FastQCFastQC Report
Sat 31 Dec 2022
SRR5846518.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846518.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83972
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGC48085.72571809650836No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGCCGTCT39084.6539322631353315RNA PCR Primer, Index 30 (95% over 22bp)
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCG10591.2611346639355976No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGA9551.1372838565236032No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATG5330.6347353879864717No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTCTTATAC5310.6323536416900871No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTCT4920.5859095889105892No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGAT3760.4477683037202877No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGAACTCGTATGC3650.4346686990901729No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGCC3380.40251512408898205No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGT2600.3096270185299862No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGACCTCGTATGC2260.2691373314914495No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTT1710.2036393083408755No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGACCTCGTATGCCGTCT1490.17744009908064592No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTC1420.16910398704330015No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGT1400.16672224074691563No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCTGTCTC1360.16195874815414663No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTC1300.1548135092649931No Hit
GTAGCTGGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGA1210.1440956509312628No Hit
ATCTGAAGACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCAC1200.14290477778307056No Hit
CTACATGGGATTGCTGGTTATGATGATATGGGCCCTCACATTTTCCAAACCT1180.14052303148668605No Hit
TGATAGGGGATGTTACCACATGAGGACCTAAGAAAAGGTGCATTGCTGATTT1170.1393321583384938No Hit
CACAGAGGGGAGAAAAAGCAGGGTAAAAAGATTTTATTTAATTAATTGAAAA1150.13695041204210928No Hit
CGCTCGGGCGCCTCACTGTTGAGCCCTACCCCTTCCAGTATATTGTATTTCT1140.135759538893917No Hit
CCTACTTGCAGATTTAACAAAATTTTAGGGAAATTGAAAAAGACATGTAGAA1110.13218691944934025No Hit
TCGACTGGGGCTGATATATCATACAATTGGCATATAGAGCTTCAGTTCATTA1100.130996046301148No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTC1090.12980517315295576No Hit
GCTCAAGGTTATCTTTTTATAATTAAGTCAGTTCAGTTTATTTAATAGATAT1080.12861430000476348No Hit
ACGACAGGGGTTAGAAATTTAGGCTATTTCCAGATTTTCAGTATTGTAAATA1070.12742342685657124No Hit
TCTACTGGCAAGCAATCTGTTTATTTTTAGTCCCTTTCCCTACCACTTCCAG1070.12742342685657124No Hit
TTATTTGGGCGCTCTTCCGATCTTCTAGAGGGATCTCCCTGGATCTCACCTT1070.12742342685657124No Hit
ATGCATGGGATTTCCACTGGGTGAATGGGGACCGCTCCTCTGCCAACCTGCT1070.12742342685657124No Hit
GCCGCTGGGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAAT1060.126232553708379No Hit
TCGGTCGGGGAATATTCTTATGGATGACACTAAATAATTCCATTTAACAGTT1060.126232553708379No Hit
AGCGAAGGGATCTGAATCATGTTTTGAGCTTGTTTGCAGTACTTTTAAACAT1060.126232553708379No Hit
ACATTGGGGCACCCACTTTTAAAAATCCACTCATGTCCATGGTATTCAAAAT1060.126232553708379No Hit
GCGTGAGGGATACAAGTTTATGTTTAATAATTTTTTGGAAATTACAGATTTT1060.126232553708379No Hit
TCCAGAGGGGTTGCTCAGTAATCAACAGCTACTACAACAATCTTAACAGTAT1050.12504168056018672No Hit
TGCACTGGGGTTGGAGATGTAGTAGTGAATAAATCAAAGTTCTTGCCCTCAT1030.12265993426380223No Hit
CGCCATTCCAATGGGATAAAAATAAAATAAAATAAAAGCATATATGTTGTTA990.1178964416710332No Hit
ACCCATAAAATTATTTGAAAACTTTCACCAGAAAGGGATGTAAGTCTCGCTT980.11670556852284096No Hit
TTTACTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC980.11670556852284096No Hit
AGTAATGGGTGTGTATTACATGCTAATTCATATAAGCTGTATCTGTCAGCTA980.11670556852284096No Hit
ATAACTGGGATAGCATCTTCGTATAATGTTATCTCATAATGTTGTTGCCAAG970.11551469537464869No Hit
CGATGGGATTCAGGTAACTGAAGATTTTGTGTCTCCAGAACATCTTGTAGAG970.11551469537464869No Hit
GACGTCGGGACATCATACTTGGACATATCAAGCATTGGTGCACGATGTACTG970.11551469537464869No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGAACTCGTATGCCGTCT950.11313294907826418No Hit
ACGTAAGGGGACCTATGAAAGGAGAAGTGGTACCCGTTCCCGGGGCATCCCA950.11313294907826418No Hit
GCGACGGGGAGCCAGAGCCTCCTCCAAAGGAAAATTGCAACAGTATATACTA940.11194207593007192No Hit
GCTATGGGGTCCTGAATGGTATTGCCTAGATTTTCTTCTGGGTTTTTATAGT930.11075120278187967No Hit
TTCTGCGGGATGAGTGGGCTGTAGTTGGGCCTAAATAAATGAGCTGTTGGAA930.11075120278187967No Hit
GCTTAGGGATGGAGATGAATGGGAAGCTGTTTGATGAGCTCACAGCCTCCTA920.10956032963368742No Hit
GTGATGGGGGACAATTTCAAGTGAGACAGGTAAACATTTTTAGTTATGTGAT920.10956032963368742No Hit
GCAACAGGGAGGAAAAAAGAGGGATCGCAATCCCAGTTTCTGATAAAACAAA920.10956032963368742No Hit
GAAAAGGGGGCCTTTCCGTCTGGCGGCAGCCATCAGGTAAGCCAAGATGGGT920.10956032963368742No Hit
GTCTAAGGGAATTGTAATTAAGTATGTTGCAGTTGTAAATATTAGAGTTTAA910.10836945648549517No Hit
GGGTTTGGGAAATGTGGAAACAAATGGAAAAGTGGGCTTACCACTAATACAA900.1071785833373029No Hit
AATATGGGCAGGTCCACAGATGCATTACGAAAAATACAGGAAAGCTATGGAG890.10598771018911066No Hit
AGCTAGGGTATATTACTTTGCTTTCTGGGCTGAGCTCAGGACAGATGCCAAA890.10598771018911066No Hit
AGGGCGGGGGATCAGGTGCCTATAAATCCCTCTCTTTATCTGGTGAAATATG890.10598771018911066No Hit
CTTGACGGGTAGATAGACCTGAGTTTCAACCATAGCTGCACTATCGTTTAAC880.10479683704091841No Hit
GTGCCGGGGGCCATGTGTAATATAGCAAACTTCTATGATTAACAATACGTTC880.10479683704091841No Hit
ATAAGGGGGAAGAAGACTTATTTTTGCATACTGGCACTTTAAGAAATTACAT870.10360596389272615No Hit
ACGCCGGGATAACAGATATAATAATGAAAAAGATTGAAATGTGAGAATTACC870.10360596389272615No Hit
ATCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCG860.10241509074453388No Hit
ATGGCTGGGGTTTTGATAATATCACATCCTGGAAAGTGAAACAGAAAATTAT860.10241509074453388No Hit
TTTGAAGGGGTGCAGGGATTTGAAGAAACAAATAAATCCGAGAGAACTGAGA860.10241509074453388No Hit
TTTGTAGGGTGGTTATGGTGGTCAGAGCAGTATGAGTGGATATGACCAAGTT860.10241509074453388No Hit
GTCGATGGGGTTTTTGTTCTCCCTTTCCCAGTGTTAATGGGGGACTTTGAAA860.10241509074453388No Hit
TCCAAAGGGACCGTCAGGCACTGGTGTTGATTCCTCAGAACGCATCCACCTA860.10241509074453388No Hit
TTCGGGGGGATGGCCACTGAGAGAATTCAGAATAAATTGAAAGATGGAGTCT860.10241509074453388No Hit
GGTTGGGGTTTTGGGTTTTTTGTTGTTGTTGTTTATTTGTTATTTTAAAGGT850.10122421759634163No Hit
AAGATGGGCAAGTAAGCGGCAACAAGGGTCCGTGGACCAGGAGGATTGCCTT840.10003334444814939No Hit
GATCATGGGGCGTTATATTTTAATTTTGGCTTTCACGTGTTCATTGCTATTA840.10003334444814939No Hit
ATACTGGGTGTAACAGGAGATGAAACATGGCTTTACCAGCGCAATCCTGAAG840.10003334444814939No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)