FastQCFastQC Report
Sat 31 Dec 2022
SRR5846496.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846496.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78643
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGC39174.980735729817021No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGCCGTCT33264.229238457332503RNA PCR Primer, Index 30 (95% over 22bp)
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCG10081.281741540887301No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGT8961.1393258141220453No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGA6280.7985453250766119No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTCTTATAC6050.7692992383301755No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATG5960.7578551174293962No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTCT4960.6306982185318465No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGT3500.44504914614142393No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGCC2930.37256971376982057No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGAT2760.3509530409572372No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGAACTCGTATGC2560.3255216611777272No Hit
CGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCG2190.2784736085856338No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTC1750.22252457307071197No Hit
CGGTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCT1740.22125300408173645No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTC1430.18183436542349604No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTC1410.17929122744554507No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCTGTCTC1390.17674808946759407No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGACCTCGTATGC1220.15513141665501062No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGT1210.1538598476660351No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTAT1180.15004514069910863No Hit
ATTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATC1000.1271568988975497No Hit
GAATGCGGGGGTGTCATCATTCACCAGTTTGTGAAGGGAGTTAAAGGAAAGT960.1220706229416477No Hit
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTAT950.12079905395267221No Hit
CCACAGGGACCTGTTTTCTTCTAGCCTTTTCCCATTGCTGTGATCCTAGTTC940.1195274849636967No Hit
CTACCTGGGATATCTTCTGCCCCCACACATGCATAGTCTCCTTCATTATCAA910.11571277799677021No Hit
CGTTTAGGGCTGATGATGAAGAATTACGGCATCGAATCCGGCAAAAACAGGA890.11316964001881923No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGACCTCGTATGCCGTCT860.10935493305189271No Hit
ATCTAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGA800.10172551911803976No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)