FastQCFastQC Report
Sat 31 Dec 2022
SRR5846510.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846510.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83715
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGC49555.918891477035179No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGCCGTCT37844.520097951382667RNA PCR Primer, Index 30 (95% over 22bp)
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCG13731.640088395150212No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGA9941.1873618825777936No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTCTTATAC5510.6581855103625396No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTCT5460.652212865077943No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGAACTCGTATGC4500.5375380756136893No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGAT4330.517231081646061No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATG4050.4837842680523204No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGT3340.3989727050110494No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGCC3300.3941945887833721No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTT2020.24129486949770054No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTC1630.19470823627784745No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCTGTCTC1630.19470823627784745No Hit
CTAACTGGGATTGGGGTCGAGTGTTCATGCTTTTCATAGAGGATTTCATTTA1620.19351370722092814No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGACCTCGTATGC1610.19231917816400884No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTC1510.18037388759481573No Hit
CTCTCTCAGGGGCAGTTCAAGTAGTTGGACTAAAATTTCTAACAAACATGAC1510.18037388759481573No Hit
ACATGAGGGTACCTGTATGTATTTGCATAGCCAGCTGTTCCTCTCATTCAAA1450.1732067132532999No Hit
TTCTGCGGCAAAAAAGGAAAGATATTCCATGTTCATAGATTGGGAGAATCAA1420.16962312608254196No Hit
AGTTCTGGGATTTGCTGGCTGTAGTGTTAGCTAAGCAATGACAAGAACTTTC1370.16365048079794542No Hit
TATCGTGGGATAGTAATTTAAGAATTGAAGTAAAAATGAAAAAGCAAAGTAG1370.16365048079794542No Hit
GATTTGGGAGTATAAGGTAGGCAGAGATAAGATGGGAAAGGAATTTGAATTA1360.1624559517410261No Hit
ATCTGAAGACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCAC1360.1624559517410261No Hit
TACCTAGGGGCTTCTGCAACAGGCGTGGGTCACGCTCTCGCTCGGTCTTTCT1330.15887236457026815No Hit
GGATGGGGTTTTGAGTTTCAAATGAAGTTAATGGTTTCAGTGTGATTCAGTC1300.15528877739951025No Hit
TAGACAAGGGCATAAATTGGAGAAAATCACTATCAGTTGTATCAAAAATGTT1300.15528877739951025No Hit
AGATAAGGGGATTTTCGAATCTGGAAACTTCACGTAATTGGAACTTCTTTAT1270.1517051902287523No Hit
CCATCAGGGGATGGATTTTACCATCTAGACCTCTCTGGCTAATAGCTAGTCT1270.1517051902287523No Hit
GTCCTTGGGATGGTATTTGGCAGGGATAAGTATGAAATTGCTTGATTAATAA1270.1517051902287523No Hit
GTCCGGGATGGCACAAGCAGGTGTTGTCTAAGCAGTTTCTCTGTTTGCTTGT1220.14573254494415577No Hit
TTGTAAGGGGTCCTTCCAGAGCTTTTCTGAAGAGAGATGACTATTTCATTTT1220.14573254494415577No Hit
CTACAAGAGGGGCCTGTTTCCCTGTAGCAGCTCCTTATTGCTGGAGAAGGAG1210.14453801588723644No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTC1210.14453801588723644No Hit
ACTGCTGGGATTACAGACTCGAGAACAGGAAGAACTGGAAGAAGCTTTAGAA1200.14334348683031714No Hit
TAGAAAGGGCGATCTAGCTACGGGCAGAGAGGGGGGTTGCGGTTAGTGGACC1200.14334348683031714No Hit
TTGTTTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1190.14214895777339784No Hit
CCTTATGGGGACCACAGTGAAATAAAACTATAAATCAGTAACAAGAGAAATT1190.14214895777339784No Hit
CAACTCGGGGGAAAAGTTGAAAGTGTTTCCCCAAGATTTGGAACAAGACAAG1180.1409544287164785No Hit
TAGCAAGGGAACATGAATGGAACTGGAGGTCATTATATTAAGTAAAATAAGC1180.1409544287164785No Hit
AAGTGGGGGTATCATGTTGAGATATGTTCCTTGTATTCCCGGTATTTTTTAA1170.1397598996595592No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGACCTCGTATGCCGTCT1160.1385653706026399No Hit
CTTTATGGGGCAATAACTCTGGGAGGGGCTCGAGAGGGCTGGTCCTTATTTA1160.1385653706026399No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGAACTCGTATGCCGTCT1140.1361763124888013No Hit
TCTTCAGGGAGTTTTAGCCAGTTAGTAAATGGGAATACTCAGTTTTAGGGAT1130.134981783431882No Hit
TAGCACGGGTAAGAAAGACATAGTGCTTAGTTCATAGTGCTACTTAATTATG1120.13378725437496267No Hit
GTTAAGGGGAAGATGTGCTGATTAACATATTCTGTGATATGGTTTACAACTT1120.13378725437496267No Hit
GTAATTGGGTTATGTTTTCATTTTAGGACTCTTGAGAATTTCTATATACCAG1120.13378725437496267No Hit
GAAACGGGAACGCTTTAGGTGACAACTGACATTTTGGTGAATTAATTAGGTG1120.13378725437496267No Hit
GCTACCGGGCTGTATATATGACAGTGGTAATTGGACTAATTGATTGCAACAT1110.13259272531804336No Hit
TACGTTGGGGAAAAAAATGCAGGTGACCTAGCTGAAACAGAGAAGAGTACAG1100.13139819626112406No Hit
GTGTGGAGGGATTAGCTGTATGACTATGGATATGTCACTTCTCAGCTTCCAT1100.13139819626112406No Hit
TTTGTGTGGGATATCATGTGTAGATATTATGTATAGAAAATAAAATAAATTA1100.13139819626112406No Hit
TAGCTCAGGGGAGTCCTCTTCAAGGAAGAGCAAGGCACAGTAAGGCAAATAA1090.13020366720420473No Hit
ATGCATGGGCTATATGAACCTGTGATTAAATAAGACATGATATGCTGAGTGA1090.13020366720420473No Hit
TATACAGGGATTGTCTGTGAATTTCAGCATCAATCTGAAAAACATGTTTTGA1090.13020366720420473No Hit
GCGTATGGGAGGGACTGCCTTGGCTGCCAGGGCAAGGTGCTCTAGGAGGTCT1070.12781460909036613No Hit
ACTTACGGGGCCGTGGAAGAGTACAGTTGTGAATTTGGCTCCGCGAAGTATT1070.12781460909036613No Hit
TGTGTGGGGCCTTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGG1070.12781460909036613No Hit
ACATGGGGGAGGGCTAGGGAAGTCCTTCCCACAGCCTGCGCTTGCCTCCCTG1060.1266200800334468No Hit
GAATAAGGGGAGATATCAAGATTTATGTCTGGGAACTAAAATATATAATGCC1060.1266200800334468No Hit
CAGATAGGGATAGCACCACCTTGTGGTTAACATGCCAGTATTTTACCTTTTG1060.1266200800334468No Hit
TACAAAGGGAGACAAGGCTGAAATTAAGACAGTAATCAAGAATTTGCCAAGA1050.1254255509765275No Hit
GGTACTGGGATATTATCGGCACTTGACTTTTAAGTCTCTGTCACGTATATCA1030.12303649286268888No Hit
AATTAGGGATGTTTGAAGTAACCTTTTATAGAGTGAGAAAGATGTCTGTCTA1020.12184196380576957No Hit
TGCAAAGGGAGTAGTCTTTGTTATTAAAGGAACCTGCTGATAAGTACAAGTG1020.12184196380576957No Hit
CAAGTGGGGGATATTTAAAATAATTTTATGCATTTTAAAATCTAGGTTGGAT1010.12064743474885027No Hit
TTCAGCGGGGGTACCATCGGAAAGCTGTGTGAGAGGCACTCTCACTCACTTA1000.11945290569193096No Hit
TTTAACCAATTAAAATCCCTTTTGTTGATGAGCTATTGAGAGCTGCAGTAGT1000.11945290569193096No Hit
TTGTGAGGGAGCCGGGGCAGCCAGCAGTACCGGGCGCTCACGGTGCCCGAGC1000.11945290569193096No Hit
ACCAATGGGATTTCTAATGAAATATATTGTTTTGCCCATGTCAGGTTTAATA1000.11945290569193096No Hit
ATGCCTGGGGGGCGATGGGGAGGGAAGAGGAATAAGAAAAGTCACGAGGTAG1000.11945290569193096No Hit
GGTCCAGGATGGTAATGAAACCGTGAACTTTATTGAGAACTTTCAAGCATCA990.11825837663501165No Hit
ATAATGGGGGAATCTCTGAAAACAAATGCTTTTACATTTTAATTTAAAAAGA990.11825837663501165No Hit
CTGCAAGGGTACTTCCAATAGGAGTGGTGAGAGAAGGAATCATTGTGCTGGT990.11825837663501165No Hit
TGTAACGGGGATCTACGTTAGGGGCTTCCCCTTAAGTATAACTAATTTGCTC990.11825837663501165No Hit
GTTCCAGGGGTCTCTTCATCCAGATTCTACTTAGATTTTCAGTTGTCTTTGG980.11706384757809235No Hit
TAGCTGGGGCTCTTCCACAGGAGGCCTACACGCCGCCGCTTGTGCTGCAGCC980.11706384757809235No Hit
GGCCATGGGGATGGGAGTGAAAACAACAGCTTTGTCCAGTAGCTTTAGCTGG980.11706384757809235No Hit
ATGTGTGGGATGGCTTGGCTTTTATGTTTGAGGTTTTCCCATTTTGGTCCAT980.11706384757809235No Hit
ATGTCAGGGATCCAAGATCAATTCCCTCTCTCAGCACAGCCTGGGGAGGGGG970.11586931852117302No Hit
CCTGCAGGGAAGGTGGTTTATTACAAATAAGCACACTAACCCCTCGGAAGTT970.11586931852117302No Hit
GTTAAAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC970.11586931852117302No Hit
TTTTTGGGGCTTAAAATATATATAAAATAAAACAATTATTTGCCTTTTTTCA970.11586931852117302No Hit
TTCTGCGGGACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCT960.11467478946425372No Hit
AATATGGGAATAAAAGACCCCACTGAATGAAGCCGGAATTCTTTTAGGCAAT960.11467478946425372No Hit
TTCTCAGGGAGATGCCTTGTAAATTATGTCTTTAACGTTTTCTTATAGACTA940.11228573135041509No Hit
TGGTCAGGGACCACTTACGCGAGTAGACAGAACTCGGCTTTTCAGAAAATAG930.11109120229349578No Hit
GACTCCAGTGGCTGCAGCCTATCCCAGAAGCTCAGGGTGCAAGGCAAGAGGT930.11109120229349578No Hit
ACAGTTGGGTCATCTTCAGCAAATGTTGTTTCATCTGTTTTTGATCCTTGGC930.11109120229349578No Hit
GGTCTAGGGAAGACATTTACCTATTTGTCTTTCCTTACGTTCTCAAAATATT930.11109120229349578No Hit
ACTACAGGGAGTCTAATGGCTGATTCATCGGCATAGTTCTTGGCGTTAACAT930.11109120229349578No Hit
ACTACTGGGTTACAGAAGACATCCCCGTGAAGACTTTGAATATGAAGACTGT920.10989667323657648No Hit
ATTGTTGGGGCCGGGCCACCGGAAGAAGATCGCGGCAGAGATCAGCGGCCTA920.10989667323657648No Hit
TATACAGGGATTGGCTAGCATATGGAAAGAAAACAGCTAACAGCCAAACTAA920.10989667323657648No Hit
TTAGGAGGGTGTGCTTGGTCTGTTTGACTTTACGCCCATCTCAGGACACTTC920.10989667323657648No Hit
TTCTCTGTGGCAGAATGGAGTTCCCGCTTGTAAATAAATATAAAAGTTTTAG920.10989667323657648No Hit
GCTTCAGGGATTTCTGAATTTTCTTTTCTGCATCTGTGTCTGCCCCTTCTTT920.10989667323657648No Hit
ATATTGGGGCGCAGAAGAGGGAAGGTCTCCTTGACAAGGAATAGCGTATTTT920.10989667323657648No Hit
GAAAAAGGGATATGATTTGATTTACACCCTCCATTTTGTTATGTGGTATATC910.10870214417965716No Hit
TTAACTGGGAGGCAACGCTCCAAATCTTCCGTCCTCCCGTGGATCTCCCGGG910.10870214417965716No Hit
AGGCTTGGGAATATCACTTTCCACATAGTATAAGAACTGTACAGAACTGCCA910.10870214417965716No Hit
TAATGGCGGGCTTGGTAAACCAGGTAATTGAATGAAGAAAGGTCACTAAAGG910.10870214417965716No Hit
TTCCAAGGGAGATACGGTTAAAACAAAGGAAATGTTGTCAACGTTGACGCCA910.10870214417965716No Hit
ATCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCG900.10750761512273786No Hit
ACCCATGGAAGAGATTTATCATGGCCACGAATTAGAAGACTTAATATTGTTA900.10750761512273786No Hit
GCATGCGGGATAATGATGAAATTTAAAAAGCAGTATGGAGCATTATATATCA890.10631308606581856No Hit
TGCCAAAAACAAAAACAAAAACAAAAAACCCCACAAGAAATCCAAACCAAAA890.10631308606581856No Hit
CTATTTGGGCGATCTTAATGGCGGGTCTCGAAACGGGTGTTTGTAAACATGT890.10631308606581856No Hit
CCGCATGGGATTACAAAAGCATGTTAATTTCAATACAGGAAAACTCTTTTGT890.10631308606581856No Hit
GTTCACGGGTCTACTGAAAATTGGCCAGGAGACAGATAAAACTACAACAAGA880.10511855700889923No Hit
AAGGCGGGACCTTTATCAAGATGCTATTAAAATTGAACATCAAGTATAATAT870.10392402795197993No Hit
TTGCTGAGGGGATCTATCACCGGGCTCTTTTCCTCCTTGGCTGTCTGAAGAT870.10392402795197993No Hit
AGCTTAGGGTAATGGCCAAAATGTGACAGGGTTAATGTTGGAAGAGAAAAAC870.10392402795197993No Hit
CGTATAGGGAGACTTAACCTAAAATGACATTTATAAGTTTTAAATATGCAGA860.10272949889506063No Hit
GTAAGGGGGAACAGCAAGAGGAAAATGATGAAGTATATAGCCAAGAGAAAAA860.10272949889506063No Hit
TGCGATGGGGACAGACGTTAAATAAGAGAGAAATAAACCTTATGATCTTTAA860.10272949889506063No Hit
TTACAAGGGATGTTTGTGACGTTCGCCGCCATGCAGGCGCAGCAGGGCCGCA860.10272949889506063No Hit
CTCTTAGGGAGTGTGTGAACTAAACTTCCAAGACTTTTAATCTAGCTAATGC860.10272949889506063No Hit
AATGCAGGGGAAATAAACTTTGGAGTATGTTCATTTAAAGAGTTTTTACATA860.10272949889506063No Hit
ATCGTAGGGACCTGTAACCTCAGCTACTCAGGAGGCTGAGGCAGGAGAATCA850.10153496983814131No Hit
TTGAAGGGATGGTAGACTGTAAACTGATCCCAAATGTCTTAATGTAGATGTG850.10153496983814131No Hit
AACTGTGGGATATTGTAAGGAGTTATTTACCCAAAACACTTGCTCCAATTTT850.10153496983814131No Hit
CTCAAGGGGGAAAGAAAGTTTTCCATTAACCTCAAAATTAAAGGACTCTATA840.100340440781222No Hit
CACGTGGGGGCCACTGTCACCAAAACAAACAACTACCACTTTCCAAATTTCA840.100340440781222No Hit
CTCACAGGCCTGAGCTCAAGGGATCCACCAACTCTGGGATCCACCAACTCTG840.100340440781222No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)