FastQCFastQC Report
Sat 31 Dec 2022
SRR5846540.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846540.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159634
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCACTTCCGATCTCGTATGCCGTCT10310.6458523873360312No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACTTCCGATCTCGTATGC9640.6038813786536703No Hit
CTTATACACATCTCCGAGCCCACGAGACCACTTCCGACTGTCTCTTATACAC2610.163499003971585No Hit
GACGGGGATCTCTTGGCGGGTTACATAGTAAGATGTAAACAGAGTAGATACT2560.16036683914454314No Hit
TTTAGCGGGAGAGAGCGAGTGAGCAAGCGAGCAGAAAAGAGGTGGAGAGGGG2460.15410250949045942No Hit
ACCTTTGGGCGATCTGTAAGAGGGAAGGGAGTCGGATTACTCGCTAGTTTTT2420.15159677762882595No Hit
TTCCCTGGGCTCCTTCTGCTTGAGGAAAGGAGAGGGAAGAGTACAGATGACT2280.14282671611310874No Hit
ATAGCAGGGAAAGATAATCAGAAAGAATCTGCTTTATTTAGAACTTGACTAG2250.1409474172168836No Hit
GTTTGAGGGAGTGATCTAAAAGTTTTCTATTGTCACTGGGTTACCTTCAGAA2150.1346830875627999No Hit
TTTCATGGGAGATGAAGTTGGATCACCATCCTACAACAATAAAGTCCAGGCT2140.13405665459739152No Hit
GATTCAGGGACTCTAGTCTTCATAAAAGACAGACAGGGGGGTTGTGTATCCA2130.13343022163198318No Hit
TGCGGTCGGGGTCTATTTTCACTAACAATATTAACCTATAATTTTCTTTTTT2120.1328037886665748No Hit
GTTTTCGGGGGATACATTCTGAGAAATGCATCATTGGGCGATTTCATGATCG1990.12466016011626596No Hit
TGAATAGGCACATACATGACTGTTAACATCAGTAATATTTTATGATAGTTTA1990.12466016011626596No Hit
TTAGAAGGGCGCTATAATTTGCAAGAAAGAGAAAATGGTGGAATTTTTATTG1980.12403372715085759No Hit
GCTGCGGGGATAGTACCCATGAGCATTTCATGTTAGAAACCCCATTTAAAGC1980.12403372715085759No Hit
GAGTGAGGGCAAGGATTGTTTTCTCAATGCACCTGAGTCAGCCATCTGTATT1980.12403372715085759No Hit
TCGCTGGGGGTACTTTTGTCTTTTAACATTTATATTAGGGTTAAAAGTGACT1960.12278086122004084No Hit
GATCACGGGACAGATAGGAGGAGACAAGTCATGTTTCGAGATAACTAAGAAA1950.12215442825463246No Hit
ATCTTGGGGGACACACATAGGCTCAAAGCGATGGAGGAAAATTTACCAAGCA1950.12215442825463246No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACTTCCGACTGTCTCTTA1930.12090156232381571No Hit
TATAATGGGAACAAAAATGGAAAACAAATTCAGAAATAAGAATATCTGCCTA1890.11839583046218224No Hit
CAGTGGGAAGAGGTATCCGATGAATATAATGTAGCAGGATAAACAAAATGGC1890.11839583046218224No Hit
AAATGAGGGAAGGGATTAATAAACATAATATATAAGGAGCTCAAGCAACTGT1880.11776939749677387No Hit
GTCAAAGGGGCGTCGTAGTCTCCTGCAGCGTCTGGGGTTTCCGTTGCAGTCC1860.11651653156595713No Hit
CAACTTGGGAGTTCTGCTTTCAAAAATTAAAATTCAAACTTGAAAAAGCTGT1840.11526366563514039No Hit
CCTTGGGCATGGTAAAGAACATCTGATTTAGGTGGCATAACTAATTCTAAAA1830.11463723266973201No Hit
ATGTTTGGGGGGCTTCACTAACCTTCCCTAGGCATTGAAACTTCCCCCAAAT1830.11463723266973201No Hit
GGCTTGGGGAGAGGAGACTGCAGACTTCGGTTGAGGAAACGGGTATTTCATG1830.11463723266973201No Hit
TACAATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1820.11401079970432364No Hit
TCAGCAGGGGCCTGAATTTGTATACATCTTGAATATGATGTTCTGATGCTTC1810.11338436673891526No Hit
AGCTTGGGGACAGTAACAGCTTCTTGACGATATACTTACAATTTTAAAGTGT1810.11338436673891526No Hit
AACAATGGGGCTTTAGCTGCCATCTTGCGTCCCCGCGTGTGTGCGCCTAATC1810.11338436673891526No Hit
GCCATAAGGTGAGCTGGAATACACCTTCATAATCTGTTAAAGGCCAAAATTA1800.11275793377350689No Hit
GATGTGGGGCTGTAGAACGTGCTGAGTTTTCCTTTTGCTGTTTCCAGGCTGC1800.11275793377350689No Hit
TCTCATGGGCTCATGTCTTAGCATCTGATTAATAATAAAGTCATTGACTTCA1800.11275793377350689No Hit
GGCGCAGGGGAACAGAGAATGTAGAGTGTTATAAGAATCCATTGAGTAAAAT1790.11213150080809853No Hit
AATCTGGGGAATAATGAAGGTTCTTTTACTGAAGGATGCGAAGGAAGATGAC1790.11213150080809853No Hit
GTGCATGGGATGATGTTCATAGAGTTAACTTAAAAGGAGAACTCTGAGTCAA1770.11087863487728178No Hit
ATCATGGGGGGATAGAACAATGGTTGTTAAAGTGCAGTTTTATTTAATACAA1760.1102522019118734No Hit
CTTGCTGGGTCGGGAAATGAATTGTTTTTATTTGTTTGTGTGTCTGTTTGCA1760.1102522019118734No Hit
CTCATCCGGGATATTAAAGCTTATTACCTAACTGAGAATGACATGGAACGGT1750.10962576894646503No Hit
AGTGAGGGGCACATTGAATTTTGATCAAATAGTAGATAAAAATATCATTTAT1750.10962576894646503No Hit
CTTATAGGGATAGCAACAGAAAACAGCATGTGACAATGAGTTTTGCCAATCT1740.10899933598105666No Hit
TGACATGGGAATATAGTATCGTCTGCATTCCAGCAAAAATATCAACTCGTAA1740.10899933598105666No Hit
TGTTACGGGAGGATGACTGTATGAGTTGTCTGTAAAATGGTTTGGTTGAAAT1730.10837290301564828No Hit
TGCTCTGGGCTGACTTCTGGAGCTGGGAGCTAATAAATGAGTGTTGCTTTAA1730.10837290301564828No Hit
CTTTACGGGGTTAGAAGAATTCCTATTGGAGGTGTTAAATCTACAAGCAAGA1730.10837290301564828No Hit
AGCTGAGGGTATTCAAATGTGTGCATATTTTGAATTAATATTTAAATAATCA1720.10774647005023993No Hit
TAAGGTGGGAGGTTGTAAGTAAAAGAATAGAAAAAAGATGTGTTGTGCAAAA1720.10774647005023993No Hit
TAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAAGATAGAGATTA1710.10712003708483156No Hit
TACATAGGGATGACTTACAAGTTTATCTGAGTCATCTCCATGCACACCACTC1710.10712003708483156No Hit
TATCCAGGGTAGTCTCGAACTCCTGACCTCAGGTGATCCACCCGCCTCCGCC1700.10649360411942319No Hit
CAAGCAGGGGCCAAAATAAGCAATACACACCTACCTGGAAAATCGTGTTGAG1690.10586717115401481No Hit
GATATAGGGTACTACCTACCTACCTATGAAATAATTTCAAAAGTATCAATAT1690.10586717115401481No Hit
TTTGTAGGGTCTGATATGAGTAATTAGTATTGTGGGATTTTTAAATGTTTTG1690.10586717115401481No Hit
TTTCAGGGGTGAACATACCAAAGTTTATTTATAAGGTATCTCTGACTGTATA1690.10586717115401481No Hit
TCCACTGGGGGCCGCGCGGCCGGCCAGTTCCAGCCCGCACCCCGCGTCGGTG1690.10586717115401481No Hit
TTCTGCGGGATCTTACAAAATACACATTCTTGCTTGTGTGTCTGTGTGTGTG1680.10524073818860644No Hit
TGTTCCGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG1670.10461430522319808No Hit
AATTAAGGGCAGATGTTGCTTCATCAAGACTGCAGAGGTTAGACAACTCCAC1670.10461430522319808No Hit
CCAATAGGGGGGATGGTGGGCGCGCCCCGGGCCTGGGCTGGAGTGACTGATC1670.10461430522319808No Hit
GCGTACGGGAAATGGAAACCAGAAAATCCACTTTATAGTTTTTCACCAATAT1660.1039878722577897No Hit
GAATAGGGGATCATTCTGGAGCAAGAGATGAGCTGTGTTTTACTGGCCACTT1660.1039878722577897No Hit
ACTTGCGGGCATGGAGGGGATGTGTGCTCCCTGTTTCTCTGGGACAAATGAG1660.1039878722577897No Hit
GCACAATGGGGCAGTTATTTTGGTAAGTTAAAGGACTTCATTTTTTGAAAAC1660.1039878722577897No Hit
CACAAAGGGGAATCGTATCCTCCTGTGTTTTTTCAGACTCCTTGGAAATTAA1650.10336143929238133No Hit
AAGTAGGGACTAATAAAAAATATTTCCTGCAAAAGAAAGACATATTTTCCAT1650.10336143929238133No Hit
AGCATGGGCTTGGAACTGTGACTCACTTTTGGAATTTTAAGGACGTAATTAC1640.10273500632697295No Hit
TTATATGGAGTTGAGAGTTGTACTTCATCCTGTTTGTTCTGGGCGAGGGAAG1640.10273500632697295No Hit
ACAAAAGGGGGGGGAAGGTATAGGGACATGAAGGGAGATAAGTTTACTTCAC1640.10273500632697295No Hit
GAGCATGGGCCTGAAGTGATTCTGTCCTGTTGCTTTCTCCTGCAGGGTGTGG1640.10273500632697295No Hit
TCGACAGGGGCTTGTTGGAAAGTCCGTGTGTAACTTATCTCTTATTATTGCT1630.10210857336156458No Hit
TAATGGGGATTCTGACTTTGCTTTACAGTCTAGCACTTAGTTTCTTAGCTTG1620.1014821403961562No Hit
GCTACAGGGGGATGTGTCCTGCTCCATTGAGATGGATGGCAAACCCCATTTT1610.10085570743074783No Hit
ATTTGAGGGTAATCCAAATATCTTTGACCCTGGCCACTTTCTAGATAAGAAT1610.10085570743074783No Hit
AATCCAGGGGAGAAAAGAAGAGATGAGGCAGCCAAGCTGAGCCTCACTTGGA1610.10085570743074783No Hit
GCAGCTTGGGACATTTGTGTATGTTCACATATTTACAGTTTTGTTAAAAATG1600.10022927446533947No Hit
TGTGCAGGGCAACTGCGCTTTATAGAGAATGATGTTATATATTTAATTACTT1600.10022927446533947No Hit
AAGTACGGGCACTAAGTTTTACTAGAGTCTTTTGAATTAAAAATATGCCATT1600.10022927446533947No Hit
AAGCCTGTGTAAAATGCTACCAAATGGCAAAAAGCAACAATAAACAGTTTGA1600.10022927446533947No Hit
GTATATGGGATGACAGCTTAGACCAGAGATATTGAGAAGTGGCTGGATTCTG1600.10022927446533947No Hit
AGCCGTGGGAATATTTGCGTCATCATAGTATGAGATACTTAATCAAGTTTGA1600.10022927446533947No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)