FastQCFastQC Report
Sat 31 Dec 2022
SRR5846544.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846544.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences328259
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACTTCCGATCTCGTATGC15770.48041333215540166No Hit
CTTATACACATCTCCGAGCCCACGAGACCACTTCCGATCTCGTATGCCGTCT14500.44172437008581633No Hit
TCAGCGGGATCTTGAATGTTGTGTATATATTTTAAAAAGCACTTTGCAAAAT4400.13404049850879945No Hit
CTTATACACATCTCCGAGCCCACGAGACCACTTCCGACTGTCTCTTATACAC4270.13008021105285766No Hit
GATCCTGGGCGTGAATACAGGTATTTAAAGTCACTAGCCTAGATGAGATCAC4200.1279477485765813No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACTTCCGACTGTCTCTTA4140.12611992359691584No Hit
CCTAGTGGGCGATCTCTTAGCGGGGGTTCCTGCTCAGTAATGATATTGTGAG4080.12429209861725041No Hit
GTTAACGGGACTTGCAGACAGAGAAACTGAGGTCTTCCATGACTTGCCTAGT3900.11880862367825407No Hit
GCGGCCGGGCAACTGAGATTGAAATCCAAGTGCTGGTTTCTAGTTCTGAACA3840.11698079869858861No Hit
TTTTATGGGCACTGGCTCTCCCTTCTCTAGGCTCACAATGGACTTTTCCTGC3770.11484833622231226No Hit
CATAAGGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA3670.11180196125620318No Hit
CTTAGTGGGATGCCTGTGTTGATAAAACTTTGCTAAATATTTTATATGTATT3620.11027877377314864No Hit
TCTGCAGGGAGAGCAACCTCCACCCAACCTCTCCTTTCACCCCAGTCATCAC3610.10997413627653774No Hit
ATACACGGGGCACTCTTCAGATTCAGGAAAAAATATGACCACAGATCCAAGG3570.1087555862900941No Hit
ATTCGCTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCC3560.10845094879348319No Hit
TTGACCGGGCAAGAGACTTGTCAAAGGTCTAACAGAGGATTCTTTCATTTAC3550.10814631129687229No Hit
CAAAGCGGGAAAGTAAGAAGTAATTCAAATATCTGCTTGTGGGTCAATAAAA3530.10753703630365047No Hit
GAGTTTGGGATCCCTAACCCACCAAGAAAACAGAATAACTTATGTTTTTTAA3520.10723239880703957No Hit
TACTTAGGGACAGACTCTCATGGACTGTGGCTTTCTTGGCTAGAACATAGGA3510.10692776131042865No Hit
GCCATAGGGACATAGCAAAGGATTTGTATATGTTATTTTATTTGTATTGAAA3490.10631848631720683No Hit
TTCCTTGGGGAACTTACTTTGCCCTTCAAATTTCCGAATGATGGTCCCCAGA3450.10509993633076321No Hit
AGCTAAGGGTAGGGCCAGAATCTGTATATTTCAAAATTTCCTTCAGCGTTTC3420.10418602384093048No Hit
TGAGCCCGGGGGATGATGGCAGAAAGATTGTATTTGTCCCAGGGTGTTCCAT3400.10357674884770866No Hit
CGCTAAGGGCAGGTTCTTTGAGAGAGAAGTGAAGATGGGCGCAAAGCTGCTT3400.10357674884770866No Hit
GCTGAGGGGAAGCACTCTGAGCTGCCTCAAAGGCTAGTGAGTTCTCTCTCAC3370.10266283635787594No Hit
GCTGGGAGTACAGGGATGAGCCACTGAGCCGAGCCAATTTTGCTTGTTTTAA3350.10205356136465413No Hit
ATGAGCAGGGAAAGAGTCAGAAGCACAGATATCCTGGTTTGCTCCTGAGGAT3350.10205356136465413No Hit
AATAATGGGATGAAAGGAAATGTTAGTTATGCGAAAGGTTCTAGGTAGTCAA3340.10174892386804323No Hit
GGTGCTGGGATGTGGATAATTTTAGTGCATTGCTCACCCGGTATGTTTTTTT3340.10174892386804323No Hit
AATTAAGGGAATCCCTGTCAGAATCTCTGCTGACATCTTTGCAGAAATTGAC3330.10144428637143231No Hit
TGGTGCGGGATTATTTAGCCACCTTTTGTAAATATTTCATGTAGATATTGGC3330.10144428637143231No Hit
GCGACAGGGCTAGTCAGGACTCACACCATAGAGAATAAAATAAAATCAAACT3330.10144428637143231No Hit
CACTCCGGGTGATGCGGCTGATGGAAACACTAGCATAGGACAAAAATACAAA3320.10113964887482141No Hit
GAAACGGGGTGGTGGAATCAGATTGGTAGGGCAATTTTTCTTGCTCCTGAGA3300.10053037388159959No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)