FastQCFastQC Report
Sat 31 Dec 2022
SRR5846476.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846476.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145884
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTACATCAATCTCGTATGC6700.45926900825313266No Hit
CTTATACACATCTCCGAGCCCACGAGACCTACATCAATCTCGTATGCCGTCT6500.4455594856187107No Hit
CGTACTGGGCGACCAGGGAAAACCATCAAGCCAACAACAGGGTCTTGAGATA1890.12955498889528666No Hit
TACAACGGGGAAAAAAAACATGAGGTTAGGAAGAGTTATGAAGGACTAAATG1830.1254421321049601No Hit
TCGTGCGGGACGTGCCACACTTCTGTCAGCCTCCAAAGAGACATGCAGCCTT1810.12407117984151793No Hit
ACATCCGGGGACGCTCTTCCGATCTAGTGTTGGGGACTAGGATTTTGGTCAG1700.11653094239258588No Hit
TTGCTCGGGGATACTTCTAATCCTTTAAAGATTCCTCAATGAAAATATTTCT1690.1158454662608648No Hit
CTTTGTGGGCTGCCTGCTCAAAACTTCTCTCAATGCAATTCAGAGAGATAGA1660.11378903786570152No Hit
TTTGACGGGGGATACTCAGCGTCGCTGGCAGCCGCTGTACTTGGAAATTCGA1650.11310356173398041No Hit
GTGACAGGGAACTAATAACCATGCAATAGCCTCTCAGTACTCAAGTGAAAGG1650.11310356173398041No Hit
CTAACTGGGAGTTTCTTTCACCGCAGTGGGCTACCATAAACACATCACCTTG1620.11104713333881715No Hit
CATGGGGCTGTACATATCTTTATTTTTTCATTTGTGGCAAAATACACATAAC1610.11036165720709605No Hit
AAATGCGGGAGGAATGCCTACATTTCATATGGAGATCCAAAGAAGATCGTGG1610.11036165720709605No Hit
TGACATGGGAGACACTCTAGTCTACAAAATTATCCTAGGTTTAGCAATATTG1610.11036165720709605No Hit
AACACAGGGCTTCAAAAATTGGGCTTTCATCATGCTAGGACTACTATTCCTA1610.11036165720709605No Hit
TCTCTGGGGCGATCTTGAAGCGGGTGTCACAAGGTCTCCTAAGTTCAGTAGC1600.10967618107537497No Hit
AAGCGTGGGGGGGTCTTCACCAGTCACAGTCTGCTGACACCCTGACCCTGGA1570.10761975268021168No Hit
ACCATAGGGTAGACAATGTGGAATTACATAACAGGCATTGCACTAAAAGTGA1560.10693427654849058No Hit
GGTACTGGGATCACAGGGACTCTGTGGAGCAGGTAGCTGTGCTGGCGTCTTT1550.1062488004167695No Hit
TAGTCTGGGTAAAGTATTTATTCCAAATGCTTTCCAAATTCAAAATTTCATT1550.1062488004167695No Hit
GTACCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1530.1048778481533273No Hit
ACAAATGGGATATGGTTCCAAGTTGATTAAGCTTTGTTACCAGCAGACTATG1530.1048778481533273No Hit
ACTAACGGGAGCTTTGAAGCCAGGCATTGACTTCTCTCTAGCTACGAAAGTC1530.1048778481533273No Hit
GATGTTGGGCATTTTAACAATGTTTTCTCCTTGGGCTGGTGGTATATTACTG1530.1048778481533273No Hit
AGACCTGGGAGTTCTAGTACTCTTCTTTGTCAGTATATCAACCTACAGCTCC1520.10419237202160621No Hit
TTGTCAGGGAGGCTATAATGAATGCTAGTGAGGATGTGGAGAAAAGGGAAGC1520.10419237202160621No Hit
TACGGCTGGGAGCATTCAAAATCACCCCTAGAAAAAGTGAGTGTTTTAATGA1520.10419237202160621No Hit
AATGGTGGGGGTATCTAACTTTTAGCAAAGGAGGGTTGCAGAGCAGCTTAAA1520.10419237202160621No Hit
ACCACCGGGTTACTAACCAATACATAACCAGGAGCATTTAGAGAGCATCCAA1510.1035068958898851No Hit
TGATATGGGTGCTTTGCCAAAGTCATGAGTAATCAGTATAGGAAATGTAGTT1510.1035068958898851No Hit
AGTTCTGGGAAGAAGTGACAGACCACAGAGGAAAAACAGTGAAATTAGAACC1510.1035068958898851No Hit
GTTAATGGGGATGTCAAGGTAGCAACAAAACCCAAAAAATCCATCCTTTAGA1480.10145046749472184No Hit
TAGTACGGGACACCAGAGGTGGCCGAATCATCTCCACTTCTTCGCGTTCGGT1470.10076499136300074No Hit
AGAAGCGGGCATTATGTAACTTTTACCAGAAGTTGCCACTAGTTTATATTTG1470.10076499136300074No Hit
TCTCGGGGGAGCTCATCTCAATTTATACTGACAAGCTGGTAGACCTGAAAAA1470.10076499136300074No Hit
GCCCTCCTGTGACTTATTCCCTGAGACTTTTCAGGAGAGCCAGCCCACAGAT1470.10076499136300074No Hit
CCAACAGGGAGTCACAATGTTAAATGAACAAAATTCTTGAACAGTTTTTTTT1470.10076499136300074No Hit
ACTTTTGGGTGGGTATTAAACATATCTAGCATGGGGATGAAAATATCCGTGA1460.10007951523127966No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)