FastQCFastQC Report
Sat 31 Dec 2022
SRR5846475.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846475.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142685
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTACATCAATCTCGTATGCCGTCT6690.4688649823036759No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTACATCAATCTCGTATGC6490.45484809195080067No Hit
TCGTGCGGGACGTGCCACACTTCTGTCAGCCTCCAAAGAGACATGCAGCCTT1790.12545116865823316No Hit
GTTAATGGGGATGTCAAGGTAGCAACAAAACCCAAAAAATCCATCCTTTAGA1760.12334863510530189No Hit
AGACCTGGGAGTTCTAGTACTCTTCTTTGTCAGTATATCAACCTACAGCTCC1740.12194694607001438No Hit
CATGGGGCTGTACATATCTTTATTTTTTCATTTGTGGCAAAATACACATAAC1720.12054525703472684No Hit
TTTGACGGGGGATACTCAGCGTCGCTGGCAGCCGCTGTACTTGGAAATTCGA1690.11844272348179558No Hit
TACAAAGGGGGAGCATCTGTTCTAGTACTTTCATGTTGAAAGTGTTCAATAA1640.11493850089357677No Hit
CTCAACTGGGAGCCTCATCTGTAATGAAGGGTAACTCTCCAACCTGTCTATT1600.11213512282300171No Hit
AACAAGGGGTACCCTTGGAGATTGTTCTACAACCATTATCCCAGCATTGCTT1590.11143427830535796No Hit
AGTTCTGGGAAGAAGTGACAGACCACAGAGGAAAAACAGTGAAATTAGAACC1570.11003258927007042No Hit
TGACATGGGAGACACTCTAGTCTACAAAATTATCCTAGGTTTAGCAATATTG1570.11003258927007042No Hit
ACAGTGGGATCTACAAAACACGATAAAGTGAAGTGCAACAAAATAAGAGGTG1550.10863090023478292No Hit
GATGTTGGGCATTTTAACAATGTTTTCTCCTTGGGCTGGTGGTATATTACTG1530.1072292111994954No Hit
CGAAAAGGGGGTGGCCTGCGTTTTGGAGAAATGGAACGAGATTGTCAGATTG1520.10652836668185164No Hit
CCTTCCTGCTGCAGGTGTATGCGCCGCTGCCTGGCCGCCCGGACGCCTTCCA1510.10582752216420786No Hit
ACATCCGGGGACGCTCTTCCGATCTAGTGTTGGGGACTAGGATTTTGGTCAG1510.10582752216420786No Hit
TGCGCAGGGAAAGGATCTTGGGTTGAACCTTTAGCCATCTAGATTATTTATA1510.10582752216420786No Hit
CACTGGGGCATTCCACACCTTGGGCTCCCCAGATTCATGTCCTCACATGCAA1510.10582752216420786No Hit
CTGTTTCGGGTAGCTGCATTAGCCCCTAACAGGAGAGTCAGCCTGTCCTTTA1500.10512667764656411No Hit
GCTAAAGGGGTGTTCCCATTGCATGTATGAGATGTGCCTCCCAAAGAGCTTG1490.10442583312892034No Hit
TAGTCTGGGTAAAGTATTTATTCCAAATGCTTTCCAAATTCAAAATTTCATT1470.10302414409363284No Hit
TTGCTCGGGGATACTTCTAATCCTTTAAAGATTCCTCAATGAAAATATTTCT1470.10302414409363284No Hit
TTCTCAGGGGTGTTACTTATCAGCTATGATGAAATTACTAGAGTTTTTCCCA1470.10302414409363284No Hit
GAAGCAGGGAGGCACCCACCACCATGCCAGCTAATTCTTTGTATTTTTAGCA1460.10232329957598905No Hit
CGCTAAGGGAACCCATGCTTACTATAAACAAAGATAAAAATAACAGACTTTG1450.1016224550583453No Hit
ACTTATGGGGTAGACAAGTGAAGGCTAGAGTCAGAGAACCCACTATTGGACT1440.10092161054070155No Hit
GCCCTCCTGTGACTTATTCCCTGAGACTTTTCAGGAGAGCCAGCCCACAGAT1440.10092161054070155No Hit
ACTCTTGGGACTCCTTTCTAGGAACAATTGTATTAGTTATAGGTCTGTACTT1440.10092161054070155No Hit
GTCCCGGGGAAGAATGGCAATATTAAAAAAATTAAAAAATAATAGATGTCGG1430.10022076602305778No Hit
AGCTGTGGGTGGGCGGGGGGCGGGGATGTGAGCTTCATCAAAGAAGATTTTG1430.10022076602305778No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)