FastQCFastQC Report
Sat 31 Dec 2022
SRR5846505.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846505.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156083
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGC51523.300807903487247No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGCCGTCT51303.2867128386819835RNA PCR Primer, Index 30 (95% over 22bp)
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCG14640.9379624943139228No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTCTTATAC8230.5272835606696437No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGA7530.4824356271983496No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTCT7260.46513713857370753No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATG6270.4017093469500202No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGAT4010.2569145903141278No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGAACTCGTATGC3690.23641267787010756No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGCC3130.20053433109307228No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGT3090.19797159203756975No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTC2110.135184485177758No Hit
TTATAGGGAGGTGGTGGATCAATTTATTGAAGACTATTTCTGATCATCAAGC1980.12685558324737478No Hit
AGAGGTCTTCCAGAAGGATCTACTGGTGACGTGTGTAGAATATTGTTTTATT1700.10891640985885714No Hit
AGCCTAGGGTATTTTTGATGTTTGAGATTACGCTTTCAGTTCCACACAGTCG1690.10827572509498151No Hit
ACTAATGGGCATAAAACCAATAACAGCCTTGATAGAAATAGTTGAAATTAAT1690.10827572509498151No Hit
TGCAGCGGGATGTCTGACAAGACTAACAATTCTGTTATTAACATGACCCAAA1680.10763504033110588No Hit
ACTTCAGGGGAAGAATACTGTAAATGAAAAAACATGGATTACTGAAAGATCT1680.10763504033110588No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTT1660.10635367080335462No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCTGTCTC1660.10635367080335462No Hit
TAACGCGGGCTTCGTCTAACATCTTTATTTCAGAACGAGAAAAAGATCACAG1630.10443161651172772No Hit
TTTACCGGAAGGTAACCACTGTTCTGACTTATGTCACCATAGTTTTGTTTTG1570.10058750792847396No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)