FastQCFastQC Report
Sat 31 Dec 2022
SRR5846495.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846495.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78477
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGC36094.598799648304599No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGCCGTCT31544.0190119398040185RNA PCR Primer, Index 30 (95% over 22bp)
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCG10891.3876677243013877No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGT8731.1124278451011125No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATG6370.8117027919008116No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGA6370.8117027919008116No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTCTTATAC5870.7479898569007479No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTCT4460.5683193802005683No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGT3540.45108757980045106No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGCC3100.395020197000395No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGAACTCGTATGC3090.3937459383003937No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGAT2940.3746320578003747No Hit
CGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCG2080.265045809600265No Hit
CGGTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCT1580.20133287460020133No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTC1460.18604177020018603No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTC1420.18094473540018094No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTC1300.16565363100016567No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCTGTCTC1300.16565363100016567No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGT1290.16437937230016436No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTAT1290.16437937230016436No Hit
CCACAGGGACCTGTTTTCTTCTAGCCTTTTCCCATTGCTGTGATCCTAGTTC1210.15418530270015418No Hit
ATTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATC1170.1490882679001491No Hit
GAATGCGGGGGTGTCATCATTCACCAGTTTGTGAAGGGAGTTAAAGGAAAGT1110.14144271570014144No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGACCTCGTATGC1080.13761993960013763No Hit
CTACCTGGGATATCTTCTGCCCCCACACATGCATAGTCTCCTTCATTATCAA960.12232883520012232No Hit
ATCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCG890.11340902430011342No Hit
GTCAATGGGATGAGGAGGCCACCTAAATTGATCAGAATATAAGCTTATCCTA860.10958624820010959No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGACCTCGTATGCCGTCT820.10448921340010447No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGAACTCGTATGCCGTCT800.10194069600010194No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)