FastQCFastQC Report
Sat 31 Dec 2022
SRR5846490.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846490.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences381415
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGC97642.5599412713186425No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGCCGTCT91812.407089390821022RNA PCR Primer, Index 30 (95% over 22bp)
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCG20920.5484839348216509No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGA17000.4457087424458923No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTCT11910.31225830132532806No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTCTTATAC11510.3017710367971894No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGAACTCGTATGC7290.19113039602532675No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGAT6450.1691071405162356No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATG5510.1444620688751098No Hit
ATGCCTGGGAAAAACACAAATAACATTTAATTTTATTCATTCTGCTTCAAAA5090.1334504411205642No Hit
TTAACGGGGGGCCGTCTTCTGTCTGCTGTGTGGGAAGCGCTTCCAGGCGCAG5070.13292607789415728No Hit
CCGGAAGCTAGAAGCAGATGATTTTTTCCAAAGCTGCGTACTTTTCACTGAG5040.13213953305454687No Hit
ATTCAGGGGAACCTCGAAGAGCAAACAGAAAGAAATGGTGATGTGGCAAACC4880.12794462724329142No Hit
ACAAATGGGATTACAAAACTGAATGCAAATGTCCTTTGTGGTGCTTGTTCCT4730.12401190304523943No Hit
ACGTGGGGATTACAATTCAAGATGAGATCCGTATCAGGTGCCAATAAAAAAT4710.12348753981883251No Hit
AACCGAGGGATGGGTCAGAAGACTCAATATCTTAAGATCGGTTTTCTTCAAA4630.12139008691320477No Hit
TGGCATGGGGGAGGAAATTCAGAAGAAATTAGAAGCTGCAGAAGAAAGACGC4510.11824390755476319No Hit
TGCTACGGGCTGATTAAGTTATGTCAAAATGCTGAGCCAGGCATAGCTGAAG4470.11719518110194932No Hit
TTGTGAGGGCATGTACTATTTCTTAAAACTAACCTACCTGAAAACTGTCTAA4460.11693299948874586No Hit
AGCCTGGGGGACAGAGCGAGACTGTCTCAAAAACAAACAAACAAACAAACAA4410.11562209142272852No Hit
GCACAAGGGCACAGCACTTTCTCAGACTCACTTATAAGAGTGCGTTTTTATC4370.11457336496991466No Hit
TGTCAGGGGATGGCATGTATAGCTCCATGATTCTCTTCATCAGCTCTGTGAC4370.11457336496991466No Hit
TTGTAGGGACTCTGAACTTTTTACAAAGAAAAATGGAAAAACTTTGTATGGT4360.11431118335671119No Hit
TGTGCCGGGAGTGGTAGGAGTTCTTTGTATCTTCTGGATACTAAACCCTTAT4300.11273809367749041No Hit
TAGTTAGGGATCCCCAAGGGCACAACCATAATGGCATTACTGACTTCCGTGC4270.11195154883788No Hit
TAGCTGGGGAGGCTGTCTCTGGATGGATTCTGGTGGATGAATGGCAACGCGG4260.11168936722467653No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGT4240.1111650039982696No Hit
CATGCCGGGCTCAGCACACTGCAACCTCTGCCTCCTGGGTTCAAGCAATTCT4230.11090282238506613No Hit
AGTTCTGGGGAAAAAAGCAATCCTAGTTTCTGACAAAACAGACTTTAAACCA4230.11090282238506613No Hit
TCATAGGGGCTTCCTGCTAGTTGGCGACCTCATGGCTGTGAACAACTGCGGG4180.1095919143190488No Hit
ATGTTTGGGAGCCTTTATCTGGTTACAGAGTTTTTTTTCTGTAAAGAAATTA4160.10906755109264188No Hit
ACCATAGGGATACATTATGTCATTTGATGCACTCTCTCAGAAAGCCATTGAA4150.1088053694794384No Hit
TCCACAGGGGGCATTTGCAGAGTGAGACAAAGCGGAGAACGCTGGTGGGCCT4150.1088053694794384No Hit
GCTAATGGGGATTAATATGGATTTTTAAATCACATGTCATTACTTTTGCAAC4140.10854318786623494No Hit
TTTGATGGGTAGCAGGACACAGAAAATGCTTTCCAAGAGTTCGTCAAATTTG4140.10854318786623494No Hit
TAACGCGGGGGAAACTGTGAGCCCAGGAGTTGCCCATCTATGGTTTCATTCC4110.10775664302662455No Hit
GGCTGCGGGATCTATCGCCGGGAGCAGATGATGGATGTCTAGAAAACACCCC4100.10749446141342107No Hit
GCAAATGGGGGGATGAGTCCACTCTGGCCTTCCAGCAGGCCCGCTACAACTT4090.10723227980021761No Hit
AAGCTTGGGTTCATATATACATATATGAGTGCATTTTAATGATGTGCTGATA4090.10723227980021761No Hit
GGGCACGGTTTGTGACAGGTACTTCTGGTTGTGGCTTTGTCCATGGAGACGA4080.10697009818701414No Hit
AAACAAGGGGAGGAGGGGAGACCCGGCACCCACCGGCCCAGAGCTGCCCGAC4050.10618355334740374No Hit
AGTTTTGGGTATCTTGGGGTCTTCCATGTCAGTAGAGAGATATACATCTCTT4020.10539700850779334No Hit
TGATATGGGGTAAGTGAGGACAGGCACTTCTGTTTTTGTGCCCAAATATTAC3960.10382391882857256No Hit
GTTCTTGGGAACCCTACTGAGTAATTGGCTCCATTAAAACAAGAGAGAATTG3950.10356173721536908No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTT3940.10329955560216561No Hit
CATGGTGGGCGATCTCTGGATGGGATTGGAAGTGGATGTAATCTGGATTCTG3920.10277519237575869No Hit
GGCGCAGGGTCAAGAAGGGAGATGTGGTCATTGTGCTGACCGGATGGCGCCC3910.10251301076255523No Hit
TGCCCAGGGGTACATATTTAACGCAAACTTCAGCATCCTTTAAATTTTAAAG3890.10198864753614828No Hit
TCGTGGGGAGCTTAAGAAATGTTGATAAATATTTGAAAATTCAACTAATTAA3860.10120210269653789No Hit
GTTAATGGGAAGCCAGTCACAGAAGAAAAATTTTTGCATGATTCTACTTATA3860.10120210269653789No Hit
CAATGTGGGCTCTGGGATGTGTTCTGACTGTAAAAACTCTGATGTTGTGAAA3850.10093992108333442No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)