FastQCFastQC Report
Sat 31 Dec 2022
SRR5846492.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846492.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102871
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGC48754.738944892146475No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGCCGTCT41664.04973218885789RNA PCR Primer, Index 30 (95% over 22bp)
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCG12961.2598302728660167No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGA9470.9205704231513255No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTCTTATAC6000.5832547559564892No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTCT5720.5560362006785197No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGAT4340.42188760680852716No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGAACTCGTATGC4140.40244578160997757No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATG3550.3450923972742561No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGT3280.3188459332562141No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGCC2300.22358098978332083No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGACCTCGTATGC1850.17983688308658416No Hit
AGATCTGGGCTAACAAAATCTTTGTAAGTTCTGTATGCTGAAACCTACAGAA1850.17983688308658416No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTT1810.17594851804687425No Hit
CCACAGGTAGGCTTAGGTTTATGTGTGTGTATGTGTCTTAGTTTTTAACAAA1790.17400433552701927No Hit
CTAATAGGGCAGTTTTCTCTTTTTTATATTTTCTGGGGGTTTTTTGTTGAGA1720.1671996967075269No Hit
GAGTAGGGGGGGCATTCACCTAGTCTTAATTGAATGCTAGGTACTCTACATG1600.15553460158839713No Hit
GCTCAGGGAGTATCGTCTGCATTCCAGCAAAAATATCAACTCGTAAGGCACT1500.1458136889891223No Hit
ATCATACGGGTTATGACTTAAACCAACTACAACTTCCCTATAGCTTCTAAGC1470.14289741520933985No Hit
GGCATTATCAGGTACCTCCAATAGTGGGGAGATGGTTTTCTTTCTGAGGGAA1450.1409532326894849No Hit
ACAATAGGGCAAAGCATAATGTGTAGCATATCAGCAATAACTACATATTTAT1450.1409532326894849No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTC1440.13998114142955742No Hit
TAAAACGGGGATCCATTTCAAAGCAGAAGCTGTGGAATGGTATTAAATATTT1430.13900905016962992No Hit
TAACAATGGGAAGTAAAATTATATTTTCCATCATCTTTTATGCTAAAGGAAT1410.13706486764977496No Hit
TTGCCGGGGATTGATGAATTTTCATAAGGTGGAATTGATCTTAATCTACTAA1380.13414859386999253No Hit
AATTTAGGGGATTTTTGTGTAGACATATGTTTTTATTTTTCTTGGGTAACTA1380.13414859386999253No Hit
AGAACTGGGGGGCTCCGAAGCGGGGAAGTGGGACAAGATGGTTTACATCTCG1360.13220441135013755No Hit
ATGGTTGGGCTATGAGAGATACAAAAATGAATTAGACACAGGTTTTGTTGTC1350.13123232009021008No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCTGTCTC1350.13123232009021008No Hit
TAATGCGGGAAGACTCCATCTCAACAACAACAACAAAAAGGAATTAAGGAGG1350.13123232009021008No Hit
GCCAGTGGGCAGATCAAAAATTCATTTAATTGTATTACTGTTTTGTTCCTCA1330.1292881375703551No Hit
ACTAAGGGGACTTCTGGTGGTTACCCTGACTCTTGACTCTTAGAAGTGCCCG1310.12734395505050014No Hit
GTTTTGGGGATCAACAAATATGGCTGCTAGTATCTCTACTAGTATAAGAAAA1290.1253997725306452No Hit
ACCCATGGGAAAGATGCCATTAAAAAGAAATTTACAGGTATTAAACATGAGT1290.1253997725306452No Hit
TCATGGGGGACCAGCCAATGCTGGTGCAATGGGAAATATAGTAGGTATAAAT1280.12442768127071771No Hit
TAGATAGGGACGCTTGAGCTCGGTAGGTGGAGGTTGCAGTGAGCCAGGATCG1280.12442768127071771No Hit
GGGCGCGGGAACCACGGCGGTGAAGCAGTGGGAGCAGCGCTGGATCAAGAAC1270.1234555900107902No Hit
AACATGGGGAGCTGATGTCAATGCTATGGACTGCACAAAAGTCTCAATGTTC1270.1234555900107902No Hit
ATCGCTGGGAACAAACAAACAACAATAACAAAAAAACTGGTTTACTGAAAAA1270.1234555900107902No Hit
ATCCTGGGGATTTTCACATTTGACATTTCATTCACATTCATTTCACATTTCA1250.12151140749093525No Hit
ACTCAGGGGCTATCAGGGATGGTTGAATGTAGCATTTATGGTTGAATGTAGA1250.12151140749093525No Hit
TGTGGGGGGCTGACAGAATTCCAGACCAACCTGGTGCCCTACCCCCGCATCC1240.12053931623100776No Hit
TAGGGTGGGCTGAGTACTTTGAAAACATAATAGTTATGAAATTGATATTATA1230.11956722497108029No Hit
ATAGCTGGGGGCCCATGGGGGGATAAAAATGTCAAATTATATGTCTTCCAGT1230.11956722497108029No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTC1220.11859513371115281No Hit
TCAGCTAGGGGAGTGCTTATTGAGTAAAGCATAATTCGGTCATAATGAAATC1220.11859513371115281No Hit
CGGAGCGGGGTGACCTACAAAGAGACTTAGACTCCCACACATTAATAATGGG1220.11859513371115281No Hit
TCTAGTGGGTGGCTTCTCTTAGGAAAAGACGACTTCCTAGTCATAGGTGTCC1210.11762304245122532No Hit
ATATGAGGGCAAAAGGAAGTGACATCACGAGTGGCCAGACTGAAATTTAGTG1210.11762304245122532No Hit
TTAGCTGGGAGTACAAATATTAACAATTATATTAACACCTGCCTCATGTCAG1200.11665095119129784No Hit
CTTCTTGGGCAAAGAAGGGGCAAGCTCTTTTGCCTTTTAGGCCAGACATAGC1180.11470676867144287No Hit
TTCTAGGGGTGAGGAAGGAAACTATGAGGAGGAAGGAAGTGAAGATGTCTGG1180.11470676867144287No Hit
TTTTATGGGGTTTAAATTATGAACAGAATATGAGTGATGCCATGGCAATTTT1170.1137346774115154No Hit
AACGCTGGGGGGTTGTTTATTTGAAGTTTTTCTTTTGTGATGTAGGCACTAA1160.11276258615158792No Hit
GGCATTGGGCCAAATCCAAAAGCTTCATTTTTATTTTTGGTATTTAATAAGA1160.11276258615158792No Hit
TGTAGCGGGTGCAAATTTTAAGAAATACAGGTGCATGACCAATTGTCAATTA1160.11276258615158792No Hit
TTCTAGGGGAAGAAAAATGTGAATTTGGTGGTTTTATTCTATCGGTATAAAG1150.11179049489166042No Hit
CACGCAGTGGGCATCCTTTTCCTCTATGTAGTCATACAAACAGTTTGTATAT1150.11179049489166042No Hit
GTAAGTGGGACAAGGCAAAAACTATTGAACAGTTGGTTTTAGTGTGTTGTAT1150.11179049489166042No Hit
TCAAAGGGAATTATAAACACAAAATTGTTAGTGTTTATGATGTTTTTATCAT1150.11179049489166042No Hit
TATTTCGGGGAAGGGTGAGCTGTACATCATGACTTCTTTCTAAGGGTGCAGA1140.11081840363173295No Hit
TAACTGGGGCTTATTTGATGTTTAAGAGCAACTTGTGTGATCTTAGCTTTGC1140.11081840363173295No Hit
ACAAGTGGGGGGATGTTTTTGTGTGTGATGACACCTCAGTAATGCTGCAGAA1140.11081840363173295No Hit
CTTTCACCGGGATAGTGATACTTAAACAGGTGAGGAGGCAATGCTTTTCTAT1140.11081840363173295No Hit
GAGCACGGGATAGAAGTAAAAAAACAATAATGCATATTTCCTCATATCATAA1130.10984631237180548No Hit
ATAAGTGGGGATGTCCCTGCATGGAGCTGGTGAATTACTAAAAGAACTTGGC1130.10984631237180548No Hit
CATGTAGGGCATCCAGTTTGGTTGCTGTGGAGCAACATGCATATTGGATTAG1120.10887422111187797No Hit
ACACGTGGGAGCACTCTTTGTTGAATAGACTGTCTTTGCTCCATTGTATTGT1120.10887422111187797No Hit
ACAGTTGGGTGATCAGATTTATCATATTCATTGAATATTATTTTATTGCACT1120.10887422111187797No Hit
GAGTATGGGATATAAGGTGGCATATGTTAAGTTTTGCTTCATTGGACCGTTT1110.1079021298519505No Hit
CGCCTTGGGAGGGGGAAAGGGTCACCTTAAAAATAAATTATTTTCAGGGACT1110.1079021298519505No Hit
TCCGTAGGGATACATATATATGCACCCAGCATTGGAACACCCTGATACATAA1110.1079021298519505No Hit
GTGACAGGGGGTCCAGCTCAGCTGCCTGGCTGCGACAGCAGGGTGCCACCGA1110.1079021298519505No Hit
ACAGGGGATCCAGCAAGCAGTCTACCCTGTCCTTTGCAATTGCTCTTCTCCA1100.10693003859202302No Hit
ATCGAAGGGAGTGCATTCCTTTGATGAGAAAACAAGGAAGATTTCCTTTCGT1100.10693003859202302No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGAACTCGTATGCCGTCT1100.10693003859202302No Hit
ATCTGAAGACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCAC1100.10693003859202302No Hit
CACGGGGCACAAAAATTAGCTGGGTGTGATGGCGTGTGCCTGTTAATTCCAG1100.10693003859202302No Hit
GGTTTTGGGCTTTTCCGATCTATTAAAGGGTCCACATGCGTGTGGCAGCCCA1090.10595794733209553No Hit
AACGCAGGGATATTAAATATGCTACTGACTTTGAAATGCCTACAGATTTGCA1090.10595794733209553No Hit
ACAATGGGGAGTGCATAGGCTGAAATGTCTCAAGATTCCCCACACTACATAT1090.10595794733209553No Hit
TTAATTGGGACCACTGAAGAACCTGCTCATGTAACCAAATACCACCTGTTCC1090.10595794733209553No Hit
GCAGCAGGGATAGCAGAGAGAACTATGTAAATTATCTTGCAGAACAAAATAG1090.10595794733209553No Hit
GTATAAGGGAGCATGAAGAGGAAAAAAGACAAAAACTAGAATTCATTGGAAA1090.10595794733209553No Hit
ACTCTAGGGCTGGGAGAAGAATTGCCTCTCAAAGCTGCTTCTATAAGCAGCC1080.10498585607216805No Hit
ACCGTGGGGAGTGGTACTTGGAATCCTGTTTTAATTTGCACAAATTTAAATG1070.10401376481224058No Hit
GCTAAAGGGAATAATTTTATAGTAAAATTTAAACTATTTCTCAAGGAAGTTA1070.10401376481224058No Hit
ATGTCAGGGATGGGGATGAGGATGAAGAACTTTTGTAGAAATCGGGGTTCCC1070.10401376481224058No Hit
GGCACCGGGTAGTAAAACCAATGAATGTTTAACTCCTTCATAAGCAAAATTA1070.10401376481224058No Hit
GCAATGGGGATTCTTGCCAGTGATAGACAACAATTGAAAACCATGATGTTTC1070.10401376481224058No Hit
ACTACAGGGCAGAGTTGAAAATGCTTTTAGGTGGCATCATAGAACCATCCAT1070.10401376481224058No Hit
GCCTTGGGGGCAGTGAGACTGACTGCGTCGGGGTTGAGACTGGGTGGATGAG1070.10401376481224058No Hit
GCCCACGGTAAGAAATTGGACTACATAAAAATGTAAAATATGTTTACATTAG1050.10206958229238561No Hit
ACTTAAGGGTATATTTGACTAAACCTTTTCCTAAGCTATCTCTCATAGTTCC1050.10206958229238561No Hit
GTAATGGGGAGAGGATGATGTAATTAAAAAATGGTCAGTGTGCAAAGAAAAG1050.10206958229238561No Hit
CTTGCTGGGAGTTTCACTAGAATAAGACCATGCCTGTTGACTAAGTTCAAGA1050.10206958229238561No Hit
AACAATGGGATTCTGTAACCAAGATCATCTTGACACAGTATTTATGTTGTTT1040.10109749103245813No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGACCTCGTATGCCGTCT1040.10109749103245813No Hit
GATCTAGGGTGTTCACTGTTTCTAGAAAAAAATCATTGCCATAAGAAAAAGT1040.10109749103245813No Hit
AATTCAGGGGGGTGGAGCTAAGTCCATTGATAGTTCCAGTATAGAAAGTGCA1040.10109749103245813No Hit
GAGATGGGGTATGCCTGTGAAATTTGAGAATGGAACTTGGGTGGTTCTTACA1030.10012539977253064No Hit
TGTTTGGGGGTAAGGTGAAGGAATATTGAGGCTGGCCCCCTACATTTAGGAT1030.10012539977253064No Hit
GCGACTGGGTTAACAATTTTCAATATGTTCATATTTTAATTCACAATGGAAA1030.10012539977253064No Hit
AATTTAGGGATGTTGACTGAATGACTATGAGGAAAAACAAGTATTATTAACA1030.10012539977253064No Hit
TAACAAGGGATGACTGCCTCTCTCACCTAACTGCCAGTATTAGCTTTTCAGG1030.10012539977253064No Hit
AGCGCTGGGAAAATAGAATTGATTAATTCTTCCTATGATCAAATAAATAGGA1030.10012539977253064No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)