FastQCFastQC Report
Sat 31 Dec 2022
SRR5846497.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846497.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79393
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGC38164.806469084176187No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGCCGTCT33044.1615759575781235RNA PCR Primer, Index 30 (95% over 22bp)
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCG10481.3200156185054097No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGT8691.0945549355736652No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGA6710.8451626717720706No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTCTTATAC5870.7393598931895758No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATG5230.658748252364818No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTCT5170.651190911037497No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGT3940.49626541382741557No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGCC3390.4269897849936392No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGAT2880.36275238371141033No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGAACTCGTATGC2720.34259947350522085No Hit
CGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCG2290.28843852732608666No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTC1940.24435403625004726No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGACCTCGTATGC1710.21538422782864988No Hit
CGGTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCT1620.2040482158376683No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTC1430.18011663496781832No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGT1280.1612232816495157No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTC1280.1612232816495157No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCTGTCTC1270.15996372476162887No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTAT1220.15366594032219466No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGACCTCGTATGCCGTCT1080.13603214389177887No Hit
GTACCGGGGCGCTATACTGCACTTACGCTATCCAGTTGCAGGTAATTTTTTG1000.12595568878868413No Hit
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTAT980.12343657501291046No Hit
CCACAGGGACCTGTTTTCTTCTAGCCTTTTCCCATTGCTGTGATCCTAGTTC980.12343657501291046No Hit
ATTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATC900.11336011990981573No Hit
CTACCTGGGATATCTTCTGCCCCCACACATGCATAGTCTCCTTCATTATCAA830.10454322169460784No Hit
ATCTAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGA830.10454322169460784No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGAACTCGTATGCCGTCT830.10454322169460784No Hit
ATTTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGAT810.10202410791883415No Hit
GAATGCGGGGGTGTCATCATTCACCAGTTTGTGAAGGGAGTTAAAGGAAAGT810.10202410791883415No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)