FastQCFastQC Report
Sat 31 Dec 2022
SRR5846479.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846479.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84907
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTACATCAATCTCGTATGCCGTCT10121.1918923056991766No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTACATCAATCTCGTATGC8460.9963842792702604No Hit
CTTATACACATCTCCGAGCCCACGAGACCTACATCAATCCTGTCTCTTATAC1460.17195284252181797No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTACATCA1320.15546421378684913No Hit
TACGAGGGAAACCAGACTATTTTAGAAACAAAAATAATGTTGAATTCATTAA1230.1448643810286549No Hit
GCAAATGGGAGGAAGAAGAAAAGAAAGGAGGAGGAGAAGAGGAAAGAAGGAG1070.1260202339029762No Hit
TGTATAGGGTTTCAAAAGAATTATGAACACACCTTTCCTGGATCACAGATTA1060.12484247470762129No Hit
AGCCTAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA940.11070936436336228No Hit
TCTCAAGGGCTTTTGGACAGTAATTTCTATCACAGTTAGAAGGAAATGATAG930.10953160516800735No Hit
ACGTGCGGGGCATTTTATGGTACTTCTTTCAAATTAACTGTCCAAGCCAGGA930.10953160516800735No Hit
GCGTGGTGCTTCTTTAGTAAATACTGTACAGATTTTACCATGGAGAACTTTT920.10835384597265244No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTACATCAATCCTGTCTCT910.10717608677729752No Hit
CGACCTGGGACCCACCACTGAGTAGAAACAGGAGGATGTGCTCCCAACATAC900.1059983275819426No Hit
CAGCTGGGATCGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT900.1059983275819426No Hit
CATAAAAGGGCATGTGGTAGTGACTGGTGTCCCCTCTGTAATCTACCCTAGC880.10364280919123277No Hit
ACTCTAGGGAGTCTTCTGTTCGAGTGAGCCTGGGAGTAAACACTCCTGCTCG880.10364280919123277No Hit
GTTCGCGGGTTTGAACAAATGGTAACCCATTTTTCCTGCTCTGCTGTTTCCT880.10364280919123277No Hit
CAGAAAGGGCAGCTCATGTACGTCTGTTTTTATAAGATCAATATTAAAACCC870.10246504999587784No Hit
CCAGCCTGGGTGACAGCAAGACCCTGTCTCTAAAAAATTAAAAATTAAGAAA870.10246504999587784No Hit
TCACCAGGGGATTCACTGCTGGAAGTACCTGAGTGTGCAGGGCCAGCTCTTC860.10128729080052293No Hit
CCACCCGGGTGCTGTTCTCAGCTCCAGCTTCAAACCTCGAGTCTCGAGAGGG860.10128729080052293No Hit
GAACATGGGATAATTAAAAAGAACCAAGCAGAAATTCTAGAGTTGAAAAATG860.10128729080052293No Hit
TATGGCGGGGATAGCTTGCTTTATTTCTGCCTTTACGTTGACAGTGTTGAAG850.100109531605168No Hit
GAAAGAGGGTCTTATAATTTAGTAAATAAATTCTTTGTTGGGAAAACTTTAG850.100109531605168No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)