FastQCFastQC Report
Sat 31 Dec 2022
SRR5846482.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846482.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31220
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTACATCAATCTCGTATGCCGTCT4721.5118513773222293No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTACATCAATCTCGTATGC4081.3068545803971814No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTACATCA980.3139013452914798No Hit
AGAACTGGGAGTGGGAAACTTTAACATCCAACTGTCAAAATTAGACAGATCA800.25624599615631005No Hit
CTTATACACATCTCCGAGCCCACGAGACCTACATCAATCCTGTCTCTTATAC730.2338244714926329No Hit
AGGACCGGGGGTTGACGCGGCCTGGGCAGATCCTTGCAGAAAGCTGCAGGCT720.23062139654067906No Hit
GCTCGGGGGGTGCGGCGCCGTCGATGGTGACGAATTGCGCATAGTCGACGCC710.22741832158872516No Hit
AGAAATGTGGAGATCAGATTAGAAAAAAGAAAATCAAAACAAAAAAGACAAT700.2242152466367713No Hit
TGCACTGGGATATATTTCGGGGTAGTGTGAGCTTTTAATGTTTAAGTCATAT650.2081998718770019No Hit
GCAGCTGAACCCCACGCAGGCCTTCTTCCTGCTGGTGAACCAGCACAGCATG640.20499679692504805No Hit
CAAGCCGGGTCAGCAGGAAGTATTGTCTGTGTGTGATGACGGGGCAGTATTG560.17937219730941703No Hit
GGTTCAGGGCTGAAGAAGAAAGGTGGCAAATACAGTGTTCAGTTCAAGTTGC560.17937219730941703No Hit
CGCAAAGGGCTGTTAGCGGGGATCTATCCCTGGGCTGAAATAAAAGAGGAAA560.17937219730941703No Hit
GAAGATGGGACGAGAGCCAGCAAGCAAAATAGATGTGGCTGGGTCTGCCTGT550.17616912235746315No Hit
GCAGTTGGGCAACCCGGGACAATCAAAGGTATATAGCTACATACGCATGTAT550.17616912235746315No Hit
ATACCGGGGTCAATGGATTTGCAATAACAATCCAAAGATGGAAGAAGACACT550.17616912235746315No Hit
TATGCTGGGACCATCTTTGCCCCCAGGATACAATTCTTTGCCATTGAAATTG550.17616912235746315No Hit
TCGACTGGGCAGACTCCATTGGGCACATCTGGGAGGAAGGCAACCTCCTTTG540.1729660474055093No Hit
GCAAATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA540.1729660474055093No Hit
AACTTAGGGCAGGATGTAGGAGATGACAGTGCTTTATGAGCCTTAAATTCGG540.1729660474055093No Hit
GGCTCGGGGCCCAAGGAGGGGGCCTCATTGTGTCTCCCGTGCATGCTCTGCA510.16335682254964767No Hit
AATCTTGGGACATCCAGATTATCTGGAATAAGCTCCCTCACTTAAGGTCACT500.1601537475976938No Hit
AGCTAGGGGGTGGGGCAGGTAGCTATCCCTCCCATGTCATTAGTAATCCTTT500.1601537475976938No Hit
GGGCGGGGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAA490.1569506726457399No Hit
GTAATAGGGATGTGCTGTTTGGTTTTCTGTTCCTGTGTGTGTTTGCTGAGAA490.1569506726457399No Hit
GCTTAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA490.1569506726457399No Hit
GCTGCCCGGGAGTTAACATCGCTTTAGCTCTTGTCACTATTTCATTTTGTCA490.1569506726457399No Hit
CGTCAGGGGGAAGCTTCTTCATGGAGTAAAAAATGTATTTAAAAGAAAATTG480.15374759769378604No Hit
TGTAAAGGGTGCCGGGGATGCTAGGCACACTGCCCCCTTGAATAGACATTAT480.15374759769378604No Hit
CTAGTTGTGGGAGGAAAGGGTTAACTCAGCAGGTCTTGTTTGCCCAGACTCT480.15374759769378604No Hit
TGTATGCGGGGCGAGCACAACTCAGGTAGAAATGAGGATGTCATCTTCCTTC480.15374759769378604No Hit
GTACTGGGGTACCTGAGGCTGTTGGTCAAGTTATACAGGTTTCTGGCCAGAA480.15374759769378604No Hit
CGAGTCGGGCTACCTAATTACCTCCCAAAAGACCCTACCTTCTAATACCATC480.15374759769378604No Hit
ATTGCAGGGCTTGGGAGTATCACAGGAAAATCACAATTACACCACTTTAGAC470.15054452274183217No Hit
TAGCACGGGCGACGCTCTTCCGATCTTAAATGGGGTAAAGGCTACAAATAAA470.15054452274183217No Hit
CCGTAGGGGAAACTTCATCCAATGGTTTCCTAAATGGTAGCTCTAAACATGA470.15054452274183217No Hit
AGGAGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC470.15054452274183217No Hit
TAAGCTGGGAATATTTAGTGGGTTTGTTTTGGTTGGTTGGTTGGTTGGTTGG460.1473414477898783No Hit
TTCATTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC460.1473414477898783No Hit
TTCTTTGGGGTTCTAGGTCCCAATTCTACTGCAAATAGTATCATGGCATCTT460.1473414477898783No Hit
GTGATAGGGAACAGTCGACACAGAGGTCTTTGAGTTGGATGGTTGCAAATAT460.1473414477898783No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTACATCAATCCTGTCTCT450.1441383728379244No Hit
GAAGCAGGGATCATTGTGTAATTTGTAAAGATTCACGTGTAGTTCTTTATTA440.14093529788597053No Hit
GGTCTTGGGATCTGTGGTTTGTGTCTCACTTTAAGGCTCAACTGCATCTTGG440.14093529788597053No Hit
GTAGCTGGGAATAATAAAAACAATAGTATGGAAATAATAAAAACTTAAAAGG440.14093529788597053No Hit
TGTTGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA440.14093529788597053No Hit
CCAATAGGGATGTTTCCAAATCTGTGTATTGTGATTTTTACATACTAATGAA430.13773222293401666No Hit
ACACTGGGGTCCAGAATTCATGAGGAACTTAAAAAACTCAACAACAAAAATC430.13773222293401666No Hit
TGCTGTCGGGGTGTTTTAGATCACCTGAGAACATTTCTTGAACACTAGTATT430.13773222293401666No Hit
CTTCATGGGAAACTTATTCCATTTATAATGTTCTCTTTATATTGGTTATGCT420.13452914798206278No Hit
TTACGTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA420.13452914798206278No Hit
GAGTACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC420.13452914798206278No Hit
AGTTTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC420.13452914798206278No Hit
GCTGCAGGGCGATCTTACGTTGGGACTCAGGGGCTTCCAGGAAAATGCCAGC420.13452914798206278No Hit
GCCTGGGGACTTGTTTTTTACATTTTAAGAGTTGTAGAAGTTGTTCCTAACT420.13452914798206278No Hit
AGGCATGGGCAGACATTCCATATAAATGGAATAACTGATTTTTTTACACAAA410.1313260730301089No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTACATCAA410.1313260730301089No Hit
GAAACTGGGCAAGCCCAAGAAGAAAGTCTTCGAGAAGTTGCAGGCTGACTTC410.1313260730301089No Hit
GTTGCGGGAAGTGGGTAACATTTTATGCAGTTTAAATGAATGGAATATTTTC400.12812299807815503No Hit
GTACGGGGGGTACGTGAATGTTCACAGCAGCATTATTCACAATGATTAAAAG400.12812299807815503No Hit
GAGCGAAGGGCAAGCTTTGCTGTGAATAAAAGGCTTACATTTGTTCCTGCAT400.12812299807815503No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTACATCAATCTCGT400.12812299807815503No Hit
GGTTATGGGGAAGAAGCCAGATCCAGCTTCCCTGCGGGCTGCTTCTTGTGGG400.12812299807815503No Hit
TTTGAAGGGGAGGCATTGAGGCAGCCAGCGCAAGGGCTTCTGCTGAGGGGGC390.12491992312620116No Hit
GTATAAGGGCAATGTCAGTACTGAAACTCCTACTCTCCCCTCCCGCCCCACT390.12491992312620116No Hit
TCGCAGGGGGATCACATTTCAGAGATGCTAAAATGTGATGTCACAGGTGTCT390.12491992312620116No Hit
AGAGAAGGGGATAGAACTTTCTGGCAAAGGTGTATACATTTGTCACAGCTCA390.12491992312620116No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTACATCAAACTCGTATGC390.12491992312620116No Hit
CATAATGGGACAGTGGTTACATAATACACTGGCTCACTGCAGACTCTCTTGT390.12491992312620116No Hit
GACCTTGGGACGAAACATCTGTGCAGTTTTCAGAGTGTCACAAAGTCAATAG390.12491992312620116No Hit
ACAACAGGGGGCCGGAGGAACTGTGCTCTTTGAGGCCGACGCTAGGGGCCCG390.12491992312620116No Hit
TTGCCAGGGAGATAACTGATTATAGGAGTTGAAGTTCAGGAGTGAAGTCTGA390.12491992312620116No Hit
TGGCCTGGGCATAATAAAGGAACTTTCAGAGATTGGTTGAAACCCAGTGGTA390.12491992312620116No Hit
TTCCGGGCACCCTTTTGTATCCACCCTTTCCAGCTACATTACTTTAGACAGT390.12491992312620116No Hit
TGATGAGGGCTCTAATAAATTTAACACAGAGAATAGTTTCCCCATTCACTTT380.12171684817424727No Hit
CTTTCTGGGCAGTGGCTAACCTGGTTAGAAACTGCTGAAGAAGAAGAATCAG380.12171684817424727No Hit
GACACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA380.12171684817424727No Hit
ACTGTAGGGACTATTTATTATAAACTCTATATTACAAATAAAGAATTGAGCC380.12171684817424727No Hit
CCTACTGGGGAATAGAACGTGAAAGCCAGCTCTTTCAGAATATCCTCTATTA380.12171684817424727No Hit
TGAATAGGGGATCTTCGGCTGGGAAGTAGAATCAATGAAGGAGGAGATAGAG380.12171684817424727No Hit
ATTCCAGGGATAAGAAAGGACCTATAGTTATATTTATTTTGCATTTGTTCTT380.12171684817424727No Hit
TTAATGGGGATGTAAAATGCTTTTTAACAGCTGATACTGTATAAGACAAAGC380.12171684817424727No Hit
AGGCATGGGGGAATTAACTTGCAAGTTCTAAGTTCATATATAAAATTCAATT380.12171684817424727No Hit
CTTCTAGGGATGCCCAAGGCCTAGGTTATGGGTACGGCAACCACATGTCCCA380.12171684817424727No Hit
TTATGAGGGATACTACAGATGGTAAGTATTTAAGCCTGTAAGTAAGTAATTA370.1185137732222934No Hit
AAGAATAGGGCTCTTATTCTCTTAAAACCCAGCCAAGGAAAACTAAAACTAT370.1185137732222934No Hit
TACCTAGGGACAGTCTAAAAGTCTTGATGGTAACTATAGTGTAATTATCTTT370.1185137732222934No Hit
ACAGCAGGGGGGAATGGGAGTGCCTGGACACTACCCAGAGGAAATTGTACAG370.1185137732222934No Hit
CAGTAAGGGACGACTTCGCGCGCTACCGGAGCACAGTGCACGGGGTGACCCA370.1185137732222934No Hit
TAACGCGGGAGGTCGTCCTCTCTGTATGTGCCCCGCGCATTTCCTTGGCCTC370.1185137732222934No Hit
TCACATGGCAAACAACTAGTAAGAAGCAAAACCATCATAAATAAGAGTTCTC370.1185137732222934No Hit
ATGCGTGGGGCTGGGGATGGTGGGAGCCTGCGCAGCTGGCTGGGCGGTCTCT370.1185137732222934No Hit
ACTAGCGGGGTGTCCCAGCTTTGTCACTTCTAGAACAAGACCATGAGTACAA370.1185137732222934No Hit
GTTTTAGGGGATTTGGAAATAGGATGTTTTCAACGTTCTTTTGTCTTTTGCT370.1185137732222934No Hit
AACTATGGGGCAGAAGAGGGACCCCAAAATGTAAATGGATGTGGACAAAAAC360.11531069827033953No Hit
AAGTGAGGGGACGCTCTTCCGATCTTCCCTGGGCAAACTCCTGACCTCGGGA360.11531069827033953No Hit
GGTTCTGGGACCTCTAAGAGAACTTTCTGAATTTGCAGCTAATCTCGGGGAA360.11531069827033953No Hit
AATGCTGGCTAGACGAGGGGAAGTAGGAGGATGATATCTACCATCATGAAAC360.11531069827033953No Hit
TGCTTAGGGACCCCTGAGACTGAATTTGCCCTGCTAATGAACTGAGGCATAT350.11210762331838565No Hit
TATTGCGGGTTATGGATACTAGGCTTGATATATGGGTGATGACATGATCTGT350.11210762331838565No Hit
GTTCGCGGCTGATGTTTTTATTGAACATTGTGTTCATGCTTTTGAAGAATTA350.11210762331838565No Hit
TCTACAGGGCAGCGAGGTGGTGCTGACAGTGTGGACTTGAGCACACTTATGC350.11210762331838565No Hit
AGCTCAGGGCCACACCAGAGCTAGAGTTTTCTTTCTACAATCCAACTGATTC350.11210762331838565No Hit
AACAGGGGGAAAGAGTTTCAGGGGCTCATAATTTTGAGAGATGGAGGAAGTT350.11210762331838565No Hit
AGCTGAGGGTGTGTACTGAATAATGTAGGCAGTTGTAATGTAACACCTAGTG350.11210762331838565No Hit
ATAAAAGGGATTCAAGAAGATAGTTGTAAAGATATTGTATCTCCTTTAATAT350.11210762331838565No Hit
TGAGTTGGGAGAACCCATGTCTCTAAACTGCTAGATCAGCCTGCGTTATTTG340.10890454836643176No Hit
CGCTTTGGGTCCTTACAGCCCTGCTGCTGGGGTTGATTTTGAACTGGAGTCA340.10890454836643176No Hit
GCTCATGGGGTGTCATGATTTCAATGATTGTTTAATTGTAGGTCAATGAAAT340.10890454836643176No Hit
TCCTGAGGGAGATGTTGAATAAATGAATCACCTCTTTATAGTGCCTGTGCCC340.10890454836643176No Hit
TAGCCCGGGCTCCTTTTCCTCCTTCTCCTCAACCTTTTCAATGTGAAGACGA340.10890454836643176No Hit
ACACAAGGGGATCTGATCAGGGGATGTTTTGCATAGAGACAACCACAAAGCA340.10890454836643176No Hit
GCACATGGGACTAAGATGTTTATTGAAGACAGTTAATTTAATTGCATTCATA340.10890454836643176No Hit
CTGTATGGGGCCATAAAGAAGGTGAGAAGAGCAAAAATACTGCTGCTATTAA340.10890454836643176No Hit
TGCAGTGGGACCAAAATCAATTCTTGATTGTACACAGACAGCATTGGTTCTA340.10890454836643176No Hit
GGGCTGGGGGTGTAACTTTTCAATTTTGCTCATTTGAATTGGGTCTGTCATA340.10890454836643176No Hit
CCCCCAGCCCCATGATCGAAAGCACCCCCATGTCCATTGCTGGCGATGCCAC340.10890454836643176No Hit
TATCGCGGGACGACGCCTGGCTGATTTTTTGTATTTTTGGTACAGACGGGAT340.10890454836643176No Hit
TTGTGCGGGATAGCCTCTCAATGAAGCGTTGGTTTTCTCATGTCTGGAATGA340.10890454836643176No Hit
TAGATTGGGATGATCAATGGGCTTTGGACCATGTGGAGGTCGTCCTAGTAAG340.10890454836643176No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTACATCAATCTCGT330.1057014734144779No Hit
CGTAATGGGAATAAATAAAAAGAAAGAAAGAAATGATGTAGTGAAAATATAG330.1057014734144779No Hit
CTATATGGGGGTTTTTAATTTTGTGTTGCTAAAAAAAGTGCATGATGGTGAG330.1057014734144779No Hit
TTACGCTGGGCCAGGAAAAGCCTTGCTTGCCTGTTTCCCAAAGAGCTCTAAG330.1057014734144779No Hit
GTTCTAGGGACACCCTGGCTGACCTGAAGCGCCAGCGAGCTGCTGCCAAGCT330.1057014734144779No Hit
TCTACAGGGGGATATAATCAGGATGCATTTATCTTATGAATCTCTGGACATT330.1057014734144779No Hit
CATTTAGGGACTACAATGAGGCTGCTGGTAACAAATATGTACCTCGGGCCAT330.1057014734144779No Hit
AGCAGCGGGTAACTGTATACTGGATGTACATTTTCCCATGGTTGTCTACAGG330.1057014734144779No Hit
ATGCACGGGAGATCCTGTGATGGCGAGACAAATGATCCTTAAAGAAGGTGTG330.1057014734144779No Hit
GTCAAGGGGGATTTGCAAAATGCAGATTATATTTGATAGGCTATAGTATGTA330.1057014734144779No Hit
ACGTAGGGCTTTTCATTTAATTAACTCTTTTTGAACTCCTTAGCAATATTTT330.1057014734144779No Hit
GGGCGGGGGCATGTAGGCCTTTCTACCCGTTTTCCTGTAGCATTAAACCTGC330.1057014734144779No Hit
GCTCACGGGATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAG320.10249839846252402No Hit
TTAAAAGGGCAACTTTTTACACATGTGGGCCTGCAAATATCTATGCAGATTT320.10249839846252402No Hit
AGGCGCGGGAACATCATACAACTCCTTTGACCCTACACCCGACCCCACCTCT320.10249839846252402No Hit
TGTTTGGGGAGGACCGGTGGCAGTTGGTTTAAGATCCTTCTATTCTACGTAA320.10249839846252402No Hit
AAACAAGGGATTGCTCAGGATCGCAATATTGCCATTCAGAGCCAATTTACAA320.10249839846252402No Hit
CAGCAAGGGCAGGCCATCAGCATCCGGGACCATATGAATGCCAGTGCCCAGG320.10249839846252402No Hit
CAATTAGGGCATTTTCCTGCCTGGTTGCTGAGACTCCATTTTGCTGCCACAC320.10249839846252402No Hit
ATACATGGGTAGTGAAGCATTTATTGCATGGATTCACGTGAAAGCATTACGG320.10249839846252402No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)