FastQCFastQC Report
Sat 31 Dec 2022
SRR5846486.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846486.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences194862
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGCCGTCT63473.2571768738902405RNA PCR Primer, Index 30 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGC55592.8527881269821718No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCG15370.7887633299463209No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGA11010.5650152415555624No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTCTTATAC9320.47828719811969495No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTCT7180.3684658886801942No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATG4960.2545391097289364No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGAT4690.2406831501267564No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGAACTCGTATGC4640.23811723168190824No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGCC3610.18525931171803636No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGT2940.15087600455707115No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTT2140.10982130943950076No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCTGTCTC2090.10725539099465263No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGACCTCGTATGC2060.10571583992774373No Hit
TTCAGGGGGAATAAAGGAAGATAACTATACTTCATTGATCAATTAATATGGG2010.10314992148289558No Hit
ATTGCGGGGTGATAATTTTGAGATAACTTGTTGTGTATGAAAATGGAACTCA1970.10109718672701706No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)