FastQCFastQC Report
Sat 31 Dec 2022
SRR5846424.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846424.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141071
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTACATCAATCTCGTATGCCGTCT11180.7925087367354028No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTACATCAATCTCGTATGC10250.7265844858262861No Hit
AAGGTAGGGATTTAATGAGGAGTGTATCTTACCAAACAAAACAATGGAAATG2230.15807642959927978No Hit
GTTGCAGGGGGCTAGACTTAGATTCATTAACTCAAATTCAATGCTTCTATCA2200.15594984086027602No Hit
TTTAAAAAGAGATTAAACCGAAGGTGATTAAAAGACCTTGAAATCCATGACG2180.1545321150342735No Hit
TTCCACGGGGAGGAAATCAATCTCATTTACAATAACATCAAAAAATTGAAAT2120.150278937556266No Hit
AAGCACGGGAAGAATTGTTTAGGTAAAAGGTTTTTAACTACTTAAAAGAGAT2010.1424814455132522No Hit
ACGTTAGGGCTGGGTAGCTGGGATTACCGGGGCCTGCCACCACACTTTGCTA1960.13893713094824592No Hit
CAATACGGGCTTGTAATGTCTTTGTTAGAAGGAGTTTATATTAGGATTATGC1960.13893713094824592No Hit
TTAGGCGGGGCTGGAGGTGCATTTTAAGTTTTAATAATAAGTGCTAGAATGA1940.1375194051222434No Hit
TACAATGGGTGTAAGGCCATTAAATAAAAGCAGGATGACTTAGATATTTTTC1880.1332662276442359No Hit
CGGCTAGGGCACCTAACCTCTTATTGAAAGTAATTCAAAGTTATTTTCAAGC1880.1332662276442359No Hit
TATTTTGGGCACTCAGTCACCATGGCTCCGTCTGTCAAACTCAACTCTTTTT1880.1332662276442359No Hit
CCATTAGGGAGCCAACGTCACTCACTAGGGAAAGGCAATTGGTAAATCCACT1870.13255736473123464No Hit
GACACCGGGTGATGCGGGGCTGTGAATAAAAGGCTTACATTTGTTCCTGCAT1870.13255736473123464No Hit
AACTCCGGGTGGACTGTGGATACTGAATCAGTGTACTATTGGCTGCAGAATT1870.13255736473123464No Hit
ATGAGCGGGTAAGGGAGAAGAAGAGGTGAGAGTTTGTCAGTTGGGTTTTGTT1870.13255736473123464No Hit
AACTCCGGGAAGTGAGATCGTGCAGTATTTGTATTTCTGTGCTTGGCTTATT1860.13184850181823338No Hit
GTAGTAGGGTATAACTCTTACTAACAATGTATAACAATTTGGGAAGAAGTTA1850.13113963890523211No Hit
ATTCAAGGGTGCTACCAAATGGCAAAAAGCAACAATAAACAGTTTGATTTTT1840.13043077599223088No Hit
GAAACCGGGTGAGACATGCATTTTCACGTGTGGCCAATTGCACAGATGCGCA1830.12972191307922962No Hit
GCGACAGGGATGTAAGGTAGACATTTAAAATACTTCCATATTTTATGGTTGC1820.12901305016622835No Hit
ACACACGGGATTCAACCTGAGAAAACTGATTAAAAAATTAGTTTAAATTTGC1810.1283041872532271No Hit
GTTAAGGGGTAAAAAGAATGAAAAAGTGCTTTTTCCTCAACTTGATAACTGT1800.12759532434022586No Hit
ACGCCAGGGTTATGATACTGATTTTCCTTTAATGTTAATATGCCAGAAAGCA1800.12759532434022586No Hit
CATCAAGGGGATTTGTTGGAGTATGATATTTGCTGACAGCACTATATACTGG1800.12759532434022586No Hit
GCAAGAGGGCAGATAAAGTTACTAGAGTACTGTATATACATTTTCCTTTCTA1770.12546873560122207No Hit
AGAAGCGGGATTTCCTTTCCTTTCCTTTGTAAAACATGCTCAGCAAACTGCA1770.12546873560122207No Hit
AGCTAGTGGGATTATACTAGAAGAAGAAACCAGATCACTCCACCCCTCATCA1760.12475987268822082No Hit
CGACACGGGATTGTGCCCGGTCGGAGTAAACACAGAATGCTATCGAATCTAT1750.12405100977521957No Hit
GCATGGGCTGCCAGTAGCGTAACAGTCATATTTGCTTTTATTCACATTCACC1740.12334214686221831No Hit
CGTCAAGGGATTCCTCCTCCCCCATCATAACCATATTCAGCACCCATTTTTA1740.12334214686221831No Hit
ACTAGAGGGATTTGAAGTCCCTGGCACCTACAGCTTCAGAAAACATTAAACA1730.12263328394921706No Hit
GCCTCGGGCAGAGCTAATATTTTTAGAGTAACTGATCTGGATCAATGATCTA1730.12263328394921706No Hit
AGCTACGGGAACCAAACTGACTTACTGAGTCTTAGTGGAAAAACACTTTGTG1720.1219244210362158No Hit
TAAATGGGGTGTTCTTGTCTTTTACATTACAAAGGTTGGTTTAAAGCCAGGC1710.12121555812321455No Hit
CGATGGGTAAAAATATAGGTTTTCCAGCACTGGGACTATGAAATTTGAGATT1710.12121555812321455No Hit
GACACTACATTAGGAATTGGATTGTTTGGGTTTGCTTTGTGTTTTTTGAGGT1700.12050669521021329No Hit
ACTGTTGGGATCTAATGGAATACTGTGTGGTCATTTAAATTTATATCATGGA1690.11979783229721204No Hit
CGAGTAGCGGGGAAGCTCTTGGGATCCTAGAAGTATTCTTATTTTCTTCTCT1690.11979783229721204No Hit
GTCGCAGGGGTTTGTATGATTTTTGTTTTCAATACATGTGATGGGTTACTGT1690.11979783229721204No Hit
TTTGATGGGCAACAGCCTTTACTGCCATTTTTTTTTAAATGTTTTCACTCAG1690.11979783229721204No Hit
ATACAAGGGTTATATGGCAAATAGCATCATCTTGAATTTTGTGAAATTATTT1680.11908896938421078No Hit
TACTTAGGGACAGGACGTATTCTTCCATCGTCTTATATATAACAAGCTGTTT1680.11908896938421078No Hit
CCATAGGGGTAACACTATTTCGGCTGGTCAAGAGGATTTCCCATCTGTCCTG1670.11838010647120953No Hit
AGGAAAGGGAACCCTGATGATGATGTCAAGGGTCTTCTTCTGCTGTGTGGGC1670.11838010647120953No Hit
ACTACGGGGAAGAATGGTTCTAATTCACAGAGGTCTATTAGGCTGTGTTTAC1660.1176712435582083No Hit
CTCAACGGGATTAGCAAGTCAACAATGGCTTAGAATGATACAAACACTACAT1660.1176712435582083No Hit
CTTACAGGGATTCAACAAAACCTTCAGGAAGCCCTTTGGAAGAAAAACACGA1660.1176712435582083No Hit
GTTGCTGGGAAGGTGTTAGCTGCATTCACAACCAGTCTAATCATTTGTCTCA1660.1176712435582083No Hit
CATACAGGGCAAATGGCAAAAAGGGAATTGTCATAGTTACTAATTGTATTGT1650.11696238064520703No Hit
GATAATGGGGCCCGCGGGGGGGACCATGTGCCTGTGTCCCACGAGCAGCCGA1650.11696238064520703No Hit
TGCTCTGGGGATACTGGCAAGCCCTCTGGGTGTCCAGATCTCATGCAAACAC1650.11696238064520703No Hit
ATTAATGGGGACAACAAAAAAATTTCTGTAATTTGACCTTATAGTGCTATAT1650.11696238064520703No Hit
TTGAGGGGATAAAAGAGTGGCTTTAAAATTTGAAATACTGGGAAAAATATTT1610.11412692899320201No Hit
GGACCCTCAATACCTACTTAACCTCATCTATTTCCGTTTGTCCTTTTGCCAG1610.11412692899320201No Hit
GCCAATGGGCAGGATATCACACCACGTCTTTTCAAAAACTAAAGAGAATTCA1610.11412692899320201No Hit
TCTTATGGGAGGAGAAGTTTGGAATGCACTTAATAGCTGGTTTTTACACCTT1600.11341806608020076No Hit
AGCTCGGGCTACTTGCTGATTGTACCTATTAAATATTTTATATTCAGTCAAG1600.11341806608020076No Hit
TTGTAGGGGGTAATAAGACTTGTAACATTTTCAAGAAATCAAGTTTAAAGTC1600.11341806608020076No Hit
GTTGCAGGGATAGTTATTAAAGTGAACTTATCTATCCTTTTGTTTAAACATT1600.11341806608020076No Hit
ACCAGCGGGCTCTAGCAGGGGGAATTAGTCCGAGTGGAGAGAGCGAGCTGAG1600.11341806608020076No Hit
TTCCTAGGGAACTTGAAGGACTGTAGGAGGTAGAGGAACATGGAAAATTGAA1590.1127092031671995No Hit
AGTTTTGGGGATGCATCTGCAGCCAGAGTCGCACTAGGAATCACGACGGTGC1590.1127092031671995No Hit
GCTGCCAGGTACTGTTAGATTCCAAAAATAAAAGGAAAAAATAATTATACAC1590.1127092031671995No Hit
AAGTCGGGGAACTTTATACTGAATTTATTCCCCAGTTTTTATAGCTTAATAA1590.1127092031671995No Hit
ACATGAGCCACTGCACCCGGCCCTTCCTCTCCTTTTTAAAGAGTATTAGTAC1590.1127092031671995No Hit
TATTGCGGGTGTCTTTGGAATACTAATACAAAAATTGTGTTAATGCTACTTT1590.1127092031671995No Hit
GTTCTTGGGCGCCTCACACTCATTCTCAACCCCCTGACAAAACACATAGCCT1590.1127092031671995No Hit
GTAGTAGGGAAGACACAGGCACACAGAGACTTATTCAAAAGATCATAGAACA1580.11200034025419825No Hit
CTGATTTCCCTAGCAATGATTCTGTTTCCATTGATTCAAATTACTAAAAATT1580.11200034025419825No Hit
ACGCGGGGGACCTACATTGGTCCTTTGTCTGTATGTCAAATGCATTATTTTA1580.11200034025419825No Hit
TTTGCCGGGGTGGCTTTGCAACCATTTTCATTTGTTTGTTTTCCTTTTATTT1580.11200034025419825No Hit
CTTTCAGTCTGCTAAACCAAAACCAGCAAAGAAAACAAATCCCTTTCCTCTC1570.11129147734119699No Hit
TAGAATGGGGTGTATAGTGAGAGCACCTCACTGGCAGAAAAACTGCTTTCCC1570.11129147734119699No Hit
TCACGTGGGTCTACGGTGGGGATCTCCTCCCAGAACGTCACAGTGCTCAGAG1570.11129147734119699No Hit
TATTGTGGGAGAGTGGAATGGATTGGAACGGGGAGGTGTGAATAGCAGGTCA1570.11129147734119699No Hit
AGCTATGGGGGCTTACAAGAAGTAACTTTAGGTAGGAGCTGTTTTTGTTTGA1560.11058261442819574No Hit
AGTTACGGGGAACAAACAAACAACAAACCACAAACACATAAGAGCACTCCGG1560.11058261442819574No Hit
CACTAAGGGTACTAAAGCAAAAATAATTTAGGGTCTTATCCTGGGTAAAATA1560.11058261442819574No Hit
GGTAGTGGGATGTCTATCAGAAGAAACATCTGGTTGAAATTTTGTCATACCA1550.10987375151519448No Hit
ATAAGAGGGTGATATCCAGATATGCGTACTTCAGCTAATATTTTATAGCCTT1550.10987375151519448No Hit
CGTTTTGGGGTAGGTCCTCTTGGGATTGCTAGTTTGTTCTTGCAGTCCATCT1550.10987375151519448No Hit
TTAATGGGTATTCACATCTCTTTTTAAGACGAAGTATGCAAACATTTGTTTC1550.10987375151519448No Hit
AATCAGGGGAGCCTTTGAGAACCTGTATAAGAATACGTAAGTAATCCAGAGC1540.10916488860219323No Hit
GCAGCCGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1540.10916488860219323No Hit
AATTCCGGGGTATTTGTCATGTATAACACAATATTTTGAAGTACAGTCCTGT1530.10845602568919196No Hit
CCAAAGGGGGAGAGAAAGCATTTTCCAAGCCAGGTTCACTTGGCTGCAAAGC1530.10845602568919196No Hit
TTTCGAGGGCAGGGCAATGAGAAAATTAGATGTGTAATATATCTTTCATCAA1530.10845602568919196No Hit
ACTGCAGGGATCATAGCAGGGATATATGCTAATGTGGAGAGCAGATAAAAAG1520.10774716277619072No Hit
TGGGTTGGGTCTGCAAAGCCTCGCTGAGGAAATTGACTTTGAATATCCTTTT1520.10774716277619072No Hit
GTTATAGGGGCGTAGGGGAAGCTTCTTCATGGAGTAAAAAATGTATTTAAAA1520.10774716277619072No Hit
ATCTCTGGGAGAATCAGAAGACCTACTGCTGTTTGCACCTTAAAAAACTAAA1520.10774716277619072No Hit
CCGAACGGGCCAGAGAAGGTGGTGCCACCTCCTCTTGTAGATAAATCTGCTG1520.10774716277619072No Hit
ACAGCGGGCCGATCTACGTTGGGGGTGCTTCTTTCTTTGGGAATATTTGAAT1520.10774716277619072No Hit
TGAAACGGGTACGTGGAATATCTTTTTCCACCCTACCACTTTCAATCTGCTT1510.10703829986318945No Hit
CACACGGGGATCGGACTTATTCACGCAGTGGCCAATAATCAAGACAAACTGG1510.10703829986318945No Hit
ACAGTAGGGGCTTCATCTTCTCTCAGGACCCACAGAGCTCAGATGAGGTCAA1500.1063294369501882No Hit
TGTCGCGGGTCCGATCTAGTGGGGGGATACAGTACAGGTAATGAGTAATTTT1500.1063294369501882No Hit
TATTTAGGGATGGTTATTTAAAAGAATGTCTTTGTTTCTAAGAGACCCATGC1500.1063294369501882No Hit
AAATTGGGGAAACAATTATGTGCACTTTGATACACAACCTTCTCTAACCAAC1500.1063294369501882No Hit
GACACAGGGACTGGGCGGTTAGATCGCAAGGTCTACTAAGCTTTGGCCAGAC1500.1063294369501882No Hit
CATTGCGGGACTTACCTAAATCCTGGTAATAACAAATGGTTTTTGTAAAGTG1500.1063294369501882No Hit
GCTATGGGGGGGACATTTTAGAGATTAGGATCCCCAGACAAGTAGTGATGTT1500.1063294369501882No Hit
TGAACGGGACTGACCTTTCAGGCAATGGCATATGATCTACTACCAATTGAGA1500.1063294369501882No Hit
GTTTGAGGGATACGAAAATTAGCTTTTTGTCCTTTTTATTTAAAAATGTTTT1500.1063294369501882No Hit
TGCAGGGAATCATCTGTGTTTGTTTATTAAACATATGTCACTTTGAAATGCT1490.10562057403718694No Hit
CCCAGGGCATATAAAGAAATAAAAAGAAGTCTCAGTTGCAGCTATTTGTCAA1490.10562057403718694No Hit
AAGAGGGGTTGCAAGTATTCAACTCTTCCTTTGTAGGGCAAAAGCAGCCATA1490.10562057403718694No Hit
CTGCGGGGCATTTTCCTGTAGTTTAGCTTCGTTGAATTTTTTTTGACACTGG1490.10562057403718694No Hit
TCTGGAGGGCTAAGGATAGGGATCTGAGCAGGTTCCTGAGTACTTGAAAGGC1490.10562057403718694No Hit
GTAGGTGGGAGAGGTGGGGAAGAAAATAATTTAGAAAGCCTACATCGACTTT1490.10562057403718694No Hit
CTAATAGGGATAATAACCAAGAGCTCTATGAGTCTTGTTTTTGGTTTTTAGT1480.1049117111241857No Hit
ATCCTGGGGTAAACAGTCATGTAACCCCTTTCAACTGTGTGCTTTTCCTAAT1480.1049117111241857No Hit
TCGTACGGGGATCTAGGACAGGGGTGAACAGGCATCCTAAAGAATGAGAGAA1480.1049117111241857No Hit
CACACTGGGATATGTTGTAATTTATGATTCCTGGTTATGTATTTTTGTGGGA1480.1049117111241857No Hit
CAGATAGGGTGGGTTTGTCCAGCTCAGGTAGCTCTAGGAGCACCATCCTGCC1480.1049117111241857No Hit
TGGCTCGGGTTGATTGAAGTATATTGTCATATAATAAAGTGCCTGTTATTTA1470.10420284821118443No Hit
TATGCCAGGGCCAGGGGATAAACTCTGACTTAGTCTTGGAGTGGCAGATACA1470.10420284821118443No Hit
GTTGTAGGGGTAACTGCTGTAGCTGAAGCTACTGGAAGATTTGACTGAGTTG1460.10349398529818318No Hit
GCATAAGGGCTTGTTTTCCCCAACATCTGGGATAAATAGAGCTGCTTGTATG1460.10349398529818318No Hit
CTTTATGGGAAGTGAGGGTACATACAGCATTTCACTGTGTTTTTAGTGTTGA1460.10349398529818318No Hit
GATTCTGGATAGGCATTCTCATTGAGCTCTTGTTTTAATTGATATGTTTTTA1460.10349398529818318No Hit
GCGTAGGGGAAGAAAGCCAGACTTTGGAAAAGGATAATTGGAATAAAGGGAG1460.10349398529818318No Hit
CTCGCAGGGTCCAACAGTTTAAGGTACTATACCACATGGTAACCTACTGTTT1460.10349398529818318No Hit
CTTCCTGGGGCGTTAGCAGCCATTGAGGACTTCTTCCTAGAGCATGAAGCTC1460.10349398529818318No Hit
ACTCCAGGGGTTTTATAAATTCATATTTACTGTCCTGATCTAGCCACTATCA1460.10349398529818318No Hit
GCGCTACGGGACTTAAGTAAAATAAAGGCACTAATAAAGATAAGAGCAGAAA1450.10278512238518192No Hit
ATCCGGGGGGGACTCACATGAAGTTGCAAAATTAATAGTGAGAGTCTCATGA1450.10278512238518192No Hit
ACAGGTGGGGAGTCTTCCAGGTGTGACAATCAGGATATAGAAAAACAAACGT1450.10278512238518192No Hit
TAACACGGGAACCTTGCTAAACTCTCCTGTTAGACCTGGTAAATCTCTGATA1440.10207625947218067No Hit
TGAGAAGGGCTGAATTTGCCTATTCCAAATATTTTGTATAAGTGGAATCATA1440.10207625947218067No Hit
ATGAATGGGCTAGTTGTTTCTTGAAATCAAGCTGGCACGTGTGACTCCAGGA1440.10207625947218067No Hit
CCATATGGGATGATTAAATTAGGACATAAGAGAAACTCAGCAGATCAGGGGG1440.10207625947218067No Hit
AATTGCGGGGACCCAGTGGTAATTTAGGAGCATGTTGTTTAATTCCCATTAT1430.10136739655917941No Hit
CTGTTAGGGGCATTCAGAAGACTTTTATCAAACTTATTAACCTATTTTCCTT1430.10136739655917941No Hit
AAGTTTGGGATTGAGATATGATTCAAATTTCATAAAATCTCATAAAATCCTA1430.10136739655917941No Hit
CTATAGGGCTATCAGAATATTACCTTGTGTTCATAGGTTAACTTGTTTTTGT1430.10136739655917941No Hit
GCAATGGGGGCAGGAAGAGAGACCTGACTGACATGTATATACTCTTGCCCTC1420.10065853364617816No Hit
ATCTCAGGGGAAATGTAGAGATGAATGAGATAAAAGAGGCCCAAGCTTGGAC1420.10065853364617816No Hit
TCGTGGGGTTTTGTAGTTAATTTATTAAGTCTGGGATGTAGAACTTCAAGAA1420.10065853364617816No Hit
TGCTTTGGGGTATAGCAAAACATGAAAAGCACTGCATTACTGTGATAAAAGT1420.10065853364617816No Hit
CGCTGAGGGATTCAGTTTAGGGGGAAGTGGGTTGGGAGTAGTGGTAAAGATG1420.10065853364617816No Hit
TCTACTGGGACCTCAGAGGCAGAGGAACACCACCAGCTCTTCGATCTGATTG1420.10065853364617816No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)