FastQCFastQC Report
Sat 31 Dec 2022
SRR5846489.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846489.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences374961
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGC93522.4941260557764675No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGCCGTCT89582.3890484610399483RNA PCR Primer, Index 30 (95% over 22bp)
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCG21760.5803270206768171No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGA16190.4317782382701134No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTCTTATAC11070.2952307039932153No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTCT10940.2917636767557159No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGAACTCGTATGC8550.2280237144663045No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGAT6880.18348574918458188No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATG5500.14668192158651167No Hit
ATGCCTGGGAAAAACACAAATAACATTTAATTTTATTCATTCTGCTTCAAAA5310.1416147279317049No Hit
ATTCAGGGGAACCTCGAAGAGCAAACAGAAAGAAATGGTGATGTGGCAAACC4830.1288133965932457No Hit
TGTGCCGGGAGTGGTAGGAGTTCTTTGTATCTTCTGGATACTAAACCCTTAT4810.12828000778747656No Hit
AGCCTGGGGGACAGAGCGAGACTGTCTCAAAAACAAACAAACAAACAAACAA4770.1272132301759383No Hit
CCGGAAGCTAGAAGCAGATGATTTTTTCCAAAGCTGCGTACTTTTCACTGAG4700.12534636935574633No Hit
AGTTTTGGGTATCTTGGGGTCTTCCATGTCAGTAGAGAGATATACATCTCTT4540.12107925890959327No Hit
GGCTGCGGGATCTATCGCCGGGAGCAGATGATGGATGTCTAGAAAACACCCC4540.12107925890959327No Hit
AACCGAGGGATGGGTCAGAAGACTCAATATCTTAAGATCGGTTTTCTTCAAA4490.11974578689517043No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGT4380.1168121484634402No Hit
ACCATAGGGATACATTATGTCATTTGATGCACTCTCTCAGAAAGCCATTGAA4230.1128117324201717No Hit
TTAACGGGGGGCCGTCTTCTGTCTGCTGTGTGGGAAGCGCTTCCAGGCGCAG4210.11227834361440256No Hit
ACGTGGGGATTACAATTCAAGATGAGATCCGTATCAGGTGCCAATAAAAAAT4200.112011649211518No Hit
TGCCCAGGGGTACATATTTAACGCAAACTTCAGCATCCTTTAAATTTTAAAG4200.112011649211518No Hit
TCATAGGGGCTTCCTGCTAGTTGGCGACCTCATGGCTGTGAACAACTGCGGG4190.11174495480863343No Hit
TAGTTAGGGATCCCCAAGGGCACAACCATAATGGCATTACTGACTTCCGTGC4170.11121156600286429No Hit
TTGTGAGGGCATGTACTATTTCTTAAAACTAACCTACCTGAAAACTGTCTAA4110.10961139958555689No Hit
ACAAATGGGATTACAAAACTGAATGCAAATGTCCTTTGTGGTGCTTGTTCCT4100.10934470518267232No Hit
GCACAAGGGCACAGCACTTTCTCAGACTCACTTATAAGAGTGCGTTTTTATC4100.10934470518267232No Hit
TGCTACGGGCTGATTAAGTTATGTCAAAATGCTGAGCCAGGCATAGCTGAAG4100.10934470518267232No Hit
GCTTATGGGCAGTGAAATTCTGCCTGAAGAAACCTCTTATTGAATTTGAAAA4070.10854462197401862No Hit
ATGTTTGGGAGCCTTTATCTGGTTACAGAGTTTTTTTTCTGTAAAGAAATTA4070.10854462197401862No Hit
GGGCACGGTTTGTGACAGGTACTTCTGGTTGTGGCTTTGTCCATGGAGACGA3980.10614437234805754No Hit
GACATAGGGAACTTATACAATACAATTTTAATTATGTAGAGTCCATGTTTAG3960.1056109835422884No Hit
GCGCGCGGGTAAAGAAAAAGATTGGTCATTTGACTATATAAAAATTTAAAAC3940.10507759473651927No Hit
ATGATAGGGGGACTCACCCACCAAGGTCTTCTGTATGAAGCAGCCAAGGTGT3920.10454420593075013No Hit
TGGCATGGGGGAGGAAATTCAGAAGAAATTAGAAGCTGCAGAAGAAAGACGC3890.10374412272209643No Hit
GTTAGCGGGGGGGAAACTGAAATTTAGACATTTCTTCTAAAATTTCAAAGTA3890.10374412272209643No Hit
AAGCTTGGGTTCATATATACATATATGAGTGCATTTTAATGATGTGCTGATA3860.10294403951344273No Hit
GTTCTTGGGAACCCTACTGAGTAATTGGCTCCATTAAAACAAGAGAGAATTG3850.10267734511055816No Hit
TCCACAGGGGGCATTTGCAGAGTGAGACAAAGCGGAGAACGCTGGTGGGCCT3840.10241065070767359No Hit
GAAAGGGGACAGTATTGTTGTGATGGCCATCTGCTGGAAACTCCTGAAGAGC3840.10241065070767359No Hit
TTGTAGGGACTCTGAACTTTTTACAAAGAAAAATGGAAAAACTTTGTATGGT3820.10187726190190446No Hit
TAGCTGGGGAGGCTGTCTCTGGATGGATTCTGGTGGATGAATGGCAACGCGG3820.10187726190190446No Hit
GCAAATGGGGGGATGAGTCCACTCTGGCCTTCCAGCAGGCCCGCTACAACTT3790.10107717869325075No Hit
ACGCCTGGGATCCAGAAAATATAAATAACTCCTATAAAGTAACAATAAACCA3790.10107717869325075No Hit
ACCCCAGTGTCAAAATCTAGTTCTCAGGTAGCTCTAGGAAACTCCAGTGGGT3790.10107717869325075No Hit
GTTAATGGGAAGCCAGTCACAGAAGAAAAATTTTTGCATGATTCTACTTATA3770.10054378988748162No Hit
TGATATGGGGTAAGTGAGGACAGGCACTTCTGTTTTTGTGCCCAAATATTAC3760.10027709548459705No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)