FastQCFastQC Report
Sat 31 Dec 2022
SRR5846541.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846541.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164639
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACTTCCGATCTCGTATGC10020.6086042796664217No Hit
CTTATACACATCTCCGAGCCCACGAGACCACTTCCGATCTCGTATGCCGTCT9660.5867382576424783No Hit
CTTATACACATCTCCGAGCCCACGAGACCACTTCCGACTGTCTCTTATACAC2490.15123998566560778No Hit
GACGGGGATCTCTTGGCGGGTTACATAGTAAGATGTAAACAGAGTAGATACT2480.1506325961649427No Hit
ATAGCAGGGAAAGATAATCAGAAAGAATCTGCTTTATTTAGAACTTGACTAG2460.14941781716361252No Hit
TTTAGCGGGAGAGAGCGAGTGAGCAAGCGAGCAGAAAAGAGGTGGAGAGGGG2390.14516609065895686No Hit
ACCTTTGGGCGATCTGTAAGAGGGAAGGGAGTCGGATTACTCGCTAGTTTTT2330.14152175365496633No Hit
TTCCCTGGGCTCCTTCTGCTTGAGGAAAGGAGAGGGAAGAGTACAGATGACT2270.13787741665097578No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACTTCCGACTGTCTCTTA2260.13727002715031067No Hit
TATAATGGGAACAAAAATGGAAAACAAATTCAGAAATAAGAATATCTGCCTA2240.13605524814898048No Hit
CAGTGGGAAGAGGTATCCGATGAATATAATGTAGCAGGATAAACAAAATGGC2240.13605524814898048No Hit
TCCACTGGGGGCCGCGCGGCCGGCCAGTTCCAGCCCGCACCCCGCGTCGGTG2240.13605524814898048No Hit
GCCATAAGGTGAGCTGGAATACACCTTCATAATCTGTTAAAGGCCAAAATTA2130.1293739636416645No Hit
CCTTGGGCATGGTAAAGAACATCTGATTTAGGTGGCATAACTAATTCTAAAA2090.12694440563900414No Hit
TGCGGTCGGGGTCTATTTTCACTAACAATATTAACCTATAATTTTCTTTTTT2020.12269267913434848No Hit
GCTGCGGGGATAGTACCCATGAGCATTTCATGTTAGAAACCCCATTTAAAGC2010.12208528963368338No Hit
AAGTAGGGACTAATAAAAAATATTTCCTGCAAAAGAAAGACATATTTTCCAT2010.12208528963368338No Hit
GTGCATGGGATGATGTTCATAGAGTTAACTTAAAAGGAGAACTCTGAGTCAA2000.12147790013301829No Hit
GATTCAGGGACTCTAGTCTTCATAAAAGACAGACAGGGGGGTTGTGTATCCA1990.1208705106323532No Hit
AGCTTGGGGACAGTAACAGCTTCTTGACGATATACTTACAATTTTAAAGTGT1980.1202631211316881No Hit
CCAATAGGGGGGATGGTGGGCGCGCCCCGGGCCTGGGCTGGAGTGACTGATC1980.1202631211316881No Hit
CAAGCAGGGGCCAAAATAAGCAATACACACCTACCTGGAAAATCGTGTTGAG1950.11844095262969283No Hit
TTAGAAGGGCGCTATAATTTGCAAGAAAGAGAAAATGGTGGAATTTTTATTG1950.11844095262969283No Hit
GCACAATGGGGCAGTTATTTTGGTAAGTTAAAGGACTTCATTTTTTGAAAAC1950.11844095262969283No Hit
TTTCATGGGAGATGAAGTTGGATCACCATCCTACAACAATAAAGTCCAGGCT1940.11783356312902776No Hit
GCACAAGGGACTATTCGCACATTTAAGCATCTTTGTTTTACATAAAATTAGA1940.11783356312902776No Hit
ATCTTGGGGGACACACATAGGCTCAAAGCGATGGAGGAAAATTTACCAAGCA1930.11722617362836267No Hit
AACAATGGGGCTTTAGCTGCCATCTTGCGTCCCCGCGTGTGTGCGCCTAATC1930.11722617362836267No Hit
CACAAAGGGGAATCGTATCCTCCTGTGTTTTTTCAGACTCCTTGGAAATTAA1900.11540400512636739No Hit
TCAGCAGGGGCCTGAATTTGTATACATCTTGAATATGATGTTCTGATGCTTC1900.11540400512636739No Hit
TTCACTGGGCTGGATTAAGGGACACACACAGCTGGTCAAACAATTTCAGCCA1890.1147966156257023No Hit
GTATATGGGATGACAGCTTAGACCAGAGATATTGAGAAGTGGCTGGATTCTG1880.11418922612503721No Hit
ATCATGGGGGGATAGAACAATGGTTGTTAAAGTGCAGTTTTATTTAATACAA1880.11418922612503721No Hit
GTTTGAGGGAGTGATCTAAAAGTTTTCTATTGTCACTGGGTTACCTTCAGAA1880.11418922612503721No Hit
GAGCATGGGCCTGAAGTGATTCTGTCCTGTTGCTTTCTCCTGCAGGGTGTGG1880.11418922612503721No Hit
TCGACAGGGGCTTGTTGGAAAGTCCGTGTGTAACTTATCTCTTATTATTGCT1870.11358183662437212No Hit
TTCGCGCGGGAAGGTTCACGTGTCTGGGAGCAGATGTCGGTTTAATGGCATC1860.11297444712370702No Hit
GTTTTCGGGGGATACATTCTGAGAAATGCATCATTGGGCGATTTCATGATCG1850.11236705762304193No Hit
GGCTTGGGGAGAGGAGACTGCAGACTTCGGTTGAGGAAACGGGTATTTCATG1850.11236705762304193No Hit
CATAGTGGGAAGGTACACTTTTCTCAAGATGGACAGAGCTCAAAGCCAGGGC1840.11175966812237684No Hit
CATGCAGGGTGGAAATAGAATAGCATAAAAGGTCCAGAAATAAATCCAAGCA1840.11175966812237684No Hit
CATAAAGGGAATGAGGCTTTCCTGTGATTCCTCATATTATCAAGTCTTTGGT1840.11175966812237684No Hit
AAGCCTGTGTAAAATGCTACCAAATGGCAAAAAGCAACAATAAACAGTTTGA1830.11115227862171174No Hit
CTACGGGCGGATCGCTTGAGCTCAGGAGTTCGAGACCGGCCTGGGCAACATG1820.11054488912104665No Hit
TAACCAGGGATTCACAAAACGCCGCATGTTTAAAAAGTCATAGATGTCATCT1820.11054488912104665No Hit
AATCTGGGGAATAATGAAGGTTCTTTTACTGAAGGATGCGAAGGAAGATGAC1820.11054488912104665No Hit
CTTATAGGGATAGCAACAGAAAACAGCATGTGACAATGAGTTTTGCCAATCT1810.10993749962038156No Hit
TGAATAGGCACATACATGACTGTTAACATCAGTAATATTTTATGATAGTTTA1800.10933011011971647No Hit
GATAATGGGGCGTTCCCTGTGCGCCGGAAGTGATCCCCTGCGTGGCTGGGCT1790.10872272061905137No Hit
ATGTTTGGGGGGCTTCACTAACCTTCCCTAGGCATTGAAACTTCCCCCAAAT1790.10872272061905137No Hit
TGCACCTGGGGTACTTCTTAAAAAACATAGGTAATAGAAAATATACACAAGT1790.10872272061905137No Hit
AGTGAGGGGCACATTGAATTTTGATCAAATAGTAGATAAAAATATCATTTAT1790.10872272061905137No Hit
TAGTTAGGGATCTTGGTTTTGACTTAAAATAAGAATGCTTCTAAATAATTAT1790.10872272061905137No Hit
AAATGAGGGAAGGGATTAATAAACATAATATATAAGGAGCTCAAGCAACTGT1780.10811533111838628No Hit
TGTCATGGGATCCAATATTTTTAAAGGTTGTAAGACTATAAGGAAATAGCCA1780.10811533111838628No Hit
GCCACTGGCTCGCGGGGGCGTCGTGAGGCAGTACTGGAGCAGCAGCTTCCTG1780.10811533111838628No Hit
GCGTACGGGAAATGGAAACCAGAAAATCCACTTTATAGTTTTTCACCAATAT1760.1069005521170561No Hit
TAGAACGGGGTTCACTCTGATGGTAGTTTCTTTTGCTGTGCAGAAGCTCTTT1760.1069005521170561No Hit
GTGTATGGGACACCAAAGTATTAATGATGTGTATCTCAAAGTAATGGAAATA1760.1069005521170561No Hit
TGTTACGGGAGGATGACTGTATGAGTTGTCTGTAAAATGGTTTGGTTGAAAT1760.1069005521170561No Hit
GCAGCTTGGGACATTTGTGTATGTTCACATATTTACAGTTTTGTTAAAAATG1760.1069005521170561No Hit
GAGTGAGGGCAAGGATTGTTTTCTCAATGCACCTGAGTCAGCCATCTGTATT1760.1069005521170561No Hit
TCGCTGGGGGTACTTTTGTCTTTTAACATTTATATTAGGGTTAAAAGTGACT1750.106293162616391No Hit
ATGTCAGGGGCAATATGGTCTAGCCTTACACCTCATTGAAGCTGTGGAAGCC1750.106293162616391No Hit
GTCATAGGGCAATGTGGTACCTGCACAAGTCTAGAAGGACCTATTGCCTATG1750.106293162616391No Hit
ACTTAGGGAATCTAAAAACTAAAACTATAGAACTTCTGGAAGAAAATGTAGG1740.10568577311572591No Hit
GATCACGGGACAGATAGGAGGAGACAAGTCATGTTTCGAGATAACTAAGAAA1730.10507838361506083No Hit
AAAGTAGGGGGGCGGCCAAGCAGACCCCATCCCAGTACCTGGCTCAGCACGA1730.10507838361506083No Hit
AACTTGGGGAAGAATGGCCAGATGGGATTAACAAAGGGAGGTTTTCCCTGAG1730.10507838361506083No Hit
AGCTGAGGGTATTCAAATGTGTGCATATTTTGAATTAATATTTAAATAATCA1720.10447099411439575No Hit
AGCCGTGGGAATATTTGCGTCATCATAGTATGAGATACTTAATCAAGTTTGA1720.10447099411439575No Hit
TTATATGGAGTTGAGAGTTGTACTTCATCCTGTTTGTTCTGGGCGAGGGAAG1710.10386360461373065No Hit
TCTAATGGGTATGAGAATTGACACATAGTATGTATGCATGCATAATCATGAT1710.10386360461373065No Hit
TCTCATGGGCTCATGTCTTAGCATCTGATTAATAATAAAGTCATTGACTTCA1710.10386360461373065No Hit
CCATATGGGTAACCAGGAGATATTTGGGGAGCAAAATTTTAAGGAATTTCAA1710.10386360461373065No Hit
CTTGCTGGGTCGGGAAATGAATTGTTTTTATTTGTTTGTGTGTCTGTTTGCA1710.10386360461373065No Hit
AGTTATGGGGGTCTGGGAGTCAGCAGGCATGGAATTTAGTTCCTTTGTATTA1700.10325621511306557No Hit
GAATTTGGGATAACATTTTCTTTCTTGAATTCACATATTAGTTTGTGTAGAG1700.10325621511306557No Hit
ACAAAAGGGGGGGGAAGGTATAGGGACATGAAGGGAGATAAGTTTACTTCAC1700.10325621511306557No Hit
TTCTGCGGGAGAGACAGCTTGAAGACCTTGTTGAAGAATTGAGCTTTTCAAG1690.10264882561240046No Hit
AAAGAAGGGGGGGTGAGGATGGCTAAGACCACTGATACTTATGTCATAGCAT1690.10264882561240046No Hit
ATGTAAGGGGAGCTGACAACAGTTACTGGAAAGACCATTTGTAGAAGAATTT1690.10264882561240046No Hit
CGGCTGGGAAGATGGTCAGCCTGTCCTTTGAAACTTTGAAGCCAAGCATTGA1690.10264882561240046No Hit
ATTGAAGGGTAGAGGTTGGCCCTTTGTAAAGTCTAAGAAAATGGACACATCT1690.10264882561240046No Hit
TTCGTAGGGAACTTGCCAAATTCCTAAGTTGTTTTGGTGAGGCTGAAATGAG1680.10204143611173538No Hit
GTACTAGGGTAAACTTATATTCTTTATTATTATTTAGTACAAAAATAGATTC1680.10204143611173538No Hit
AATCCAGGGGAGAAAAGAAGAGATGAGGCAGCCAAGCTGAGCCTCACTTGGA1680.10204143611173538No Hit
GCCGCAGGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAAC1680.10204143611173538No Hit
CTGTGAGGGATCGCATGATGAGCTATGAACCACTGGAGCAGCCCACACTGGC1680.10204143611173538No Hit
AGACCTGGGATCTAAGATTCAACATTCATGAAGGAGCAGAAATACTTGGATT1670.10143404661107028No Hit
AAGCATGGGATGCCAGCAACCTTGTAAGCTATCTGTGCAGGATATTTGCACT1670.10143404661107028No Hit
TGCAAAGGGGAATGAATGAATAATTAAGTTAAATTCCAGAATGAAGGTTACT1660.1008266571104052No Hit
TTTCAGGGGTGAACATACCAAAGTTTATTTATAAGGTATCTCTGACTGTATA1660.1008266571104052No Hit
CACTAAGGGCGATCTTTCTTCGGGATCAGTAGAAGGAGAAATCAGTAATTGG1660.1008266571104052No Hit
ATCTAAGGGGTAAAATGGCACAAGAATATATGAAAGTTCAAACAGAAATTGC1660.1008266571104052No Hit
CATTATGGGCTTGAAGGAAATGTTTCACCTTATTTTTGGTCTTTGTTTATTC1650.10021926760974009No Hit
TAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAAGATAGAGATTA1650.10021926760974009No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)