FastQCFastQC Report
Sat 31 Dec 2022
SRR5846485.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846485.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129143
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTACATCAATCTCGTATGCCGTCT8900.689158529691892No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTACATCAATCTCGTATGC6040.4676985976785424No Hit
CACCAAGGACGCTCCATTATGGATGGTAGAAATGCACTAAGAATTAGTGAAA1980.15331841447078046No Hit
CGATATGGGCGATCTTGCAAGGGAAGCATTAGGACTGATACAAATAATATAT1920.14867240191105982No Hit
TTGCAGGGGAGGGATGCAAGACTGGTTCAATACTCAAAAATCAATGTAATAT1840.142477718498099No Hit
AATTCCGGGGACATTATATCTTAGATGTGTAATTTATGAACTACTACTGGAT1690.13086268709879745No Hit
AATTTGGGGCTCTTTTTAAAAAACTCTTTGGTGCACAAGTAACACATTTGGC1680.13008835167217736No Hit
AAATGTAGGGTCCCATGAAGCTGAGGTCTTGAAGCAGCTGGCTGAGAAACGA1610.12466800368583664No Hit
TAAACCCGGGTCTCAGTCTGTGGAGAGAACAAAACCCAAGGCAAAGATAGCA1560.12079632655273612No Hit
TAAAAAGGGAATGGAAATCATAACAAACAATCTCTCAGACCACAGTGCAATC1540.11924765569949591No Hit
ACAATCGGGAACAAATAGGTGTGGTTATAAATGGGGAAGAAGTTCTTTGATG1510.1169246494196356No Hit
GAGTTTGGGAATATCACTTATGAAATCCTATTGGTGGATATCGTAGTCTCCT1490.11537597856639539No Hit
ATTTGGGGGAGGCTGTTTATTTCAACCTTTTTAAAAATTGTGTTTTTTAGTA1490.11537597856639539No Hit
ACGTTAGGGATGGCAGCCTAGATAAGTAGAAATGGCTTGGATACTGAATCAT1490.11537597856639539No Hit
TCCTAAGGGGATAAGAGTGATGCTGTCTCACACACAGGAAACTACACTTGTG1490.11537597856639539No Hit
GCGAGGGGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCAT1480.11460164313977529No Hit
ACAGTTGGGTATCCTCCCAACTTGCAGATCATTCCTTTTCCCCTCAGATGAC1480.11460164313977529No Hit
AGCAATGGGCCGCAAGATGGAGTACAAGGGCAGCAGCTGGCATGAGACCTGC1480.11460164313977529No Hit
GTTTTGGGAGTCAACAGAAGGAAAAGGCTCTTTTCCTTGGAGATTTTCAAGT1470.11382730771315518No Hit
TACACAGGGATTTCTGGTGGATTGTGCAGACCACGAAAGGCTGTTAGAGTCA1460.11305297228653508No Hit
CTTGTCGGGGGGGGGACAGGCGCGCCCGCGCCGCCCGCTCCAGCTGGGCTAG1450.11227863685991499No Hit
TTTTCAGGGTGGGAGGGGGATGTTGGGAGGCATGGGGGGTGATTAAATTATC1440.11150430143329487No Hit
GTGTGCGGGGAGAACCCTGAGGAAGTTTACTGTCATGAGTCTCGGCTGGTTG1440.11150430143329487No Hit
ACATAGGGATTCTTCTTCCTATTGTTCCCTGACATCCAGCAAATTGTGAATT1420.10995563058005466No Hit
CCTTCAGGGCAAGTAACACATTTGGCCACAAAACACCAAAGAATTGTAGGCA1410.10918129515343457No Hit
GCAATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1410.10918129515343457No Hit
ATTAGCGGGAACTGACTTGCGCATTCCAGCTTCCGTTTGCACTATGTTTAAT1410.10918129515343457No Hit
GTACCAGGGCAAGTGGACATGTGTGTATTTACTAAACTCTGTGGACCTGGAA1410.10918129515343457No Hit
CACAGGGCTTCCCTCTCTCGGGGTTGGCTTCTTCCCAGGCCTTGCATGGGCC1410.10918129515343457No Hit
CCTGATGGGCAGGGTTCAAGCGAGTCTCATGCCGCAGCCTCCCAGGTAGCTG1410.10918129515343457No Hit
TGTCCGGGGGTGTGACAATCAGGATATAGAAAAACAAACGTAGTGTTGGGAT1400.10840695972681447No Hit
GCAATAGGGTCAGTATTGTGCTGAAAGAAACATTGATGTGAACCCAGTGATC1390.10763262430019435No Hit
AATGCCTGGGATAGATCAGAATTTTAAATTAAAGGTTTTTTCTTTAAATGAT1380.10685828887357425No Hit
GCCTAGGGAAGAATGCGCATGACGCTCTGTGAAGGCTGGAACTCAGGTCTTC1380.10685828887357425No Hit
TTTCGAGGGGAGAATGATAGAAGTACTAAAATCTGTCAAGTGTTTTCAGTAT1370.10608395344695416No Hit
TTCTTAGGGATCTCCAGAGAGGACATGCAGATAGCCAGTGAGCACAGGAAAA1370.10608395344695416No Hit
GCTTCCGGGCCAAAAACTGCACCAGAAACAGCTGCAAAATTTCTTAAGGAAG1360.10530961802033405No Hit
CAACTGGGGAAGGAATAAAAGTTCTTGAAACACTGGTTGCTCTTGGTGAAGA1360.10530961802033405No Hit
TTTACTGGGCTGGAGATTTTATATAAATGAAACCATAAAATAAGTGGTCTCT1360.10530961802033405No Hit
GGATCAGGGATGATTAATGAAGTAGATGCTGATGGTAATGGCACAATTGACT1350.10453528259371395No Hit
TATTGAGGGCAAGTCACTAACCTTCCTCTTTGATCTCTTTGTAGGCTGACCA1350.10453528259371395No Hit
ATGTTCGGGATCTCAGGCGGCAGTGAAGTCCAGATAGTTTTGCAGATTGTTT1350.10453528259371395No Hit
CTCACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG1340.10376094716709386No Hit
CCGCATCCACTTCCCTCTGGCCACATATGCCCCTGTCATCTCTGCTGAGAAA1340.10376094716709386No Hit
AGACCAGGGATCTTTTGAAGTGTGTTTGGGATGCTGATATTCAGTAAAGCAA1330.10298661174047373No Hit
AGCTACGGGATCTAACCAGCATAATCATGTAGGTAGAAAAGTGATTATAGAT1330.10298661174047373No Hit
AAACAGGGAATGTAAGCAAAGAGATAAAAACACTAAGAAGAAATAAAGATGA1320.10221227631385364No Hit
CAATAAGGGCAGCATGAATCGGGTTATTGAAGAAGATGGAACCAGTGCTTAA1320.10221227631385364No Hit
TTTGTTGGGGAGTTCAAAATGGCGACGGCGGCGGCAGCGGCGGCGGTGATGG1320.10221227631385364No Hit
TGAACAGGGGAATGTTTTTATCTTGCAAAGCTAAATCTCACTACACATTAAA1320.10221227631385364No Hit
TAGACATGGGATGATCCATATAGCTTAGAATGGATGTTTTGGATAAATGTAT1320.10221227631385364No Hit
TGAGTTGGGATCCATGGGGACTAGCAGTAATGCACAAGTACATAAAAGCACT1310.10143794088723353No Hit
TGCCTAGGGGTTTTCAAAAATGATTTGGTGGCATTGGAAAATCTTAGTGACA1300.10066360546061343No Hit
CTCTATGGGGATCAGTTACTGTCTCTCCAGCTCAGATTATTTGTAAAAGATG1300.10066360546061343No Hit
GCTGCGGGGAGATTGTTGGTCCACTGGGCATATTGAACCATCCACATTGCTT1300.10066360546061343No Hit
CTTATATAGGGAAGGAAGAATAAAAAAGAAGAGGTTCATTTTTCTGGCTTTT1300.10066360546061343No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)