FastQCFastQC Report
Sat 31 Dec 2022
SRR5846535.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846535.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69633
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCACTTCCGATCTCGTATGCCGTCT7601.0914365315295909No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACTTCCGATCTCGTATGC6380.9162322462051039No Hit
CTTATACACATCTCCGAGCCCACGAGACCACTTCCGACTGTCTCTTATACAC1870.26855083078425457No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCACTTCCGATCTCGTATGCC1730.24844542099292005No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACTTCCG1340.19243749371705943No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACTTCCGACTGTCTCTTA1220.17520428532448695No Hit
ATGTAAGGGCACTGCAAATGACAATAACATCTTATGTAATGAAATAGTTAAA1070.15366277483377133No Hit
CTCTGCAGGGGGTTTATTGCTCCTTAATGAGGTACAGACTGTGCTGCATTCA930.1335573650424368No Hit
ATCTAAGGGTACACCAGAGGTGGCCGAATCATCTCCACTTCTTCGCGTTCGG890.12781296224491262No Hit
TGATATGGGTATGTACAGTTGACCTGTTTAGAAGTTGTATTGCCATTTAGTT890.12781296224491262No Hit
GACACTGGGGAAGTCACATCAGAAGGTTTGCAATCCCACAGAGAAACCCAAA890.12781296224491262No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCACTTCCGA880.12637686154553157No Hit
AGGACTGGGACAACAAACAAACAAAACCCCCAAAAAAACAAAACTTAGTTGA870.12494076084615052No Hit
TTTGTTGGGACTTTTCCTTTGCTGTTGATGTTTATCCATGCATCGTTGAGAC860.12350466014676949No Hit
GCTTATGGGCATCAAGAAATAGGTTATAGGTAAAATCTCTGAAGGATCATCT840.1206324587480074No Hit
AGTGATGGGTCTGAATTCTTCATAGTTCCTAATTTCTAACTATCTTCCAAGC810.11632415664986429No Hit
GGGCTGGGGGTAACTATGTGAGATGATAGCTACAGTAATCTATTTCACTATG800.11488805595048325No Hit
CAATGGGGTGTGAATTAATTTCCTCAAATGCTCAGTTGCCTGAAGATGAATT790.1134519552511022No Hit
TTGCGAGGGGTCACCTCAGGACCACTACGATAATTGTGTTAACTGTACAAAT790.1134519552511022No Hit
AGAGCTGGGGAGACTGCGGGACGGACGGTGGACGCTGGGACGCGTTTGTAGC770.11057975385234012No Hit
TACATAGGGGCTGTAGTGGCTTCGTCTTCGGTTTTTCTCTTCCTTCGCTAAC770.11057975385234012No Hit
AGTCTAGGGATATCAAACACTAGAAGTTCTTACCACAGTAGAGATTATCTTA760.10914365315295908No Hit
CCCGTGGGGAAGAGGTTTGTAGTCTCAAATGAGTAGGTTTTTCCTCTAGAGA760.10914365315295908No Hit
TAGCATGGGGACAGCCCTGTCAAGGATGAGGTGGTAGTAAATGACCGATGGG750.10770755245357805No Hit
TAAATCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA740.106271451754197No Hit
TTTAAAAAGAGATTAAACCGAAGGTGATTAAAAGACCTTGAAATCCATGACG740.106271451754197No Hit
AAAGCCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC730.10483535105481596No Hit
ACACACGGGTGATGCCCCGGAAAAAGTGGGGCCCAAACAAGCTGAAGATGCT720.10339925035543493No Hit
AACTGTGGGTGAAACAGATGTTGCTGGTAACACAACAGAGGACAAAAACATG720.10339925035543493No Hit
CCCTCAGGGGTGACGGCATTGATTGAACATTTTTTACTAGTAGTCTTATGAC720.10339925035543493No Hit
TTGTCTGGGAATACACACGAGGAAAAGCAGAGGTTTATTTTATCTGCCTTTT710.10196314965605388No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)