FastQCFastQC Report
Sat 31 Dec 2022
SRR5846516.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846516.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81487
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGC46335.685569477339944No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGCCGTCT37074.549191895639796RNA PCR Primer, Index 30 (95% over 22bp)
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCG10781.32291040288635No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGA9301.141286340152417No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATG6080.746131284744806No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTCTTATAC4980.6111404273074232No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTCT4850.5951869623375508No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGAACTCGTATGC3850.47246800103083925No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGAT3840.47124081141777213No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGCC3520.4319707437996245No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGT2640.32397805784971834No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTT1720.21107661344754378No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGT1490.18285125234700014No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGACCTCGTATGC1460.17916968350779877No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTC1450.17794249389473168No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCTGTCTC1360.16689778737712763No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTC1330.16321621853792628No Hit
ACATTGGGGCACCCACTTTTAAAAATCCACTCATGTCCATGGTATTCAAAAT1150.14112680550271822No Hit
ATCTGAAGACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCAC1150.14112680550271822No Hit
GCTATGGGGTCCTGAATGGTATTGCCTAGATTTTCTTCTGGGTTTTTATAGT1140.1398996158896511No Hit
TGATAGGGGATGTTACCACATGAGGACCTAAGAAAAGGTGCATTGCTGATTT1140.1398996158896511No Hit
CGCTCGGGCGCCTCACTGTTGAGCCCTACCCCTTCCAGTATATTGTATTTCT1120.13744523666351688No Hit
AGGGCGGGGGATCAGGTGCCTATAAATCCCTCTCTTTATCTGGTGAAATATG1120.13744523666351688No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGAACTCGTATGCCGTCT1080.13253647821124842No Hit
GCCGCTGGGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAAT1070.1313092885981813No Hit
TTATTTGGGCGCTCTTCCGATCTTCTAGAGGGATCTCCCTGGATCTCACCTT1060.1300820989851142No Hit
ACGCCGGGATAACAGATATAATAATGAAAAAGATTGAAATGTGAGAATTACC1040.12762771975897996No Hit
ATGTTGGGGCTGGGAAGTCCAAGTTTGAGGGGTGAGCATCTGGTCAGGGCCT1040.12762771975897996No Hit
CAGTTTGGGGGATTTTCAGATCTTTTCATGAGAACTGGAAATGTAGCTGGGT1030.12640053014591285No Hit
AATATCGGGGACGAATTATGCTTTTAAATCATCCTGACAAAGGAGGATCTCC1030.12640053014591285No Hit
TTTGTAGGGTGGTTATGGTGGTCAGAGCAGTATGAGTGGATATGACCAAGTT1030.12640053014591285No Hit
CGCTCTGGGGATTATAAGACAAGGTTTCAAAAAAGTTGAATAGAAGATCCTT1030.12640053014591285No Hit
TCGGTCGGGGAATATTCTTATGGATGACACTAAATAATTCCATTTAACAGTT1030.12640053014591285No Hit
AAGATGGGCAAGTAAGCGGCAACAAGGGTCCGTGGACCAGGAGGATTGCCTT1020.12517334053284573No Hit
TCGACTGGGGCTGATATATCATACAATTGGCATATAGAGCTTCAGTTCATTA1010.12394615091977862No Hit
CACAGAGGGGAGAAAAAGCAGGGTAAAAAGATTTTATTTAATTAATTGAAAA1000.12271896130671149No Hit
GTCGATGGGGTTTTTGTTCTCCCTTTCCCAGTGTTAATGGGGGACTTTGAAA1000.12271896130671149No Hit
GCAACAGGGAGGAAAAAAGAGGGATCGCAATCCCAGTTTCTGATAAAACAAA1000.12271896130671149No Hit
GCTAAGGGAATCTGATGTGCAAATTATAATTTAAATATGTTAATTTTTTTGC1000.12271896130671149No Hit
ATGCATGGGATTTCCACTGGGTGAATGGGGACCGCTCCTCTGCCAACCTGCT1000.12271896130671149No Hit
GACGTCGGGACATCATACTTGGACATATCAAGCATTGGTGCACGATGTACTG990.12149177169364439No Hit
GAAAAGGGGGCCTTTCCGTCTGGCGGCAGCCATCAGGTAAGCCAAGATGGGT980.12026458208057728No Hit
GGCTACGGGAATAAATAAATCAACACTTTCAGTTTTCTGCCAGTGTCACAGA980.12026458208057728No Hit
AAACCAGCATTCTTCCCTGCTATTTTTTTCTAACTTTTGTCACCATCTGACA970.11903739246751015No Hit
GGGTTTGGGAAATGTGGAAACAAATGGAAAAGTGGGCTTACCACTAATACAA960.11781020285444303No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTC960.11781020285444303No Hit
ATCAATGGGATACTTAAAAGATTTTCATCTTGGAAATACATCAAGTGAAAAT950.11658301324137593No Hit
GTAGCTGGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGA930.11412863401524169No Hit
GAAGCGGGACCAATGAATCAATGAACATAGGTGAGTGAGTCAAGGCAAATTT930.11412863401524169No Hit
GTGCAGGGATTCAGAGCAGAACTAAGTGAAATTGAAACAAACAAACAAAAAA930.11412863401524169No Hit
CCTACTTGCAGATTTAACAAAATTTTAGGGAAATTGAAAAAGACATGTAGAA930.11412863401524169No Hit
GTGAGGGGGTATGAGCTTCTACAGGTATTTTTAAATAGAGCAAGCATGTTGA920.11290144440217459No Hit
CTACATGGGATTGCTGGTTATGATGATATGGGCCCTCACATTTTCCAAACCT920.11290144440217459No Hit
ACCCATAAAATTATTTGAAAACTTTCACCAGAAAGGGATGTAAGTCTCGCTT920.11290144440217459No Hit
AGCGAAGGGATCTGAATCATGTTTTGAGCTTGTTTGCAGTACTTTTAAACAT920.11290144440217459No Hit
TCCAGAGGGGTTGCTCAGTAATCAACAGCTACTACAACAATCTTAACAGTAT910.11167425478910747No Hit
TTCTGCGGGATGAGTGGGCTGTAGTTGGGCCTAAATAAATGAGCTGTTGGAA910.11167425478910747No Hit
CTACATGGGCCGATCTCGGTGCGGGAAGGAAATGGCCCAATGAACGTGGTTG900.11044706517604035No Hit
ATCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCG890.10921987556297323No Hit
CGACTGGGGCAGTTAGTCATGCTCCAAGGATTGAGGTCATGACCATGGGAAT890.10921987556297323No Hit
AATATGGGCAGGTCCACAGATGCATTACGAAAAATACAGGAAAGCTATGGAG890.10921987556297323No Hit
GCGACGGGGAGCCAGAGCCTCCTCCAAAGGAAAATTGCAACAGTATATACTA890.10921987556297323No Hit
GATACAGGGTCTGCTGTGAGAGGCCGTTTCCATTCTTTCCTGCTGAATATTT890.10921987556297323No Hit
ATAAGGGGGAAGAAGACTTATTTTTGCATACTGGCACTTTAAGAAATTACAT880.10799268594990613No Hit
ATGGTCAGGGAGTTTCATGCTTTGCAGAATTTCTTAGACTATAAAGTGACAC860.10553830672377189No Hit
TACTGCGGGAGCCAGCTCTTGTCCCCTGATAAGTGAGGACAGCCTGACCCTG860.10553830672377189No Hit
CTTCAGGGTATTTGGATCAGTGGGCCATTAAAATGCTGATTTGAAATTCAAT860.10553830672377189No Hit
TTTGGTGGGATACTTGGTGGGTGAATCCAATTTTGTAGAATTCCTACACAGG850.10431111711070477No Hit
AGCTTTGGGATCTCAAATATGTGATACAGTGCATAAAAATTAAGAATTAATT850.10431111711070477No Hit
CAAACAGGGATTATTTAAGTAGTACATCTATTTAAATAACACACTAATAGTA840.10308392749763765No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGACCTCGTATGCCGTCT840.10308392749763765No Hit
ATTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATC840.10308392749763765No Hit
GTTAGTGGGGATCCCAGGGTAGACAAGTTAGCAAGTTTGCACTATTGTTCTC840.10308392749763765No Hit
GTCTAAGGGAATTGTAATTAAGTATGTTGCAGTTGTAAATATTAGAGTTTAA840.10308392749763765No Hit
GCAGTGGGGGTGGATGTTTTTAAATGATGTCCAGTGATAAGGTTTTATGAGA830.10185673788457054No Hit
GCTAGAGGGGGCTGCGGCGGGGACCTGACAGCAGGCAGGGACCGCTTCAGGT830.10185673788457054No Hit
AGAAGCGGGTAGGGAAGGGTAAATACATGTACAATTGCTTTTAGTTAGCAAT830.10185673788457054No Hit
TGCACTGGGGTTGGAGATGTAGTAGTGAATAAATCAAAGTTCTTGCCCTCAT830.10185673788457054No Hit
ATCGCTGGGATAATTTAAAATTACATGTTTGTAAATTCACCTGCTCTTTTAT830.10185673788457054No Hit
CGCCATTCCAATGGGATAAAAATAAAATAAAATAAAAGCATATATGTTGTTA830.10185673788457054No Hit
CTTGCCGGGATTTAGCAAACATTTATGTTCTTTATGAAACATTTATGTTCTT820.10062954827150343No Hit
GAGGCAGGGCCTTCAGCTATCATTTGGTTAATAAATACATTTTAGTATTTGC820.10062954827150343No Hit
AAATTAGGGGGATTGGTGAAAAGATAGAGAAAATGTTATTAATAAAGGAACA820.10062954827150343No Hit
TCCAAAGGGACCGTCAGGCACTGGTGTTGATTCCTCAGAACGCATCCACCTA820.10062954827150343No Hit
GCAGCTTGGGTACTCACCAGACGCCTCAACCGCCTTTTCATCAATCGCCCAC820.10062954827150343No Hit
ATCGTAGGGAGCTCTGGAAGCTGTCACTCCTTCCCCATCTTTCCAGCAGAGG820.10062954827150343No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)