FastQCFastQC Report
Sat 31 Dec 2022
SRR5846534.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846534.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68458
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCACTTCCGATCTCGTATGCCGTCT7051.0298285079903007No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACTTCCGATCTCGTATGC6310.9217330333927372No Hit
CTTATACACATCTCCGAGCCCACGAGACCACTTCCGACTGTCTCTTATACAC1930.2819246837476993No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCACTTCCGATCTCGTATGCC1550.22641619679219377No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACTTCCG1530.22349469747874612No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACTTCCGACTGTCTCTTA1170.17090770983668818No Hit
AAATTTAGGGAGTGGGGGATACATATTTACATAGTCACATAACTATAGCTAC930.13584971807531626No Hit
CTCTGCAGGGGGTTTATTGCTCCTTAATGAGGTACAGACTGTGCTGCATTCA920.13438896841859244No Hit
CAATGGGGTGTGAATTAATTTCCTCAAATGCTCAGTTGCCTGAAGATGAATT900.13146746910514476No Hit
ATCTAAGGGTACACCAGAGGTGGCCGAATCATCTCCACTTCTTCGCGTTCGG890.13000671944842093No Hit
ACAACGGGACCTACAGACAACCTAAGGCAGTATCCTTTTCCCACCGATCTTT850.1241637208215256No Hit
ATGTAAGGGCACTGCAAATGACAATAACATCTTATGTAATGAAATAGTTAAA840.12270297116480178No Hit
TGATATGGGTATGTACAGTTGACCTGTTTAGAAGTTGTATTGCCATTTAGTT820.11978147185135411No Hit
TTTAAAAAGAGATTAAACCGAAGGTGATTAAAAGACCTTGAAATCCATGACG790.11539922288118262No Hit
GCTTATGGGCATCAAGAAATAGGTTATAGGTAAAATCTCTGAAGGATCATCT780.11393847322445878No Hit
AGAGCTGGGGAGACTGCGGGACGGACGGTGGACGCTGGGACGCGTTTGTAGC770.11247772356773496No Hit
AATCCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA770.11247772356773496No Hit
TAGCATGGGGACAGCCCTGTCAAGGATGAGGTGGTAGTAAATGACCGATGGG770.11247772356773496No Hit
GACACTGGGGAAGTCACATCAGAAGGTTTGCAATCCCACAGAGAAACCCAAA750.1095562242542873No Hit
CCCGTGGGGAAGAGGTTTGTAGTCTCAAATGAGTAGGTTTTTCCTCTAGAGA740.10809547459756347No Hit
AACTGTGGGTGAAACAGATGTTGCTGGTAACACAACAGAGGACAAAAACATG730.10663472494083963No Hit
TTGAATGGGTGGTGACATTGATGGCAAATGATGTTATCAGTTTGTTTATATT720.1051739752841158No Hit
TACAGTGGGTCTGTCAAAGGGACTTGTGAATAATAGGAATATATTTTGCAGA720.1051739752841158No Hit
AACTCCGGGCTATGTGGGGCAATTAGTCAATCAATTTCCATCAAAATTATAC720.1051739752841158No Hit
TCCACTGGGACCTCATTAAACATCTGTTGTTTGTTAAAGATGTTCCACTGAA710.10371322562739196No Hit
CCCTCAGGGGTGACGGCATTGATTGAACATTTTTTACTAGTAGTCTTATGAC710.10371322562739196No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCACTTCCGA700.10225247597066814No Hit
GGGCTGGGGGTAACTATGTGAGATGATAGCTACAGTAATCTATTTCACTATG700.10225247597066814No Hit
CACAGTGGGATCACAGATTTGTGTGTTTTGAATCATAAGATTTTAGATCTGG690.10079172631394431No Hit
ACAATGGGGCGTCTGGTAAGACAACCAGCAAGTTAACAATGGTCACCTCCAG690.10079172631394431No Hit
CTTATACACATCTCCGAGCCCACGAGACCACTTCCGATCCTGTCTCTTATAC690.10079172631394431No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)