FastQCFastQC Report
Sat 31 Dec 2022
SRR5846493.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846493.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103694
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGC50714.890350454221074No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGCCGTCT42364.085096534032828RNA PCR Primer, Index 30 (95% over 22bp)
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCG12701.2247574594479913No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGA9440.9103708989912628No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTCTTATAC6160.5940555866298918No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTCT5740.5535517966323992No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGAT3960.38189287711921616No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGAACTCGTATGC3850.37128474164368236No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGT3130.3018496730765522No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATG2800.2700252666499508No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGCC2380.2295214766524582No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTT2130.20541207784442686No Hit
CTAATAGGGCAGTTTTCTCTTTTTTATATTTTCTGGGGGTTTTTTGTTGAGA1910.18419580689335932No Hit
AGATCTGGGCTAACAAAATCTTTGTAAGTTCTGTATGCTGAAACCTACAGAA1730.16683703975157674No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGACCTCGTATGC1710.16490828784693426No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCTGTCTC1660.16008640808532798No Hit
AATTTAGGGGATTTTTGTGTAGACATATGTTTTTATTTTTCTTGGGTAACTA1620.15622890427604297No Hit
ACTAAGGGGACTTCTGGTGGTTACCCTGACTCTTGACTCTTAGAAGTGCCCG1560.15044264856211545No Hit
ATGGTTGGGCTATGAGAGATACAAAAATGAATTAGACACAGGTTTTGTTGTC1540.14851389665747294No Hit
GTGGATGGGGAGACGAGGCTGCGCCCGGATTCCGGTCCGCAGGGAGACCGAA1530.1475495207051517No Hit
GTTTTGGGGATCAACAAATATGGCTGCTAGTATCTCTACTAGTATAAGAAAA1460.14079888903890292No Hit
GCTCAGGGAGTATCGTCTGCATTCCAGCAAAAATATCAACTCGTAAGGCACT1430.13790576118193917No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTC1420.1369413852296179No Hit
TAGGGTGGGCTGAGTACTTTGAAAACATAATAGTTATGAAATTGATATTATA1420.1369413852296179No Hit
GCCTTGGGGGCAGTGAGACTGACTGCGTCGGGGTTGAGACTGGGTGGATGAG1420.1369413852296179No Hit
TCATGGGGGACCAGCCAATGCTGGTGCAATGGGAAATATAGTAGGTATAAAT1420.1369413852296179No Hit
GTGACAGGGGGTCCAGCTCAGCTGCCTGGCTGCGACAGCAGGGTGCCACCGA1420.1369413852296179No Hit
AACATGGGGAGCTGATGTCAATGCTATGGACTGCACAAAAGTCTCAATGTTC1410.13597700927729667No Hit
ATAGCTGGGGGCCCATGGGGGGATAAAAATGTCAAATTATATGTCTTCCAGT1410.13597700927729667No Hit
TAAAACGGGGATCCATTTCAAAGCAGAAGCTGTGGAATGGTATTAAATATTT1380.13308388142033292No Hit
CCACAGGTAGGCTTAGGTTTATGTGTGTGTATGTGTCTTAGTTTTTAACAAA1380.13308388142033292No Hit
TTAATGGGGAGGGAAATTAAGTAACTTGCCATGGTCACCTGCATAGTGATAG1370.13211950546801163No Hit
ATATGAGGGCAAAAGGAAGTGACATCACGAGTGGCCAGACTGAAATTTAGTG1370.13211950546801163No Hit
TTCTAGGGGAAGAAAAATGTGAATTTGGTGGTTTTATTCTATCGGTATAAAG1360.1311551295156904No Hit
AACGCTGGGGGGTTGTTTATTTGAAGTTTTTCTTTTGTGATGTAGGCACTAA1360.1311551295156904No Hit
ACTCAGGGGCTATCAGGGATGGTTGAATGTAGCATTTATGGTTGAATGTAGA1360.1311551295156904No Hit
GAGTAGGGGGGGCATTCACCTAGTCTTAATTGAATGCTAGGTACTCTACATG1340.12922637761104788No Hit
CTTCTTGGGCAAAGAAGGGGCAAGCTCTTTTGCCTTTTAGGCCAGACATAGC1330.12826200165872664No Hit
TGTATTGGGATTCATTCATTCTTTTAGCAAACCAAAGACAGTGTATATATGT1330.12826200165872664No Hit
ACAAGTGGGGGGATGTTTTTGTGTGTGATGACACCTCAGTAATGCTGCAGAA1320.1272976257064054No Hit
ATAAGTGGGGATGTCCCTGCATGGAGCTGGTGAATTACTAAAAGAACTTGGC1290.12440449784944163No Hit
GGCATTATCAGGTACCTCCAATAGTGGGGAGATGGTTTTCTTTCTGAGGGAA1280.12344012189712038No Hit
TCAACTGGGAAGTTATGGGTGGAGAAGCCTGGGTATGACTTTCCGTTGTGTA1280.12344012189712038No Hit
ATTAGTGGGATAGATGGAATTAGACATGGCATTAAGTGACTTGGAGGCTGCA1280.12344012189712038No Hit
GGTTTTGGGCTTTTCCGATCTATTAAAGGGTCCACATGCGTGTGGCAGCCCA1270.12247574594479912No Hit
TAACTGGGGCTTATTTGATGTTTAAGAGCAACTTGTGTGATCTTAGCTTTGC1270.12247574594479912No Hit
GCCAGTGGGCAGATCAAAAATTCATTTAATTGTATTACTGTTTTGTTCCTCA1270.12247574594479912No Hit
TAACAATGGGAAGTAAAATTATATTTTCCATCATCTTTTATGCTAAAGGAAT1260.12151136999247786No Hit
ACAATAGGGCAAAGCATAATGTGTAGCATATCAGCAATAACTACATATTTAT1260.12151136999247786No Hit
ATGAGGGGACTGGAAATCTACATAAATGTCAGTAAACCAAATAAAAGTTCAT1250.1205469940401566No Hit
GTATAAGGGAGCATGAAGAGGAAAAAAGACAAAAACTAGAATTCATTGGAAA1250.1205469940401566No Hit
GAGCACGGGATAGAAGTAAAAAAACAATAATGCATATTTCCTCATATCATAA1240.11958261808783537No Hit
GGTTGGGAAGGAGAGCCAAAGCTAAGACCCCCGAAACCAGACGAGCTACCTA1240.11958261808783537No Hit
GATAGTGGGATGGATATAATTTGCAGCCCATGGATATAACTGGTTGATAAGC1240.11958261808783537No Hit
AAGAATGGGTATGTAGAATGGATACTCATACAATGTTTGAAATTTGAAATTA1240.11958261808783537No Hit
ATCCTGGGGATTTTCACATTTGACATTTCATTCACATTCATTTCACATTTCA1230.11861824213551411No Hit
TCAAAGGGAATTATAAACACAAAATTGTTAGTGTTTATGATGTTTTTATCAT1230.11861824213551411No Hit
TCAGCTAGGGGAGTGCTTATTGAGTAAAGCATAATTCGGTCATAATGAAATC1230.11861824213551411No Hit
AGAACTGGGGGGCTCCGAAGCGGGGAAGTGGGACAAGATGGTTTACATCTCG1220.11765386618319286No Hit
TTTTATGGGGTTTAAATTATGAACAGAATATGAGTGATGCCATGGCAATTTT1220.11765386618319286No Hit
ACTACAGGGCAGAGTTGAAAATGCTTTTAGGTGGCATCATAGAACCATCCAT1220.11765386618319286No Hit
ATCGAAGGGAGTGCATTCCTTTGATGAGAAAACAAGGAAGATTTCCTTTCGT1220.11765386618319286No Hit
TCCTAGGGGTACTCTTCCAAAATACATCATTTGCTGCCTGACTTAGACCTAG1210.11668949023087159No Hit
GAGATGGGGTATGCCTGTGAAATTTGAGAATGGAACTTGGGTGGTTCTTACA1210.11668949023087159No Hit
CATGTAGGGCATCCAGTTTGGTTGCTGTGGAGCAACATGCATATTGGATTAG1210.11668949023087159No Hit
TTTAGAGGGCATGGAGTTTATTTTATATCATATTTCCCTTTTTTATTTCTGC1200.11572511427855034No Hit
ATCATACGGGTTATGACTTAAACCAACTACAACTTCCCTATAGCTTCTAAGC1200.11572511427855034No Hit
TTAATTGGGACCACTGAAGAACCTGCTCATGTAACCAAATACCACCTGTTCC1190.1147607383262291No Hit
ATCGCTGGGAACAAACAAACAACAATAACAAAAAAACTGGTTTACTGAAAAA1190.1147607383262291No Hit
CTTTCACCGGGATAGTGATACTTAAACAGGTGAGGAGGCAATGCTTTTCTAT1180.11379636237390785No Hit
TGTGGGGGGCTGACAGAATTCCAGACCAACCTGGTGCCCTACCCCCGCATCC1170.1128319864215866No Hit
TAATGCGGGAAGACTCCATCTCAACAACAACAACAAAAAGGAATTAAGGAGG1160.11186761046926533No Hit
ACTGCTGGGAGCTGGGATTACAGGCGCCCACCACAACACCTGGCTAATTTTT1160.11186761046926533No Hit
TTCTAGGGGTGAGGAAGGAAACTATGAGGAGGAAGGAAGTGAAGATGTCTGG1160.11186761046926533No Hit
TGACTAGGGGGAAGACTTCATTGCTATCTTTAAAGATATCCTAGTGCCAGTG1150.11090323451694407No Hit
TATTTCGGGGAAGGGTGAGCTGTACATCATGACTTCTTTCTAAGGGTGCAGA1140.10993885856462284No Hit
TGGCATGGGATAGCTGCTTTTATGTGTATTTTATATTGCATGCTTTTGTAAA1130.10897448261230158No Hit
GTGCAAGGGGGAGTTCCCATTAGACTTGAAGTTGGGCCACGTGATATGAAGA1120.10801010665998033No Hit
TGTTTGGGGGTAAGGTGAAGGAATATTGAGGCTGGCCCCCTACATTTAGGAT1120.10801010665998033No Hit
ATTACAGGGCGATCTGTAGGCGGGCCAAGGAGGCATCGCAATACTTCATCCT1120.10801010665998033No Hit
ACAGGGGATCCAGCAAGCAGTCTACCCTGTCCTTTGCAATTGCTCTTCTCCA1120.10801010665998033No Hit
GTAAGTGGGACAAGGCAAAAACTATTGAACAGTTGGTTTTAGTGTGTTGTAT1120.10801010665998033No Hit
ACAGCAGGGAGAGATACAGATATTTCTTGGAATGGTTTGAATAGTAAATAAA1120.10801010665998033No Hit
GATCTAGGGTGTTCACTGTTTCTAGAAAAAAATCATTGCCATAAGAAAAAGT1120.10801010665998033No Hit
GCGACAGGGGTTATTGGTGATGCTCTTGCATTTAGTCATAATTTGGAAGATG1110.10704573070765906No Hit
TCTAGTGGGTGGCTTCTCTTAGGAAAAGACGACTTCCTAGTCATAGGTGTCC1110.10704573070765906No Hit
GCAGCAGGGATAGCAGAGAGAACTATGTAAATTATCTTGCAGAACAAAATAG1110.10704573070765906No Hit
CGGAGCGGGGTGACCTACAAAGAGACTTAGACTCCCACACATTAATAATGGG1110.10704573070765906No Hit
TTGCCGGGGATTGATGAATTTTCATAAGGTGGAATTGATCTTAATCTACTAA1100.10608135475533781No Hit
AATCTCGGGCATGGGTTTATTGCTAGAAAGATCCAGGGGATAGCTTTAGGTT1100.10608135475533781No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTC1090.10511697880301657No Hit
TTCATAGGGCACATGTAGTCATGAAGGTGATATCAGAGGTAGCCTTTTCTGG1090.10511697880301657No Hit
TGTTTGGGAAATCATGCATGCAGATTAAATCTTTTACAATAAAAAATTCTCT1090.10511697880301657No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGACCTCGTATGCCGTCT1090.10511697880301657No Hit
GTAATGGGGAGAGGATGATGTAATTAAAAAATGGTCAGTGTGCAAAGAAAAG1090.10511697880301657No Hit
TCCGTAGGGATACATATATATGCACCCAGCATTGGAACACCCTGATACATAA1090.10511697880301657No Hit
AATTCAGGGGGGTGGAGCTAAGTCCATTGATAGTTCCAGTATAGAAAGTGCA1090.10511697880301657No Hit
CACGGGGCACAAAAATTAGCTGGGTGTGATGGCGTGTGCCTGTTAATTCCAG1090.10511697880301657No Hit
TTAGCTGGGAGTACAAATATTAACAATTATATTAACACCTGCCTCATGTCAG1090.10511697880301657No Hit
TATATTGGGTACAGATTTCTGGCAGAACCCTGAATTTCACAATCAGTCAGTA1080.10415260285069532No Hit
GCAATGGGGATTCTTGCCAGTGATAGACAACAATTGAAAACCATGATGTTTC1080.10415260285069532No Hit
CGCCTTGGGAGGGGGAAAGGGTCACCTTAAAAATAAATTATTTTCAGGGACT1080.10415260285069532No Hit
AGAACCGGGTCTCCTTTTGGAGCTCTGGCTTTACCCCAGCTTCCATGCTTGT1080.10415260285069532No Hit
ACCCATGGGAAAGATGCCATTAAAAAGAAATTTACAGGTATTAAACATGAGT1080.10415260285069532No Hit
TAGATAGGGACGCTTGAGCTCGGTAGGTGGAGGTTGCAGTGAGCCAGGATCG1080.10415260285069532No Hit
ATCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCG1070.10318822689837406No Hit
ATAGCGGGGCAGAACAGCATGTGGGCTCTGGGCCTTGGCAGGTGCACAGACG1070.10318822689837406No Hit
ACTTAAGGGTATATTTGACTAAACCTTTTCCTAAGCTATCTCTCATAGTTCC1070.10318822689837406No Hit
GGCATTGGGCCAAATCCAAAAGCTTCATTTTTATTTTTGGTATTTAATAAGA1070.10318822689837406No Hit
ATTCAGGGGATTCCTGGAAAGGCACATGCCGAAGAGGAATATGGTATAATTT1070.10318822689837406No Hit
AGGTATGGGGAAGTTCTGCTGTTCGAAAGAGCTACATTCTTGGTTATTTCCC1070.10318822689837406No Hit
TACGTAGGGCTTCCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATGA1070.10318822689837406No Hit
ACACGTGGGAGCACTCTTTGTTGAATAGACTGTCTTTGCTCCATTGTATTGT1060.1022238509460528No Hit
CTTTATGGGTAAACAATCCATGAATAAATTCCCTTTGTATCATCAGTGGGGC1060.1022238509460528No Hit
AACAATGGGATTCTGTAACCAAGATCATCTTGACACAGTATTTATGTTGTTT1050.10125947499373154No Hit
AGTATTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1050.10125947499373154No Hit
ATATTGGGGAGGCATCCAGGGTGTATCCTGCCACTGTGCAGTGCCCTGCTTT1040.1002950990414103No Hit
GCTGCAGGGATATTTAAGTTGAACTTTATATATTGAATTCTTCGTTCTATAG1040.1002950990414103No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)