FastQCFastQC Report
Sat 31 Dec 2022
SRR5846513.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846513.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90610
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGC62856.936320494426663No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGCCGTCT49445.4563513960931465RNA PCR Primer, Index 30 (95% over 22bp)
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCG15381.6973843946584262No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGA10431.1510870764816246No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTCTTATAC7500.8277232093587904No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATG6310.6963911268071956No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTCT6240.6886657101865136No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGAACTCGTATGC5570.6147224368171283No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGAT5360.5915461869550822No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGCC4200.4635249972409226No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGT3690.40723981900452494No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGT3050.33660743847257474No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGACCTCGTATGC2060.22734797483721444No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTC1920.21189714159585035No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTT1900.20968987970422692No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTC1820.20086083213773312No Hit
GCTTATGGGATAGGATGGTCAACGTGTAACAAGACTGTGCTCAGAAAACGAA1690.18651362984218078No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGAACTCGTATGCCGTCT1650.1820991060589339No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCTGTCTC1600.17658095132987528No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTC1470.16223374903432292No Hit
CTATGCGGGGAAACCCAGTGTGGAGAAGGCACATGTCTGTGGCTCTGAGCTG1320.14567928484714712No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGACCTCGTATGCCGTCT1290.14236839200971196No Hit
GATAAAGGGAGTGCTGAATATCAAGAGTGTACATATCATCCAAAAGAGATGT1290.14236839200971196No Hit
ATAAGCGGGCCTGAATTCAGAGCCCTTCAAGCTCAGGAAACCACGCTTCACT1270.1401611301180885No Hit
ATCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCG1220.13464297538902992No Hit
ATTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATC1190.13133208255159476No Hit
CGCTACGGGGCGTATGACAGTATGCAAAAATGAAGACTATTGTGTAAAATCA1180.13022845160578303No Hit
CCCAAACCTAGAGAAAGATGTCAGCATTGAAGTACAAGAAGGTTATAGAACT1180.13022845160578303No Hit
ATCTGAAGACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCAC1140.12581392782253614No Hit
CACCATGGGATATGGTCTTGCAACAGTTTTAAAGCCTCTGCATAATTGATAA1130.12471029687672443No Hit
CTTAAAGGGATTTTTGATAACTGTGTACTTCTGGTGACTGTACAGTTTGAAA1120.12360666593091271No Hit
GCGAGTGGGGAGGAAAATGAAGCTCGGGCTGGTTAACTGACTTGCTCAGCGT1120.12360666593091271No Hit
CAAAGAGGGGTACCTAATAAGAGATATATGAATGGAAAAACAGTTTTTGCTC1120.12360666593091271No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTAT1110.122503034985101No Hit
CCAATGGGATGTGGTTCTCTAATTCTCAAGTTTTCTCAGATAAGCATATTCT1090.12029577309347753No Hit
CAGTACGGGAATCACACATCTGAGAAATTACTTTCACAAAACGCTCTACAAA1090.12029577309347753No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGAGCTCGTATGC1090.12029577309347753No Hit
TTTGACAGGGCGATCTAGCAAAGGGAGAAGGAGATGATCAAGAAGGTGAAAA1080.11919214214766581No Hit
ATGTTAGGGATGTCCTTCATTAGGTGAATATTCATTCACAAACTGTGATATG1050.11588124931023065No Hit
ATTTTTGGGGAGATATCTTCTCACTCTGATTAAAATGGCTTATATCCAGAAA1040.11477761836441894No Hit
CAATCTGGGGGACCCACTGATCATTCAGAAGCATTTTGTTTAATTTCCATGT1030.11367398741860722No Hit
CTTTCAGGGAATCTATTTTTAAAGCAAGCTTTCCACATGAGTTACATTCAAC1020.1125703564727955No Hit
CAAACGGGGAAGCTTCAATAAGGATTGTGTGTAGGTGAATAAAGACAGCTAA1020.1125703564727955No Hit
AGCTTAGGGGATTCTCAGCCTGGTAGATGTTGGTGTATAGCACTGCTACTGA1020.1125703564727955No Hit
GTGGCCGGGCGAGACAGAATATTGAGTTCTGCTAGCAGTGTGAGAACTTAAC1020.1125703564727955No Hit
TGGTCAGGGACTATAGGTCATGCCTCTTAAACGCAGAAATGGTCTAACATTT1010.11146672552698378No Hit
CTCGACGGGCATGGGAGGCCAGGGTGTGTCCTGAGGGGAGGGTGGGGCAGGA1010.11146672552698378No Hit
ATTACTGGGGCTTAGATTTAGGATCTACATATTCTGGGCATTGAATAGGCAG1000.11036309458117206No Hit
TAACTAGGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATG1000.11036309458117206No Hit
GCATCTGGGAGTCGAGGTGAGACTAGCATCGAAAGGCACATCAACAAGAAGG980.10815583268954862No Hit
GGCCATGGGTAATACAAAATTGTTGTTTATTTGTTTGTTTTTGAGACAGAGT980.10815583268954862No Hit
CACACTGGGTCAAGTGGGTTTTATCCCAGTGATGCAGTGATGGTTTAACATA970.1070522017437369No Hit
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTAT960.10594857079792518No Hit
GCAGTGCGGGTAAGCTTCATAGTCGAGAGGGAAACAGCCCAGACCACCGGTT960.10594857079792518No Hit
TTTCTTGGGCTATTCTTCAACCACCTCGGTTACTCTGACATAGGAATTTACT960.10594857079792518No Hit
CCAACTTAAAATTTGAGCTACACAGTTTCAGAAAATGAAAACTGATATTCCA950.10484493985211346No Hit
TCTTCGGGCTCGGGAGTGAAGCTGCAGACCTTTGTGAAGGTGAGTGTTACAT950.10484493985211346No Hit
GACTTAGGGTATTTATATTGATGACTTACTCCTTTTTTGTTGAATTGTACTT940.10374130890630175No Hit
TTCTTTGGGAGAAACATTTAATGCCATACATTTTTCTCTAAGCAGTTGTTTA940.10374130890630175No Hit
TCTATAGGGAACACTTGGTCACTTTAAATATTACAAGTACGCCTGGAACGCA940.10374130890630175No Hit
GCGCGGGATCTAATACGGGGACCTACTGAGATTATTTGTAAGAATTGGAGCA940.10374130890630175No Hit
GTATTGCGGGAAAGATAACAGTTGTTTATCCCATAGATTTGGAACATGCGTT930.10263767796049No Hit
GAATTTGGGAGGCAGAAGGATCACTTGAGCCCAGGATTTCGAGGTTACAGTG920.10153404701467829No Hit
AAGTCAGGGACCGCTTTTAATCTAGACTCCTCCTGGATCAGATTATAGGACT920.10153404701467829No Hit
CAATTAGGGTCGTCCTGGGCGTGGAGCCAGAGGAGGCACCCGGGGAGGCCGG920.10153404701467829No Hit
GCAACAGGGTTACAGCCATGCCTAACTGACTTCTTGAGGTAAGATTGTTCTG920.10153404701467829No Hit
GCTACCCAGACCTCTAAAACTCTCTTCATTCAACACTAGGCTGTTTTGCTTT910.10043041606886656No Hit
CTCACAGGGCAACAAAAAGTTGTTTAAAAAAAAGATTAAAAATTTGATAACT910.10043041606886656No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)