FastQCFastQC Report
Sat 31 Dec 2022
SRR5846477.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846477.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82381
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTACATCAATCTCGTATGCCGTCT9771.1859530717034268No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTACATCAATCTCGTATGC7880.9565312389992838No Hit
CTTATACACATCTCCGAGCCCACGAGACCTACATCAATCCTGTCTCTTATAC1420.17236984256078466No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTACATCA1340.16265886551510664No Hit
AGCCTAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1060.1286704458552336No Hit
ACGTGCGGGGCATTTTATGGTACTTCTTTCAAATTAACTGTCCAAGCCAGGA1010.12260108520168486No Hit
TGCTATGGGATTGACCGTCTTTTCCAGACCTCTTTAAGTCACACTCTTAACT980.1189594688095556No Hit
TACGAGGGAAACCAGACTATTTTAGAAACAAAAATAATGTTGAATTCATTAA980.1189594688095556No Hit
TCTCAAGGGCTTTTGGACAGTAATTTCTATCACAGTTAGAAGGAAATGATAG970.11774559667884585No Hit
GAGTAAGGGAGTGAAACTGAAACAAAATGAGGCACAGACAGTAAGAAAAAGA960.11653172454813611No Hit
GAACATGGGATAATTAAAAAGAACCAAGCAGAAATTCTAGAGTTGAAAAATG940.11410398028671659No Hit
CTGGGTGGGGAGAGAATATTCCAAGTAGAAAAGATAGCTTGAACCCAGAAGA910.11046236389458736No Hit
TGTATAGGGTTTCAAAAGAATTATGAACACACCTTTCCTGGATCACAGATTA910.11046236389458736No Hit
CATAAAAGGGCATGTGGTAGTGACTGGTGTCCCCTCTGTAATCTACCCTAGC900.10924849176387759No Hit
GCGTGGTGCTTCTTTAGTAAATACTGTACAGATTTTACCATGGAGAACTTTT880.1068207475024581No Hit
AGGTGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC860.1043930032410386No Hit
TATGGCGGGGATAGCTTGCTTTATTTCTGCCTTTACGTTGACAGTGTTGAAG860.1043930032410386No Hit
TCCTGTGGGAAGATTGAGCAATTTTACTTTTTTTTTTTTTTTTTTTTTTTTT850.10317913111032884No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)