FastQCFastQC Report
Sat 31 Dec 2022
SRR5846503.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846503.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73224
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGC42465.798645252922539No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGCCGTCT31814.344204086091992RNA PCR Primer, Index 30 (95% over 22bp)
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCG11991.6374412760843438No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGA7521.0269856877526493No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTCTTATAC5130.7005899705014749No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTCT4980.6801048836447067No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGAT3500.4779853599912597No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGAACTCGTATGC2650.36190320113623947No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATG2520.344149459193707No Hit
ACTAGGGACACTCTGGTATTCTCCATTATTTTTTATTTCATTAAGAAAAATT2370.3236643723369387No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGT2160.2949852507374631No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGT2160.2949852507374631No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGCC2090.28542554353763794No Hit
GATGATGGGTAAAGAATCTATGTTGATCAGAAATGTTCCCTTAGATTGTAAA1940.26494045668086963No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGACCTCGTATGC1870.2553807494810445No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTT1630.22260461051021524No Hit
AATTAAGGGCGATCTTTGGCTGGGGAAGTCATGAAGTTGATGCTAATAAGCC1630.22260461051021524No Hit
CACTCAGGGAGAATTCTCACTGTGATATATAAACTCAGACCCAAACATTAAT1570.21441057576750794No Hit
GGGTTTGGGGAAGGACTTCTAGGGGCTCCGAGAGTTCCCTGAAATTAGAAAC1530.20894788593903638No Hit
TCTTAGGGGCTGGCACCTGTGTCTACTTGCTCCCGAGAAGAGGAGCGCTCAT1490.20348519611056484No Hit
GCCGGTAGGAATAGACGTAGACACACGAGCATATTTCACCTCCGCTACCATA1480.20211952365344696No Hit
TTTACCGGGCCACGAAGCTGCTGAATCACAATCTGCATTTTAATAGAATCCC1450.19802250628209328No Hit
TACACGAGGGCAGCTCTTAGTTCACAGCAGCCTGAAAAGGTGATGGCAAAGC1440.19665683382497542No Hit
CAAGCAGGGATGAGAATGTCATTTGCTAATGAATTTTAAAAGCATATATAAA1440.19665683382497542No Hit
CTATTTGGGAAATCTCAAAACAGAGCTCAGCTGCAAAAAAGCATATTTTCTG1430.19529116136785754No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCTGTCTC1380.18846279908226812No Hit
TGGCTCACTGCAACCTCCGCCGCCCGGGTTCGGGCGATTCTCCTGCCTCGGC1370.18709712662515024No Hit
TATATAGGGATCTCCTCAAGGGCTTGTTAACAAATCAGACTTTACACTATAT1360.18573145416803233No Hit
AGCGCTGGGAACTACGCAAAGGCCCCAACGTTGTAGGCCCCTACGGGCTACT1360.18573145416803233No Hit
CGTTATGGGAGTTCATCTGATTTTTACTTTGTTCCCATTTATAATTCAAATA1350.18436578171091444No Hit
AGCCTGGGTGACTGAGCGGGACTCCGTCTCAAAAAAAATAAAGAAGTAAAAA1340.18300010925379656No Hit
CAACAAGGGAGATGTAATGATTTCATTTTTATAACTATTCAAAATTTTAAGT1340.18300010925379656No Hit
TGATTTAAAATTCCCAACTGCTCCACAACATAACCTGAGACTGTTTCCTTAT1330.1816344367966787No Hit
CAGCTGGGGGGCCCGCAGCCGGTACTGCGGGACCCACTGCGGATATGGCTGT1310.1789030918824429No Hit
CATTAAGGGGGATAAAAGGAAAATAACCGTCTGCCGATGGTCCGTACTTCTT1310.1789030918824429No Hit
TTTACTGGGGGGATTAGAACAAAGACCATGCAATCACAGATTTCTTAAAAAT1300.17753741942532503No Hit
GTATATGGGGTCTCTCAGTGCCAAGTTGTCAAAAACATCTCTATTTTGCTTA1270.17344040205397138No Hit
TAATCGGGGGTCAGAAGTTGGGGTCTCCGTGGGCATTGTGATCCGTCCCAGG1250.1707090571397356No Hit
GCCAAGGGGCTTGTTAACAAATCAGACTTTACACTATATACAGATGTGACAG1250.1707090571397356No Hit
ACACGGGAAATGCCATGGCAACATCAGGAAGTTACCCATATGGTCTACAAAG1230.16797771222549984No Hit
TTTAATGGGCCCTACATTACATTTAATGCCTGTCTTAGCCCTGAAAGGTTGG1230.16797771222549984No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTC1230.16797771222549984No Hit
TGCTACGGGTATTATTAGTCTTAGATAACTTGAAAAAGACAATTATCACATC1230.16797771222549984No Hit
ACTAACGGGAACTTTCCTAATAAGGATGTTAGGTTTTCTTCTCTTTCATTAG1220.16661203976838196No Hit
TTAATAGGGGTAGAGCAATGGTTCTTAAACTTTGGTATGCACGCAAATCACC1220.16661203976838196No Hit
ATAACTGGGAGGCAAACAAGGAAAGGAAAGGAAAGAAGAAAAGGTGCCTTAG1210.16524636731126405No Hit
GCACGGGCCTATTCTACACCCTAGTAGGCTCCCTTCCCCTACTCATCGCACT1210.16524636731126405No Hit
ATCGAGCGGGGTCTAGCTGAGGGCATTCCTATTTGTTTCCATTTTGCCTATG1200.16388069485414616No Hit
AAAATAGGAAAATGTTACATCTAGAATAGTGTTAGAATTGACCAAAACTAGA1190.1625150223970283No Hit
CTTCTCGGGTGCTGAGAATGAGAGCGAACTGTACTAAAGGCCATTAAAGCAC1190.1625150223970283No Hit
ATAAGTGGGGAAATCTATCAAACATTCAAAGAAAAACTACTATAAATACTTC1190.1625150223970283No Hit
AAATAGGGACAAGGAAACATCATATACATCTCATGCAGACCTCACTGCCCTT1190.1625150223970283No Hit
GTTTCTGGGAAGATTTTGTCTTCATTTAAAACTCTGAATCTATTCCATTGAT1170.1597836774827925No Hit
ACATTAGGGAGAAATTCAAATTTTCAGCATTATTGCTAATGTATGGAAACAA1160.15841800502567463No Hit
AGCTTAGGGGTGGAAACAACTGACCTCAAGAAAGGCTGGAGGCTTCATGGCT1160.15841800502567463No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGACCTCGTATGCCGTCT1140.1556866601114389No Hit
ATTAGAGGGTGAGTTGAGCAGGATTATTCATAGGAATAAAGTAAGAAAAAAA1120.1529553151972031No Hit
CTAGCTGGGAAGTAAAGTTCTGCATGCTTGATTGAGAAAAAGACTTTTATTC1120.1529553151972031No Hit
ATGCTAGGGGGGACTTGTGAGGACTCACATAAATGCTGTTGTGGCAAAGATT1120.1529553151972031No Hit
TCTTATGGGCCACTTCACACCCATTAGGATGGCCGGACTAAAAAAGATGCAT1100.15022397028296733No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTC1090.14885829782584944No Hit
TCTGCCGGGGAATGTAAACATTAATGTTTCCTTATGGGAAAACAAATAAAGT1090.14885829782584944No Hit
CAAAGCGGTGTGCCTGGAATTTCTGTGAATGCCCTGTACATGTTGGTTCCTT1090.14885829782584944No Hit
AGCTAGGGATTCATGCATGTTATGTGTATCAATAGTTCATTCCTTTTTATTG1080.14749262536873156No Hit
CAAGCAGGGGTGTGGTGGAGTTGGACAGAATGAGATGATAGTGAAAACTAGC1080.14749262536873156No Hit
AAAATAGGGATTTGGTTTGTCTGGAAATTTCCAGATGTAGAAATATCATGTC1070.14612695291161368No Hit
TTCTTAGGGACCAAACTGATTGTGCAATTACTCCTTTTGTAGAAGAGGCCAA1070.14612695291161368No Hit
TTTCCCGGAGGGGTGCCAAGTCCAAGGCCTCCCACTGGGAGTCATCGCTGAG1070.14612695291161368No Hit
AGTGTAAGGGGACAGAGCACTTGTCTTTGTGGATAACCATGACAATCAACGA1070.14612695291161368No Hit
TATGCTGGGGGAAGAGAAAAGGAGTTGGCTGCAGACAAGGGGAAGGGTTCCA1070.14612695291161368No Hit
TTGTAGGGGAAACATGTACAGGTTGGTTCTATAGATAAATTGTGTGTCACAG1060.1447612804544958No Hit
TTACATGGGGCTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGC1050.1433956079973779No Hit
ATAATAGGGAAAGGAAGAAACTTAACATCATTCTCAATAAATATTTATGAAG1050.1433956079973779No Hit
TCAAAGGGGGCCTGGCTTGGGACTAAATGCCCTGCTCTGAACACGAAGCTTA1050.1433956079973779No Hit
GTCAAAGGGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGG1050.1433956079973779No Hit
AATCCAGGGATACTTCCTAGGTACTTTTCTAAAGATTTTACCATACATATGA1040.14202993554026003No Hit
TCATGGGGGTACAACAAAATTCCAGCTTCTCTCCAGAAAGGTATCACCATCT1030.14066426308314214No Hit
ATGCGCGGGAATCATACCAGAACGTCTAGTATAATTACAGTCATGCATTGCT1030.14066426308314214No Hit
TCACACGGAGAGCCGTAATAGAGTTATGCAAAAATATTAATGTTGTTTTCAT1020.13929859062602426No Hit
TTCTTTGGGCTTCAGAAATCACTCCTTCTAGAAAGAGCCTTTTAAAGCTTTA1020.13929859062602426No Hit
TTATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG1000.1365672457117885No Hit
TTTCTAGGGCGTGTCCCTTAACTTGATTCTAGTAAAAGCAGCAAGCAGTGGT1000.1365672457117885No Hit
TGGTATGGGAACTCAAGAAATAAACTCATCTGTCTCAGAGCTGGTTTGATGT1000.1365672457117885No Hit
GCAGGTAGGGGTCCCTGGCTCGGCTCCCTGCCTCCGCGTCGCAGCCCCCGCC1000.1365672457117885No Hit
TGACTAGGGGGTACGAGGTAACCAATTCTTGTCATGGTGAAATCTGATTGAG1000.1365672457117885No Hit
ATAGTGGGGAGCCCCAGGAGGCAGCTGTTTGGCGAGTCCAGTCCCCCAAGCC1000.1365672457117885No Hit
AATGCAGGGGAATGCCAAAGGCAAGACATCATATGGTGTCTTCTGAAGATTC990.1352015732546706No Hit
CTAGCAGGGATCTAATAGTTATACCCAACTAGATAATAGTAGCTTCTAGACA990.1352015732546706No Hit
GACCATCGGGGGAAGTGTGCGCTGCTGCGCAGGCGCGGTGGTCGGACGACAG990.1352015732546706No Hit
TCGATAGGGGGAAAAATATCCAAATTAATTACTAAAATCGAGAAGAGAAGGC990.1352015732546706No Hit
ATTTTTGGGTGAATTATGGCAGAAAGGAAAAATAAAACATACTTCACATTAG990.1352015732546706No Hit
GCTTCAGGGCCTTCCGGCGGGTGACATTCAGCCGGCGGTTCGGGGCGACGGA970.13247022834043481No Hit
TGGAGGGCTATAGTTGTAGCAGGAATCTTCTTACTCATCCGCTTCCACCCCC970.13247022834043481No Hit
CTCATGGGGAATTATTTACTTTAAAAATTGTACTTCAGAACATCAGATTTTA950.12973888342619908No Hit
TTTCATGGGGTCCCACACAAGAGCCTAGACAGAATAGTGGTATTGATCCCAA950.12973888342619908No Hit
CTCAATGGGATCTCAATCGGGGAACATTCAGAATATAGCATTGGTTGCCTAT950.12973888342619908No Hit
TCCTTTGGGCGATCTAATTGTGGGATATGAAAGATGCTATATTCTATTTCAG940.12837321096908116No Hit
GTTTGGGATTAAACAGAAGAAATCATTTTTTTGCTATGTAATACCTCGCAAC940.12837321096908116No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTAT940.12837321096908116No Hit
ATCTGAAGACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCAC930.12700753851196328No Hit
CACACAGGGAACCTGTGAGGGATGCCTTTAAGAGGGTGGAATCTCACCAGGA930.12700753851196328No Hit
GTGTCGGGGCGATCTTTTGGCGGGCGAGTACACCGACTACGGCGGACTAATC920.1256418660548454No Hit
CGGGATGGGATTTCAGATTTCTGTCATAAAAGCATTTTAAAAGTTCATAAAA920.1256418660548454No Hit
AAACGAGGGCCTGACTTATTCGAGTGGTTTTGTGTGAAGACACTGAAAGTGT920.1256418660548454No Hit
AAACAAGGGGGAGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCAC920.1256418660548454No Hit
CATTTGTGGGACTACACGACACGTACTACGTTGTAGCCCACTTCCACTATGT910.12427619359772753No Hit
GCTTGGGGAGTAGTACTGCTTTACACCGTCACTAGTGCAGAAGACATTCTGG910.12427619359772753No Hit
ATACCAGGGGGGCCTAAAGTGAAGACTGTGCTGTCGTTAAGAGCTTTCGAGG910.12427619359772753No Hit
CAGTAGGAGGGATAGAACGATCATTTGGGGGCACGGAGGATTGGTCAGGAGC900.12291052114060963No Hit
TCGCTGTGGGTGGATGTTTTTCCTATTTCAATTTCTTTTGTGGCATCAAATA890.12154484868349175No Hit
ATTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATC890.12154484868349175No Hit
GCTTAGGGGATTCACACTTAGGCAAGCATACACAGGCACATGGCTTTAAGAA880.12017917622637386No Hit
TTAGTCGGGCTGTCTTCAAATTCTGTTTCATGTTCCTGGCTGCATTCTGTTA880.12017917622637386No Hit
CTAGAAAGGGTAGAAAATAGACTATTTTAATTCTATGCCCTTTTCTATTTTC880.12017917622637386No Hit
GATTATGGGTGGTGCACAGCTTGTGAGGAGGGCAAGGTTGTGACGTTCGAGC880.12017917622637386No Hit
ATAGTTGGGAGAAACACATTGGCTCTGTCAGCATTTGTAATTGTACATTTAT880.12017917622637386No Hit
GTTTACGGGCACTTACTTGGAAATTTTTGGACATTATATTAAATGAGTGCTA870.118813503769256No Hit
TACTGTCGGGGAGTGGCCTTAGTGAGTTTGTTGAAGTGCACATGTTTTTCAA870.118813503769256No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGAACTCGTATGCCGTCT860.1174478313121381No Hit
GTTTATGGGAGAGCAGACTCTGACATCTGACACTTGAGTAAAGGGATGTTTT860.1174478313121381No Hit
GTGCCTGGGATTACAGTGATAAATACATAAATGTGGGGCTCATAAAACCCCT850.11608215885502021No Hit
AAAGCTGGGTAAGTGGAAGCACATAATATTTGTCCTTTTGTGTGTGGCTTAT840.11471648639790233No Hit
GACAATGGGATATACTATTAGCAGAAAAAATTGGAAAAATCACCAATTTTTA830.11335081394078445No Hit
ATAGATGGGCTTCTGAATAAAGGAATTTATTGTCTCAATTTCCTGTCTTACT830.11335081394078445No Hit
GCGATGGGGCACTTTGAGCAGCAAAGATGTAGACAACAAGGGCCAAGAACTT830.11335081394078445No Hit
GGATACGGGGCTAGTGGTGAATGGGAAGCCTCAGGGATCCAGCAGCCAGGCT830.11335081394078445No Hit
ACCGAGGGAGAGAAGACCACACCAAGGAGGCGGCCGCTGGCCCAGGAGAGAA830.11335081394078445No Hit
TGTTAAGGGGCCTGACAGAGCAGGTGGCCATGACGCTGGCCTCGGCCATCAG830.11335081394078445No Hit
ACTATGGGGAGAGGATGATGTAATTAAAAAATGGTCAGTGTGCAAAGAAAAG830.11335081394078445No Hit
TGTGCCGGGCACGCTCTTGCCCATGGCTTCTTTTGAGTTGTGTGTAGCTCAT830.11335081394078445No Hit
CTCTAGCGGGGATTTTTTAAAATATGTGTATAAGTCTGTCATGTGCTAAACA820.11198514148366655No Hit
CATCAGGGGAGAACTTTGAAATTCACTCAGCTTTTCCTTTCATGCTGTTTGT820.11198514148366655No Hit
ATTCGAGGGATAAGTGCATGGGAAATGGGCCCAACCACCGAACAGCTCTTAC810.11061946902654868No Hit
TCAATGGGGGACAGGTAATTATATTTGGAAAACATTTAATGCACTAACTTTA810.11061946902654868No Hit
CATAATGGGGAGTCTAAGGCTCCTTCCTGATGATGCCCCCTTCAGCCTGCTC800.1092537965694308No Hit
CACTATGGGAAGCAGGGTGAGATTTAAACAGGCACTATGGTGAGAAACTGCA800.1092537965694308No Hit
GACTTTGGGATATCTTGGTTGCTTACAAGGTTTGGAAATTATGAGTAAAGCT800.1092537965694308No Hit
ACTATAGGGCAGACAAACAGAAAATCTCCCTCCTCCACAGCTGCACGTGTGT800.1092537965694308No Hit
GCAGCGGGGGACTTTTGGATGAAGTGTGTTGGGGAAGAGGATAAGGTTATAT800.1092537965694308No Hit
CAAGGTGGGAGTAGTGAAAAGTAAAAATAAAAACATCATATCTAGAGTCCAT790.10788812411231291No Hit
CAGCCTGGGTAACAGAGCATGACTCCGTCAAAAAAACCCAATTTTTTTAAAG790.10788812411231291No Hit
TCTTTGGGGAGAAGGTTATACCATCATTGGAGTGGAACAAACTGCCAAAAGT780.10652245165519501No Hit
TTTGATGGGAAAGTAATAAAACTTTTCTTGAGACAAACAAAAAAAAAGTTTG780.10652245165519501No Hit
GCCAGTGGGTGGATATATTTTCCCAAAGAAGGGCATTTCTCCACCTTTTTGT780.10652245165519501No Hit
ATCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCG770.10515677919807713No Hit
GATCGAGGGATATACTCAGTTTTATAAGGTGATACTTTTTAAATAAGCGCTT770.10515677919807713No Hit
CGGGCCGGGAAAATGAATAACTTTACTAAGGTAAAAGTGGGCACTGTGACCT770.10515677919807713No Hit
AGCTCCGGGACCTCACCTTGATCAGAGTATTGTTACAATCACATTTGACGTA770.10515677919807713No Hit
CATCGGGGGACCACTGAAGAAATATGATAATATAAAACCATCAAATTTTATA760.10379110674095926No Hit
GTAACGGGGATCTGTTAAAAGCAAGGAATAACTAGTTTATATTTTCCAATAA760.10379110674095926No Hit
TCATAGGGGGGCCGGGCGGCGGCAGGCGGAGGCCACGCGGACACTGCAGCCG750.10242543428384138No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTC750.10242543428384138No Hit
GTACATGGGTTCCCACAAGGTCTACACATCCAACCTTGGCTCCATCATTTAT750.10242543428384138No Hit
ACACACGGGAGGTTTTGTTGTAACATTGTGATTAGGATTTAAACTAATTCAG740.10105976182672348No Hit
GTATTTGGGGTCATTTATTATGCATAGAAGATAATTGAAAAAATATACAATA740.10105976182672348No Hit
TTAAATGGGAATAAGAGAAATTACTTGTCAGAATATGAGTTGTCCTCAACAA740.10105976182672348No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)