FastQCFastQC Report
Sat 31 Dec 2022
SRR5846542.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846542.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164468
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACTTCCGATCTCGTATGC10130.6159252863778972No Hit
CTTATACACATCTCCGAGCCCACGAGACCACTTCCGATCTCGTATGCCGTCT10130.6159252863778972No Hit
ACCTTTGGGCGATCTGTAAGAGGGAAGGGAGTCGGATTACTCGCTAGTTTTT2550.15504535836758518No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACTTCCGACTGTCTCTTA2460.14957316924872924No Hit
CTTATACACATCTCCGAGCCCACGAGACCACTTCCGACTGTCTCTTATACAC2440.1483571272223168No Hit
ATCTTGGGGGACACACATAGGCTCAAAGCGATGGAGGAAAATTTACCAAGCA2410.14653306418269815No Hit
TTCCCTGGGCTCCTTCTGCTTGAGGAAAGGAGAGGGAAGAGTACAGATGACT2310.14045285405063598No Hit
GACGGGGATCTCTTGGCGGGTTACATAGTAAGATGTAAACAGAGTAGATACT2290.13923681202422356No Hit
TTTAGCGGGAGAGAGCGAGTGAGCAAGCGAGCAGAAAAGAGGTGGAGAGGGG2280.13862879101101733No Hit
ATAGCAGGGAAAGATAATCAGAAAGAATCTGCTTTATTTAGAACTTGACTAG2250.13680472797139867No Hit
TTTCATGGGAGATGAAGTTGGATCACCATCCTACAACAATAAAGTCCAGGCT2220.13498066493178004No Hit
TGAATAGGCACATACATGACTGTTAACATCAGTAATATTTTATGATAGTTTA2160.13133253885254276No Hit
TATAATGGGAACAAAAATGGAAAACAAATTCAGAAATAAGAATATCTGCCTA2090.12707639176009922No Hit
GATTCAGGGACTCTAGTCTTCATAAAAGACAGACAGGGGGGTTGTGTATCCA2060.1252523287204806No Hit
TCCACTGGGGGCCGCGCGGCCGGCCAGTTCCAGCCCGCACCCCGCGTCGGTG2040.12403628669406815No Hit
GCACAATGGGGCAGTTATTTTGGTAAGTTAAAGGACTTCATTTTTTGAAAAC2030.12342826568086193No Hit
TCGACAGGGGCTTGTTGGAAAGTCCGTGTGTAACTTATCTCTTATTATTGCT2000.12160420264124329No Hit
GTTTGAGGGAGTGATCTAAAAGTTTTCTATTGTCACTGGGTTACCTTCAGAA2000.12160420264124329No Hit
GTGCATGGGATGATGTTCATAGAGTTAACTTAAAAGGAGAACTCTGAGTCAA1990.12099618162803706No Hit
TGCGGTCGGGGTCTATTTTCACTAACAATATTAACCTATAATTTTCTTTTTT1990.12099618162803706No Hit
ACAAAAGGGGGGGGAAGGTATAGGGACATGAAGGGAGATAAGTTTACTTCAC1980.12038816061483086No Hit
AAATGAGGGAAGGGATTAATAAACATAATATATAAGGAGCTCAAGCAACTGT1970.11978013960162463No Hit
TGCACCTGGGGTACTTCTTAAAAAACATAGGTAATAGAAAATATACACAAGT1960.1191721185884184No Hit
ATTGAAGGGTAGAGGTTGGCCCTTTGTAAAGTCTAAGAAAATGGACACATCT1960.1191721185884184No Hit
GCTGCGGGGATAGTACCCATGAGCATTTCATGTTAGAAACCCCATTTAAAGC1940.11795607656200598No Hit
CAGTGGGAAGAGGTATCCGATGAATATAATGTAGCAGGATAAACAAAATGGC1940.11795607656200598No Hit
GATCACGGGACAGATAGGAGGAGACAAGTCATGTTTCGAGATAACTAAGAAA1940.11795607656200598No Hit
CTTATAGGGATAGCAACAGAAAACAGCATGTGACAATGAGTTTTGCCAATCT1930.11734805554879976No Hit
AATCTGGGGAATAATGAAGGTTCTTTTACTGAAGGATGCGAAGGAAGATGAC1930.11734805554879976No Hit
AGCTTGGGGACAGTAACAGCTTCTTGACGATATACTTACAATTTTAAAGTGT1920.11674003453559356No Hit
GTAAAAGGGGCCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAG1910.11613201352238733No Hit
GATAATGGGGCGTTCCCTGTGCGCCGGAAGTGATCCCCTGCGTGGCTGGGCT1910.11613201352238733No Hit
GCTACAGGGGGATGTGTCCTGCTCCATTGAGATGGATGGCAAACCCCATTTT1910.11613201352238733No Hit
CAAGCAGGGGCCAAAATAAGCAATACACACCTACCTGGAAAATCGTGTTGAG1890.1149159714959749No Hit
TTAGAAGGGCGCTATAATTTGCAAGAAAGAGAAAATGGTGGAATTTTTATTG1860.11309190845635625No Hit
TTCACTGGGCTGGATTAAGGGACACACACAGCTGGTCAAACAATTTCAGCCA1850.11248388744315002No Hit
AAGCCTGTGTAAAATGCTACCAAATGGCAAAAAGCAACAATAAACAGTTTGA1840.11187586642994382No Hit
CACAAAGGGGAATCGTATCCTCCTGTGTTTTTTCAGACTCCTTGGAAATTAA1830.11126784541673759No Hit
GTATATGGGATGACAGCTTAGACCAGAGATATTGAGAAGTGGCTGGATTCTG1830.11126784541673759No Hit
GGCTTGGGGAGAGGAGACTGCAGACTTCGGTTGAGGAAACGGGTATTTCATG1830.11126784541673759No Hit
AGTGAGGGGCACATTGAATTTTGATCAAATAGTAGATAAAAATATCATTTAT1820.11065982440353139No Hit
GTTTTCGGGGGATACATTCTGAGAAATGCATCATTGGGCGATTTCATGATCG1810.11005180339032516No Hit
TGTGCAGGGCAACTGCGCTTTATAGAGAATGATGTTATATATTTAATTACTT1800.10944378237711895No Hit
CTCATCCGGGATATTAAAGCTTATTACCTAACTGAGAATGACATGGAACGGT1800.10944378237711895No Hit
AGACCTGGGATCTAAGATTCAACATTCATGAAGGAGCAGAAATACTTGGATT1800.10944378237711895No Hit
AGTATAGGGCAGGTTCTTACAAAGTAAATATTGAAAAAGGCAAATACTGGGC1800.10944378237711895No Hit
GTCATAGGGCAATGTGGTACCTGCACAAGTCTAGAAGGACCTATTGCCTATG1800.10944378237711895No Hit
AACAATGGGGCTTTAGCTGCCATCTTGCGTCCCCGCGTGTGTGCGCCTAATC1800.10944378237711895No Hit
GTCAAAGGGGCGTCGTAGTCTCCTGCAGCGTCTGGGGTTTCCGTTGCAGTCC1790.10883576136391275No Hit
GCAGCTTGGGACATTTGTGTATGTTCACATATTTACAGTTTTGTTAAAAATG1780.10822774035070652No Hit
ATCTAAGGGGTAAAATGGCACAAGAATATATGAAAGTTCAAACAGAAATTGC1770.1076197193375003No Hit
TTCGCGCGGGAAGGTTCACGTGTCTGGGAGCAGATGTCGGTTTAATGGCATC1760.1070116983242941No Hit
GAGTGAGGGCAAGGATTGTTTTCTCAATGCACCTGAGTCAGCCATCTGTATT1760.1070116983242941No Hit
CTACGGGCGGATCGCTTGAGCTCAGGAGTTCGAGACCGGCCTGGGCAACATG1750.10640367731108787No Hit
GAATTTGGGATAACATTTTCTTTCTTGAATTCACATATTAGTTTGTGTAGAG1750.10640367731108787No Hit
TGTCATGGGATCCAATATTTTTAAAGGTTGTAAGACTATAAGGAAATAGCCA1750.10640367731108787No Hit
TCAGCAGGGGCCTGAATTTGTATACATCTTGAATATGATGTTCTGATGCTTC1740.10579565629788167No Hit
AGCATGGGCTTGGAACTGTGACTCACTTTTGGAATTTTAAGGACGTAATTAC1730.10518763528467544No Hit
CATGCAGGGTGGAAATAGAATAGCATAAAAGGTCCAGAAATAAATCCAAGCA1730.10518763528467544No Hit
TCGCTGGGGGTACTTTTGTCTTTTAACATTTATATTAGGGTTAAAAGTGACT1730.10518763528467544No Hit
AATCCAGGGGAGAAAAGAAGAGATGAGGCAGCCAAGCTGAGCCTCACTTGGA1730.10518763528467544No Hit
GCCATAAGGTGAGCTGGAATACACCTTCATAATCTGTTAAAGGCCAAAATTA1720.10457961427146921No Hit
AGTTATGGGAATCCTACTGTGAAGCTGTCGTTGAGAACTTAGGTTGGCACGT1720.10457961427146921No Hit
AAGTAGGGACTAATAAAAAATATTTCCTGCAAAAGAAAGACATATTTTCCAT1710.10397159325826301No Hit
GCTGCTGACCTGGGAGAGTGGGCAGGTCCTGGGGGACCAGACGGTCTCAGAC1700.1033635722450568No Hit
TCTCATGGGCTCATGTCTTAGCATCTGATTAATAATAAAGTCATTGACTTCA1700.1033635722450568No Hit
CATAAAGGGAATGAGGCTTTCCTGTGATTCCTCATATTATCAAGTCTTTGGT1700.1033635722450568No Hit
ATGTCAGGGGCAATATGGTCTAGCCTTACACCTCATTGAAGCTGTGGAAGCC1700.1033635722450568No Hit
TTCGTAGGGAACTTGCCAAATTCCTAAGTTGTTTTGGTGAGGCTGAAATGAG1690.10275555123185057No Hit
TCTGTGAGGGGGTGGGGAGAGTTTTTATTTTAGTAGTTCATGGACTCCCTTT1690.10275555123185057No Hit
GGCGCAGGGGAACAGAGAATGTAGAGTGTTATAAGAATCCATTGAGTAAAAT1690.10275555123185057No Hit
TACATAGGGATGACTTACAAGTTTATCTGAGTCATCTCCATGCACACCACTC1690.10275555123185057No Hit
CTTCGCGGGACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCT1670.10153950920543814No Hit
TTATATGGAGTTGAGAGTTGTACTTCATCCTGTTTGTTCTGGGCGAGGGAAG1670.10153950920543814No Hit
TTCTGCGGGAGAGACAGCTTGAAGACCTTGTTGAAGAATTGAGCTTTTCAAG1660.10093148819223194No Hit
GGCCTAGGGGGGGTTAAGGCAAAATTTGGAAAGGGACAATTTTTGGGGATAT1660.10093148819223194No Hit
AATTAAGGGCAGATGTTGCTTCATCAAGACTGCAGAGGTTAGACAACTCCAC1660.10093148819223194No Hit
TCTGTCGGGGAGATGCGCCACGGTTTCGGTAGCGACGGTAGCTCTAGCCGGG1660.10093148819223194No Hit
GCGTACGGGAAATGGAAACCAGAAAATCCACTTTATAGTTTTTCACCAATAT1650.10032346717902571No Hit
ACTTGCGGGCATGGAGGGGATGTGTGCTCCCTGTTTCTCTGGGACAAATGAG1650.10032346717902571No Hit
CTGTGAGGGATCGCATGATGAGCTATGAACCACTGGAGCAGCCCACACTGGC1650.10032346717902571No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)