FastQCFastQC Report
Sat 31 Dec 2022
SRR5846506.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846506.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159282
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGCCGTCT53293.345638553006617RNA PCR Primer, Index 30 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGC51803.2520937707964492No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCG14650.9197523888449417No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTCTTATAC8520.5349003653896862No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGA7740.4859306136286586No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTCT7150.44888939114275317No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATG6050.3798294848130988No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGAT4090.2567772880802602No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGAACTCGTATGC3770.23668713351163345No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGT3220.20215718034680633No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGCC2930.18395047776898832No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGACCTCGTATGCCGTCT2300.1443979859620045No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGACCTCGTATGC2280.14314235130146533No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCTGTCTC2220.1393754473198478No Hit
TTATAGGGAGGTGGTGGATCAATTTATTGAAGACTATTTCTGATCATCAAGC2170.1362363606684999No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTC2130.13372509134742155No Hit
AGCCTAGGGTATTTTTGATGTTTGAGATTACGCTTTCAGTTCCACACAGTCG2010.12619128338418653No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTC1880.11802965809068193No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTC1830.11489057143933401No Hit
ACTAATGGGCATAAAACCAATAACAGCCTTGATAGAAATAGTTGAAATTAAT1760.11049585012744692No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTT1700.1067289461458294No Hit
TCTATGGGGAGTTGCTTCAGCGTCCCGGTGTGGCTGTGCCGTTGGTCCTGTG1600.10045077284313356No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)