FastQCFastQC Report
Sat 31 Dec 2022
SRR5846481.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846481.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30125
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTACATCAATCTCGTATGCCGTCT5221.732780082987552No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTACATCAATCTCGTATGC4421.467219917012448No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTACATCA1050.34854771784232363No Hit
GCTCGGGGGGTGCGGCGCCGTCGATGGTGACGAATTGCGCATAGTCGACGCC750.24896265560165973No Hit
CTTATACACATCTCCGAGCCCACGAGACCTACATCAATCCTGTCTCTTATAC730.24232365145228216No Hit
GCAAATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA720.23900414937759337No Hit
ATACCGGGGTCAATGGATTTGCAATAACAATCCAAAGATGGAAGAAGACACT700.23236514522821577No Hit
AGGACCGGGGGTTGACGCGGCCTGGGCAGATCCTTGCAGAAAGCTGCAGGCT680.22572614107883815No Hit
TATGCTGGGACCATCTTTGCCCCCAGGATACAATTCTTTGCCATTGAAATTG630.2091286307053942No Hit
CGAGTCGGGCTACCTAATTACCTCCCAAAAGACCCTACCTTCTAATACCATC630.2091286307053942No Hit
GCAGTTGGGCAACCCGGGACAATCAAAGGTATATAGCTACATACGCATGTAT600.1991701244813278No Hit
GCTTAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA600.1991701244813278No Hit
CGTCAGGGGGAAGCTTCTTCATGGAGTAAAAAATGTATTTAAAAGAAAATTG590.195850622406639No Hit
GGTTCAGGGCTGAAGAAGAAAGGTGGCAAATACAGTGTTCAGTTCAAGTTGC590.195850622406639No Hit
TGCACTGGGATATATTTCGGGGTAGTGTGAGCTTTTAATGTTTAAGTCATAT590.195850622406639No Hit
AGAACTGGGAGTGGGAAACTTTAACATCCAACTGTCAAAATTAGACAGATCA550.18257261410788383No Hit
AGGAGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC550.18257261410788383No Hit
CAAGCCGGGTCAGCAGGAAGTATTGTCTGTGTGTGATGACGGGGCAGTATTG540.17925311203319502No Hit
CAGTAAGGGACGACTTCGCGCGCTACCGGAGCACAGTGCACGGGGTGACCCA540.17925311203319502No Hit
TGTATGCGGGGCGAGCACAACTCAGGTAGAAATGAGGATGTCATCTTCCTTC540.17925311203319502No Hit
CCAATAGGGATGTTTCCAAATCTGTGTATTGTGATTTTTACATACTAATGAA520.17261410788381742No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTACATCAATCCTGTCTCT520.17261410788381742No Hit
GCAGCTGAACCCCACGCAGGCCTTCTTCCTGCTGGTGAACCAGCACAGCATG520.17261410788381742No Hit
ATTCCAGGGATAAGAAAGGACCTATAGTTATATTTATTTTGCATTTGTTCTT520.17261410788381742No Hit
TTTGAAGGGGAGGCATTGAGGCAGCCAGCGCAAGGGCTTCTGCTGAGGGGGC510.1692946058091286No Hit
CGCAAAGGGCTGTTAGCGGGGATCTATCCCTGGGCTGAAATAAAAGAGGAAA510.1692946058091286No Hit
AATCTTGGGACATCCAGATTATCTGGAATAAGCTCCCTCACTTAAGGTCACT500.16597510373443983No Hit
TCGACTGGGCAGACTCCATTGGGCACATCTGGGAGGAAGGCAACCTCCTTTG490.16265560165975104No Hit
AGAAATGTGGAGATCAGATTAGAAAAAAGAAAATCAAAACAAAAAAGACAAT490.16265560165975104No Hit
GAAGCAGGGATCATTGTGTAATTTGTAAAGATTCACGTGTAGTTCTTTATTA480.15933609958506226No Hit
ACACTGGGGTCCAGAATTCATGAGGAACTTAAAAAACTCAACAACAAAAATC480.15933609958506226No Hit
GCTGCCCGGGAGTTAACATCGCTTTAGCTCTTGTCACTATTTCATTTTGTCA480.15933609958506226No Hit
CGCGCTGGGCATTAACCTTTCCACCTTAAGACACTAGAAAGAGAACAAACTA480.15933609958506226No Hit
CTAGTTGTGGGAGGAAAGGGTTAACTCAGCAGGTCTTGTTTGCCCAGACTCT470.15601659751037344No Hit
ATGCGTGGGGCTGGGGATGGTGGGAGCCTGCGCAGCTGGCTGGGCGGTCTCT470.15601659751037344No Hit
GAAGATGGGACGAGAGCCAGCAAGCAAAATAGATGTGGCTGGGTCTGCCTGT460.15269709543568466No Hit
GTAATAGGGATGTGCTGTTTGGTTTTCTGTTCCTGTGTGTGTTTGCTGAGAA450.14937759336099585No Hit
TGTTGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA450.14937759336099585No Hit
GCTGCAGGGCGATCTTACGTTGGGACTCAGGGGCTTCCAGGAAAATGCCAGC450.14937759336099585No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTACATCAATCTCGT450.14937759336099585No Hit
TACTAGGGGATTCTTCACATCATTCAGTAGACCTATTCTAGCAAGTTACTTC450.14937759336099585No Hit
ACATGCGGGAAGTACGAGAGTGGCCCTGACGGAGGGGAGGAAATGAGGAAAA440.14605809128630703No Hit
TATCGCGGGACGACGCCTGGCTGATTTTTTGTATTTTTGGTACAGACGGGAT440.14605809128630703No Hit
AATGTGGTGGGTATAAGCCTGGATGTTGATGGTGGGTGAAGGTGGTGGGGTC440.14605809128630703No Hit
GGGCGGGGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAA430.14273858921161825No Hit
GCTGACGGGCTATTACTATACTGGAGAAGTCAATGGTACCTACTATCAATTT430.14273858921161825No Hit
CCTACTGGGGAATAGAACGTGAAAGCCAGCTCTTTCAGAATATCCTCTATTA430.14273858921161825No Hit
GAGTACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC420.13941908713692946No Hit
AGTTTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC420.13941908713692946No Hit
TTCATTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC420.13941908713692946No Hit
ATGCTTGGGATACATAAATAATGTAAAATAATTTGGGATTATTTCAACTTTA420.13941908713692946No Hit
AACTTAGGGCAGGATGTAGGAGATGACAGTGCTTTATGAGCCTTAAATTCGG420.13941908713692946No Hit
TGGCCTGGGCATAATAAAGGAACTTTCAGAGATTGGTTGAAACCCAGTGGTA420.13941908713692946No Hit
AGGCATGGGCAGACATTCCATATAAATGGAATAACTGATTTTTTTACACAAA410.13609958506224065No Hit
TGAATAGGGGATCTTCGGCTGGGAAGTAGAATCAATGAAGGAGGAGATAGAG410.13609958506224065No Hit
TAACGCGGGAGGTCGTCCTCTCTGTATGTGCCCCGCGCATTTCCTTGGCCTC410.13609958506224065No Hit
TGTAAAGGGTGCCGGGGATGCTAGGCACACTGCCCCCTTGAATAGACATTAT400.13278008298755187No Hit
TAAGCTGGGAATATTTAGTGGGTTTGTTTTGGTTGGTTGGTTGGTTGGTTGG400.13278008298755187No Hit
AGAGAAGGGGATAGAACTTTCTGGCAAAGGTGTATACATTTGTCACAGCTCA400.13278008298755187No Hit
TACCTAGGGACAGTCTAAAAGTCTTGATGGTAACTATAGTGTAATTATCTTT400.13278008298755187No Hit
CGACTTGGGATTTCAGTTTCCTCTAGGGCTCAGGATACAGTGTTTTTTTTTT390.12946058091286308No Hit
GCAACTGGGTGCTGCTAAAAATGACAACTCCCTCTGTGTCCTGTTTTTCTTA390.12946058091286308No Hit
AATCTAGGGAAGAGACTTAAGTTGTATAACCACAAGGTCACATAACTAGATC390.12946058091286308No Hit
CTTTTTGGGATATGTTCTCAGTATTTTGGGAGCCTTTCCACTTTTTTAAACC380.12614107883817427No Hit
TGATGAGGGCTCTAATAAATTTAACACAGAGAATAGTTTCCCCATTCACTTT380.12614107883817427No Hit
CCGTAGGGGAAACTTCATCCAATGGTTTCCTAAATGGTAGCTCTAAACATGA380.12614107883817427No Hit
GACACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA380.12614107883817427No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTACATCAAACTCGTATGC380.12614107883817427No Hit
AGCTCAGGGCCACACCAGAGCTAGAGTTTTCTTTCTACAATCCAACTGATTC380.12614107883817427No Hit
GATCGAGGGATTTGAATGGATTGTTGCTTTTCACTAGCTTGTATGGTATGTC380.12614107883817427No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTACATCAAT380.12614107883817427No Hit
TCACGAAGGGGATGCTGTGTGCCTGTCAAGATTCCCTCCGGTTTTCTGGCTA380.12614107883817427No Hit
GTCAAGGGGGATTTGCAAAATGCAGATTATATTTGATAGGCTATAGTATGTA380.12614107883817427No Hit
CCCCTCCCAAGCCCCCCTTGACATCCTCTCAGCTACTCCAGGGCACCTGACT370.12282157676348547No Hit
AGCTAGGGGGTGGGGCAGGTAGCTATCCCTCCCATGTCATTAGTAATCCTTT370.12282157676348547No Hit
TGCTTAGGGACCCCTGAGACTGAATTTGCCCTGCTAATGAACTGAGGCATAT370.12282157676348547No Hit
CACATTGGGATTGGAGGAGAGTTTAAAAAATACTTAGAGGTTGTATTGAATA370.12282157676348547No Hit
CTTTCTGGGCAGTGGCTAACCTGGTTAGAAACTGCTGAAGAAGAAGAATCAG370.12282157676348547No Hit
GAAACTGGGCAAGCCCAAGAAGAAAGTCTTCGAGAAGTTGCAGGCTGACTTC370.12282157676348547No Hit
TAGATTGGGATGATCAATGGGCTTTGGACCATGTGGAGGTCGTCCTAGTAAG370.12282157676348547No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTACATCAATCTC360.11950207468879669No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTACATCAA360.11950207468879669No Hit
GTAGCTGGGAATAATAAAAACAATAGTATGGAAATAATAAAAACTTAAAAGG360.11950207468879669No Hit
TGCAGTGGGACCAAAATCAATTCTTGATTGTACACAGACAGCATTGGTTCTA360.11950207468879669No Hit
ATAGCAGAGGGATCGCCTTGTGGTACACCCGGGACCGCCTCGTGCCAGGGCC360.11950207468879669No Hit
GACCAGGGGATCTACCATCTGATTGGCTGGTACCGAGAGCTGTGGGTAAAAT360.11950207468879669No Hit
TTACGCTGGGCCAGGAAAAGCCTTGCTTGCCTGTTTCCCAAAGAGCTCTAAG350.11618257261410789No Hit
CCTTATGGGGGAGACTGCTGAGTCACTGTGGCCATTTTTAGATAAACCTTTT350.11618257261410789No Hit
CAGCAAGGGCAGGCCATCAGCATCCGGGACCATATGAATGCCAGTGCCCAGG350.11618257261410789No Hit
ACAAATGGGTCTCAAAGCGGGCATGAGTCATACAACCAGTAAGGCTTACCAA350.11618257261410789No Hit
CAAACAGGGATGCTAATTATTTGTATAATACTTAGTTGGCTTTATCTGTGGT350.11618257261410789No Hit
GCTCAGGGGAGATTGCTTAAGCCCAGGAGTTCAAGTTTACGGTGAGGTGTGA350.11618257261410789No Hit
GGTCTAGGGTGAAAAGTTCCAAAATATAGTTTATTGTATCTTTCATCACTAA340.11286307053941907No Hit
CTTCATGGGAAACTTATTCCATTTATAATGTTCTCTTTATATTGGTTATGCT340.11286307053941907No Hit
CTATATGGGGGTTTTTAATTTTGTGTTGCTAAAAAAAGTGCATGATGGTGAG340.11286307053941907No Hit
GAGCAGGTGGCATCTATTTATGCAACTAAATACTTTTCAGAAATGGTATATA340.11286307053941907No Hit
TCTACAGGGCAGCGAGGTGGTGCTGACAGTGTGGACTTGAGCACACTTATGC340.11286307053941907No Hit
TACAAAGGGAATAACCAAAGACCTGAGCATGTCACTATGGGGGTCCTTGGCA340.11286307053941907No Hit
ACTGTAGGGACTATTTATTATAAACTCTATATTACAAATAAAGAATTGAGCC340.11286307053941907No Hit
GGCTCGGGGCCCAAGGAGGGGGCCTCATTGTGTCTCCCGTGCATGCTCTGCA340.11286307053941907No Hit
AACGCAGGGAGAGCAAAGGGATGGGTCCTTGAGGCCTGCCAGTGTGTAAAGG340.11286307053941907No Hit
GCATCAGGGTGTGTCTCATCATTGCACTGTTCTTTTAAAATTGTATATATAT340.11286307053941907No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCTACATCAATCTCGTATGCC340.11286307053941907No Hit
CACGACGGGTTATTCAGCCTTAAACGAGGGAAATATTCTGACATATGTTGCA340.11286307053941907No Hit
TCACATGGCAAACAACTAGTAAGAAGCAAAACCATCATAAATAAGAGTTCTC340.11286307053941907No Hit
TTCCGGGCACCCTTTTGTATCCACCCTTTCCAGCTACATTACTTTAGACAGT340.11286307053941907No Hit
AGCCTGGGACAACCTCAGAAGAAGTGTTTCGAGTTTAACATGCGCTGTTTCT330.10954356846473029No Hit
CGCTTTGGGTCCTTACAGCCCTGCTGCTGGGGTTGATTTTGAACTGGAGTCA330.10954356846473029No Hit
TAGATGGGGCAGGGCTTCTATTTTTCAACATGTGACATCAGAATAAAGTTCT330.10954356846473029No Hit
AGCTGAGGGTGTGTACTGAATAATGTAGGCAGTTGTAATGTAACACCTAGTG330.10954356846473029No Hit
GTACGGGGGGTACGTGAATGTTCACAGCAGCATTATTCACAATGATTAAAAG330.10954356846473029No Hit
TGACGAGGGTGCATACAAATTTGGAAAAACTGTGGTGAGCTGTGAAGGCTAT330.10954356846473029No Hit
GATTAGGGGACTTCGGATCTGCTTGTGAAGAAAAGGTGGACCCCTGCGCCTC330.10954356846473029No Hit
TATTAGGGGGCTTTGGAGGTCAGTCATTGAAGTCCCTGCTGTGCCAGAGGAA330.10954356846473029No Hit
CGTCAAGGGGCCTCGGGGAGAGTGTAGTCAACAGTAAGTTCTTTTAATGAAT320.1062240663900415No Hit
TGAGTTGGGAGAACCCATGTCTCTAAACTGCTAGATCAGCCTGCGTTATTTG320.1062240663900415No Hit
TCCTGAGGGAGATGTTGAATAAATGAATCACCTCTTTATAGTGCCTGTGCCC320.1062240663900415No Hit
TTATGAGGGATACTACAGATGGTAAGTATTTAAGCCTGTAAGTAAGTAATTA320.1062240663900415No Hit
GTTCGCGGCTGATGTTTTTATTGAACATTGTGTTCATGCTTTTGAAGAATTA320.1062240663900415No Hit
GTTTGGGGGATCCAGAAGGCGTATCCGTTCTATCAACAAACTCATCCGAATT320.1062240663900415No Hit
ACATATGGGCAATTTATCTAGATTACCTTACCTTGGTAGTAAATTTTGAAAT320.1062240663900415No Hit
GCTATGGGGTGGTTTTCTCCAGGGATTTAAGGGCCAGCTGATACACACATAC320.1062240663900415No Hit
AAGCTCGGGACTTTTCTGGGTATGTATTACTATCATCAGTCATGCACAGCTA320.1062240663900415No Hit
GCTGCTGGGCAATATAGCAAACCTGCACATGTTCCCCTGAACCTAAAATAAA320.1062240663900415No Hit
TTATTAGGGATTTTTGGTGAAAAAAGGGGATGGAAATTCTTATAATCTCTTT320.1062240663900415No Hit
GAGCGAAGGGCAAGCTTTGCTGTGAATAAAAGGCTTACATTTGTTCCTGCAT320.1062240663900415No Hit
CTACAAGGGTAACACAAAGATACATAGAGATGAGAAATATTAAAGAGATATG320.1062240663900415No Hit
ACTAGCGGGGTGTCCCAGCTTTGTCACTTCTAGAACAAGACCATGAGTACAA320.1062240663900415No Hit
CAGAACGGGAGAAGAGTAGAGACCTAATTTAGCATTTTCCGGCAATTTGACA310.10290456431535269No Hit
ACTCGCGGGATGCATCTCTTGGCTGTACTATAAGAACACATTAATTCAATGG310.10290456431535269No Hit
GTCTGCGGGACATATATATATATTCCTAGACATCTAGTGTTTGCTGTCATTA310.10290456431535269No Hit
ATTCATGGGTCATTTGTCAAAATGCTTCTTTTGTTGCCACAGTAAAGAACAG310.10290456431535269No Hit
CTATATGGGACGCTGCAACAATCACTTTTATTTCTTACACTGCTCGTGGGCT310.10290456431535269No Hit
TGCTGTCGGGGTGTTTTAGATCACCTGAGAACATTTCTTGAACACTAGTATT310.10290456431535269No Hit
GCGTACGGGGCCTTTGAGGGCAAGACATTTTAGAGCTGGGCAAAACCTGTGT310.10290456431535269No Hit
GGGCTGGGGGTGTAACTTTTCAATTTTGCTCATTTGAATTGGGTCTGTCATA310.10290456431535269No Hit
AAACAAGGGATTGCTCAGGATCGCAATATTGCCATTCAGAGCCAATTTACAA310.10290456431535269No Hit
GTTCAGGGCCAGCTTTTAAATACCCTGTATAAGAAAGTTATCTTTTTATACA310.10290456431535269No Hit
ATGCACGGGAGATCCTGTGATGGCGAGACAAATGATCCTTAAAGAAGGTGTG310.10290456431535269No Hit
GTTGCGGGAAGTGGGTAACATTTTATGCAGTTTAAATGAATGGAATATTTTC310.10290456431535269No Hit
GTGTATGGGAGGCTGCCTAAGACATGTAACGAAACACTTCAATAATTGTCCA310.10290456431535269No Hit
CAATTAGGGCATTTTCCTGCCTGGTTGCTGAGACTCCATTTTGCTGCCACAC310.10290456431535269No Hit
GTACTGGGGTACCTGAGGCTGTTGGTCAAGTTATACAGGTTTCTGGCCAGAA310.10290456431535269No Hit
GCCTGGGGACTTGTTTTTTACATTTTAAGAGTTGTAGAAGTTGTTCCTAACT310.10290456431535269No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)