FastQCFastQC Report
Sat 31 Dec 2022
SRR5846491.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846491.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences381260
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGC97832.5659654828725804No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGCCGTCT94072.4673451188165556RNA PCR Primer, Index 30 (95% over 22bp)
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCG21730.5699522635471856No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGA16730.43880816240885484No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTCTTATAC10910.28615642868383784No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTCT10630.27881235902009127No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGAACTCGTATGC7460.19566699889838954No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGAT6860.17992970676178985No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATG5690.14924198709542044No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGACCTCGTATGC4880.12799664271101086No Hit
TTGTGAGGGCATGTACTATTTCTTAAAACTAACCTACCTGAAAACTGTCTAA4740.12432460787913759No Hit
ATGCCTGGGAAAAACACAAATAACATTTAATTTTATTCATTCTGCTTCAAAA4620.12117714945181765No Hit
GCACAAGGGCACAGCACTTTCTCAGACTCACTTATAAGAGTGCGTTTTTATC4600.12065257304726433No Hit
CCGGAAGCTAGAAGCAGATGATTTTTTCCAAAGCTGCGTACTTTTCACTGAG4480.1175051146199444No Hit
ACAAATGGGATTACAAAACTGAATGCAAATGTCCTTTGTGGTGCTTGTTCCT4410.11566909720400777No Hit
GGCCGCGGGCTAGTGTTGGTGGTATGTAACAAGAGTTACGTATCCAGGGGCT4400.1154068090017311No Hit
AACCGAGGGATGGGTCAGAAGACTCAATATCTTAAGATCGGTTTTCTTCAAA4360.11435765619262446No Hit
AGTTTTGGGTATCTTGGGGTCTTCCATGTCAGTAGAGAGATATACATCTCTT4320.11330850338351782No Hit
GTACGTGGGGAAATAAAGTTTATTTGTAGTAAATTAGGATAATAGTAATTTC4290.11252163877668783No Hit
TTAACGGGGGGCCGTCTTCTGTCTGCTGTGTGGGAAGCGCTTCCAGGCGCAG4290.11252163877668783No Hit
GTTCTTGGGAACCCTACTGAGTAATTGGCTCCATTAAAACAAGAGAGAATTG4280.11225935057441117No Hit
TGTGCCGGGAGTGGTAGGAGTTCTTTGTATCTTCTGGATACTAAACCCTTAT4270.1119970623721345No Hit
TGTCAGGGGATGGCATGTATAGCTCCATGATTCTCTTCATCAGCTCTGTGAC4240.11121019776530452No Hit
TCCACAGGGGGCATTTGCAGAGTGAGACAAAGCGGAGAACGCTGGTGGGCCT4230.11094790956302784No Hit
TGGCATGGGGGAGGAAATTCAGAAGAAATTAGAAGCTGCAGAAGAAAGACGC4220.11068562136075119No Hit
GGGCACGGTTTGTGACAGGTACTTCTGGTTGTGGCTTTGTCCATGGAGACGA4220.11068562136075119No Hit
GGCTGCGGGATCTATCGCCGGGAGCAGATGATGGATGTCTAGAAAACACCCC4200.11016104495619786No Hit
TCATAGGGGCTTCCTGCTAGTTGGCGACCTCATGGCTGTGAACAACTGCGGG4180.10963646855164455No Hit
ATTCAGGGGAACCTCGAAGAGCAAACAGAAAGAAATGGTGATGTGGCAAACC4160.10911189214709123No Hit
ACCATAGGGATACATTATGTCATTTGATGCACTCTCTCAGAAAGCCATTGAA4100.10753816293343126No Hit
TGCTACGGGCTGATTAAGTTATGTCAAAATGCTGAGCCAGGCATAGCTGAAG4080.10701358652887792No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGT4080.10701358652887792No Hit
GCGCGCGGGTAAAGAAAAAGATTGGTCATTTGACTATATAAAAATTTAAAAC4070.10675129832660127No Hit
TGATATGGGGTAAGTGAGGACAGGCACTTCTGTTTTTGTGCCCAAATATTAC4050.10622672192204795No Hit
TGTGCAGGGCACGACGCTCTTCTCGTCGGGGGATTTTCATGTATACCAAGAC4040.1059644337197713No Hit
AAGCTTGGGTTCATATATACATATATGAGTGCATTTTAATGATGTGCTGATA4000.10491528091066463No Hit
GTTGGCGGGTCCCCGCCGCCGGGACATACCGCGGGGGGGCGCTGCGGCCTGC3980.10439070450611132No Hit
GCTTATGGGCAGTGAAATTCTGCCTGAAGAAACCTCTTATTGAATTTGAAAA3950.10360383989928133No Hit
TTGCACGGGCATTTTTCAGAGTCAGCAGTTTCAAATCTTATTGTTATCATGT3940.10334155169700467No Hit
GTTCACGGGAGTGGCAACTGAGAATGAAGTCTTCCGAGTGAAGGTTTTTAAT3930.10307926349472801No Hit
TGCCCAGGGGTACATATTTAACGCAAACTTCAGCATCCTTTAAATTTTAAAG3920.10281697529245136No Hit
TTGCCTGGGCTAATTATAAGTGAAGTTCAGCATTGTTTCATGTGCTTATTGA3890.10203011068562137No Hit
GGCGCAGGGTCAAGAAGGGAGATGTGGTCATTGTGCTGACCGGATGGCGCCC3870.10150553428106804No Hit
AGCCTGGGGGACAGAGCGAGACTGTCTCAAAAACAAACAAACAAACAAACAA3860.10124324607879139No Hit
AAAAACGGGATTGTAGGAATCGAATAAATCCATATAAAATCCAAGTGATAAA3820.10019409326968473No Hit
GTTAATGGGAAGCCAGTCACAGAAGAAAAATTTTTGCATGATTCTACTTATA3820.10019409326968473No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)