FastQCFastQC Report
Sat 31 Dec 2022
SRR5846483.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846483.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127483
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTACATCAATCTCGTATGCCGTCT8540.6698932406673831No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTACATCAATCTCGTATGC5940.4659444788716927No Hit
CACCAAGGACGCTCCATTATGGATGGTAGAAATGCACTAAGAATTAGTGAAA2040.1600213361781571No Hit
AAATGTAGGGTCCCATGAAGCTGAGGTCTTGAAGCAGCTGGCTGAGAAACGA1840.1443329698861809No Hit
CGATATGGGCGATCTTGCAAGGGAAGCATTAGGACTGATACAAATAATATAT1800.14119529662778568No Hit
TAAAAAGGGAATGGAAATCATAACAAACAATCTCTCAGACCACAGTGCAATC1760.13805762336939043No Hit
TTGCAGGGGAGGGATGCAAGACTGGTTCAATACTCAAAAATCAATGTAATAT1750.1372732050547916No Hit
CTAACCGGGTGGAGAGTACCTTGTTATATATACAGATACTTTCATGGTCATT1570.12315367539201307No Hit
AATTCCGGGGACATTATATCTTAGATGTGTAATTTATGAACTACTACTGGAT1530.12001600213361781No Hit
CTTGTCGGGGGGGGGACAGGCGCGCCCGCGCCGCCCGCTCCAGCTGGGCTAG1500.11766274718982138No Hit
GCCAAGGGTAAGCAAAAGTGATTACGGGCAGGGACGACCTGTCAAAGCGCGG1500.11766274718982138No Hit
CCTGATGGGCAGGGTTCAAGCGAGTCTCATGCCGCAGCCTCCCAGGTAGCTG1500.11766274718982138No Hit
GTGTGCGGGGAGAACCCTGAGGAAGTTTACTGTCATGAGTCTCGGCTGGTTG1490.11687832887522258No Hit
CAGGAAGGGGAGTCAAATGAAGGACATTGAAAAAATGTACAAAAGTGGATAC1450.11374065561682734No Hit
TTAACGGGGTTATCTTATTTGATATGAGAGGGGCCCATAGAAGAATGTGAAT1450.11374065561682734No Hit
ATTAGCGGGAACTGACTTGCGCATTCCAGCTTCCGTTTGCACTATGTTTAAT1450.11374065561682734No Hit
GTCTTTGGGGTAGACTATTGAAGAATGATCTCTTTGTGATTTAAGAAGTGGC1420.11138740067303092No Hit
TACACAGGGATTTCTGGTGGATTGTGCAGACCACGAAAGGCTGTTAGAGTCA1400.1098185640438333No Hit
TTTCGAGGGGAGAATGATAGAAGTACTAAAATCTGTCAAGTGTTTTCAGTAT1390.10903414572923449No Hit
ATGTTCGGGATCTCAGGCGGCAGTGAAGTCCAGATAGTTTTGCAGATTGTTT1390.10903414572923449No Hit
TGGGTAGGGTTTTAGTCAATTGAATAATAAATTATCCTGTCTTTTCTGAATC1390.10903414572923449No Hit
GCGAGGGGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCAT1380.10824972741463569No Hit
GGCCTTGGGATGTGTAGCTCAAAACTATTTGTGATCTACTGTTTGCATGTAA1370.10746530910003688No Hit
AAACAGGGAATGTAAGCAAAGAGATAAAAACACTAAGAAGAAATAAAGATGA1360.10668089078543806No Hit
GCTTCCGGGCCAAAAACTGCACCAGAAACAGCTGCAAAATTTCTTAAGGAAG1350.10589647247083923No Hit
CCGCATCCACTTCCCTCTGGCCACATATGCCCCTGTCATCTCTGCTGAGAAA1340.10511205415624043No Hit
TTGCGCGGGAGCTATACTCCATCTAAAATAAGACATGCCTGAAATTTGGCAA1340.10511205415624043No Hit
ATAACAGGGGTATTTTCTCAAGATTAGTTATTGAAGCTCAGCAGTTTTTGTG1330.10432763584164162No Hit
TTGTCAGGGAAGGGAAAGGGAGATTGGAAATGTAGAAGCAAATTATTTTTAA1330.10432763584164162No Hit
AACAATGGGTACACCTCTGGATGAGAAGAGCCTTGCTTTATTACTCAATTAT1320.10354321752704282No Hit
TGTCCGGGGGTGTGACAATCAGGATATAGAAAAACAAACGTAGTGTTGGGAT1320.10354321752704282No Hit
ATTTGGGGGAGGCTGTTTATTTCAACCTTTTTAAAAATTGTGTTTTTTAGTA1320.10354321752704282No Hit
TGTCGAGGCTATTGATAACTGAGCAAAGAGCGAAGATCCAGAAATACGAAAA1320.10354321752704282No Hit
GCTAGAGGGATAATATTCACATAAACTATAAATACTAAAAGTATAAACATAA1310.102758799212444No Hit
AATGCCTGGGATAGATCAGAATTTTAAATTAAAGGTTTTTTCTTTAAATGAT1310.102758799212444No Hit
TGCCTAGGGGTTTTCAAAAATGATTTGGTGGCATTGGAAAATCTTAGTGACA1310.102758799212444No Hit
GGATCAGGGATGATTAATGAAGTAGATGCTGATGGTAATGGCACAATTGACT1300.10197438089784519No Hit
ACGTTAGGGATGGCAGCCTAGATAAGTAGAAATGGCTTGGATACTGAATCAT1300.10197438089784519No Hit
TAAACCCGGGTCTCAGTCTGTGGAGAGAACAAAACCCAAGGCAAAGATAGCA1300.10197438089784519No Hit
TTCGGGGGAAGCCCATCACACCTGGCCTCCACTTTTGGAATATTATGAATAT1300.10197438089784519No Hit
GTATTGGGGACACACAGTATATTAAACGAAAACGTAAATAAGGTATAAATAG1300.10197438089784519No Hit
AGGTAGGGCTGATTATTCAAATAGATGGATTTTCAGGACCAACAACAAACCA1300.10197438089784519No Hit
TGAGTTGGGATCCATGGGGACTAGCAGTAATGCACAAGTACATAAAAGCACT1290.10118996258324639No Hit
TTTTCAGGGTGGGAGGGGGATGTTGGGAGGCATGGGGGGTGATTAAATTATC1290.10118996258324639No Hit
TCCTAAGGGGATAAGAGTGATGCTGTCTCACACACAGGAAACTACACTTGTG1290.10118996258324639No Hit
GCAATAGGGTCAGTATTGTGCTGAAAGAAACATTGATGTGAACCCAGTGATC1290.10118996258324639No Hit
TTATGAGGGGGATAGAAAAGATTGTATTATCATTTGGGATTTAAGGTAATAA1280.10040554426864758No Hit
TCTAATGGGATATCTTGGTGTTTGTAAGTAAGTTGCATGATAAACACAAATG1280.10040554426864758No Hit
TTTACTGGGCTGGAGATTTTATATAAATGAAACCATAAAATAAGTGGTCTCT1280.10040554426864758No Hit
TGTTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1280.10040554426864758No Hit
GCGCCAGGGGGAGACTCGTCACCAGGCGTGCAGTGGGCACTGCTGGGCTCCC1280.10040554426864758No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)