FastQCFastQC Report
Sat 31 Dec 2022
SRR5846543.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846543.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences322479
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACTTCCGATCTCGTATGC16370.5076299541985679No Hit
CTTATACACATCTCCGAGCCCACGAGACCACTTCCGATCTCGTATGCCGTCT14360.4453003141289821No Hit
CTTATACACATCTCCGAGCCCACGAGACCACTTCCGACTGTCTCTTATACAC4070.12620976869811676No Hit
GATCCTGGGCGTGAATACAGGTATTTAAAGTCACTAGCCTAGATGAGATCAC3690.11442605565013535No Hit
GCGGCCGGGCAACTGAGATTGAAATCCAAGTGCTGGTTTCTAGTTCTGAACA3650.11318566480297942No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACTTCCGACTGTCTCTTA3520.10915439454972263No Hit
CTTGTAGGGGATAACACCAATGGAAAAGTGAAAAATGTAGATGTAGACAGAC3510.10884429683793363No Hit
TTGACCGGGCAAGAGACTTGTCAAAGGTCTAACAGAGGATTCTTTCATTTAC3510.10884429683793363No Hit
GCGGCTGGGAATTTCTGAACGAATGAGCACACTTTAACCAGTTATTTACAGT3510.10884429683793363No Hit
GCCATAGGGACATAGCAAAGGATTTGTATATGTTATTTTATTTGTATTGAAA3510.10884429683793363No Hit
CCTAGTGGGCGATCTCTTAGCGGGGGTTCCTGCTCAGTAATGATATTGTGAG3470.10760390599077768No Hit
TCAGCGGGATCTTGAATGTTGTGTATATATTTTAAAAAGCACTTTGCAAAAT3370.10450292887288785No Hit
GTACACGGGATGCCAGAAAATGCACATAGGCACAACACTTTTACATACACGT3370.10450292887288785No Hit
TATGAGGGGGGCGCCGGGCTCCCATGAAAAGGTCAACAGCAGGTAATTAGTT3350.10388273344930987No Hit
TTGGCCGGGAAAGATATACCAGGCAAATACATGTAGAAAATAAAATTTGAAC3350.10388273344930987No Hit
AATTAAGGGAATCCCTGTCAGAATCTCTGCTGACATCTTTGCAGAAATTGAC3300.10233224489036497No Hit
CATAAGGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA3270.101401951754998No Hit
TCTAGCGGGCAAGCCCAGAGCTCAAGGAAACCTGCTTGGTGACTTCCCCAGC3230.10016156090784208No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)