FastQCFastQC Report
Sat 31 Dec 2022
SRR5846504.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846504.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155294
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGCCGTCT52283.3665177019073496RNA PCR Primer, Index 30 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGC49773.204888791582418No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCG14990.9652658827771838No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTCTTATAC8300.5344701018712893No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGA7840.504848867309748No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTCT7110.45784125594034536No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATG6560.4224245624428503No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGAACTCGTATGC4460.2871971872705964No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGAT3980.2562880729455098No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGCC2850.18352286630520176No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGT2770.17837134725102063No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCTGTCTC2000.12878797635452754No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGACCTCGTATGC1920.12363645730034643No Hit
AGCCTAGGGTATTTTTGATGTTTGAGATTACGCTTTCAGTTCCACACAGTCG1860.11977281800971062No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTC1850.11912887812793797No Hit
AGAGGTCTTCCAGAAGGATCTACTGGTGACGTGTGTAGAATATTGTTTTATT1780.11462129895552951No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTT1620.10431826084716732No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTC1580.10174250132007676No Hit
CCAGATGCGGGTACTTTAATTTCTCATTTAGCAAAAACAGAACAGAACAAAC1580.10174250132007676No Hit
GCAAAGGGGATAGATCCTGAATATATCTCCAGAGTACTTTGTAATTTTCAGT1580.10174250132007676No Hit
TTATAGGGAGGTGGTGGATCAATTTATTGAAGACTATTTCTGATCATCAAGC1570.10109856143830412No Hit
TTTACCGGAAGGTAACCACTGTTCTGACTTATGTCACCATAGTTTTGTTTTG1570.10109856143830412No Hit
TGTTACGGGAGTGGCCCTCAGCTTGCAGCACAGCAAATTACTTTTCTGGGCA1570.10109856143830412No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)