FastQCFastQC Report
Sat 31 Dec 2022
SRR5846545.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846545.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences327392
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACTTCCGATCTCGTATGC17320.5290294203890138No Hit
CTTATACACATCTCCGAGCCCACGAGACCACTTCCGATCTCGTATGCCGTCT15360.46916234972143483No Hit
CTTATACACATCTCCGAGCCCACGAGACCACTTCCGACTGTCTCTTATACAC4370.13347913204965303No Hit
GCGGCCGGGCAACTGAGATTGAAATCCAAGTGCTGGTTTCTAGTTCTGAACA4280.13073013390675398No Hit
CCTAGTGGGCGATCTCTTAGCGGGGGTTCCTGCTCAGTAATGATATTGTGAG4060.12401036066855635No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACTTCCGACTGTCTCTTA3990.1218722510018571No Hit
TTTTATGGGCACTGGCTCTCCCTTCTCTAGGCTCACAATGGACTTTTCCTGC3850.11759603166845861No Hit
GATCCTGGGCGTGAATACAGGTATTTAAAGTCACTAGCCTAGATGAGATCAC3750.11454158928745968No Hit
AATTAAGGGAATCCCTGTCAGAATCTCTGCTGACATCTTTGCAGAAATTGAC3650.11148714690646076No Hit
TTAAAAGGGGTGGCCAATAAGCATATGAAGAAAAGCTCAACATCACTGATCA3570.10904359300166161No Hit
TCAGCGGGATCTTGAATGTTGTGTATATATTTTAAAAAGCACTTTGCAAAAT3560.10873814876356173No Hit
GTACACGGGATGCCAGAAAATGCACATAGGCACAACACTTTTACATACACGT3530.10782181604926204No Hit
TTGACCGGGCAAGAGACTTGTCAAAGGTCTAACAGAGGATTCTTTCATTTAC3520.10751637181116215No Hit
TGTGAGGGCAAAGTGACAGCTGCATGTGTGTTATTACCCCCGAAGACCTTCC3460.1056837063825628No Hit
GCGACAGGGCTAGTCAGGACTCACACCATAGAGAATAAAATAAAATCAAACT3450.10537826214446291No Hit
CTTGTAGGGGATAACACCAATGGAAAAGTGAAAAATGTAGATGTAGACAGAC3360.10262926400156389No Hit
AACACAGGGGATATACAAGATCTCTATGCTGAAAACTGCAAAAACGTAACTG3360.10262926400156389No Hit
ATACACGGGGCACTCTTCAGATTCAGGAAAAAATATGACCACAGATCCAAGG3350.10232381976346398No Hit
TAAGTGGGGGGCACACGCCGCCATATCTGACACATTTTTTTATTTTTAAGAG3350.10232381976346398No Hit
CAAAGCGGGAAAGTAAGAAGTAATTCAAATATCTGCTTGTGGGTCAATAAAA3340.1020183755253641No Hit
GTTAACGGGACTTGCAGACAGAGAAACTGAGGTCTTCCATGACTTGCCTAGT3330.10171293128726419No Hit
TTGGCCGGGAAAGATATACCAGGCAAATACATGTAGAAAATAAAATTTGAAC3310.10110204281106443No Hit
GAGGCAGGAGAATCGCTTGAACCCAGGAGATGGAGGTTGTCGTGAACCGAGA3310.10110204281106443No Hit
GCCATAGGGACATAGCAAAGGATTTGTATATGTTATTTTATTTGTATTGAAA3310.10110204281106443No Hit
TATGAGGGGGGCGCCGGGCTCCCATGAAAAGGTCAACAGCAGGTAATTAGTT3300.10079659857296452No Hit
CTTCGCGGGATCTTATGTGAGAATAAAATATTTAAGGGTTGAACCTTATTTT3300.10079659857296452No Hit
GAGTTTGGGATCCCTAACCCACCAAGAAAACAGAATAACTTATGTTTTTTAA3290.10049115433486464No Hit
ATGAGCAGGGAAAGAGTCAGAAGCACAGATATCCTGGTTTGCTCCTGAGGAT3290.10049115433486464No Hit
GACAAAGGGCAAGGCGCGGACGCTATCTACGACCACATCAACGAGGGGAAGC3290.10049115433486464No Hit
TTACAAGGGGGGCCTGAACCCAGCGGGTGCCGCTTCTCCACCCGAGGCTTCC3280.10018571009676473No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)