FastQCFastQC Report
Sat 31 Dec 2022
SRR5846515.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846515.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93016
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGC65096.997720822224133No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGCCGTCT52345.626988905134601RNA PCR Primer, Index 30 (95% over 22bp)
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCG16191.7405607637395717No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGA10831.1643158166336975No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTCTTATAC7100.7633095381439753No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTCT6780.7289068547346693No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATG6490.6977294228949859No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGAT4670.5020641610045584No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGAACTCGTATGC4570.49131332243915027No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGCC4530.487012987012987No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGT3750.4031564462028038No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGT3140.3375763309538144No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGACCTCGTATGC2660.28597230583985556No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGACCTCGTATGCCGTCT2080.2236174421604885No Hit
GCTTATGGGATAGGATGGTCAACGTGTAACAAGACTGTGCTCAGAAAACGAA2010.21609185516470283No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTC1950.209641352025458No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTT1830.19674034574696828No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTC1810.19459017803388665No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCTGTCTC1620.17416358475961125No Hit
ATCTGAAGACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCAC1540.16556291390728478No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTC1470.1580373269114991No Hit
CTCACAGGGCAACAAAAAGTTGTTTAAAAAAAAGATTAAAAATTTGATAACT1390.1494366560591726No Hit
CGCTACGGGGCGTATGACAGTATGCAAAAATGAAGACTATTGTGTAAAATCA1310.14083598520684615No Hit
ATTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATC1300.13976090135030533No Hit
ATAAGCGGGCCTGAATTCAGAGCCCTTCAAGCTCAGGAAACCACGCTTCACT1300.13976090135030533No Hit
GCATCTGGGAGTCGAGGTGAGACTAGCATCGAAAGGCACATCAACAAGAAGG1280.1376107336372237No Hit
TTTGACAGGGCGATCTAGCAAAGGGAGAAGGAGATGATCAAGAAGGTGAAAA1270.13653564978068292No Hit
CCCAAACCTAGAGAAAGATGTCAGCATTGAAGTACAAGAAGGTTATAGAACT1220.13116023049797884No Hit
CTATGCGGGGAAACCCAGTGTGGAGAAGGCACATGTCTGTGGCTCTGAGCTG1150.12363464350219318No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGAACTCGTATGCCGTCT1140.12255955964565236No Hit
ATCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCG1130.12148447578911156No Hit
CGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCG1130.12148447578911156No Hit
CACACTGGGTCAAGTGGGTTTTATCCCAGTGATGCAGTGATGGTTTAACATA1110.11933430807602995No Hit
TGGCTTAGGGGTAGCCTAGGATCCTCCTGGTGTAAACTGTGCATTTCATACA1100.11825922421948912No Hit
CCAATGGGATGTGGTTCTCTAATTCTCAAGTTTTCTCAGATAAGCATATTCT1100.11825922421948912No Hit
GTGAATGGGGGTCATCCTTTACGGCAGGCGTCCGCGTCGCTAGCTAGTCGTT1090.11718414036294832No Hit
TAGCCTGGGCGACAGAACAAGACTCTGTCTCCAAAATAAAAAATAAATTAAA1090.11718414036294832No Hit
CTTAAAGGGATTTTTGATAACTGTGTACTTCTGGTGACTGTACAGTTTGAAA1060.11395888879332589No Hit
GAATTTGGGAGGCAGAAGGATCACTTGAGCCCAGGATTTCGAGGTTACAGTG1060.11395888879332589No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTAT1060.11395888879332589No Hit
CAAAGAGGGGTACCTAATAAGAGATATATGAATGGAAAAACAGTTTTTGCTC1030.11073363722370345No Hit
ATTTTTGGGGAGATATCTTCTCACTCTGATTAAAATGGCTTATATCCAGAAA1020.10965855336716264No Hit
AAGTCAGGGACCGCTTTTAATCTAGACTCCTCCTGGATCAGATTATAGGACT1020.10965855336716264No Hit
TATTCTGGGGGAATAATTGAGAGTATTCACCTAAAAAACTTCATGTGTCATT1000.10750838565408102No Hit
AAGCTAGGGAAGGAGAGGAGGGCGGGGAAGTTTGCGTCTTATTGAACTTATT1000.10750838565408102No Hit
CCAACTTAAAATTTGAGCTACACAGTTTCAGAAAATGAAAACTGATATTCCA1000.10750838565408102No Hit
AAGAGGGGTGCCTCGGCAAGTCCCCATTTGCTCAGGCAGCCCTGAATGGGCC990.10643330179754022No Hit
GATAAAGGGAGTGCTGAATATCAAGAGTGTACATATCATCCAAAAGAGATGT990.10643330179754022No Hit
CACCATGGGATATGGTCTTGCAACAGTTTTAAAGCCTCTGCATAATTGATAA980.1053582179409994No Hit
TGTGAGGGGAATACCAGACATTCCCCTCTTTTGGCTAATATGAGTGACTGCT970.10428313408445859No Hit
CATTAAGGGAGATCAGTATTATTTTTCTCAGGTGATGCACTGGAATCTCAGC960.10320805022791778No Hit
ACGCTGGGGATCTATTATAATGTAAGCCAATTTCTGCTAAAATGAGTCAGCA960.10320805022791778No Hit
TCTATAGGGAACACTTGGTCACTTTAAATATTACAAGTACGCCTGGAACGCA950.10213296637137698No Hit
GTGGCCGGGCGAGACAGAATATTGAGTTCTGCTAGCAGTGTGAGAACTTAAC940.10105788251483616No Hit
ATGTTAGGGATGTCCTTCATTAGGTGAATATTCATTCACAAACTGTGATATG940.10105788251483616No Hit
CCTTTGGGGCCGATCTTTGAAGGGAGGGATGCAGGGATGGTTCGTCATACAC940.10105788251483616No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)