCCGGGTAATTCCTGGAGTTGTTTCTGATAAAAAACAATTACACTAAAC300.10386732680123256No Hit
TAGCAGGGGGTTTAAAAAGATGTTTATTCTGTTTGTCAGGTACAAAGATATT300.10386732680123256No Hit
ATATACGGGTAGGGAAGTCCAAAGTCAAGAGCAGAAACAAAAGACACTAGAG300.10386732680123256No Hit
TCGTAGGGTAGTATTTTTATATGCCAACAGTGAATAATCTGTAAAAGAAATA290.10040508257452481No Hit
GAAACAGGGTATTGGTTTATGTTTTATTGTGTCAAAAATCACTTTTAGTTTA290.10040508257452481No Hit
GCTCTAGGGATGAAGAGTTTATAATCTAAGTATAATCCTATGAAGGGCATTA290.10040508257452481No Hit
TTCAAGGGGGAACCTGCAGTATTTCAGACAGATACTAGAGCAGCAAGATACT290.10040508257452481No Hit
TTTTGAGGGGGTTTAACTCTTAAATGGAATGAAACTTTTAAAAAATCTGATT290.10040508257452481No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)