FastQCFastQC Report
Sat 31 Dec 2022
SRR5846502.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846502.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72197
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGC40465.604110974140199No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGCCGTCT31064.302117816529773RNA PCR Primer, Index 30 (95% over 22bp)
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCG10931.5139133204980817No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGA7561.0471349225037052No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTCT5340.7396429214510298No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTCTTATAC5090.7050154438550078No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGAT3160.437691316813718No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGAACTCGTATGC2800.3878277490754463No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATG2760.3822873526600828No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGT2500.34627477596022No Hit
ACTAGGGACACTCTGGTATTCTCCATTATTTTTTATTTCATTAAGAAAAATT2120.29364101001426657No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGCC2070.2867155144950621No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGT1990.2756347216643351No Hit
GATGATGGGTAAAGAATCTATGTTGATCAGAAATGTTCCCTTAGATTGTAAA1890.2617837306259263No Hit
TCTTAGGGGCTGGCACCTGTGTCTACTTGCTCCCGAGAAGAGGAGCGCTCAT1670.23131155034142692No Hit
CTATTTGGGAAATCTCAAAACAGAGCTCAGCTGCAAAAAAGCATATTTTCTG1650.22854135213374516No Hit
GTATATGGGGTCTCTCAGTGCCAAGTTGTCAAAAACATCTCTATTTTGCTTA1590.2202307575106999No Hit
GCCGGTAGGAATAGACGTAGACACACGAGCATATTTCACCTCCGCTACCATA1510.20914996467997285No Hit
GGGTTTGGGGAAGGACTTCTAGGGGCTCCGAGAGTTCCCTGAAATTAGAAAC1460.20222446916076847No Hit
TGGCTCACTGCAACCTCCGCCGCCCGGGTTCGGGCGATTCTCCTGCCTCGGC1440.1994542709530867No Hit
TTAATAGGGGTAGAGCAATGGTTCTTAAACTTTGGTATGCACGCAAATCACC1430.1980691718492458No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGACCTCGTATGC1420.19668407274540495No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCTGTCTC1420.19668407274540495No Hit
TACACGAGGGCAGCTCTTAGTTCACAGCAGCCTGAAAAGGTGATGGCAAAGC1410.19529897364156404No Hit
CGTTATGGGAGTTCATCTGATTTTTACTTTGTTCCCATTTATAATTCAAATA1410.19529897364156404No Hit
GTCAAAGGGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGG1400.19391387453772316No Hit
CAAGCAGGGATGAGAATGTCATTTGCTAATGAATTTTAAAAGCATATATAAA1400.19391387453772316No Hit
CAGCTGGGGGGCCCGCAGCCGGTACTGCGGGACCCACTGCGGATATGGCTGT1390.19252877543388228No Hit
CAACAAGGGAGATGTAATGATTTCATTTTTATAACTATTCAAAATTTTAAGT1360.18837347812235966No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTC1360.18837347812235966No Hit
CAAAGCGGTGTGCCTGGAATTTCTGTGAATGCCCTGTACATGTTGGTTCCTT1360.18837347812235966No Hit
TTCTTTGGGCTTCAGAAATCACTCCTTCTAGAAAGAGCCTTTTAAAGCTTTA1330.18421818081083702No Hit
ACACGGGAAATGCCATGGCAACATCAGGAAGTTACCCATATGGTCTACAAAG1290.1786777843954735No Hit
ACATTAGGGAGAAATTCAAATTTTCAGCATTATTGCTAATGTATGGAAACAA1290.1786777843954735No Hit
CACTCAGGGAGAATTCTCACTGTGATATATAAACTCAGACCCAAACATTAAT1290.1786777843954735No Hit
TTTACCGGGCCACGAAGCTGCTGAATCACAATCTGCATTTTAATAGAATCCC1280.1772926852916326No Hit
CATTAAGGGGGATAAAAGGAAAATAACCGTCTGCCGATGGTCCGTACTTCTT1280.1772926852916326No Hit
AGCTTAGGGGTGGAAACAACTGACCTCAAGAAAGGCTGGAGGCTTCATGGCT1270.17590758618779173No Hit
TTTACTGGGGGGATTAGAACAAAGACCATGCAATCACAGATTTCTTAAAAAT1270.17590758618779173No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTT1250.17313738798011No Hit
ACTAACGGGAACTTTCCTAATAAGGATGTTAGGTTTTCTTCTCTTTCATTAG1250.17313738798011No Hit
AATTAAGGGCGATCTTTGGCTGGGGAAGTCATGAAGTTGATGCTAATAAGCC1250.17313738798011No Hit
TGGTATGGGAACTCAAGAAATAAACTCATCTGTCTCAGAGCTGGTTTGATGT1240.1717522888762691No Hit
TTGTAGGGGAAACATGTACAGGTTGGTTCTATAGATAAATTGTGTGTCACAG1210.16759699156474644No Hit
AGCCTGGGTGACTGAGCGGGACTCCGTCTCAAAAAAAATAAAGAAGTAAAAA1200.16621189246090556No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTC1200.16621189246090556No Hit
TGCTACGGGTATTATTAGTCTTAGATAACTTGAAAAAGACAATTATCACATC1200.16621189246090556No Hit
GTTTCTGGGAAGATTTTGTCTTCATTTAAAACTCTGAATCTATTCCATTGAT1200.16621189246090556No Hit
TCTTATGGGCCACTTCACACCCATTAGGATGGCCGGACTAAAAAAGATGCAT1190.16482679335706468No Hit
ATCGAGCGGGGTCTAGCTGAGGGCATTCCTATTTGTTTCCATTTTGCCTATG1190.16482679335706468No Hit
AAAATAGGAAAATGTTACATCTAGAATAGTGTTAGAATTGACCAAAACTAGA1180.1634416942532238No Hit
TTTAATGGGCCCTACATTACATTTAATGCCTGTCTTAGCCCTGAAAGGTTGG1180.1634416942532238No Hit
CTTCTCGGGTGCTGAGAATGAGAGCGAACTGTACTAAAGGCCATTAAAGCAC1180.1634416942532238No Hit
ATAATAGGGAAAGGAAGAAACTTAACATCATTCTCAATAAATATTTATGAAG1170.16205659514938292No Hit
TATATAGGGATCTCCTCAAGGGCTTGTTAACAAATCAGACTTTACACTATAT1160.16067149604554207No Hit
AGCGCTGGGAACTACGCAAAGGCCCCAACGTTGTAGGCCCCTACGGGCTACT1160.16067149604554207No Hit
TTAGTCGGGCTGTCTTCAAATTCTGTTTCATGTTCCTGGCTGCATTCTGTTA1150.15928639694170119No Hit
TATGCTGGGGGAAGAGAAAAGGAGTTGGCTGCAGACAAGGGGAAGGGTTCCA1150.15928639694170119No Hit
TCACACGGAGAGCCGTAATAGAGTTATGCAAAAATATTAATGTTGTTTTCAT1140.1579012978378603No Hit
TGGAGGGCTATAGTTGTAGCAGGAATCTTCTTACTCATCCGCTTCCACCCCC1140.1579012978378603No Hit
TTACATGGGGCTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGC1120.15513109963017854No Hit
AAATAGGGACAAGGAAACATCATATACATCTCATGCAGACCTCACTGCCCTT1120.15513109963017854No Hit
GCAGGTAGGGGTCCCTGGCTCGGCTCCCTGCCTCCGCGTCGCAGCCCCCGCC1110.15374600052633766No Hit
ATAGTGGGGAGCCCCAGGAGGCAGCTGTTTGGCGAGTCCAGTCCCCCAAGCC1110.15374600052633766No Hit
ATGCGCGGGAATCATACCAGAACGTCTAGTATAATTACAGTCATGCATTGCT1100.15236090142249678No Hit
TCTGCCGGGGAATGTAAACATTAATGTTTCCTTATGGGAAAACAAATAAAGT1090.1509758023186559No Hit
TTTCCCGGAGGGGTGCCAAGTCCAAGGCCTCCCACTGGGAGTCATCGCTGAG1080.14959070321481502No Hit
TAATCGGGGGTCAGAAGTTGGGGTCTCCGTGGGCATTGTGATCCGTCCCAGG1070.14820560411097414No Hit
AATGCAGGGGAATGCCAAAGGCAAGACATCATATGGTGTCTTCTGAAGATTC1070.14820560411097414No Hit
GACCATCGGGGGAAGTGTGCGCTGCTGCGCAGGCGCGGTGGTCGGACGACAG1070.14820560411097414No Hit
GGATACGGGGCTAGTGGTGAATGGGAAGCCTCAGGGATCCAGCAGCCAGGCT1070.14820560411097414No Hit
TGATTTAAAATTCCCAACTGCTCCACAACATAACCTGAGACTGTTTCCTTAT1070.14820560411097414No Hit
TGACTAGGGGGTACGAGGTAACCAATTCTTGTCATGGTGAAATCTGATTGAG1070.14820560411097414No Hit
ATAAGTGGGGAAATCTATCAAACATTCAAAGAAAAACTACTATAAATACTTC1060.14682050500713328No Hit
AATCCAGGGATACTTCCTAGGTACTTTTCTAAAGATTTTACCATACATATGA1040.1440503067994515No Hit
CTAGCTGGGAAGTAAAGTTCTGCATGCTTGATTGAGAAAAAGACTTTTATTC1040.1440503067994515No Hit
GCTTGGGGAGTAGTACTGCTTTACACCGTCACTAGTGCAGAAGACATTCTGG1040.1440503067994515No Hit
AAAATAGGGATTTGGTTTGTCTGGAAATTTCCAGATGTAGAAATATCATGTC1030.1426652076956106No Hit
ATAACTGGGAGGCAAACAAGGAAAGGAAAGGAAAGAAGAAAAGGTGCCTTAG1030.1426652076956106No Hit
AAACAAGGGGGAGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCAC1030.1426652076956106No Hit
CTCAATGGGATCTCAATCGGGGAACATTCAGAATATAGCATTGGTTGCCTAT1030.1426652076956106No Hit
CTAGCAGGGATCTAATAGTTATACCCAACTAGATAATAGTAGCTTCTAGACA1020.14128010859176973No Hit
ATTTTTGGGTGAATTATGGCAGAAAGGAAAAATAAAACATACTTCACATTAG1020.14128010859176973No Hit
CATTTGTGGGACTACACGACACGTACTACGTTGTAGCCCACTTCCACTATGT1020.14128010859176973No Hit
TTTCTAGGGCGTGTCCCTTAACTTGATTCTAGTAAAAGCAGCAAGCAGTGGT1020.14128010859176973No Hit
GCACGGGCCTATTCTACACCCTAGTAGGCTCCCTTCCCCTACTCATCGCACT1010.13989500948792885No Hit
TTTCATGGGGTCCCACACAAGAGCCTAGACAGAATAGTGGTATTGATCCCAA1010.13989500948792885No Hit
ATACCAGGGGGGCCTAAAGTGAAGACTGTGCTGTCGTTAAGAGCTTTCGAGG1010.13989500948792885No Hit
TCGCTGTGGGTGGATGTTTTTCCTATTTCAATTTCTTTTGTGGCATCAAATA1000.13850991038408797No Hit
AGTGTAAGGGGACAGAGCACTTGTCTTTGTGGATAACCATGACAATCAACGA1000.13850991038408797No Hit
TCATGGGGGTACAACAAAATTCCAGCTTCTCTCCAGAAAGGTATCACCATCT1000.13850991038408797No Hit
ATCTGAAGACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCAC1000.13850991038408797No Hit
GCCAAGGGGCTTGTTAACAAATCAGACTTTACACTATATACAGATGTGACAG990.1371248112802471No Hit
TTCTTAGGGACCAAACTGATTGTGCAATTACTCCTTTTGTAGAAGAGGCCAA970.13435461307256533No Hit
TTATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG970.13435461307256533No Hit
TCGATAGGGGGAAAAATATCCAAATTAATTACTAAAATCGAGAAGAGAAGGC950.1315844148648836No Hit
GTTTACGGGCACTTACTTGGAAATTTTTGGACATTATATTAAATGAGTGCTA950.1315844148648836No Hit
CTCATGGGGAATTATTTACTTTAAAAATTGTACTTCAGAACATCAGATTTTA940.1301993157610427No Hit
CACACAGGGAACCTGTGAGGGATGCCTTTAAGAGGGTGGAATCTCACCAGGA930.12881421665720183No Hit
ATTAGAGGGTGAGTTGAGCAGGATTATTCATAGGAATAAAGTAAGAAAAAAA920.12742911755336095No Hit
ATGCTAGGGGGGACTTGTGAGGACTCACATAAATGCTGTTGTGGCAAAGATT920.12742911755336095No Hit
CAAGCAGGGGTGTGGTGGAGTTGGACAGAATGAGATGATAGTGAAAACTAGC920.12742911755336095No Hit
AGCTAGGGATTCATGCATGTTATGTGTATCAATAGTTCATTCCTTTTTATTG910.12604401844952007No Hit
GATTATGGGTGGTGCACAGCTTGTGAGGAGGGCAAGGTTGTGACGTTCGAGC900.12465891934567919No Hit
ATAGATGGGCTTCTGAATAAAGGAATTTATTGTCTCAATTTCCTGTCTTACT890.1232738202418383No Hit
CGGGCCGGGAAAATGAATAACTTTACTAAGGTAAAAGTGGGCACTGTGACCT890.1232738202418383No Hit
GTTTATGGGAGAGCAGACTCTGACATCTGACACTTGAGTAAAGGGATGTTTT890.1232738202418383No Hit
GACAATGGGATATACTATTAGCAGAAAAAATTGGAAAAATCACCAATTTTTA880.12188872113799741No Hit
TCATAGGGGGGCCGGGCGGCGGCAGGCGGAGGCCACGCGGACACTGCAGCCG880.12188872113799741No Hit
AAACGAGGGCCTGACTTATTCGAGTGGTTTTGTGTGAAGACACTGAAAGTGT880.12188872113799741No Hit
GCTTCAGGGCCTTCCGGCGGGTGACATTCAGCCGGCGGTTCGGGGCGACGGA870.12050362203415653No Hit
CACTATGGGAAGCAGGGTGAGATTTAAACAGGCACTATGGTGAGAAACTGCA870.12050362203415653No Hit
TCAATGGGGGACAGGTAATTATATTTGGAAAACATTTAATGCACTAACTTTA870.12050362203415653No Hit
GTTTGGGATTAAACAGAAGAAATCATTTTTTTGCTATGTAATACCTCGCAAC870.12050362203415653No Hit
ATAGTTGGGAGAAACACATTGGCTCTGTCAGCATTTGTAATTGTACATTTAT870.12050362203415653No Hit
ATTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATC860.11911852293031568No Hit
TGTGCCGGGCACGCTCTTGCCCATGGCTTCTTTTGAGTTGTGTGTAGCTCAT860.11911852293031568No Hit
CTAGAAAGGGTAGAAAATAGACTATTTTAATTCTATGCCCTTTTCTATTTTC850.1177334238264748No Hit
GCTTAGGGGATTCACACTTAGGCAAGCATACACAGGCACATGGCTTTAAGAA840.11634832472263391No Hit
AAAGCTGGGTAAGTGGAAGCACATAATATTTGTCCTTTTGTGTGTGGCTTAT840.11634832472263391No Hit
ACTATGGGGAGAGGATGATGTAATTAAAAAATGGTCAGTGTGCAAAGAAAAG840.11634832472263391No Hit
GCCAGTGGGTGGATATATTTTCCCAAAGAAGGGCATTTCTCCACCTTTTTGT840.11634832472263391No Hit
GTGTCGGGGCGATCTTTTGGCGGGCGAGTACACCGACTACGGCGGACTAATC830.11496322561879303No Hit
GAAGGAGGGCGTTCATGTTGTTTTCAGTTCATGGTATGATTAAAACCCGATC830.11496322561879303No Hit
ACTATAGGGCAGACAAACAGAAAATCTCCCTCCTCCACAGCTGCACGTGTGT830.11496322561879303No Hit
ATGTTTGGGGTCCAAGAATAGAGAGATAGCATGGCCCCTGTCAACGCTTCCT830.11496322561879303No Hit
CGGGATGGGATTTCAGATTTCTGTCATAAAAGCATTTTAAAAGTTCATAAAA820.11357812651495215No Hit
GCAGCGGGGGACTTTTGGATGAAGTGTGTTGGGGAAGAGGATAAGGTTATAT820.11357812651495215No Hit
GCGATGGGGCACTTTGAGCAGCAAAGATGTAGACAACAAGGGCCAAGAACTT810.11219302741111127No Hit
TTTCCTGGGGACTGTCTTCCCAAGCCCCATGTTCATGGTGGGGCAATGGTTA800.11080792830727038No Hit
ATTCGAGGGATAAGTGCATGGGAAATGGGCCCAACCACCGAACAGCTCTTAC800.11080792830727038No Hit
TCCTTTGGGCGATCTAATTGTGGGATATGAAAGATGCTATATTCTATTTCAG800.11080792830727038No Hit
GTATTTGGGGTCATTTATTATGCATAGAAGATAATTGAAAAAATATACAATA800.11080792830727038No Hit
GCGTATGGGGGGGGCGCAAACCCTTTTCCTCCCAGCCTATTTTTGTAAATAA800.11080792830727038No Hit
CATAATGGGGAGTCTAAGGCTCCTTCCTGATGATGCCCCCTTCAGCCTGCTC790.1094228292034295No Hit
GATCGAGGGATATACTCAGTTTTATAAGGTGATACTTTTTAAATAAGCGCTT790.1094228292034295No Hit
GTACAAGGGTATGGTAATGCTTGTAGAATCTTTATTATCTCAACAATCTAAA780.10803773009958861No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTC780.10803773009958861No Hit
CGTACAGTGGGGGTGTGCTTTTAATGAGCTGTGTTCATATGTGTTGGGGAAA770.10665263099574773No Hit
GCTGTGCGGGTCCTATTTTATTAGCACAAACTTGCTAGCTAATTAGAGTTTA760.10526753189190688No Hit
TGTTAAGGGGCCTGACAGAGCAGGTGGCCATGACGCTGGCCTCGGCCATCAG760.10526753189190688No Hit
GACTTTGGGATATCTTGGTTGCTTACAAGGTTTGGAAATTATGAGTAAAGCT750.103882432788066No Hit
CATCAGGGGAGAACTTTGAAATTCACTCAGCTTTTCCTTTCATGCTGTTTGT750.103882432788066No Hit
CATCGGGGGACCACTGAAGAAATATGATAATATAAAACCATCAAATTTTATA740.10249733368422512No Hit
TCTCGCGGGAGCTGAAACTGATTAGAAAATTCTTTATATTTTAAAATAGCTC740.10249733368422512No Hit
TCAAAGGGGGCCTGGCTTGGGACTAAATGCCCTGCTCTGAACACGAAGCTTA740.10249733368422512No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGACCTCGTATGCCGTCT740.10249733368422512No Hit
TCTTTGGGGAGAAGGTTATACCATCATTGGAGTGGAACAAACTGCCAAAAGT740.10249733368422512No Hit
GACAGTGGGTTTGCATTTCTAAAATGGTGACTTTTATTCTGCAAGAGTTCTT730.10111223458038424No Hit
TCAATGGGGGTGATAAACTGGTGTTTTTTGGATGCTTCAAGCACACGTTAAC730.10111223458038424No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)