FastQCFastQC Report
Sat 31 Dec 2022
SRR5846474.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846474.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144385
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTACATCAATCTCGTATGC6670.46195934480728607No Hit
CTTATACACATCTCCGAGCCCACGAGACCTACATCAATCTCGTATGCCGTCT6190.4287148942064619No Hit
TCGTGCGGGACGTGCCACACTTCTGTCAGCCTCCAAAGAGACATGCAGCCTT2100.1454444713786058No Hit
TGACATGGGAGACACTCTAGTCTACAAAATTATCCTAGGTTTAGCAATATTG1950.13505558056584824No Hit
AATGGTGGGGGTATCTAACTTTTAGCAAAGGAGGGTTGCAGAGCAGCTTAAA1840.1274370606364927No Hit
TACAACGGGGAAAAAAAACATGAGGTTAGGAAGAGTTATGAAGGACTAAATG1780.12328150431138969No Hit
CTTTGTGGGCTGCCTGCTCAAAACTTCTCTCAATGCAATTCAGAGAGATAGA1750.12120372614883818No Hit
AAATGCGGGAGGAATGCCTACATTTCATATGGAGATCCAAAGAAGATCGTGG1740.12051113342798768No Hit
AGACCTGGGAGTTCTAGTACTCTTCTTTGTCAGTATATCAACCTACAGCTCC1730.11981854070713717No Hit
AGTTCTGGGAAGAAGTGACAGACCACAGAGGAAAAACAGTGAAATTAGAACC1730.11981854070713717No Hit
ACTTTTGGGTGGGTATTAAACATATCTAGCATGGGGATGAAAATATCCGTGA1640.11358520621948262No Hit
AACAAGGGGTACCCTTGGAGATTGTTCTACAACCATTATCCCAGCATTGCTT1600.11081483533608061No Hit
CATGGGGCTGTACATATCTTTATTTTTTCATTTGTGGCAAAATACACATAAC1590.11012224261523011No Hit
TTATTTGGGGTGATAAGGGCTGCATCCCTCCCTTGGTTCAAAGAGGCTTCCA1590.11012224261523011No Hit
GCGAGCGGGGCTGAGAACCAAAATGAACGAAAATCTGTTCGCTTCATTCATT1580.1094296498943796No Hit
TACGGCTGGGGTTGGGACATATGGAGACTGAAGGGCAAGAAGTATCTCCCAC1570.1087370571735291No Hit
TTGACAGGGTACTACCATATGGATGAATATCAGCAAATAGTAAATCTCTCAA1560.1080444644526786No Hit
TACGGCTGGGAGCATTCAAAATCACCCCTAGAAAAAGTGAGTGTTTTAATGA1550.1073518717318281No Hit
TACAAAGGGGGAGCATCTGTTCTAGTACTTTCATGTTGAAAGTGTTCAATAA1550.1073518717318281No Hit
CGAAAAGGGGGTGGCCTGCGTTTTGGAGAAATGGAACGAGATTGTCAGATTG1540.10665927901097759No Hit
TCTCGGGGGAGCTCATCTCAATTTATACTGACAAGCTGGTAGACCTGAAAAA1530.1059666862901271No Hit
GCAAGTGGGACCAACAGACACAGACATTTACACTTCTAGGCCAGGAAAGCGC1520.10527409356927658No Hit
AAATACGGGGAACTGATTGCTAGACATTTTTAAGCTAGTGTTGATTGATTGC1520.10527409356927658No Hit
TAGTACGGGACACCAGAGGTGGCCGAATCATCTCCACTTCTTCGCGTTCGGT1500.10388890812757558No Hit
GTTAATGGGGATGTCAAGGTAGCAACAAAACCCAAAAAATCCATCCTTTAGA1490.10319631540672508No Hit
ACTCTTGGGACTCCTTTCTAGGAACAATTGTATTAGTTATAGGTCTGTACTT1490.10319631540672508No Hit
CCTTCCTGCTGCAGGTGTATGCGCCGCTGCCTGGCCGCCCGGACGCCTTCCA1480.10250372268587457No Hit
TTCTCAGGGGTGTTACTTATCAGCTATGATGAAATTACTAGAGTTTTTCCCA1480.10250372268587457No Hit
ACAAATGGGATATGGTTCCAAGTTGATTAAGCTTTGTTACCAGCAGACTATG1470.10181112996502407No Hit
TAGTCTGGGTAAAGTATTTATTCCAAATGCTTTCCAAATTCAAAATTTCATT1470.10181112996502407No Hit
AGAAGCGGGCATTATGTAACTTTTACCAGAAGTTGCCACTAGTTTATATTTG1470.10181112996502407No Hit
ACTAACGGGAGCTTTGAAGCCAGGCATTGACTTCTCTCTAGCTACGAAAGTC1470.10181112996502407No Hit
TATACCAGGGTGAGCTGATGTCAATGCTATGGACTGCACAAAAGTCTCAATG1470.10181112996502407No Hit
AAATAGGGGGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG1460.10111853724417356No Hit
GCACATCGGAGGAAAACTCGGTAAAGCAAAATGATGTTGATATGTTGAATGT1460.10111853724417356No Hit
AAGTTCGGGGAGCAGGGGGACATGAATGAGAAACATCATTCCTTTGCAAGGA1450.10042594452332307No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)