FastQCFastQC Report
Sat 31 Dec 2022
SRR5846501.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846501.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71639
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGC41095.73570262008124No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGCCGTCT29804.159745390080822RNA PCR Primer, Index 30 (95% over 22bp)
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCG11261.5717695668560423No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGA7281.0162062563687377No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTCT5030.7021315205404878No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTCTTATAC4660.6504836750931755No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGAT3550.4955401387512388No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGAACTCGTATGC3390.4732059353145633No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATG2960.4131827635784978No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGCC2460.34338837783888665No Hit
ACTAGGGACACTCTGGTATTCTCCATTATTTTTTATTTCATTAAGAAAAATT2290.31965828668741886No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGT2210.30849118496908107No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGT2090.29174053239157444No Hit
GATGATGGGTAAAGAATCTATGTTGATCAGAAATGTTCCCTTAGATTGTAAA1870.26103100266614554No Hit
ACACGGGAAATGCCATGGCAACATCAGGAAGTTACCCATATGGTCTACAAAG1690.23590502379988557No Hit
CTATTTGGGAAATCTCAAAACAGAGCTCAGCTGCAAAAAAGCATATTTTCTG1600.2233420343667555No Hit
CGTTATGGGAGTTCATCTGATTTTTACTTTGTTCCCATTTATAATTCAAATA1520.21217493264841775No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTT1470.20519549407445664No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCTGTCTC1470.20519549407445664No Hit
GGGTTTGGGGAAGGACTTCTAGGGGCTCCGAGAGTTCCCTGAAATTAGAAAC1450.20240371864487222No Hit
GTATATGGGGTCTCTCAGTGCCAAGTTGTCAAAAACATCTCTATTTTGCTTA1440.20100783093008No Hit
TCTTAGGGGCTGGCACCTGTGTCTACTTGCTCCCGAGAAGAGGAGCGCTCAT1430.19961194321528775No Hit
TAATCGGGGGTCAGAAGTTGGGGTCTCCGTGGGCATTGTGATCCGTCCCAGG1420.19821605550049554No Hit
CTTCTCGGGTGCTGAGAATGAGAGCGAACTGTACTAAAGGCCATTAAAGCAC1370.19123661692653443No Hit
TTAATAGGGGTAGAGCAATGGTTCTTAAACTTTGGTATGCACGCAAATCACC1370.19123661692653443No Hit
TTGTAGGGGAAACATGTACAGGTTGGTTCTATAGATAAATTGTGTGTCACAG1340.18704895378215775No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTC1320.1842571783525733No Hit
GCCGGTAGGAATAGACGTAGACACACGAGCATATTTCACCTCCGCTACCATA1310.1828612906377811No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGACCTCGTATGC1300.18146540292298888No Hit
CAACAAGGGAGATGTAATGATTTCATTTTTATAACTATTCAAAATTTTAAGT1290.18006951520819664No Hit
CTAGCTGGGAAGTAAAGTTCTGCATGCTTGATTGAGAAAAAGACTTTTATTC1280.17867362749340443No Hit
TGCTACGGGTATTATTAGTCTTAGATAACTTGAAAAAGACAATTATCACATC1280.17867362749340443No Hit
ACTAACGGGAACTTTCCTAATAAGGATGTTAGGTTTTCTTCTCTTTCATTAG1280.17867362749340443No Hit
GTCAAAGGGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGG1270.17727773977861222No Hit
CAAGCAGGGATGAGAATGTCATTTGCTAATGAATTTTAAAAGCATATATAAA1260.17588185206382No Hit
AGCTTAGGGGTGGAAACAACTGACCTCAAGAAAGGCTGGAGGCTTCATGGCT1260.17588185206382No Hit
TATATAGGGATCTCCTCAAGGGCTTGTTAACAAATCAGACTTTACACTATAT1250.17448596434902777No Hit
TGATTTAAAATTCCCAACTGCTCCACAACATAACCTGAGACTGTTTCCTTAT1250.17448596434902777No Hit
CAAAGCGGTGTGCCTGGAATTTCTGTGAATGCCCTGTACATGTTGGTTCCTT1250.17448596434902777No Hit
AATTAAGGGCGATCTTTGGCTGGGGAAGTCATGAAGTTGATGCTAATAAGCC1250.17448596434902777No Hit
TTTACCGGGCCACGAAGCTGCTGAATCACAATCTGCATTTTAATAGAATCCC1240.17309007663423556No Hit
TGGCTCACTGCAACCTCCGCCGCCCGGGTTCGGGCGATTCTCCTGCCTCGGC1240.17309007663423556No Hit
GTTTCTGGGAAGATTTTGTCTTCATTTAAAACTCTGAATCTATTCCATTGAT1240.17309007663423556No Hit
CATTAAGGGGGATAAAAGGAAAATAACCGTCTGCCGATGGTCCGTACTTCTT1230.17169418891944332No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTC1200.16750652577506667No Hit
TTCTTTGGGCTTCAGAAATCACTCCTTCTAGAAAGAGCCTTTTAAAGCTTTA1200.16750652577506667No Hit
TACACGAGGGCAGCTCTTAGTTCACAGCAGCCTGAAAAGGTGATGGCAAAGC1190.16611063806027443No Hit
ATTCGAGGGATAAGTGCATGGGAAATGGGCCCAACCACCGAACAGCTCTTAC1190.16611063806027443No Hit
AGCGCTGGGAACTACGCAAAGGCCCCAACGTTGTAGGCCCCTACGGGCTACT1190.16611063806027443No Hit
TCTGCCGGGGAATGTAAACATTAATGTTTCCTTATGGGAAAACAAATAAAGT1180.16471475034548222No Hit
AGCTAGGGATTCATGCATGTTATGTGTATCAATAGTTCATTCCTTTTTATTG1170.16331886263068998No Hit
TGGTATGGGAACTCAAGAAATAAACTCATCTGTCTCAGAGCTGGTTTGATGT1160.16192297491589777No Hit
TGTGCCGGGCACGCTCTTGCCCATGGCTTCTTTTGAGTTGTGTGTAGCTCAT1160.16192297491589777No Hit
AGCCTGGGTGACTGAGCGGGACTCCGTCTCAAAAAAAATAAAGAAGTAAAAA1150.16052708720110553No Hit
CACTCAGGGAGAATTCTCACTGTGATATATAAACTCAGACCCAAACATTAAT1150.16052708720110553No Hit
AAAATAGGGATTTGGTTTGTCTGGAAATTTCCAGATGTAGAAATATCATGTC1140.15913119948631332No Hit
TTCTTAGGGACCAAACTGATTGTGCAATTACTCCTTTTGTAGAAGAGGCCAA1140.15913119948631332No Hit
TCACACGGAGAGCCGTAATAGAGTTATGCAAAAATATTAATGTTGTTTTCAT1140.15913119948631332No Hit
TGGAGGGCTATAGTTGTAGCAGGAATCTTCTTACTCATCCGCTTCCACCCCC1140.15913119948631332No Hit
ATAATAGGGAAAGGAAGAAACTTAACATCATTCTCAATAAATATTTATGAAG1130.1577353117715211No Hit
CTCATGGGGAATTATTTACTTTAAAAATTGTACTTCAGAACATCAGATTTTA1130.1577353117715211No Hit
GCCAAGGGGCTTGTTAACAAATCAGACTTTACACTATATACAGATGTGACAG1130.1577353117715211No Hit
TTACATGGGGCTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGC1120.15633942405672888No Hit
TTTAATGGGCCCTACATTACATTTAATGCCTGTCTTAGCCCTGAAAGGTTGG1100.15354764862714443No Hit
ATCGAGCGGGGTCTAGCTGAGGGCATTCCTATTTGTTTCCATTTTGCCTATG1090.1521517609123522No Hit
TCATGGGGGTACAACAAAATTCCAGCTTCTCTCCAGAAAGGTATCACCATCT1080.15075587319755998No Hit
CAAGCAGGGGTGTGGTGGAGTTGGACAGAATGAGATGATAGTGAAAACTAGC1070.14935998548276777No Hit
GGATACGGGGCTAGTGGTGAATGGGAAGCCTCAGGGATCCAGCAGCCAGGCT1060.14796409776797553No Hit
ATAAGTGGGGAAATCTATCAAACATTCAAAGAAAAACTACTATAAATACTTC1060.14796409776797553No Hit
CTAGCAGGGATCTAATAGTTATACCCAACTAGATAATAGTAGCTTCTAGACA1050.14656821005318332No Hit
GACCATCGGGGGAAGTGTGCGCTGCTGCGCAGGCGCGGTGGTCGGACGACAG1050.14656821005318332No Hit
TTTACTGGGGGGATTAGAACAAAGACCATGCAATCACAGATTTCTTAAAAAT1050.14656821005318332No Hit
ATAGTGGGGAGCCCCAGGAGGCAGCTGTTTGGCGAGTCCAGTCCCCCAAGCC1050.14656821005318332No Hit
AAAATAGGAAAATGTTACATCTAGAATAGTGTTAGAATTGACCAAAACTAGA1040.1451723223383911No Hit
TCTTATGGGCCACTTCACACCCATTAGGATGGCCGGACTAAAAAAGATGCAT1040.1451723223383911No Hit
AAACAAGGGGGAGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCAC1040.1451723223383911No Hit
TTATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG1030.14377643462359888No Hit
TATGCTGGGGGAAGAGAAAAGGAGTTGGCTGCAGACAAGGGGAAGGGTTCCA1020.14238054690880667No Hit
AAATAGGGACAAGGAAACATCATATACATCTCATGCAGACCTCACTGCCCTT1010.14098465919401446No Hit
AAAGCTGGGTAAGTGGAAGCACATAATATTTGTCCTTTTGTGTGTGGCTTAT990.13819288376443No Hit
CTAGAAAGGGTAGAAAATAGACTATTTTAATTCTATGCCCTTTTCTATTTTC990.13819288376443No Hit
GTTTACGGGCACTTACTTGGAAATTTTTGGACATTATATTAAATGAGTGCTA990.13819288376443No Hit
GATTATGGGTGGTGCACAGCTTGTGAGGAGGGCAAGGTTGTGACGTTCGAGC990.13819288376443No Hit
ATGCTAGGGGGGACTTGTGAGGACTCACATAAATGCTGTTGTGGCAAAGATT980.13679699604963777No Hit
ATTTTTGGGTGAATTATGGCAGAAAGGAAAAATAAAACATACTTCACATTAG970.13540110833484556No Hit
ACATTAGGGAGAAATTCAAATTTTCAGCATTATTGCTAATGTATGGAAACAA970.13540110833484556No Hit
AGTGTAAGGGGACAGAGCACTTGTCTTTGTGGATAACCATGACAATCAACGA970.13540110833484556No Hit
CAGCTGGGGGGCCCGCAGCCGGTACTGCGGGACCCACTGCGGATATGGCTGT970.13540110833484556No Hit
CATAATGGGGAGTCTAAGGCTCCTTCCTGATGATGCCCCCTTCAGCCTGCTC960.13400522062005332No Hit
TTTCCCGGAGGGGTGCCAAGTCCAAGGCCTCCCACTGGGAGTCATCGCTGAG960.13400522062005332No Hit
TTTCTAGGGCGTGTCCCTTAACTTGATTCTAGTAAAAGCAGCAAGCAGTGGT960.13400522062005332No Hit
GATGGTGGGGGGGCATGATGGGGATTGCTGCGTATCAGCAGTTGGATGTTCT940.13121344519046887No Hit
ACTATGGGGAGAGGATGATGTAATTAAAAAATGGTCAGTGTGCAAAGAAAAG940.13121344519046887No Hit
CTCAATGGGATCTCAATCGGGGAACATTCAGAATATAGCATTGGTTGCCTAT940.13121344519046887No Hit
ATCTGAAGACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCAC940.13121344519046887No Hit
ATGCGCGGGAATCATACCAGAACGTCTAGTATAATTACAGTCATGCATTGCT940.13121344519046887No Hit
GCTTAGGGGATTCACACTTAGGCAAGCATACACAGGCACATGGCTTTAAGAA930.12981755747567666No Hit
AATCCAGGGATACTTCCTAGGTACTTTTCTAAAGATTTTACCATACATATGA930.12981755747567666No Hit
GCTTCAGGGCCTTCCGGCGGGTGACATTCAGCCGGCGGTTCGGGGCGACGGA920.12842166976088443No Hit
CATTTGTGGGACTACACGACACGTACTACGTTGTAGCCCACTTCCACTATGT920.12842166976088443No Hit
ATTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATC920.12842166976088443No Hit
GAAGGAGGGCGTTCATGTTGTTTTCAGTTCATGGTATGATTAAAACCCGATC910.12702578204609222No Hit
TACTGTCGGGGAGTGGCCTTAGTGAGTTTGTTGAAGTGCACATGTTTTTCAA910.12702578204609222No Hit
AATGCAGGGGAATGCCAAAGGCAAGACATCATATGGTGTCTTCTGAAGATTC900.12562989433129998No Hit
GCAGTCTTTCCCTTTTTGAGGTCTGTAGAATACTTGGGTGGAAAAAAACTTG900.12562989433129998No Hit
ATAACTGGGAGGCAAACAAGGAAAGGAAAGGAAAGAAGAAAAGGTGCCTTAG900.12562989433129998No Hit
GCACGGGCCTATTCTACACCCTAGTAGGCTCCCTTCCCCTACTCATCGCACT900.12562989433129998No Hit
TCGCTGTGGGTGGATGTTTTTCCTATTTCAATTTCTTTTGTGGCATCAAATA890.12423400661650777No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTAT890.12423400661650777No Hit
GCTTGGGGAGTAGTACTGCTTTACACCGTCACTAGTGCAGAAGACATTCTGG880.12283811890171555No Hit
GTTTGGGATTAAACAGAAGAAATCATTTTTTTGCTATGTAATACCTCGCAAC880.12283811890171555No Hit
ATCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCG870.12144223118692332No Hit
CGGGCCGGGAAAATGAATAACTTTACTAAGGTAAAAGTGGGCACTGTGACCT870.12144223118692332No Hit
ACCGAGGGAGAGAAGACCACACCAAGGAGGCGGCCGCTGGCCCAGGAGAGAA870.12144223118692332No Hit
ATAGTTGGGAGAAACACATTGGCTCTGTCAGCATTTGTAATTGTACATTTAT870.12144223118692332No Hit
CACTATGGGAAGCAGGGTGAGATTTAAACAGGCACTATGGTGAGAAACTGCA860.1200463434721311No Hit
TCAATGGGGGACAGGTAATTATATTTGGAAAACATTTAATGCACTAACTTTA860.1200463434721311No Hit
TCGATAGGGGGAAAAATATCCAAATTAATTACTAAAATCGAGAAGAGAAGGC850.11865045575733889No Hit
TTTCATGGGGTCCCACACAAGAGCCTAGACAGAATAGTGGTATTGATCCCAA850.11865045575733889No Hit
TTAGTCGGGCTGTCTTCAAATTCTGTTTCATGTTCCTGGCTGCATTCTGTTA850.11865045575733889No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGAACTCGTATGCCGTCT850.11865045575733889No Hit
GCAGGTAGGGGTCCCTGGCTCGGCTCCCTGCCTCCGCGTCGCAGCCCCCGCC840.11725456804254665No Hit
TTTGATGGGAAAGTAATAAAACTTTTCTTGAGACAAACAAAAAAAAAGTTTG830.11585868032775444No Hit
TCGACTGGGGTAATACATGAATATTGTCTCTTTCAAATGAAGCAAATCTTCA820.1144627926129622No Hit
GACAATGGGATATACTATTAGCAGAAAAAATTGGAAAAATCACCAATTTTTA820.1144627926129622No Hit
TCCTTTGGGCGATCTAATTGTGGGATATGAAAGATGCTATATTCTATTTCAG820.1144627926129622No Hit
GTAACGGGGATCTGTTAAAAGCAAGGAATAACTAGTTTATATTTTCCAATAA820.1144627926129622No Hit
GTTTATGGGAGAGCAGACTCTGACATCTGACACTTGAGTAAAGGGATGTTTT820.1144627926129622No Hit
ATAGATGGGCTTCTGAATAAAGGAATTTATTGTCTCAATTTCCTGTCTTACT810.11306690489816999No Hit
GCGATGGGGCACTTTGAGCAGCAAAGATGTAGACAACAAGGGCCAAGAACTT810.11306690489816999No Hit
CGGGATGGGATTTCAGATTTCTGTCATAAAAGCATTTTAAAAGTTCATAAAA810.11306690489816999No Hit
TGACTAGGGGGTACGAGGTAACCAATTCTTGTCATGGTGAAATCTGATTGAG810.11306690489816999No Hit
AAAATGGGGCTTCCGATCTGACATGGGGGATCTCTGCATACGGGAGGAGGGA800.11167101718337775No Hit
AGTCCAGTGTGAGCTTGAAATACTCCTAGATCCCGTCAGTGATTTTTATATA800.11167101718337775No Hit
TTTCATTGGGGTGGCGCAGTCAGAGATAAATGGAACAGAGTCTACAAATAAA800.11167101718337775No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTC800.11167101718337775No Hit
GTACAAGGGTATGGTAATGCTTGTAGAATCTTTATTATCTCAACAATCTAAA790.11027512946858554No Hit
GAGACAGGGAGAAGGAGCTTTTTGCTTATCTATAATTTTTCACTGCCAATTT790.11027512946858554No Hit
TCAAAGGGGGCCTGGCTTGGGACTAAATGCCCTGCTCTGAACACGAAGCTTA790.11027512946858554No Hit
ATTAGAGGGTGAGTTGAGCAGGATTATTCATAGGAATAAAGTAAGAAAAAAA780.10887924175379332No Hit
TCTCGCGGGAGCTGAAACTGATTAGAAAATTCTTTATATTTTAAAATAGCTC780.10887924175379332No Hit
TTAAATGGGAATAAGAGAAATTACTTGTCAGAATATGAGTTGTCCTCAACAA780.10887924175379332No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGACCTCGTATGCCGTCT770.1074833540390011No Hit
CATCAGGGGAGAACTTTGAAATTCACTCAGCTTTTCCTTTCATGCTGTTTGT770.1074833540390011No Hit
CAGCCTGGGTAACAGAGCATGACTCCGTCAAAAAAACCCAATTTTTTTAAAG760.10608746632420887No Hit
AATAGGGGATCTAGCCCCAAGCCCATATCTGTACACTTTTGTTCATTTTATA760.10608746632420887No Hit
ATACCAGGGGGGCCTAAAGTGAAGACTGTGCTGTCGTTAAGAGCTTTCGAGG760.10608746632420887No Hit
TTTCCTGGGGACTGTCTTCCCAAGCCCCATGTTCATGGTGGGGCAATGGTTA750.10469157860941666No Hit
GTGTCGGGGCGATCTTTTGGCGGGCGAGTACACCGACTACGGCGGACTAATC750.10469157860941666No Hit
CAGTAGGAGGGATAGAACGATCATTTGGGGGCACGGAGGATTGGTCAGGAGC750.10469157860941666No Hit
GCCAGTGGGTGGATATATTTTCCCAAAGAAGGGCATTTCTCCACCTTTTTGT750.10469157860941666No Hit
TTCATAGGGGAGTTTTACTACTGAAAAAATTGGATTAATAAAGTAAAATATA740.10329569089462445No Hit
CAAGGTGGGAGTAGTGAAAAGTAAAAATAAAAACATCATATCTAGAGTCCAT730.10189980317983222No Hit
CGGCGGGGGAGCTTCATGTTGAAGTGGTTTTTTGTTTTTGTTTTTGTTTTTT730.10189980317983222No Hit
GCGTATGGGGGGGGCGCAAACCCTTTTCCTCCCAGCCTATTTTTGTAAATAA730.10189980317983222No Hit
TGTTAAGGGGCCTGACAGAGCAGGTGGCCATGACGCTGGCCTCGGCCATCAG730.10189980317983222No Hit
ACTATAGGGCAGACAAACAGAAAATCTCCCTCCTCCACAGCTGCACGTGTGT720.10050391546504No Hit
AAACGAGGGCCTGACTTATTCGAGTGGTTTTGTGTGAAGACACTGAAAGTGT720.10050391546504No Hit
GTACATGGGTTCCCACAAGGTCTACACATCCAACCTTGGCTCCATCATTTAT720.10050391546504No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)