FastQCFastQC Report
Sat 31 Dec 2022
SRR5846511.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846511.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84846
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGC51596.080428069679184No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGCCGTCT37644.4362727765598855RNA PCR Primer, Index 30 (95% over 22bp)
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCG15051.7738019470570208No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGA10311.215142729179926No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTCT6210.7319142917756877No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTCTTATAC5780.6812342361454871No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGAT4320.5091577681917827No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATG4040.47615680173490793No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGAACTCGTATGC3790.4466916531126983No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGCC3350.3948329915376093No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGT3000.3535817834665158No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTT2060.2427928246470075No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGACCTCGTATGC1830.21568488791457466No Hit
ACATGAGGGTACCTGTATGTATTTGCATAGCCAGCTGTTCCTCTCATTCAAA1690.19918440468613724No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTC1650.19446998090658368No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTC1640.1932913749616953No Hit
TTGTAAGGGGTCCTTCCAGAGCTTTTCTGAAGAGAGATGACTATTTCATTTT1510.1779694976781463No Hit
AGTTCTGGGATTTGCTGGCTGTAGTGTTAGCTAAGCAATGACAAGAACTTTC1480.17443367984348113No Hit
TCTTCAGGGAGTTTTAGCCAGTTAGTAAATGGGAATACTCAGTTTTAGGGAT1460.17207646795370438No Hit
TTCTGCGGCAAAAAAGGAAAGATATTCCATGTTCATAGATTGGGAGAATCAA1450.170897862008816No Hit
TAGAAAGGGCGATCTAGCTACGGGCAGAGAGGGGGGTTGCGGTTAGTGGACC1430.1685406501190392No Hit
TAGACAAGGGCATAAATTGGAGAAAATCACTATCAGTTGTATCAAAAATGTT1390.16382622633948565No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCTGTCTC1370.16146901444970888No Hit
TTGTTTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1370.16146901444970888No Hit
CTAACTGGGATTGGGGTCGAGTGTTCATGCTTTTCATAGAGGATTTCATTTA1350.15911180255993213No Hit
CCATCAGGGGATGGATTTTACCATCTAGACCTCTCTGGCTAATAGCTAGTCT1350.15911180255993213No Hit
TTTGTGTGGGATATCATGTGTAGATATTATGTATAGAAAATAAAATAAATTA1310.15439737878037857No Hit
ACTGCTGGGATTACAGACTCGAGAACAGGAAGAACTGGAAGAAGCTTTAGAA1300.15321877283549018No Hit
GAAAAAGGGATATGATTTGATTTACACCCTCCATTTTGTTATGTGGTATATC1290.1520401668906018No Hit
CTCTCTCAGGGGCAGTTCAAGTAGTTGGACTAAAATTTCTAACAAACATGAC1280.1508615609457134No Hit
TATCGTGGGATAGTAATTTAAGAATTGAAGTAAAAATGAAAAAGCAAAGTAG1280.1508615609457134No Hit
ACAGTTGGGTCATCTTCAGCAAATGTTGTTTCATCTGTTTTTGATCCTTGGC1270.149682955000825No Hit
TACCTAGGGGCTTCTGCAACAGGCGTGGGTCACGCTCTCGCTCGGTCTTTCT1250.14732574311104826No Hit
CAACTCGGGGGAAAAGTTGAAAGTGTTTCCCCAAGATTTGGAACAAGACAAG1250.14732574311104826No Hit
AGATAAGGGGATTTTCGAATCTGGAAACTTCACGTAATTGGAACTTCTTTAT1230.14496853122127146No Hit
ATCTGAAGACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCAC1220.1437899252763831No Hit
CTCACAGGCCTGAGCTCAAGGGATCCACCAACTCTGGGATCCACCAACTCTG1210.1426113193314947No Hit
GTCCGGGATGGCACAAGCAGGTGTTGTCTAAGCAGTTTCTCTGTTTGCTTGT1180.13907550149682954No Hit
TATACAGGGATTGGCTAGCATATGGAAAGAAAACAGCTAACAGCCAAACTAA1180.13907550149682954No Hit
CCTTATGGGGACCACAGTGAAATAAAACTATAAATCAGTAACAAGAGAAATT1180.13907550149682954No Hit
TACAAAGGGAGACAAGGCTGAAATTAAGACAGTAATCAAGAATTTGCCAAGA1170.13789689555194115No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTC1170.13789689555194115No Hit
CCGCATGGGATTACAAAAGCATGTTAATTTCAATACAGGAAAACTCTTTTGT1170.13789689555194115No Hit
GCATGCGGGATAATGATGAAATTTAAAAAGCAGTATGGAGCATTATATATCA1160.1367182896070528No Hit
TTCAGCGGGGGTACCATCGGAAAGCTGTGTGAGAGGCACTCTCACTCACTTA1150.1355396836621644No Hit
CAGATAGGGATAGCACCACCTTGTGGTTAACATGCCAGTATTTTACCTTTTG1150.1355396836621644No Hit
TTCCAAGGGAGATACGGTTAAAACAAAGGAAATGTTGTCAACGTTGACGCCA1150.1355396836621644No Hit
GAATAAGGGGAGATATCAAGATTTATGTCTGGGAACTAAAATATATAATGCC1140.134361077717276No Hit
ACTTACGGGGCCGTGGAAGAGTACAGTTGTGAATTTGGCTCCGCGAAGTATT1140.134361077717276No Hit
CTTTATGGGGCAATAACTCTGGGAGGGGCTCGAGAGGGCTGGTCCTTATTTA1120.13200386582749923No Hit
GGATGGGGTTTTGAGTTTCAAATGAAGTTAATGGTTTCAGTGTGATTCAGTC1110.13082525988261084No Hit
CTACAAGAGGGGCCTGTTTCCCTGTAGCAGCTCCTTATTGCTGGAGAAGGAG1100.12964665393772246No Hit
TAGCAAGGGAACATGAATGGAACTGGAGGTCATTATATTAAGTAAAATAAGC1100.12964665393772246No Hit
TATACAGGGATTGTCTGTGAATTTCAGCATCAATCTGAAAAACATGTTTTGA1100.12964665393772246No Hit
GTCCTTGGGATGGTATTTGGCAGGGATAAGTATGAAATTGCTTGATTAATAA1090.1284680479928341No Hit
TTCTGCGGGACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCT1080.12728944204794568No Hit
TAGCACGGGTAAGAAAGACATAGTGCTTAGTTCATAGTGCTACTTAATTATG1070.1261108361030573No Hit
ACCAATGGGATTTCTAATGAAATATATTGTTTTGCCCATGTCAGGTTTAATA1070.1261108361030573No Hit
AATGCAGGGGAAATAAACTTTGGAGTATGTTCATTTAAAGAGTTTTTACATA1070.1261108361030573No Hit
TACGTTGGGGAAAAAAATGCAGGTGACCTAGCTGAAACAGAGAAGAGTACAG1060.12493223015816893No Hit
TGCAAAGGGAGTAGTCTTTGTTATTAAAGGAACCTGCTGATAAGTACAAGTG1050.12375362421328054No Hit
GAAACGGGAACGCTTTAGGTGACAACTGACATTTTGGTGAATTAATTAGGTG1050.12375362421328054No Hit
TTGAGGGGGCTTTTCCAAGCGGCTGCCGAAGATGGCGGAGGTGCAGGTCCTG1050.12375362421328054No Hit
ACATGGGGGAGGGCTAGGGAAGTCCTTCCCACAGCCTGCGCTTGCCTCCCTG1040.12257501826839215No Hit
TTGTGAGGGAGCCGGGGCAGCCAGCAGTACCGGGCGCTCACGGTGCCCGAGC1040.12257501826839215No Hit
AGTTTGGGGCAAGCCACAGGCCTTATTTCATATGTTTCCTCAACTGTACAAT1030.12139641232350375No Hit
TTAACTGGGAGGCAACGCTCCAAATCTTCCGTCCTCCCGTGGATCTCCCGGG1030.12139641232350375No Hit
GTTAAGGGGAAGATGTGCTGATTAACATATTCTGTGATATGGTTTACAACTT1030.12139641232350375No Hit
ATGCCTGGGGGGCGATGGGGAGGGAAGAGGAATAAGAAAAGTCACGAGGTAG1030.12139641232350375No Hit
GTAATTGGGTTATGTTTTCATTTTAGGACTCTTGAGAATTTCTATATACCAG1020.12021780637861539No Hit
GCGTATGGGAGGGACTGCCTTGGCTGCCAGGGCAAGGTGCTCTAGGAGGTCT1020.12021780637861539No Hit
TCTCTTGGGTCCGATCTTTTTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGC1020.12021780637861539No Hit
GGTCTAGGGAAGACATTTACCTATTTGTCTTTCCTTACGTTCTCAAAATATT1020.12021780637861539No Hit
ACTACAGGGAGTCTAATGGCTGATTCATCGGCATAGTTCTTGGCGTTAACAT1020.12021780637861539No Hit
AAGTGGGGGTATCATGTTGAGATATGTTCCTTGTATTCCCGGTATTTTTTAA1010.11903920043372698No Hit
TTGGCTGGGATACGAAATTGCAAATCTAAGTGAAAAACCTTTGTGTAAAACA1000.1178605944888386No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGACCTCGTATGCCGTCT1000.1178605944888386No Hit
GTAAGGGGGAACAGCAAGAGGAAAATGATGAAGTATATAGCCAAGAGAAAAA990.11668198854395022No Hit
CTATCGTCGGGGGAGGTGAAAGACCTTTACAAGGAGAAGTACAAAACATTGA990.11668198854395022No Hit
GGCCATGGGGATGGGAGTGAAAACAACAGCTTTGTCCAGTAGCTTTAGCTGG990.11668198854395022No Hit
TTTAACCAATTAAAATCCCTTTTGTTGATGAGCTATTGAGAGCTGCAGTAGT990.11668198854395022No Hit
GCGACTGGGCAGAATCTATGCCAGAGTCACACTGTGTGAGTGTTGACAGTGC990.11668198854395022No Hit
GATTTGGGAGTATAAGGTAGGCAGAGATAAGATGGGAAAGGAATTTGAATTA990.11668198854395022No Hit
AACAAAGGGCATATTATATGCATCATTAACTGAAATGTCGTTGTGCAGCATA980.11550338259906183No Hit
TTTTCGGGGAAGAGGGGATAACTTATAAAAATGTGGAAACCATCTGTCCATT980.11550338259906183No Hit
TAGCTGGGGCTCTTCCACAGGAGGCCTACACGCCGCCGCTTGTGCTGCAGCC970.11432477665417344No Hit
ATGTCAGGGATCCAAGATCAATTCCCTCTCTCAGCACAGCCTGGGGAGGGGG960.11314617070928505No Hit
CGTATAGGGAGACTTAACCTAAAATGACATTTATAAGTTTTAAATATGCAGA960.11314617070928505No Hit
AGCTTAGGGTAATGGCCAAAATGTGACAGGGTTAATGTTGGAAGAGAAAAAC960.11314617070928505No Hit
ATTCAGGGGACTAATGATTCCGTTCCTCCCAAATTATGAAAAAGAAAAAGAT950.11196756476439668No Hit
TAGCTCAGGGGAGTCCTCTTCAAGGAAGAGCAAGGCACAGTAAGGCAAATAA950.11196756476439668No Hit
TGTGTGGGGCCTTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGG950.11196756476439668No Hit
AGCCTAGGGATGTCTGTCAAGTGAAGCACATTCATACCTTGACTGGACTAAA940.11078895881950829No Hit
TTACAAGGGATGTTTGTGACGTTCGCCGCCATGCAGGCGCAGCAGGGCCGCA940.11078895881950829No Hit
TGTAACGGGGATCTACGTTAGGGGCTTCCCCTTAAGTATAACTAATTTGCTC940.11078895881950829No Hit
GGTCCAGGATGGTAATGAAACCGTGAACTTTATTGAGAACTTTCAAGCATCA930.1096103528746199No Hit
ATGCATGGGCTATATGAACCTGTGATTAAATAAGACATGATATGCTGAGTGA930.1096103528746199No Hit
GTGACAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA920.10843174692973152No Hit
CGCTCGGGGTGGCACAGCCTAAAAAAGGAGTAATGTGAAATTAAATTATTTT920.10843174692973152No Hit
GTTAAAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC920.10843174692973152No Hit
TGTGCTGGGATTACAGGTGTGAGCCACCGTGCCTGGCCTCATGGGTTTTCTT910.10725314098484313No Hit
CCTTTGTGGGGATCTGTTACCGGGGCTCTTTTTCCGGCTGGAACCATGGAGG910.10725314098484313No Hit
ACTACTGGGTTACAGAAGACATCCCCGTGAAGACTTTGAATATGAAGACTGT910.10725314098484313No Hit
GTTCACGGGTCTACTGAAAATTGGCCAGGAGACAGATAAAACTACAACAAGA910.10725314098484313No Hit
TAATGGCGGGCTTGGTAAACCAGGTAATTGAATGAAGAAAGGTCACTAAAGG910.10725314098484313No Hit
CTTAAGCGGGAATGTAATAAAATATATTTTAAAAACATGAGTATGGGAGCTT900.10607453503995473No Hit
AATTAGGGATGTTTGAAGTAACCTTTTATAGAGTGAGAAAGATGTCTGTCTA900.10607453503995473No Hit
CACGTGGGGGCCACTGTCACCAAAACAAACAACTACCACTTTCCAAATTTCA900.10607453503995473No Hit
GCTTCAGGGATTTCTGAATTTTCTTTTCTGCATCTGTGTCTGCCCCTTCTTT900.10607453503995473No Hit
CTAGAGGGTAGCAAACTGAATTCAACAATACATTAAAAATATCATTCATCAT900.10607453503995473No Hit
CTATGTGGGATGTTCTAGGTAATTACATGTGATTGCTCTCTATTTTACACAG890.10489592909506637No Hit
CTGCAAGGGTACTTCCAATAGGAGTGGTGAGAGAAGGAATCATTGTGCTGGT890.10489592909506637No Hit
TGAATCGGGGGAGGAGGGATTCCCTGACTTTGACACACATGGCCACCTTGGC880.10371732315017797No Hit
CTCGCAGGGCAAGAGCAAAGTTTCCGTTGATGAAACAGACATCCCACAACAA880.10371732315017797No Hit
GGTACTGGGATATTATCGGCACTTGACTTTTAAGTCTCTGTCACGTATATCA880.10371732315017797No Hit
ATGTGTGGGATGGCTTGGCTTTTATGTTTGAGGTTTTCCCATTTTGGTCCAT880.10371732315017797No Hit
GCTACCGGGCTGTATATATGACAGTGGTAATTGGACTAATTGATTGCAACAT870.10253871720528958No Hit
GACTCCAGTGGCTGCAGCCTATCCCAGAAGCTCAGGGTGCAAGGCAAGAGGT870.10253871720528958No Hit
ACCCATGGAAGAGATTTATCATGGCCACGAATTAGAAGACTTAATATTGTTA870.10253871720528958No Hit
TAGTAGGGATTCAGAGAGTTGTGCATACACCACTACTATCAATTTTAGAACA870.10253871720528958No Hit
TATCGTGGGCACTGGAATTCAACTATTTTATGTCATTCTCTTTCTCACGCTT870.10253871720528958No Hit
TCAGCTCGGGAAGCTGGGTTGTCTTTAAACAACCCTAAATACACGTCTGTTT860.10136011126040119No Hit
CAGGATGGGGCTGCAGATGTTGGGTGCAGTATTCTATGAAGACCGATTAAGT860.10136011126040119No Hit
ATCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCG850.10018150531551281No Hit
ATAATGGGGGAATCTCTGAAAACAAATGCTTTTACATTTTAATTTAAAAAGA850.10018150531551281No Hit
AACTGTGGGATATTGTAAGGAGTTATTTACCCAAAACACTTGCTCCAATTTT850.10018150531551281No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)