FastQCFastQC Report
Sat 31 Dec 2022
SRR5846512.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846512.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85288
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGC52936.206031329143608No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGCCGTCT38824.551636807053748RNA PCR Primer, Index 30 (95% over 22bp)
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCG14741.7282618891285997No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGA10311.2088453240784165No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTCT6030.7070162273707907No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTCTTATAC5880.6894287590282338No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATG4310.5053465903761373No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGAT4140.48541412625457275No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGAACTCGTATGC3460.40568426976831445No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGT3270.3834068098677422No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGCC3170.37168183097270424No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGACCTCGTATGC2630.3083669449394991No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTT2040.239189569458775No Hit
CTAACTGGGATTGGGGTCGAGTGTTCATGCTTTTCATAGAGGATTTCATTTA1640.192289653878623No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTC1590.18642716443110402No Hit
AGTTCTGGGATTTGCTGGCTGTAGTGTTAGCTAAGCAATGACAAGAACTTTC1560.18290967076259262No Hit
ACTGCTGGGATTACAGACTCGAGAACAGGAAGAACTGGAAGAAGCTTTAGAA1530.17939217709408123No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTC1500.17587468342556983No Hit
TTCTGCGGCAAAAAAGGAAAGATATTCCATGTTCATAGATTGGGAGAATCAA1460.17118469186755464No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCTGTCTC1450.17001219397805084No Hit
ATCTGAAGACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCAC1400.16414970453053185No Hit
ACATGAGGGTACCTGTATGTATTTGCATAGCCAGCTGTTCCTCTCATTCAAA1370.16063221086202045No Hit
TTGTTTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1350.15828721508301286No Hit
CAACTCGGGGGAAAAGTTGAAAGTGTTTCCCCAAGATTTGGAACAAGACAAG1320.15476972141450143No Hit
ACAGTTGGGTCATCTTCAGCAAATGTTGTTTCATCTGTTTTTGATCCTTGGC1310.15359722352499766No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGACCTCGTATGCCGTCT1300.15242472563549386No Hit
TACAAAGGGAGACAAGGCTGAAATTAAGACAGTAATCAAGAATTTGCCAAGA1260.14773473407747867No Hit
TTGTAAGGGGTCCTTCCAGAGCTTTTCTGAAGAGAGATGACTATTTCATTTT1250.14656223618797487No Hit
TCTTCAGGGAGTTTTAGCCAGTTAGTAAATGGGAATACTCAGTTTTAGGGAT1240.14538973829847104No Hit
GAAACGGGAACGCTTTAGGTGACAACTGACATTTTGGTGAATTAATTAGGTG1230.14421724040896725No Hit
ACCAATGGGATTTCTAATGAAATATATTGTTTTGCCCATGTCAGGTTTAATA1230.14421724040896725No Hit
TACCTAGGGGCTTCTGCAACAGGCGTGGGTCACGCTCTCGCTCGGTCTTTCT1220.14304474251946345No Hit
CTCTCTCAGGGGCAGTTCAAGTAGTTGGACTAAAATTTCTAACAAACATGAC1220.14304474251946345No Hit
GCGTATGGGAGGGACTGCCTTGGCTGCCAGGGCAAGGTGCTCTAGGAGGTCT1220.14304474251946345No Hit
CCATCAGGGGATGGATTTTACCATCTAGACCTCTCTGGCTAATAGCTAGTCT1210.14187224462995968No Hit
TGCAAAGGGAGTAGTCTTTGTTATTAAAGGAACCTGCTGATAAGTACAAGTG1210.14187224462995968No Hit
TAGACAAGGGCATAAATTGGAGAAAATCACTATCAGTTGTATCAAAAATGTT1210.14187224462995968No Hit
CTACAAGAGGGGCCTGTTTCCCTGTAGCAGCTCCTTATTGCTGGAGAAGGAG1200.14069974674045588No Hit
ACTACAGGGAGTCTAATGGCTGATTCATCGGCATAGTTCTTGGCGTTAACAT1200.14069974674045588No Hit
TAGCACGGGTAAGAAAGACATAGTGCTTAGTTCATAGTGCTACTTAATTATG1190.13952724885095208No Hit
GCATGCGGGATAATGATGAAATTTAAAAAGCAGTATGGAGCATTATATATCA1190.13952724885095208No Hit
AAGTGGGGGTATCATGTTGAGATATGTTCCTTGTATTCCCGGTATTTTTTAA1190.13952724885095208No Hit
GTCCGGGATGGCACAAGCAGGTGTTGTCTAAGCAGTTTCTCTGTTTGCTTGT1180.13835475096144828No Hit
CACGTGGGGGCCACTGTCACCAAAACAAACAACTACCACTTTCCAAATTTCA1180.13835475096144828No Hit
CTCACAGGCCTGAGCTCAAGGGATCCACCAACTCTGGGATCCACCAACTCTG1170.13718225307194448No Hit
TAGAAAGGGCGATCTAGCTACGGGCAGAGAGGGGGGTTGCGGTTAGTGGACC1160.13600975518244068No Hit
ATCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCG1130.13249226151392926No Hit
TATCGTGGGATAGTAATTTAAGAATTGAAGTAAAAATGAAAAAGCAAAGTAG1130.13249226151392926No Hit
GGTCCAGGATGGTAATGAAACCGTGAACTTTATTGAGAACTTTCAAGCATCA1100.1289747678454179No Hit
CCGCATGGGATTACAAAAGCATGTTAATTTCAATACAGGAAAACTCTTTTGT1100.1289747678454179No Hit
GTCCTTGGGATGGTATTTGGCAGGGATAAGTATGAAATTGCTTGATTAATAA1080.1266297720664103No Hit
ATGCATGGGCTATATGAACCTGTGATTAAATAAGACATGATATGCTGAGTGA1080.1266297720664103No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTC1080.1266297720664103No Hit
TTTGTGTGGGATATCATGTGTAGATATTATGTATAGAAAATAAAATAAATTA1080.1266297720664103No Hit
CCTTATGGGGACCACAGTGAAATAAAACTATAAATCAGTAACAAGAGAAATT1080.1266297720664103No Hit
TTAACTGGGAGGCAACGCTCCAAATCTTCCGTCCTCCCGTGGATCTCCCGGG1070.12545727417690647No Hit
TTGAGGGGGCTTTTCCAAGCGGCTGCCGAAGATGGCGGAGGTGCAGGTCCTG1070.12545727417690647No Hit
GTAATTGGGTTATGTTTTCATTTTAGGACTCTTGAGAATTTCTATATACCAG1060.12428477628740268No Hit
ACTTACGGGGCCGTGGAAGAGTACAGTTGTGAATTTGGCTCCGCGAAGTATT1050.12311227839789887No Hit
TGTAACGGGGATCTACGTTAGGGGCTTCCCCTTAAGTATAACTAATTTGCTC1040.12193978050839507No Hit
ACATGGGGGAGGGCTAGGGAAGTCCTTCCCACAGCCTGCGCTTGCCTCCCTG1030.12076728261889128No Hit
AGATAAGGGGATTTTCGAATCTGGAAACTTCACGTAATTGGAACTTCTTTAT1020.1195947847293875No Hit
TAGCAAGGGAACATGAATGGAACTGGAGGTCATTATATTAAGTAAAATAAGC1020.1195947847293875No Hit
TATACAGGGATTGGCTAGCATATGGAAAGAAAACAGCTAACAGCCAAACTAA1010.11842228683988369No Hit
CAGGATGGGGCTGCAGATGTTGGGTGCAGTATTCTATGAAGACCGATTAAGT1010.11842228683988369No Hit
AGCTTAGGGTAATGGCCAAAATGTGACAGGGTTAATGTTGGAAGAGAAAAAC1010.11842228683988369No Hit
TTCCAAGGGAGATACGGTTAAAACAAAGGAAATGTTGTCAACGTTGACGCCA1010.11842228683988369No Hit
CAAGTGGGGGATATTTAAAATAATTTTATGCATTTTAAAATCTAGGTTGGAT1000.1172497889503799No Hit
GCTACCGGGCTGTATATATGACAGTGGTAATTGGACTAATTGATTGCAACAT1000.1172497889503799No Hit
CTGCAAGGGTACTTCCAATAGGAGTGGTGAGAGAAGGAATCATTGTGCTGGT1000.1172497889503799No Hit
GTTAAAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1000.1172497889503799No Hit
ATGCCTGGGGGGCGATGGGGAGGGAAGAGGAATAAGAAAAGTCACGAGGTAG1000.1172497889503799No Hit
GGATGGGGTTTTGAGTTTCAAATGAAGTTAATGGTTTCAGTGTGATTCAGTC990.1160772910608761No Hit
TAGCTCAGGGGAGTCCTCTTCAAGGAAGAGCAAGGCACAGTAAGGCAAATAA990.1160772910608761No Hit
TTTTCGGGGAAGAGGGGATAACTTATAAAAATGTGGAAACCATCTGTCCATT990.1160772910608761No Hit
CAGATAGGGATAGCACCACCTTGTGGTTAACATGCCAGTATTTTACCTTTTG980.1149047931713723No Hit
AATTAGGGATGTTTGAAGTAACCTTTTATAGAGTGAGAAAGATGTCTGTCTA970.1137322952818685No Hit
TTACAAGGGATGTTTGTGACGTTCGCCGCCATGCAGGCGCAGCAGGGCCGCA970.1137322952818685No Hit
GTGACAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA960.11255979739236469No Hit
TACGTTGGGGAAAAAAATGCAGGTGACCTAGCTGAAACAGAGAAGAGTACAG950.11138729950286089No Hit
GTTCACGGGTCTACTGAAAATTGGCCAGGAGACAGATAAAACTACAACAAGA950.11138729950286089No Hit
TAGCTGGGGCTCTTCCACAGGAGGCCTACACGCCGCCGCTTGTGCTGCAGCC940.11021480161335709No Hit
AATGCAGGGGAAATAAACTTTGGAGTATGTTCATTTAAAGAGTTTTTACATA940.11021480161335709No Hit
CGTATAGGGAGACTTAACCTAAAATGACATTTATAAGTTTTAAATATGCAGA930.10904230372385329No Hit
GTAAGGGGGAACAGCAAGAGGAAAATGATGAAGTATATAGCCAAGAGAAAAA930.10904230372385329No Hit
TTTATTGGGGTTACTTCTATTGTTTCACTTCTCTTTCTTCCCAGAAGCAGAC930.10904230372385329No Hit
ATTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATC930.10904230372385329No Hit
CTATGTGGGATGTTCTAGGTAATTACATGTGATTGCTCTCTATTTTACACAG930.10904230372385329No Hit
CCTGCAGGGAAGGTGGTTTATTACAAATAAGCACACTAACCCCTCGGAAGTT930.10904230372385329No Hit
AGATAAGGGGGGCTTGACTTTTTGTACAAATTTTAACTGTAAAATACAGATT930.10904230372385329No Hit
ACTACTGGGTTACAGAAGACATCCCCGTGAAGACTTTGAATATGAAGACTGT920.1078698058343495No Hit
TTGTGAGGGAGCCGGGGCAGCCAGCAGTACCGGGCGCTCACGGTGCCCGAGC920.1078698058343495No Hit
CCTTTGTGGGGATCTGTTACCGGGGCTCTTTTTCCGGCTGGAACCATGGAGG910.1066973079448457No Hit
AACAAAGGGCATATTATATGCATCATTAACTGAAATGTCGTTGTGCAGCATA910.1066973079448457No Hit
ATTGTTGGGGCCGGGCCACCGGAAGAAGATCGCGGCAGAGATCAGCGGCCTA910.1066973079448457No Hit
CTTAAGCGGGAATGTAATAAAATATATTTTAAAAACATGAGTATGGGAGCTT910.1066973079448457No Hit
GGTACTGGGATATTATCGGCACTTGACTTTTAAGTCTCTGTCACGTATATCA910.1066973079448457No Hit
CAAATGGGGATCTGAGACTGGGCAGGAGGCTGAGGCAGGAGAATGGCGTGAA900.10552481005534191No Hit
TAATGGCGGGCTTGGTAAACCAGGTAATTGAATGAAGAAAGGTCACTAAAGG900.10552481005534191No Hit
CTATCGTCGGGGGAGGTGAAAGACCTTTACAAGGAGAAGTACAAAACATTGA880.1031798142763343No Hit
CCCACCTCAACCTCCTGAGTAGCTGCAATCACAGGCAAACACCACCATGCCT880.1031798142763343No Hit
TCTCTTGGGTCCGATCTTTTTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGC880.1031798142763343No Hit
GAATAAGGGGAGATATCAAGATTTATGTCTGGGAACTAAAATATATAATGCC880.1031798142763343No Hit
TAGTAGGGATTCAGAGAGTTGTGCATACACCACTACTATCAATTTTAGAACA880.1031798142763343No Hit
CCAAATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA870.1020073163868305No Hit
ATTCAGGGGACTAATGATTCCGTTCCTCCCAAATTATGAAAAAGAAAAAGAT870.1020073163868305No Hit
TTAGGAGGGTGTGCTTGGTCTGTTTGACTTTACGCCCATCTCAGGACACTTC870.1020073163868305No Hit
TTCTCTGTGGCAGAATGGAGTTCCCGCTTGTAAATAAATATAAAAGTTTTAG870.1020073163868305No Hit
CTCTAGGGCAGGTCTTTTCTTCTTCTTCTTCTTCCTTTTTTTCCTTAGAGAA870.1020073163868305No Hit
TATGCAGGGGTCACAGTTATTGATAAACCATTATGAGCCTGAAGGACATTTT870.1020073163868305No Hit
ATGTCAGGGATCCAAGATCAATTCCCTCTCTCAGCACAGCCTGGGGAGGGGG860.1008348184973267No Hit
GAAAAAGGGATATGATTTGATTTACACCCTCCATTTTGTTATGTGGTATATC860.1008348184973267No Hit
TTCAGCGGGGGTACCATCGGAAAGCTGTGTGAGAGGCACTCTCACTCACTTA860.1008348184973267No Hit
GCGACTGGGCAGAATCTATGCCAGAGTCACACTGTGTGAGTGTTGACAGTGC860.1008348184973267No Hit
TGTGTGGGGCCTTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGG860.1008348184973267No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)