FastQCFastQC Report
Sat 31 Dec 2022
SRR5846480.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846480.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30795
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTACATCAATCTCGTATGCCGTCT5191.6853385289819773No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTACATCAATCTCGTATGC4361.4158142555609676No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTACATCA960.3117389186556259No Hit
GCTCGGGGGGTGCGGCGCCGTCGATGGTGACGAATTGCGCATAGTCGACGCC940.3052443578503004No Hit
GGTTCAGGGCTGAAGAAGAAAGGTGGCAAATACAGTGTTCAGTTCAAGTTGC710.23055690858905667No Hit
GCAGTTGGGCAACCCGGGACAATCAAAGGTATATAGCTACATACGCATGTAT690.2240623477837311No Hit
ACACTGGGGTCCAGAATTCATGAGGAACTTAAAAAACTCAACAACAAAAATC660.2143205065757428No Hit
CGCAAAGGGCTGTTAGCGGGGATCTATCCCTGGGCTGAAATAAAAGAGGAAA640.20782594577041727No Hit
CTTATACACATCTCCGAGCCCACGAGACCTACATCAATCCTGTCTCTTATAC630.20457866536775451No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTACATCAAACTCGTATGC630.20457866536775451No Hit
GAAGCAGGGATCATTGTGTAATTTGTAAAGATTCACGTGTAGTTCTTTATTA620.20133138496509173No Hit
GCAGCTGAACCCCACGCAGGCCTTCTTCCTGCTGGTGAACCAGCACAGCATG600.1948368241597662No Hit
GAAGATGGGACGAGAGCCAGCAAGCAAAATAGATGTGGCTGGGTCTGCCTGT590.19158954375710344No Hit
CAAGCCGGGTCAGCAGGAAGTATTGTCTGTGTGTGATGACGGGGCAGTATTG580.18834226335444065No Hit
TGTTGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA580.18834226335444065No Hit
CTATATGGGGGTTTTTAATTTTGTGTTGCTAAAAAAAGTGCATGATGGTGAG570.1850949829517779No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTACATCAA570.1850949829517779No Hit
AGGAGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC560.18184770254911511No Hit
CGAGTCGGGCTACCTAATTACCTCCCAAAAGACCCTACCTTCTAATACCATC550.17860042214645236No Hit
CTAGTTGTGGGAGGAAAGGGTTAACTCAGCAGGTCTTGTTTGCCCAGACTCT530.17210586134112682No Hit
ATACCGGGGTCAATGGATTTGCAATAACAATCCAAAGATGGAAGAAGACACT530.17210586134112682No Hit
TATGCTGGGACCATCTTTGCCCCCAGGATACAATTCTTTGCCATTGAAATTG530.17210586134112682No Hit
AGGACCGGGGGTTGACGCGGCCTGGGCAGATCCTTGCAGAAAGCTGCAGGCT530.17210586134112682No Hit
AGAAATGTGGAGATCAGATTAGAAAAAAGAAAATCAAAACAAAAAAGACAAT520.16885858093846404No Hit
GGCTCGGGGCCCAAGGAGGGGGCCTCATTGTGTCTCCCGTGCATGCTCTGCA520.16885858093846404No Hit
GTAATAGGGATGTGCTGTTTGGTTTTCTGTTCCTGTGTGTGTTTGCTGAGAA500.1623640201331385No Hit
GCTTTAGGGACGTTGGTATAATCACATCAAGTCTTACGAAAAGGAAAAGGCC500.1623640201331385No Hit
TCGCAGGGGGATCACATTTCAGAGATGCTAAAATGTGATGTCACAGGTGTCT500.1623640201331385No Hit
AGAACTGGGAGTGGGAAACTTTAACATCCAACTGTCAAAATTAGACAGATCA490.15911673973047574No Hit
CCCCTCCCAAGCCCCCCTTGACATCCTCTCAGCTACTCCAGGGCACCTGACT490.15911673973047574No Hit
AGCTAGGGGGTGGGGCAGGTAGCTATCCCTCCCATGTCATTAGTAATCCTTT480.15586945932781296No Hit
GCTTAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA480.15586945932781296No Hit
AGGCATGGGCAGACATTCCATATAAATGGAATAACTGATTTTTTTACACAAA480.15586945932781296No Hit
AGTTTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC480.15586945932781296No Hit
CGCGCTGGGCATTAACCTTTCCACCTTAAGACACTAGAAAGAGAACAAACTA480.15586945932781296No Hit
CGTCAAGGGGCCTCGGGGAGAGTGTAGTCAACAGTAAGTTCTTTTAATGAAT470.1526221789251502No Hit
CGTCAGGGGGAAGCTTCTTCATGGAGTAAAAAATGTATTTAAAAGAAAATTG470.1526221789251502No Hit
TGCACTGGGATATATTTCGGGGTAGTGTGAGCTTTTAATGTTTAAGTCATAT470.1526221789251502No Hit
GCAAATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA470.1526221789251502No Hit
TAACGCGGGAGGTCGTCCTCTCTGTATGTGCCCCGCGCATTTCCTTGGCCTC470.1526221789251502No Hit
AATCTTGGGACATCCAGATTATCTGGAATAAGCTCCCTCACTTAAGGTCACT460.14937489852248742No Hit
CTATATGGGACGCTGCAACAATCACTTTTATTTCTTACACTGCTCGTGGGCT460.14937489852248742No Hit
CTACAAGGGTAACACAAAGATACATAGAGATGAGAAATATTAAAGAGATATG460.14937489852248742No Hit
CCAATAGGGATGTTTCCAAATCTGTGTATTGTGATTTTTACATACTAATGAA450.14612761811982464No Hit
AGAGAAGGGGATAGAACTTTCTGGCAAAGGTGTATACATTTGTCACAGCTCA450.14612761811982464No Hit
AAACAGGGGATTTTTGTAAGCCTAAAAATGATTCTTTGAAAGTTTAAAGAAA450.14612761811982464No Hit
ACTAGCGGGGTGTCCCAGCTTTGTCACTTCTAGAACAAGACCATGAGTACAA450.14612761811982464No Hit
ATATCCGGGGCCATTCTTAAAAATAAAAAAGCTCCCACCCAGAAACTCTAGT440.14288033771716188No Hit
CATTTAGGGACTACAATGAGGCTGCTGGTAACAAATATGTACCTCGGGCCAT440.14288033771716188No Hit
ATTCCAGGGATAAGAAAGGACCTATAGTTATATTTATTTTGCATTTGTTCTT440.14288033771716188No Hit
GAGCGAAGGGCAAGCTTTGCTGTGAATAAAAGGCTTACATTTGTTCCTGCAT440.14288033771716188No Hit
ATGCGTGGGGCTGGGGATGGTGGGAGCCTGCGCAGCTGGCTGGGCGGTCTCT440.14288033771716188No Hit
TATCGCGGGACGACGCCTGGCTGATTTTTTGTATTTTTGGTACAGACGGGAT430.1396330573144991No Hit
AACGCAGGGAGAGCAAAGGGATGGGTCCTTGAGGCCTGCCAGTGTGTAAAGG430.1396330573144991No Hit
GACCTTGGGACGAAACATCTGTGCAGTTTTCAGAGTGTCACAAAGTCAATAG430.1396330573144991No Hit
GTACGGGGGGTACGTGAATGTTCACAGCAGCATTATTCACAATGATTAAAAG430.1396330573144991No Hit
TTTCGCGGGGGAAGACCAGGCGGGCACTGTTGCCCGGCGACGCTCCGGCGCT420.13638577691183634No Hit
GGGCTGGGGGTGTAACTTTTCAATTTTGCTCATTTGAATTGGGTCTGTCATA420.13638577691183634No Hit
CCTACTGGGGAATAGAACGTGAAAGCCAGCTCTTTCAGAATATCCTCTATTA420.13638577691183634No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTACATCAATCTCGT420.13638577691183634No Hit
GGTCTAGGGTGAAAAGTTCCAAAATATAGTTTATTGTATCTTTCATCACTAA410.13313849650917356No Hit
ATAAATGGGATTCAAAAGGGTATCTCCAGAGAGAAAGTCCAGTGGGGAGTGT410.13313849650917356No Hit
CGACTTGGGATTTCAGTTTCCTCTAGGGCTCAGGATACAGTGTTTTTTTTTT410.13313849650917356No Hit
TTCATTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC410.13313849650917356No Hit
ATTAGAGGAGTGAGTCATTTAAATACTCTACGTTTGGTTCAATTAACCAGTA410.13313849650917356No Hit
GATCGAGGGATTTGAATGGATTGTTGCTTTTCACTAGCTTGTATGGTATGTC410.13313849650917356No Hit
CAGCAAGGGCAGGCCATCAGCATCCGGGACCATATGAATGCCAGTGCCCAGG410.13313849650917356No Hit
GCTCACGGGATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAG400.1298912161065108No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTACATCAATCCTGTCTCT400.1298912161065108No Hit
TGCTGTCGGGGTGTTTTAGATCACCTGAGAACATTTCTTGAACACTAGTATT400.1298912161065108No Hit
AGGCATGGGGGAATTAACTTGCAAGTTCTAAGTTCATATATAAAATTCAATT400.1298912161065108No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTACATCAATCTCGT390.12664393570384802No Hit
ATTTTGGGGCAAATGTCTGGAATGTGTTACTCTACTACATGTTTTTTGAAAT390.12664393570384802No Hit
GTAGCTGGGAATAATAAAAACAATAGTATGGAAATAATAAAAACTTAAAAGG390.12664393570384802No Hit
TGCACAGGGGCGACAACTGCAAGACTCCATCTCAAAAAAATAAAAATAAAAG380.12339665530118527No Hit
ACGTAAGGGAATCATGTGGCTGTGAAATTCACAGTAATATGGTTCCCGATGA380.12339665530118527No Hit
GGCTGTGGGCTGGTGGAAGGTGCCTTCTTGAACTTATGTTTCATTTAGTCAC380.12339665530118527No Hit
TTACGTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA370.12014937489852248No Hit
TTTGAAGGGGAGGCATTGAGGCAGCCAGCGCAAGGGCTTCTGCTGAGGGGGC370.12014937489852248No Hit
CGCATGGGGGCCATAAAGAAGGTGAGAAGAGCAAAAATACTGCTGCTATTAA370.12014937489852248No Hit
TAAGCTGGGAATATTTAGTGGGTTTGTTTTGGTTGGTTGGTTGGTTGGTTGG370.12014937489852248No Hit
TAGCCCGGGCTCCTTTTCCTCCTTCTCCTCAACCTTTTCAATGTGAAGACGA370.12014937489852248No Hit
AATGACGGGCAGTTCATGTAGAAAAAGTGAACTATCTCAAAAACTTCTGTGC370.12014937489852248No Hit
AATGCTGGCTAGACGAGGGGAAGTAGGAGGATGATATCTACCATCATGAAAC370.12014937489852248No Hit
GTGATAGGGAACAGTCGACACAGAGGTCTTTGAGTTGGATGGTTGCAAATAT370.12014937489852248No Hit
AGTAATGGGCCTTTCTCTCTCTTTCTGTCAGTAATCTGGATAACGATTTATT370.12014937489852248No Hit
TGATGAGGGCTCTAATAAATTTAACACAGAGAATAGTTTCCCCATTCACTTT360.11690209449585973No Hit
AAGGCTGGGATCTTATAGAGTTATTGATGATGGTTAAATGAGATAATCGGAG360.11690209449585973No Hit
CTGTATGGGGCCATAAAGAAGGTGAGAAGAGCAAAAATACTGCTGCTATTAA360.11690209449585973No Hit
ACAGCAGGGGAATGCCATTTTTTGTGTTTTTAAATTATTTTCTTTATCTTGT360.11690209449585973No Hit
TACCTAGGGACAGTCTAAAAGTCTTGATGGTAACTATAGTGTAATTATCTTT360.11690209449585973No Hit
CCCCCAGCCCCATGATCGAAAGCACCCCCATGTCCATTGCTGGCGATGCCAC360.11690209449585973No Hit
TTATTAGGGATTTTTGGTGAAAAAAGGGGATGGAAATTCTTATAATCTCTTT360.11690209449585973No Hit
AATGTGGTGGGTATAAGCCTGGATGTTGATGGTGGGTGAAGGTGGTGGGGTC360.11690209449585973No Hit
GTACTGGGGTACCTGAGGCTGTTGGTCAAGTTATACAGGTTTCTGGCCAGAA360.11690209449585973No Hit
TTATGAGGGATACTACAGATGGTAAGTATTTAAGCCTGTAAGTAAGTAATTA350.11365481409319694No Hit
ACTCGCGGGATGCATCTCTTGGCTGTACTATAAGAACACATTAATTCAATGG350.11365481409319694No Hit
TATTGCGGGTTATGGATACTAGGCTTGATATATGGGTGATGACATGATCTGT350.11365481409319694No Hit
GATAGTGGGCACAGTGAATAATCTGTTAGTATTCTTCAAGACCTCTTATCTG350.11365481409319694No Hit
TTAACTGGGAAGCACAGCAGATGTGGAAAGGAAGGAATAAAAATGCCCAAGA350.11365481409319694No Hit
TGAATAGGGGATCTTCGGCTGGGAAGTAGAATCAATGAAGGAGGAGATAGAG350.11365481409319694No Hit
GCATCAGGGTGTGTCTCATCATTGCACTGTTCTTTTAAAATTGTATATATAT350.11365481409319694No Hit
ATGCACGGGAGATCCTGTGATGGCGAGACAAATGATCCTTAAAGAAGGTGTG350.11365481409319694No Hit
CAATACGGGGTTTCACTGGTATCTATTGCATTGTATAATAAATGACCAGATG340.11040753369053419No Hit
CTTCATGGGAAACTTATTCCATTTATAATGTTCTCTTTATATTGGTTATGCT340.11040753369053419No Hit
GGGCGGGGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAA340.11040753369053419No Hit
GCTCATGGGGTGTCATGATTTCAATGATTGTTTAATTGTAGGTCAATGAAAT340.11040753369053419No Hit
TTACGCTGGGCCAGGAAAAGCCTTGCTTGCCTGTTTCCCAAAGAGCTCTAAG340.11040753369053419No Hit
GTCTGCGGGACATATATATATATTCCTAGACATCTAGTGTTTGCTGTCATTA340.11040753369053419No Hit
CATGTTGGGTCTGGCCACTGCACTGTAGATAATTCATTGGAAACAAGATTTA340.11040753369053419No Hit
CTTTCTGGGCAGTGGCTAACCTGGTTAGAAACTGCTGAAGAAGAAGAATCAG340.11040753369053419No Hit
CAATTAGGGTTATGGTTTCCTCTCATTTCTCTATGATATAATGACTCCTTTT340.11040753369053419No Hit
TGGCCTGGGTTCTGTAGCCTAGGAACTGCCTCAGTGTCTTTGCCAGAAAAAG340.11040753369053419No Hit
GCTGCAGGGCGATCTTACGTTGGGACTCAGGGGCTTCCAGGAAAATGCCAGC340.11040753369053419No Hit
AACAGGGGGAAAGAGTTTCAGGGGCTCATAATTTTGAGAGATGGAGGAAGTT340.11040753369053419No Hit
GTCAAGGGGGATTTGCAAAATGCAGATTATATTTGATAGGCTATAGTATGTA340.11040753369053419No Hit
TAGATTGGGATGATCAATGGGCTTTGGACCATGTGGAGGTCGTCCTAGTAAG340.11040753369053419No Hit
TTCCGGGCACCCTTTTGTATCCACCCTTTCCAGCTACATTACTTTAGACAGT340.11040753369053419No Hit
TCGACTGGGCAGACTCCATTGGGCACATCTGGGAGGAAGGCAACCTCCTTTG330.1071602532878714No Hit
GTCACAGGGGTTTGAGATCGGCCTGGACAACGTGGCAGAATCCCATCTCTAC330.1071602532878714No Hit
TCTCAGGGGCTTGTGCTTTTACTTTGTAAACATTGTACAAATGTATTTGGAA330.1071602532878714No Hit
GGCTCAGGGAATCAGCAGTTAAGAAGGTTCTAGAACCCATGAAAGCTTTTGT330.1071602532878714No Hit
CGTAATGGGAATAAATAAAAAGAAAGAAAGAAATGATGTAGTGAAAATATAG330.1071602532878714No Hit
CCGTAGGGGAAACTTCATCCAATGGTTTCCTAAATGGTAGCTCTAAACATGA330.1071602532878714No Hit
GGTTCTGGGACCTCTAAGAGAACTTTCTGAATTTGCAGCTAATCTCGGGGAA330.1071602532878714No Hit
CATAATGGGACAGTGGTTACATAATACACTGGCTCACTGCAGACTCTCTTGT330.1071602532878714No Hit
GTTCAGGGCCAGCTTTTAAATACCCTGTATAAGAAAGTTATCTTTTTATACA330.1071602532878714No Hit
ACAGCAGGGGGGAATGGGAGTGCCTGGACACTACCCAGAGGAAATTGTACAG330.1071602532878714No Hit
AATCTAGGGAAGAGACTTAAGTTGTATAACCACAAGGTCACATAACTAGATC330.1071602532878714No Hit
CGTTGCGGGATCATGCTTAAAGATCATTCTCAGGACTTTACAAAAAGTCCAA330.1071602532878714No Hit
AACTTAGGGCAGGATGTAGGAGATGACAGTGCTTTATGAGCCTTAAATTCGG330.1071602532878714No Hit
TTGCCAGGGAGATAACTGATTATAGGAGTTGAAGTTCAGGAGTGAAGTCTGA330.1071602532878714No Hit
CTTCTAGGGATGCCCAAGGCCTAGGTTATGGGTACGGCAACCACATGTCCCA330.1071602532878714No Hit
GTTTTAGGGGATTTGGAAATAGGATGTTTTCAACGTTCTTTTGTCTTTTGCT330.1071602532878714No Hit
TGCGAGTGGGCAGGAAATAGGCCCAACCACCAGATGAGAATTAGGCCCTGGA320.10391297288520863No Hit
TGTAAAGGGTGCCGGGGATGCTAGGCACACTGCCCCCTTGAATAGACATTAT320.10391297288520863No Hit
GTAGAGGGGACAATGGAGTGGGGAAGTACTTTCATTACCCAAGCCTCAGAAA320.10391297288520863No Hit
GTATAAGGGCAATGTCAGTACTGAAACTCCTACTCTCCCCTCCCGCCCCACT320.10391297288520863No Hit
CCACAGGGGCGCTCTTCCGATCTCATCCTGGGATAATGTCTGCGTCACCATT320.10391297288520863No Hit
CAAGAGGGGCTTACGATTTTTCTCTGTCTTATGAAACAAGAAAGATCTAGCC320.10391297288520863No Hit
GCTGACGGGCTATTACTATACTGGAGAAGTCAATGGTACCTACTATCAATTT320.10391297288520863No Hit
GAGCAGGTGGCATCTATTTATGCAACTAAATACTTTTCAGAAATGGTATATA320.10391297288520863No Hit
AGCTCAGGGCCACACCAGAGCTAGAGTTTTCTTTCTACAATCCAACTGATTC320.10391297288520863No Hit
AGCAGCGGGTAACTGTATACTGGATGTACATTTTCCCATGGTTGTCTACAGG320.10391297288520863No Hit
TCACATGGCAAACAACTAGTAAGAAGCAAAACCATCATAAATAAGAGTTCTC320.10391297288520863No Hit
TACTAGGGGATTCTTCACATCATTCAGTAGACCTATTCTAGCAAGTTACTTC320.10391297288520863No Hit
GCAGCTGGGAATTTTGATTTGTCCCAAGATTTTCAGCCTATTTCTGATCTAG320.10391297288520863No Hit
TCTAATGGGAACGTAAGGTTAGTAAAGCATACAAAGAATCTAGTGTGCTCAG310.10066569248254587No Hit
CTGGTCGGGACTTCTTCCCTGGGCGGTGTGGGTTTCCCCCAGCTCTTCCCAC310.10066569248254587No Hit
CGCTTTGGGTCCTTACAGCCCTGCTGCTGGGGTTGATTTTGAACTGGAGTCA310.10066569248254587No Hit
GTAACTGGGTGCTCTGGATTAAATGATGAAACTTATGGCTACAAAGAGGGCA310.10066569248254587No Hit
GTTCGCGGCTGATGTTTTTATTGAACATTGTGTTCATGCTTTTGAAGAATTA310.10066569248254587No Hit
GGGCTTGGGCTTGGAAAGAAGGAATAAAGAAAACAATTGAATGGTACAGAGA310.10066569248254587No Hit
ACACAAGGGGATCTGATCAGGGGATGTTTTGCATAGAGACAACCACAAAGCA310.10066569248254587No Hit
GTATATGGGCAGAAGTTGATGAACATACACAAAAGACGGGTGTCTTAGCTGC310.10066569248254587No Hit
TAACTAGGGACTGAGGCAAGAGCACTCAGGGTGAACATAGTCAAGTTACTTT310.10066569248254587No Hit
TTAAAAGGGCAACTGTTCTGATAATGAATTCACCCAAGCTTTAACCGCAGCT310.10066569248254587No Hit
TCTACAGGGCAGCGAGGTGGTGCTGACAGTGTGGACTTGAGCACACTTATGC310.10066569248254587No Hit
ATGCTTGGGATACATAAATAATGTAAAATAATTTGGGATTATTTCAACTTTA310.10066569248254587No Hit
TTCCTTGCGGGATGAGAAAACATCTATTTTTATCACTATTTTATAGCTGTCA310.10066569248254587No Hit
AAACAAGGGATTGCTCAGGATCGCAATATTGCCATTCAGAGCCAATTTACAA310.10066569248254587No Hit
TCCGATAGGGGGGCGGCAGAAGCGGCGCTCGCGCCAAGGGACGTGTTTCTGC310.10066569248254587No Hit
CACCCTGGGCTGCAGAGGTATTCAGTTCCTGGCACAGGGGACTAGGGGCATG310.10066569248254587No Hit
TATTCTGGGCTGGATGATTCATCCCAAAGCCTTCCCACCCAGGTGTTCTCTG310.10066569248254587No Hit
ACAAATGGGTCTCAAAGCGGGCATGAGTCATACAACCAGTAAGGCTTACCAA310.10066569248254587No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)