FastQCFastQC Report
Sat 31 Dec 2022
SRR5846487.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846487.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences197279
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGCCGTCT66353.3632571130226734RNA PCR Primer, Index 30 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGC57962.937971096771577No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCG16290.8257341126019495No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGA11370.576341120950532No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTCTTATAC10220.5180480436336357No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTCT7900.4004480963508534No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATG4650.23570679089005925No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGAT4490.22759644969814322No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGCC4270.21644473055925872No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGAACTCGTATGC3600.1824826768181104No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGT3260.16524820178528885No Hit
ATTCTGGGGGGGACCAAGAATTGTTTGGTATGTTAGAGAAGCACATAACATT2180.11050339873985573No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGACCTCGTATGCCGTCT2050.10391374652142399No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCTGTCTC2050.10391374652142399No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTT2040.10340685019692923No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGACCTCGTATGC1990.10087236857445547No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)